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S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA]
S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA]
S-adenosyl-L-methionine + dGTP
S-adenosyl-L-homocysteine + m7dGTP
S-adenosyl-L-methionine + G(5')pppA
S-adenosyl-L-homocysteine + m7G(5')pppA
-
-
-
?
S-adenosyl-L-methionine + G(5')pppG
S-adenosyl-L-homocysteine + m7G(5')pppG
and dGTP, best substrates
-
-
?
S-adenosyl-L-methionine + G(5')pppN
S-adenosyl-L-homocysteine + m7G(5')pppG
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
S-adenosyl-L-methionine + GDP
S-adenosyl-L-homocysteine + m7GDP
-
-
-
?
S-adenosyl-L-methionine + Gppp-RNA
S-adenosyl-L-homocysteine + m7Gppp-RNA
-
-
-
-
?
S-adenosyl-L-methionine + GpppA
S-adenosyl-L-homocysteine + m7GpppA
S-adenosyl-L-methionine + GpppA-RNA
S-adenosyl-L-homocysteine + m7GpppA-RNA
-
-
-
-
?
S-adenosyl-L-methionine + GpppAC3
S-adenosyl-L-homocysteine + m7GpppAC3
-
is efficiently methylated at the guanine-N7 position
-
-
?
S-adenosyl-L-methionine + GpppAC4
S-adenosyl-L-homocysteine + 7mGpppAC4
S-adenosyl-L-methionine + GpppG
S-adenosyl-L-homocysteine + m7GpppG
S-adenosyl-L-methionine + GpppGAU-[RNA]
?
-
strong activity
-
-
?
S-adenosyl-L-methionine + GTP
S-adenosyl-L-homocysteine + m7GTP
S-adenosyl-L-methionine + guanylimidodiphosphate
?
-
-
-
-
?
S-adenosyl-L-methionine + guanylylimidodiphosphate
?
-
-
-
?
S-adenosyl-L-methionine + m7G(5')pppG
S-adenosyl-L-homocysteine + ?
-
-
-
?
S-adenosyl-L-methionine + mGpppGAU-[RNA]
?
-
strong activity
-
-
?
S-adenosyl-L-methionine + mGpppGmAmU-[RNA]
?
-
strong activity
-
-
?
S-adenosyl-L-methionine + mGpppGmAU-[RNA]
?
-
strong activity
-
-
?
S-adenosyl-L-methionine + pp(A)n
?
-
-
-
-
?
additional information
?
-
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA]

S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA]
-
-
-
-
?
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA]
S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA]
-
-
-
?
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA]
S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA]
-
the enzyme methylates the guanosine cap on the N-7 position to create the cap structure, m7G(5)ppp(5)X
-
-
?
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA]
S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA]
-
-
-
-
?
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA]
S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA]
-
-
-
-
?
S-adenosyl-L-methionine + dGTP

S-adenosyl-L-homocysteine + m7dGTP
-
-
-
?
S-adenosyl-L-methionine + dGTP
S-adenosyl-L-homocysteine + m7dGTP
and G(5')pppG, best substrates
-
-
?
S-adenosyl-L-methionine + G(5')pppN

S-adenosyl-L-homocysteine + m7G(5')pppG
-
N: G
-
?
S-adenosyl-L-methionine + G(5')pppN
S-adenosyl-L-homocysteine + m7G(5')pppG
-
unmethylated cap analogue, serves as in vitro substrate
-
?
S-adenosyl-L-methionine + G(5')pppN
S-adenosyl-L-homocysteine + m7G(5')pppG
-
N: G
-
?
S-adenosyl-L-methionine + G(5')pppN
S-adenosyl-L-homocysteine + m7G(5')pppG
-
N: G
-
-
?
S-adenosyl-L-methionine + G(5')pppN
S-adenosyl-L-homocysteine + m7G(5')pppG
-
N: A
-
-
?
S-adenosyl-L-methionine + G(5')pppN
S-adenosyl-L-homocysteine + m7G(5')pppG
-
TbCmt1
-
-
?
S-adenosyl-L-methionine + G(5')pppN
S-adenosyl-L-homocysteine + m7G(5')pppG
-
N: A
-
-
?
S-adenosyl-L-methionine + G(5')pppR-RNA

