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Information on EC 2.1.1.56 - mRNA (guanine-N7)-methyltransferase
for references in articles please use BRENDA:EC2.1.1.56
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EC Tree 2 Transferases
2.1 Transferring one-carbon groups
2.1.1 Methyltransferases
2.1.1.56 mRNA (guanine-N7)-methyltransferase
IUBMB Comments
The terminal N7-methylguanosine facilitates gene expression in eukaryotic cells and is recognized by cap-binding proteins.
The enzyme appears in viruses and cellular organisms
- 2.1.1.56
- dengue
- viruses
- influenza
- flavivirus
- pandemic
-
sars-cov
- coronaviruses
- exoribonuclease
- encephalitis
- mtases
- covid-19
-
anti-ns1
-
guanylyltransferase
-
replicase
-
proofreading
-
mosquito-borne
-
denv-1
- rig-i
-
arthropod-borne
-
anti-denv
- coronaviridae
-
chikv
- sinefungin
- remdesivir
- gpppg
-
ns1-specific
-
anti-dengue
- bluetongue
- medicine
- analysis
- drug development
- synthesis
Synonyms
non-structural protein 1, nsp14, cap methyltransferase, n7-mtase, mrna cap methyltransferase, nonstructural protein 14, tbcmt1, n7-methyltransferase, tbcgm1, guanine-n7 methyltransferase, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
(guanine-N7)-methyltransferase
cap methyltransferase
cap N7-MTase
cap-methyltransferase
guanine-7-methyltransferase
-
-
-
-
guanine-N7 methyltransferase
messenger ribonucleate guanine 7-methyltransferase
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-
-
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messenger RNA guanine 7-methyltransferase
-
-
-
-
methyltransferase, messenger ribonucleate guanine 7-
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-
-
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mRNA cap (guanine N-7) methyltransferase
mRNA cap (guanine-N7) methyltransferase
mRNA cap methyltransferase
N7 methyltransferase
N7 MTase
N7-MTase
NS5
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the nonstrucutural protein NS5 has dual functions as both an N7 methyltransferase and a nucleoside 2'-O methyltransferase
NS5MTase
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the protein contains mRNA cap (guanine N-7) methyltransferase and (nucleoside-2'O)-methyltransferase activities
nsp14
RNA N7-MTase
RNMT
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA]
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA]
subunit interaction in vitro and in vivo is a necessary condition for full enzyme activity
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S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA]
RNA triphosphatase, RNA guanylyltransferase, and RNA (guanine-N7-)methyltransferase form the mRNA capping enzyme complex consiting of a small and a large subunit
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S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA]
RNA triphosphatase, RNA guanylyltransferase, and RNA (guanine-N7-)methyltransferase form the mRNA capping enzyme complex consiting of a small and a large subunit
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S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA]
RNA triphosphatase, RNA guanylyltransferase, and RNA (guanine-N7-)methyltransferase form the mRNA capping enzyme complex consiting of a small and a large subunit
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA]
substrate binding sites
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S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA]
active site is located at His682 and Tyr683
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S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA]
R may be guanosine or adenosine
-
-
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S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA]
lipid-binding site, membrane binding activity of wild-type and diverse mutants
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S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA]
in-line mechanism of methyl transfer without direct contact between the enzyme and the N7 atom of guanine, which is the attacking nucleophile, the methyl carbon of S-adenosyl-L-methionine, or the sulfur of S-adenosyl-L-methionine/S-adenosyl-L-homocysteine, enzyme-ligand structures, active site structure
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
methyl group transfer
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PATHWAY SOURCE
PATHWAYS
MetaCyc
mRNA capping I
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:mRNA (guanine-N7)-methyltransferase
The terminal N7-methylguanosine facilitates gene expression in eukaryotic cells and is recognized by cap-binding proteins.
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CAS REGISTRY NUMBER
COMMENTARY
56941-25-4
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA]
S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA]
S-adenosyl-L-methionine + G(5')pppA
S-adenosyl-L-homocysteine + m7G(5')pppA
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + G(5')pppG
S-adenosyl-L-homocysteine + m7G(5')pppG
Substrates: and dGTP, best substrates
Products: -
Products: -
?
S-adenosyl-L-methionine + GDP
S-adenosyl-L-homocysteine + m7GDP
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + Gppp-RNA
S-adenosyl-L-homocysteine + m7Gppp-RNA
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + GpppA-RNA
S-adenosyl-L-homocysteine + m7GpppA-RNA
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + GpppAC3
S-adenosyl-L-homocysteine + m7GpppAC3
-
Substrates: is efficiently methylated at the guanine-N7 position
Products: -
Products: -
?
