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Literature summary for 3.2.1.51 extracted from

  • Yano, T.; Yamamoto, K.; Kumagai, H.; Tochikura, T.; Yokoyama, T.; Seno, T.; Yamaguchi, H.
    Purification and characterization of a novel alpha-L-fucosidase from Fusarium oxysporum grown on sludge (1985), Agric. Biol. Chem., 49, 3179-3187.
No PubMed abstract available
Search for more literature for 3.2.1.51

Inhibitors

Inhibitors Comment Organism Structure
alpha-L-fucose 85% inhibition at 2.5 mM Fusarium oxysporum
D-arabinose 95% inhibition at 2.5 mM Fusarium oxysporum
Hg2+ marked inhibition at 20 mM Fusarium oxysporum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.87
-
 4-nitrophenyl-alpha-L-fucopyranoside
-
 Fusarium oxysporum

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular most induction of enzyme synthesis if cells grown in 2% active sludge hydrolyzate medium Fusarium oxysporum
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 gel filtration Fusarium oxysporum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
saliva + H2O Fusarium oxysporum Ulex europaeus alpha-L-fucose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Fusarium oxysporum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
homogeneous, chromatography steps Fusarium oxysporum

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Fusarium oxysporum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.58
-
-
 Fusarium oxysporum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
 Fusarium oxysporum 4-nitrophenol + alpha-L-fucose
-
 ?
saliva + H2O Ulex europaeus Fusarium oxysporum alpha-L-fucose + ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Fusarium oxysporum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 stable up to Fusarium oxysporum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 5.5
-
 Fusarium oxysporum

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 8 stable within Fusarium oxysporum