S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
-
R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
-
-
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
-
-
-
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
essential and defining step in the eukaryotic mRNA synthesis
-
-
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
enzyme contains the conserved S-adenosyl-L-methionine-binding motif VLDLGXGXG, structural features for cap guanine specificity
-
-
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
-
-
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
-
-
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
-
-
-
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
mRNA, synthetic polyribonucleotides terminating in a dinucleoside triphosphate
R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
specific for mRNAs with 5'-terminal sequence G(5')pppA- and G(5')pppG-
R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
R may be guanosine or adenosine, methyl acceptors are unmethylated vaccinia virus mRNAs
R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
active also on E. coli tRNA
R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
free G(5')pppG is utilized, G(5')ppG is a poor substrate, while GTP, GDP, and G(5')ppppG are no substrates
R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
essential for cell viability
-
-
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
-
R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
unmethylated mRNA of reovirus and poly A(+) RNA
R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
associated with mRNA-guanylyltransferase
-
-
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
-
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
methylates also undermethylated poly(A)-rich mRNA
R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
methylates also undermethylated poly(A)-rich mRNA
R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
-
-
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
-
R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
in infected BHK cells
-
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
-
-
-
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
-
-
-
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
-
-
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
-
-
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
-
-
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
-
-
-
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
-
R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
-
R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
-
R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
-
R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
-
R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
acceptor substrate specificity, overview
R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
transfers methyl group from S-adenosyl-L-methionine specifically to position 7 of terminal guanosine
R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
transfers methyl group from S-adenosyl-L-methionine specifically to position 7 of terminal guanosine
R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
utilizes GMP, GDP, guanosine, ITP as substrates
R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
mRNA, synthetic polyribonucleotides terminating in a dinucleoside triphosphate
R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
specific for mRNAs with 5'-terminal sequence G(5')pppA- and G(5')pppG-
R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
specific for mRNAs with 5'-terminal sequence G(5')pppA- and G(5')pppG-
R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
R may be guanosine or adenosine, methyl acceptors are unmethylated vaccinia virus mRNAs
-
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
R may be guanosine or adenosine, methyl acceptors are unmethylated vaccinia virus mRNAs
R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
R may be guanosine or adenosine, methyl acceptors are unmethylated vaccinia virus mRNAs
R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
the presence of the small one is required for methylase activity, the large subunit bears functional domains for binding of the small subunit and for methylase activity
-
-
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
guanylyltransferase and RNA-triphosphatase are located on the large subunit
-
-
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
one of a series of three reactions in capping of vaccinia virus mRNA catalyzed by one enzyme, which is a heterodimer: no methylase activity with one of the subunits alone
-
-
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
R may be guanosine or adenosine, involved in synthesis of methylated capped structure at 5'-terminal of viral mRNA
-
-
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
-
-
-
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
transfers methyl group from S-adenosyl-L-methionine specifically to position 7 of terminal guanosine
R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
acceptor substrate specificity, overview
R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
utilizes GMP, GDP, guanosine, ITP as substrates
R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
mRNA, synthetic polyribonucleotides terminating in a dinucleoside triphosphate
R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
specific for mRNAs with 5'-terminal sequence G(5')pppA- and G(5')pppG-
R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
R may be guanosine or adenosine, methyl acceptors are unmethylated vaccinia virus mRNAs
-
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
-
R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
R may be guanosine or adenosine, involved in synthesis of methylated capped structure at 5'-terminal of viral mRNA
-
-
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
R may be guanosine or adenosine, methyl acceptors are unmethylated vaccinia virus mRNAs
R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
-
-
-
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
-
-
-
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
-
-
-
?
S-adenosyl-L-methionine + G(5')pppR-RNA
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
-
?
S-adenosyl-L-methionine + GpppA

S-adenosyl-L-homocysteine + m7GpppA
-
-
-
?
S-adenosyl-L-methionine + GpppA
S-adenosyl-L-homocysteine + m7GpppA
-
-
-
-
?
S-adenosyl-L-methionine + GpppA
S-adenosyl-L-homocysteine + m7GpppA
-
-
-
-
?
S-adenosyl-L-methionine + GpppA
S-adenosyl-L-homocysteine + m7GpppA
-
TbCmt1
-
-
?
S-adenosyl-L-methionine + GpppAC4

S-adenosyl-L-homocysteine + 7mGpppAC4
-
-
-
-
?
S-adenosyl-L-methionine + GpppAC4
S-adenosyl-L-homocysteine + 7mGpppAC4
-
-
-
-
?
S-adenosyl-L-methionine + GpppG

S-adenosyl-L-homocysteine + m7GpppG
-
TbCmt1
-
-
?
S-adenosyl-L-methionine + GpppG
S-adenosyl-L-homocysteine + m7GpppG
-
-
-
-
?
S-adenosyl-L-methionine + GTP