S-adenosyl-L-methionine + GpppGAU-[RNA]
?
-
Substrates: strong activity
Products: -
Products: -
?
S-adenosyl-L-methionine + guanylimidodiphosphate
?
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + guanylylimidodiphosphate
?
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + m7G(5')pppG
S-adenosyl-L-homocysteine + ?
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + mGpppGAU-[RNA]
?
-
Substrates: strong activity
Products: -
Products: -
?
S-adenosyl-L-methionine + mGpppGmAmU-[RNA]
?
-
Substrates: strong activity
Products: -
Products: -
?
S-adenosyl-L-methionine + mGpppGmAU-[RNA]
?
-
Substrates: strong activity
Products: -
Products: -
?
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA]
S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA]
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA]
S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA]
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA]
S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA]
-
Substrates: the enzyme methylates the guanosine cap on the N-7 position to create the cap structure, m7G(5)ppp(5)X
Products: -
Products: -
?
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA]
S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA]
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA]
S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA]
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + dGTP
S-adenosyl-L-homocysteine + m7dGTP
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + dGTP
S-adenosyl-L-homocysteine + m7dGTP
Substrates: and G(5')pppG, best substrates
Products: -
Products: -
?
S-adenosyl-L-methionine + G(5')pppN
S-adenosyl-L-homocysteine + m7G(5')pppG
-
Substrates: N: G
Products: -
Products: -
?
S-adenosyl-L-methionine + G(5')pppN
S-adenosyl-L-homocysteine + m7G(5')pppG
-
Substrates: unmethylated cap analogue, serves as in vitro substrate
Products: -
Products: -
?
S-adenosyl-L-methionine + G(5')pppN
S-adenosyl-L-homocysteine + m7G(5')pppG
-
Substrates: N: G
Products: -
Products: -
?
S-adenosyl-L-methionine + G(5')pppN
S-adenosyl-L-homocysteine + m7G(5')pppG
-
Substrates: N: G
Products: -
Products: -
?
S-adenosyl-L-methionine + G(5')pppN
S-adenosyl-L-homocysteine + m7G(5')pppG
-
Substrates: N: A
Products: -
Products: -
?
S-adenosyl-L-methionine + G(5')pppN
S-adenosyl-L-homocysteine + m7G(5')pppG
-
Substrates: TbCmt1
Products: -
Products: -
?
S-adenosyl-L-methionine + G(5')pppN
S-adenosyl-L-homocysteine + m7G(5')pppG
-
Substrates: N: A
Products: -
Products: -
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: -
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: essential and defining step in the eukaryotic mRNA synthesis
Products: -
Products: -
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: enzyme contains the conserved S-adenosyl-L-methionine-binding motif VLDLGXGXG, structural features for cap guanine specificity
Products: -
Products: -
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: mRNA, synthetic polyribonucleotides terminating in a dinucleoside triphosphate
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: specific for mRNAs with 5'-terminal sequence G(5')pppA- and G(5')pppG-
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: R may be guanosine or adenosine, methyl acceptors are unmethylated vaccinia virus mRNAs
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: active also on E. coli tRNA
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: free G(5')pppG is utilized, G(5')ppG is a poor substrate, while GTP, GDP, and G(5')ppppG are no substrates
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: essential for cell viability
Products: -
Products: -
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: -
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: unmethylated mRNA of reovirus and poly A(+) RNA
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: associated with mRNA-guanylyltransferase
Products: -
Products: -
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: methylates also undermethylated poly(A)-rich mRNA
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: methylates also undermethylated poly(A)-rich mRNA
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: -
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: in infected BHK cells
Products: -
Products: -
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: -
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: acceptor substrate specificity, overview
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: transfers methyl group from S-adenosyl-L-methionine specifically to position 7 of terminal guanosine
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: utilizes GMP, GDP, guanosine, ITP as substrates
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: mRNA, synthetic polyribonucleotides terminating in a dinucleoside triphosphate
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: specific for mRNAs with 5'-terminal sequence G(5')pppA- and G(5')pppG-
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: R may be guanosine or adenosine, methyl acceptors are unmethylated vaccinia virus mRNAs
Products: -
Products: -
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: R may be guanosine or adenosine, methyl acceptors are unmethylated vaccinia virus mRNAs
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: the presence of the small one is required for methylase activity, the large subunit bears functional domains for binding of the small subunit and for methylase activity
Products: -
Products: -
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: guanylyltransferase and RNA-triphosphatase are located on the large subunit
Products: -
Products: -
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: one of a series of three reactions in capping of vaccinia virus mRNA catalyzed by one enzyme, which is a heterodimer: no methylase activity with one of the subunits alone
Products: -
Products: -
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: R may be guanosine or adenosine, involved in synthesis of methylated capped structure at 5'-terminal of viral mRNA