S-adenosyl-L-homocysteine + m7GTP
-
-
-
?
S-adenosyl-L-methionine + GTP
S-adenosyl-L-homocysteine + m7GTP
-
-
-
?
S-adenosyl-L-methionine + GTP
S-adenosyl-L-homocysteine + m7GTP
-
-
-
?
S-adenosyl-L-methionine + GTP
S-adenosyl-L-homocysteine + m7GTP
-
viral, not host enzyme
-
?
S-adenosyl-L-methionine + GTP
S-adenosyl-L-homocysteine + m7GTP
-
-
-
?
S-adenosyl-L-methionine + GTP
S-adenosyl-L-homocysteine + m7GTP
-
-
-
-
?
additional information

?
-
-
enzyme sequentially methylates the guanine N-7 and ribose 2'-O positions of viral RNA cap. The order of two methylations is determined by the preference of 2'-O methylation for substrate m7GpppA-RNA to GpppA-RNA, and the 2'-O methylation is not absolutely dependent on the prior N-7 methylation
-
-
?
additional information
?
-
does not methylate ATP, CTP, UTP, ITP, or m7GTP
-
-
?
additional information
?
-
-
does not methylate ATP, CTP, UTP, ITP, or m7GTP
-
-
?
additional information
?
-
-
no substrates are poly-(A) terminating in a single phosphate or a diphosphate
-
-
?
additional information
?
-
-
GDP, GTP, G(5')ppppG
-
-
?
additional information
?
-
-
the enzyme is responsible for guanine-N7 methylation of recapped mRNAs
-
-
-
additional information
?
-
-
no activity with 7mGpppA
-
-
-
additional information
?
-
no substrates: ATP, CTP, UTP, dATP, dCTP, dUTP or cap analog m7GpppA
-
-
?
additional information
?
-
-
no substrates: ATP, CTP, UTP, dATP, dCTP, dUTP or cap analog m7GpppA
-
-
?
additional information
?
-
-
no activity with 7mGpppA
-
-
-
additional information
?
-
-
the protein reveals cap-independent methyltransferase activities targeting internal adenosine residues. Besides this, the enzyme domain also methylates the N7 position of the cap guanosine. The enzyme preferentially methylates adenosine residues and is not active on poly G, C and U RNAs
-
-
-
additional information
?
-
-
ATP, XTP, CTP, UTP, and guanosine containing compounds with phospho groups in either position 2' or 3' or in 3'-5'phosphodiester linkage
-
-
?
additional information
?
-
-
isolated methyltransferase domain shows a lower but kinetically equivalent activity than the complete enzyme, which is highly enhanced by association with the D12 subunit
-
-
?
additional information
?
-
-
isolated methyltransferase domain shows a lower but kinetically equivalent activity than the complete enzyme, which is highly enhanced by association with the D12 subunit
-
-
?
additional information
?
-
-
no substrates are poly-(A) terminating in a single phosphate or a diphosphate
-
-
?
additional information
?
-
-
no methylation of internal or conventional 5'-terminal guanosine residues
-
-
?
additional information
?
-
-
ATP, XTP, CTP, UTP, and guanosine containing compounds with phospho groups in either position 2' or 3' or in 3'-5'phosphodiester linkage
-
-
?
additional information
?
-
-
no substrates are poly-(A) terminating in a single phosphate or a diphosphate
-
-
?
additional information
?
-
methylation of the cap structure is also implicated in polyadenylation-mediated translational activation during Xenopus oocyte maturation
-
-
?
additional information
?
-
methylation of the cap structure is also implicated in polyadenylation-mediated translational activation during Xenopus oocyte maturation
-
-
?
additional information
?
-
methylation of the cap structure is also implicated in polyadenylation-mediated translational activation during Xenopus oocyte maturation
-
-
?
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(2-[4-[(4-chlorophenyl)(phenyl)methyl]piperazin-1-yl]ethoxy)acetic acid
-
-
(2R,6S)-2,6-dimethyl-4-[2-methyl-3-[4-(2-methylbutan-2-yl)phenyl]propyl]morpholine
-
-
1,8-dihydroxyanthracen-9(10H)-one
1-(4-tert-butylphenyl)-N-methyl-N-[(naphthalen-1-yl)methyl]methanamine
1-(diphenylmethyl)-4-methylpiperazine hydrochloride (1:1)
-
-
1-(diphenylmethyl)-4-[(2E)-3-phenylprop-2-en-1-yl]piperazine
1-[(4-chlorophenyl)(phenyl)methyl]-4-methylpiperazine
-
-
1-[(4-chlorophenyl)(phenyl)methyl]-4-[(3-methylphenyl)methyl]piperazine
1-[bis(4-fluorophenyl)methyl]-4-[(2,3,4-trimethoxyphenyl)methyl]piperazine
1-[bis(4-fluorophenyl)methyl]-4-[(2Z)-3-phenylprop-2-en-1-yl]piperazine
2,3,7,8-tetrahydroxy[1]benzopyrano[5,4,3-cde][1]benzopyran-5,10-dione
-
-
2-(2-[4-[(4-chlorophenyl)(phenyl)methyl]piperazin-1-yl]ethoxy)ethan-1-ol
-
-
3,3',4',5,5',7-hexahaxdroxyflavone
3,3',4',5,7-pentahydroxyflavone
3,4',5,7-tetrahydroxyflavone
3,8-diamino-5-[3-[diethyl(methyl)azaniumyl]propyl]-6-phenylphenanthridin-5-ium
3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate
-
i.e. CHAPS, strong inhibition
5',N8-Adenosyl-alpha,beta-diaminobutyric acid
-
moderate
amino acid modified structure analogue of adenosyl-L-homocysteine
-
i.e. A9145C, strong
-
aza-S-adenosyl-L-methionine
-
bis[6-(dimethylamino)-2-[(E)-2-(2,5-dimethyl-1-phenyl-1H-pyrrol-3-yl)ethenyl]-1-methylquinolin-1-ium] 4-[(3-carboxy-2-oxidonaphthalen-1-yl)methyl]-3-hydroxynaphthalene-2-carboxylate
carbocyclic aza-S-adenosyl-L-methionine
-
Cd2+
about 17 % residual activity at 5 mM
Co2+
about 20 % residual activity at 5 mM
cycloheximide
-
inhibits protein synthesis in infected BHK cells
deoxycholate
-
inactivation
Digitonin
-
strong inhibition
EDTA
12% residual activity at 5 mM
GpppG
-
inhibits binding of the enzyme to RNA
N,N-bis-(3-D-gluconamidopropyl)-deoxycholamide
-
strong inhibition
N-(2-[[(4-methoxyphenyl)methyl](pyrimidin-2-yl)amino]ethyl)-N,N-dimethylhexadecan-1-aminium
n-octyl-beta-D-gluconpyranoside
-
i.e. octylglucoside, strong inhibition
N-tetradecyl-N,N-dimethyl-3-ammonio-1-propane sulfonate
-
i.e. zwittergent 3-14, strong inhibition
Ni2+
about 25 % residual activity at 5 mM
Oligoadenylic acid mono- and triphosphates
-
S-(2-azaadenosyl)-L-homocysteine
-
moderate
S-(3-Aminoadenosyl)-L-homocysteine
-
weak
S-(3-deazaadenosyl)-L-homocysteine
S-(8-azaadenosyl)-L-homocysteine
S-(N6-Dimethyl-3-deazaadenosyl)-L-homocysteine
-
weak
S-(N6-Methyladenosyl)-L-homocysteine
S-Adenosyl-D-homocysteine
S-adenosyl-homocysteine
-
-
S-adenosyl-L-homocysteine
S-Adenosyl-L-homocysteine structural analogues
S-Adenosyl-L-homocysteine sulfone
S-adenosyl-L-homocysteine sulfoxide
S-Aristeromycinyl-L-homocysteine
S-Inosyl-L-homocysteine
-
weak
S-Tubercidinyl-L-homocysteine
Thesit
-
strong inhibition
-
Triton X-100
-
inactivation, reversible by addition of lipids: cardiolipin, phosphatidylglycerol, and especially phosphatidylserine
1,8-dihydroxyanthracen-9(10H)-one