Products: -
Products: -
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: transfers methyl group from S-adenosyl-L-methionine specifically to position 7 of terminal guanosine
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: acceptor substrate specificity, overview
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: utilizes GMP, GDP, guanosine, ITP as substrates
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: mRNA, synthetic polyribonucleotides terminating in a dinucleoside triphosphate
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: specific for mRNAs with 5'-terminal sequence G(5')pppA- and G(5')pppG-
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: R may be guanosine or adenosine, methyl acceptors are unmethylated vaccinia virus mRNAs
Products: -
Products: -
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: -
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
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Substrates: R may be guanosine or adenosine, involved in synthesis of methylated capped structure at 5'-terminal of viral mRNA
Products: -
Products: -
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S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
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Substrates: R may be guanosine or adenosine, methyl acceptors are unmethylated vaccinia virus mRNAs
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
Products: R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
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S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
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Substrates: -
Products: -
Products: -
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S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
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Substrates: -
Products: -
Products: -
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S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
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Substrates: -
Products: -
Products: -
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S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
Substrates: -
Products: -
Products: -
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S-adenosyl-L-methionine + GpppA
S-adenosyl-L-homocysteine + m7GpppA
Substrates: -
Products: -
Products: -
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S-adenosyl-L-methionine + GpppA
S-adenosyl-L-homocysteine + m7GpppA
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Substrates: -
Products: -
Products: -
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S-adenosyl-L-methionine + GpppA
S-adenosyl-L-homocysteine + m7GpppA
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Substrates: -
Products: -
Products: -
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S-adenosyl-L-methionine + GpppA
S-adenosyl-L-homocysteine + m7GpppA
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Substrates: TbCmt1
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S-adenosyl-L-methionine + GpppAC4
S-adenosyl-L-homocysteine + 7mGpppAC4
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Substrates: -
Products: -
Products: -
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S-adenosyl-L-methionine + GpppAC4
S-adenosyl-L-homocysteine + 7mGpppAC4
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Substrates: -
Products: -
Products: -
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S-adenosyl-L-methionine + GpppG
S-adenosyl-L-homocysteine + m7GpppG
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Substrates: TbCmt1
Products: -
Products: -
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S-adenosyl-L-methionine + GpppG
S-adenosyl-L-homocysteine + m7GpppG
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Substrates: -
Products: -
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S-adenosyl-L-methionine + GTP
S-adenosyl-L-homocysteine + m7GTP
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Substrates: -
Products: -
Products: -
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S-adenosyl-L-methionine + GTP
S-adenosyl-L-homocysteine + m7GTP
Substrates: -
Products: -
Products: -
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S-adenosyl-L-methionine + GTP
S-adenosyl-L-homocysteine + m7GTP
Substrates: -
Products: -
Products: -
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S-adenosyl-L-methionine + GTP
S-adenosyl-L-homocysteine + m7GTP
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Substrates: viral, not host enzyme
Products: -
Products: -
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S-adenosyl-L-methionine + GTP
S-adenosyl-L-homocysteine + m7GTP
Substrates: -
Products: -
Products: -
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S-adenosyl-L-methionine + GTP
S-adenosyl-L-homocysteine + m7GTP
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Substrates: -
Products: -
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additional information
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Substrates: enzyme sequentially methylates the guanine N-7 and ribose 2'-O positions of viral RNA cap. The order of two methylations is determined by the preference of 2'-O methylation for substrate m7GpppA-RNA to GpppA-RNA, and the 2'-O methylation is not absolutely dependent on the prior N-7 methylation
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additional information
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Substrates: does not methylate ATP, CTP, UTP, ITP, or m7GTP
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additional information
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Substrates: does not methylate ATP, CTP, UTP, ITP, or m7GTP
Products: -
Products: -
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additional information
?
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Substrates: no substrates are poly-(A) terminating in a single phosphate or a diphosphate
Products: -
Products: -
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additional information
?