-
-
1,8-dihydroxyanthracen-9(10H)-one
-
-
1-(4-tert-butylphenyl)-N-methyl-N-[(naphthalen-1-yl)methyl]methanamine

-
-
1-(4-tert-butylphenyl)-N-methyl-N-[(naphthalen-1-yl)methyl]methanamine
-
-
1-(diphenylmethyl)-4-[(2E)-3-phenylprop-2-en-1-yl]piperazine

-
-
1-(diphenylmethyl)-4-[(2E)-3-phenylprop-2-en-1-yl]piperazine
-
-
1-[(4-chlorophenyl)(phenyl)methyl]-4-[(3-methylphenyl)methyl]piperazine

-
-
1-[(4-chlorophenyl)(phenyl)methyl]-4-[(3-methylphenyl)methyl]piperazine
-
-
1-[bis(4-fluorophenyl)methyl]-4-[(2,3,4-trimethoxyphenyl)methyl]piperazine

-
-
1-[bis(4-fluorophenyl)methyl]-4-[(2,3,4-trimethoxyphenyl)methyl]piperazine
-
-
1-[bis(4-fluorophenyl)methyl]-4-[(2Z)-3-phenylprop-2-en-1-yl]piperazine

-
-
1-[bis(4-fluorophenyl)methyl]-4-[(2Z)-3-phenylprop-2-en-1-yl]piperazine
-
-
3,3',4',5,5',7-hexahaxdroxyflavone

-
-
3,3',4',5,5',7-hexahaxdroxyflavone
-
-
3,3',4',5,7-pentahydroxyflavone

-
-
3,3',4',5,7-pentahydroxyflavone
-
-
3,4',5,7-tetrahydroxyflavone

-
-
3,4',5,7-tetrahydroxyflavone
-
-
3,8-diamino-5-[3-[diethyl(methyl)azaniumyl]propyl]-6-phenylphenanthridin-5-ium

-
-
3,8-diamino-5-[3-[diethyl(methyl)azaniumyl]propyl]-6-phenylphenanthridin-5-ium
-
-
aurintricarboxylic acid

-
aurintricarboxylic acid
-
strong inhibition at 0.025 mM
aurintricarboxylic acid
-
strong inhibition at 0.025 mM
bis[6-(dimethylamino)-2-[(E)-2-(2,5-dimethyl-1-phenyl-1H-pyrrol-3-yl)ethenyl]-1-methylquinolin-1-ium] 4-[(3-carboxy-2-oxidonaphthalen-1-yl)methyl]-3-hydroxynaphthalene-2-carboxylate

-
-
bis[6-(dimethylamino)-2-[(E)-2-(2,5-dimethyl-1-phenyl-1H-pyrrol-3-yl)ethenyl]-1-methylquinolin-1-ium] 4-[(3-carboxy-2-oxidonaphthalen-1-yl)methyl]-3-hydroxynaphthalene-2-carboxylate
-
-
Ca2+

about 10 % residual activity at 5 mM
Cu2+

about 18 % residual activity at 5 mM
Mg2+

-
strong
Mn2+

-
strong
N-(2-[[(4-methoxyphenyl)methyl](pyrimidin-2-yl)amino]ethyl)-N,N-dimethylhexadecan-1-aminium

-
-
N-(2-[[(4-methoxyphenyl)methyl](pyrimidin-2-yl)amino]ethyl)-N,N-dimethylhexadecan-1-aminium
-
-
Oligoadenylic acid mono- and triphosphates

-
2'-5'-linked, with varying numbers of phosphate groups, methylated in the 3'-terminal hydroxy group or all three 3'-hydroxy groups, different types of inhibition of viral and L-cell enzyme
-
Oligoadenylic acid mono- and triphosphates
-
2'-5'-linked, with varying numbers of phosphate groups, methylated in the 3'-terminal hydroxy group or all three 3'-hydroxy groups, different types of inhibition of viral and L-cell enzyme
-
S-(3-deazaadenosyl)-L-homocysteine

-
strong
S-(3-deazaadenosyl)-L-homocysteine
-
strong
S-(3-deazaadenosyl)-L-homocysteine
-
-
S-(8-azaadenosyl)-L-homocysteine

-
moderate
S-(8-azaadenosyl)-L-homocysteine
-
weak
S-(N6-Methyladenosyl)-L-homocysteine

-
moderate
S-(N6-Methyladenosyl)-L-homocysteine
-
-
S-Adenosyl-D-homocysteine

-
moderate
S-Adenosyl-D-homocysteine
-
weak
S-Adenosyl-D-homocysteine
-
-
S-adenosyl-L-cysteine

-
moderate
S-adenosyl-L-cysteine
-
-
S-adenosyl-L-homocysteine

-
product inhibition, competitive
S-adenosyl-L-homocysteine
inhibits methylation of GTP in the presence of 0.005 mM S-adenosyl-L-methionine
S-adenosyl-L-homocysteine
-
product inhibition, competitive
S-adenosyl-L-homocysteine
-
inhibits in a concentration-dependent fashion in the presence of 0.025 mM [3H-CH3]S-adenosyl-L-methionine
S-adenosyl-L-homocysteine
-
product inhibition, competitive
S-adenosyl-L-homocysteine
-
-
S-adenosyl-L-homocysteine
-
product inhibition, competitive
S-adenosyl-L-homocysteine
-
-
S-Adenosyl-L-homocysteine structural analogues

-
-
S-Adenosyl-L-homocysteine structural analogues
-
-
S-Adenosyl-L-homocysteine sulfone

-
strong
S-Adenosyl-L-homocysteine sulfone
-
weak
S-Adenosyl-L-homocysteine sulfone
-
-
S-adenosyl-L-homocysteine sulfoxide