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Substrates: the enzyme is responsible for guanine-N7 methylation of recapped mRNAs
Products: -
Products: -
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additional information
?
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Substrates: no substrates: ATP, CTP, UTP, dATP, dCTP, dUTP or cap analog m7GpppA
Products: -
Products: -
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additional information
?
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Substrates: no substrates: ATP, CTP, UTP, dATP, dCTP, dUTP or cap analog m7GpppA
Products: -
Products: -
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additional information
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Substrates: no activity with 7mGpppA
Products: -
Products: -
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additional information
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Substrates: the protein reveals cap-independent methyltransferase activities targeting internal adenosine residues. Besides this, the enzyme domain also methylates the N7 position of the cap guanosine. The enzyme preferentially methylates adenosine residues and is not active on poly G, C and U RNAs
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additional information
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Substrates: ATP, XTP, CTP, UTP, and guanosine containing compounds with phospho groups in either position 2' or 3' or in 3'-5'phosphodiester linkage
Products: -
Products: -
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additional information
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Substrates: isolated methyltransferase domain shows a lower but kinetically equivalent activity than the complete enzyme, which is highly enhanced by association with the D12 subunit
Products: -
Products: -
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additional information
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Substrates: isolated methyltransferase domain shows a lower but kinetically equivalent activity than the complete enzyme, which is highly enhanced by association with the D12 subunit
Products: -
Products: -
?
additional information
?
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Substrates: no substrates are poly-(A) terminating in a single phosphate or a diphosphate
Products: -
Products: -
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additional information
?
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Substrates: no methylation of internal or conventional 5'-terminal guanosine residues
Products: -
Products: -
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additional information
?
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Substrates: ATP, XTP, CTP, UTP, and guanosine containing compounds with phospho groups in either position 2' or 3' or in 3'-5'phosphodiester linkage
Products: -
Products: -
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additional information
?
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Substrates: no substrates are poly-(A) terminating in a single phosphate or a diphosphate
Products: -
Products: -
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additional information
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Substrates: methylation of the cap structure is also implicated in polyadenylation-mediated translational activation during Xenopus oocyte maturation
Products: -
Products: -
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additional information
?
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Substrates: methylation of the cap structure is also implicated in polyadenylation-mediated translational activation during Xenopus oocyte maturation
Products: -
Products: -
?
additional information
?
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Substrates: methylation of the cap structure is also implicated in polyadenylation-mediated translational activation during Xenopus oocyte maturation
Products: -
Products: -
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA]
S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA]
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA]
S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA]
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Substrates: -
Products: -
Products: -
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S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA]
S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA]
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA]
S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA]
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Substrates: the enzyme methylates the guanosine cap on the N-7 position to create the cap structure, m7G(5)ppp(5)X
Products: -
Products: -
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S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA]
S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA]
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Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA]
S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA]
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Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
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Substrates: essential and defining step in the eukaryotic mRNA synthesis
Products: -
Products: -
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S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
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Substrates: essential for cell viability
Products: -
Products: -
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S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
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Substrates: associated with mRNA-guanylyltransferase
Products: -
Products: -
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S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
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Substrates: in infected BHK cells
Products: -
Products: -
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
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Substrates: the presence of the small one is required for methylase activity, the large subunit bears functional domains for binding of the small subunit and for methylase activity
Products: -
Products: -
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S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
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Substrates: guanylyltransferase and RNA-triphosphatase are located on the large subunit
Products: -
Products: -
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S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
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Substrates: one of a series of three reactions in capping of vaccinia virus mRNA catalyzed by one enzyme, which is a heterodimer: no methylase activity with one of the subunits alone
Products: -
Products: -
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S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
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Substrates: R may be guanosine or adenosine, involved in synthesis of methylated capped structure at 5'-terminal of viral mRNA
Products: -
Products: -
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
Substrates: R may be guanosine or adenosine, involved in synthesis of methylated capped structure at 5'-terminal of viral mRNA
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme is responsible for guanine-N7 methylation of recapped mRNAs
Products: -
Products: -
-
additional information
?
-
Substrates: methylation of the cap structure is also implicated in polyadenylation-mediated translational activation during Xenopus oocyte maturation
Products: -
Products: -
?
additional information
?
-
Substrates: methylation of the cap structure is also implicated in polyadenylation-mediated translational activation during Xenopus oocyte maturation
Products: -
Products: -
?
additional information
?
-
Substrates: methylation of the cap structure is also implicated in polyadenylation-mediated translational activation during Xenopus oocyte maturation
Products: -
Products: -
?