-
strong
S-adenosyl-L-homocysteine sulfoxide
-
weak
S-adenosyl-L-homocysteine sulfoxide
-
-
S-Aristeromycinyl-L-homocysteine

-
-
S-Aristeromycinyl-L-homocysteine
-
-
S-Tubercidinyl-L-homocysteine

-
strong
S-Tubercidinyl-L-homocysteine
-
weak
S-Tubercidinyl-L-homocysteine
-
-
sinefungin

-
sinefungin
inhibits Ecm1 with modest potency
sinefungin
-
98.2% inhibition at 0.05 mM
sinefungin
-
extremely potent inhibitor, binds 900fold more avidly than S-adenosylhomocysteine or S-adenosylmethionine, sensitivity to growth inhibition is diminished when Abd1 is overexpressed
sinefungin
IC50 value 0.00169 microM in yeast cell-based assay
sinefungin
-
98.2% inhibition at 0.05 mM
sinefungin
-
i.e. A9145, strong
Zn2+

about 30 % residual activity at 5 mM
additional information

-
no inhibition by S-uridinyl-L-homocysteine, S-cytidinyl-L-homocysteine; S-guanosyl-L-homocysteine
-
additional information
-
detergents prevent the association of S-adenosyl-L-methionine with the enzyme
-
additional information
-
nilutamide and PPDNS do not display efficient inhibition at 0.025 and 0.125 mM
-
additional information
-
2'-AMP, 3'-AMP
-
additional information
-
inhibition by a natural low-molecular-weight inhibitor in the crude extract, removed during purification
-
additional information
-
no inhibition by S-uridinyl-L-homocysteine, S-cytidinyl-L-homocysteine
-
additional information
-
2'-AMP, 3'-AMP
-
additional information
-
nilutamide and PPDNS do not display efficient inhibition at 0.025 and 0.125 mM
-
additional information
-
GTP and GpppA do not inhibit N7 MTase activity
-
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0.1022
(2-[4-[(4-chlorophenyl)(phenyl)methyl]piperazin-1-yl]ethoxy)acetic acid
Homo sapiens
-
at pH 8.0 and 30°C
0.0184
(2R,6S)-2,6-dimethyl-4-[2-methyl-3-[4-(2-methylbutan-2-yl)phenyl]propyl]morpholine
Severe acute respiratory syndrome-related coronavirus
-
at pH 8.0 and 30°C
0.00005 - 0.002
1,8-dihydroxyanthracen-9(10H)-one
0.00128 - 0.011
1-(4-tert-butylphenyl)-N-methyl-N-[(naphthalen-1-yl)methyl]methanamine
0.159
1-(diphenylmethyl)-4-methylpiperazine hydrochloride (1:1)
Homo sapiens
-
at pH 8.0 and 30°C
0.0091 - 0.0168
1-(diphenylmethyl)-4-[(2E)-3-phenylprop-2-en-1-yl]piperazine
0.2268
1-[(4-chlorophenyl)(phenyl)methyl]-4-methylpiperazine
Homo sapiens
-
at pH 8.0 and 30°C
0.0062 - 0.0088
1-[(4-chlorophenyl)(phenyl)methyl]-4-[(3-methylphenyl)methyl]piperazine
0.0135 - 0.0137
1-[bis(4-fluorophenyl)methyl]-4-[(2,3,4-trimethoxyphenyl)methyl]piperazine
0.0073 - 0.0143
1-[bis(4-fluorophenyl)methyl]-4-[(2Z)-3-phenylprop-2-en-1-yl]piperazine
0.000019
2,3,7,8-tetrahydroxy[1]benzopyrano[5,4,3-cde][1]benzopyran-5,10-dione
Severe acute respiratory syndrome-related coronavirus
-
at pH 8.0 and 30°C
0.03652
2-(2-[4-[(4-chlorophenyl)(phenyl)methyl]piperazin-1-yl]ethoxy)ethan-1-ol
Homo sapiens
-
at pH 8.0 and 30°C
0.00032 - 0.00074
3,3',4',5,5',7-hexahaxdroxyflavone
0.0004 - 0.0013
3,3',4',5,7-pentahydroxyflavone
0.01 - 0.0237
3,4',5,7-tetrahydroxyflavone
0.0055 - 0.031
3,8-diamino-5-[3-[diethyl(methyl)azaniumyl]propyl]-6-phenylphenanthridin-5-ium
0.0023 - 0.00572
aurintricarboxylic acid
0.1
aza-S-adenosyl-L-methionine
Encephalitozoon cuniculi
-
0.0045 - 0.0051
bis[6-(dimethylamino)-2-[(E)-2-(2,5-dimethyl-1-phenyl-1H-pyrrol-3-yl)ethenyl]-1-methylquinolin-1-ium] 4-[(3-carboxy-2-oxidonaphthalen-1-yl)methyl]-3-hydroxynaphthalene-2-carboxylate
0.035
carbocyclic aza-S-adenosyl-L-methionine
Encephalitozoon cuniculi
-
0.001 - 0.0015
N-(2-[[(4-methoxyphenyl)methyl](pyrimidin-2-yl)amino]ethyl)-N,N-dimethylhexadecan-1-aminium
1.18
ribavirin
Chikungunya virus
at pH 7.3 and 25°C
0.00177
S-adenosyl-homocysteine
Dengue virus
-
pH 7.5, 20°C
0.004 - 0.021
S-adenosyl-L-homocysteine
0.000021 - 0.00269
sinefungin
0.00005
1,8-dihydroxyanthracen-9(10H)-one

Homo sapiens
-
at pH 8.0 and 30°C
0.002
1,8-dihydroxyanthracen-9(10H)-one
Severe acute respiratory syndrome-related coronavirus
-
at pH 8.0 and 30°C
0.00128
1-(4-tert-butylphenyl)-N-methyl-N-[(naphthalen-1-yl)methyl]methanamine

Homo sapiens
-
at pH 8.0 and 30°C
0.011
1-(4-tert-butylphenyl)-N-methyl-N-[(naphthalen-1-yl)methyl]methanamine
Severe acute respiratory syndrome-related coronavirus
-
at pH 8.0 and 30°C
0.0091
1-(diphenylmethyl)-4-[(2E)-3-phenylprop-2-en-1-yl]piperazine

Severe acute respiratory syndrome-related coronavirus
-
at pH 8.0 and 30°C
0.0168
1-(diphenylmethyl)-4-[(2E)-3-phenylprop-2-en-1-yl]piperazine
Homo sapiens
-
at pH 8.0 and 30°C
0.0062
1-[(4-chlorophenyl)(phenyl)methyl]-4-[(3-methylphenyl)methyl]piperazine

Severe acute respiratory syndrome-related coronavirus
-
at pH 8.0 and 30°C
0.0088
1-[(4-chlorophenyl)(phenyl)methyl]-4-[(3-methylphenyl)methyl]piperazine
Homo sapiens
-
at pH 8.0 and 30°C
0.0135
1-[bis(4-fluorophenyl)methyl]-4-[(2,3,4-trimethoxyphenyl)methyl]piperazine

Severe acute respiratory syndrome-related coronavirus
-
at pH 8.0 and 30°C
0.0137
1-[bis(4-fluorophenyl)methyl]-4-[(2,3,4-trimethoxyphenyl)methyl]piperazine
Homo sapiens
-
at pH 8.0 and 30°C
0.0073
1-[bis(4-fluorophenyl)methyl]-4-[(2Z)-3-phenylprop-2-en-1-yl]piperazine

Severe acute respiratory syndrome-related coronavirus
-
at pH 8.0 and 30°C
0.0143
1-[bis(4-fluorophenyl)methyl]-4-[(2Z)-3-phenylprop-2-en-1-yl]piperazine
Homo sapiens
-
at pH 8.0 and 30°C
0.00032
3,3',4',5,5',7-hexahaxdroxyflavone

Homo sapiens
-
at pH 8.0 and 30°C
0.00074
3,3',4',5,5',7-hexahaxdroxyflavone
Severe acute respiratory syndrome-related coronavirus
-
at pH 8.0 and 30°C
0.0004
3,3',4',5,7-pentahydroxyflavone

Homo sapiens
-
at pH 8.0 and 30°C
0.0013
3,3',4',5,7-pentahydroxyflavone
Severe acute respiratory syndrome-related coronavirus
-
at pH 8.0 and 30°C
0.01
3,4',5,7-tetrahydroxyflavone

Homo sapiens
-
at pH 8.0 and 30°C
0.0237
3,4',5,7-tetrahydroxyflavone
Severe acute respiratory syndrome-related coronavirus
-
at pH 8.0 and 30°C
0.0055
3,8-diamino-5-[3-[diethyl(methyl)azaniumyl]propyl]-6-phenylphenanthridin-5-ium

Homo sapiens
-
at pH 8.0 and 30°C
0.031
3,8-diamino-5-[3-[diethyl(methyl)azaniumyl]propyl]-6-phenylphenanthridin-5-ium
Severe acute respiratory syndrome-related coronavirus
-
at pH 8.0 and 30°C
0.0023
aurintricarboxylic acid

Dengue virus
-
in 50 mM Tris, pH 7.0, 50 mM NaCl, 2 mM dithiothreitol, at 22°C
0.0042
aurintricarboxylic acid
Wesselsbron virus
-
in 50 mM Tris, pH 7.0, 50 mM NaCl, 2 mM dithiothreitol, at 22°C
0.00572
aurintricarboxylic acid
Chikungunya virus
at pH 7.3 and 25°C
0.0045
bis[6-(dimethylamino)-2-[(E)-2-(2,5-dimethyl-1-phenyl-1H-pyrrol-3-yl)ethenyl]-1-methylquinolin-1-ium] 4-[(3-carboxy-2-oxidonaphthalen-1-yl)methyl]-3-hydroxynaphthalene-2-carboxylate

Severe acute respiratory syndrome-related coronavirus
-
at pH 8.0 and 30°C
0.0051
bis[6-(dimethylamino)-2-[(E)-2-(2,5-dimethyl-1-phenyl-1H-pyrrol-3-yl)ethenyl]-1-methylquinolin-1-ium] 4-[(3-carboxy-2-oxidonaphthalen-1-yl)methyl]-3-hydroxynaphthalene-2-carboxylate
Homo sapiens
-
at pH 8.0 and 30°C
0.001
N-(2-[[(4-methoxyphenyl)methyl](pyrimidin-2-yl)amino]ethyl)-N,N-dimethylhexadecan-1-aminium

Homo sapiens
-
at pH 8.0 and 30°C
0.0015
N-(2-[[(4-methoxyphenyl)methyl](pyrimidin-2-yl)amino]ethyl)-N,N-dimethylhexadecan-1-aminium
Severe acute respiratory syndrome-related coronavirus
-
at pH 8.0 and 30°C
0.004
S-adenosyl-L-homocysteine

Encephalitozoon cuniculi
-
0.021
S-adenosyl-L-homocysteine
Saccharomyces cerevisiae
-
-
0.000021
sinefungin

Saccharomyces cerevisiae
-
-
0.00003
sinefungin
Dengue virus
-
pH 7.5, 20°C
0.000112
sinefungin
Severe acute respiratory syndrome-related coronavirus
-
at pH 8.0 and 30°C
0.0015
sinefungin
Encephalitozoon cuniculi
-
0.00269
sinefungin
Chikungunya virus
at pH 7.3 and 25°C
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D146A
-
mutant completely loses the N-7 activity (methylation of N-7 position of guanine) and 2'-O methylation activity. Mutation defective in N-7 methylation is lethal for DNV replication
E217A
-
mutants retains 59% of wild-type N-7 (methylation of N-7 position of guanine) activity
K181A
-
mutants retains 16% of wild-type N-7 (methylation of N-7 position of guanine) activity
K61A
-
mutants retains 34% of wild-type N-7 (methylation of N-7 position of guanine) activity
D122A/S123A
-
site-directed mutagenesis, complementation of the yeast abd1DELTA mutant at 25°C and 30°C, only slightly at 37°C
E225D
growth at all temperatures
E225Q
growth at all temperatures
F141H
grows well at 25 and 30°C but forms pinpoint colonies at 37°C
F141I
grows well at 25 and 30°C but forms pinpoint colonies at 37°C
F141L
grows as well as the wild-type ECM1 strain at all temperatures
F141V
grows well at 25 and 30°C but forms pinpoint colonies at 37°C
F214L
growth at all temperatures, slow growth at 37°C
H144A/Y145F
fully functional
H144A/Y145L
defective in vivo, fails to grow at 37°C, forms microcolonies at 30°C, and grows slowly at 25°C
I95A
-
site-directed mutagenesis, complementation of the yeast abd1DELTA mutant at 25°C, 30°C, and 37°C, no effect on enzyme function
I95A/Y124A
-
site-directed mutagenesis, only slight complementation of the yeast abd1DELTA mutant at 25°C and 30°C, not at 37°C, growth defects
K75Q
slow growth at all temperatures
K75R
growth at all temperatures
L216A
-
site-directed mutagenesis, complementation of the yeast abd1DELTA mutant at 25°C and 30°C, slightly reduced at 37°C, no effect on cell growth
N50A
-
site-directed mutagenesis, complementation of the yeast abd1DELTA mutant at 25°C and 30°C, slightly reduced at 37°C, only modest growth defects
N50A/Y284A
-
site-directed mutagenesis, no complementation of the yeast abd1DELTA mutant, mutation is lethal in vivo
P175A
grows as well as the wild-type ECM1 strain at all temperatures
R47A
-
site-directed mutagenesis, complementation of the yeast abd1DELTA mutant at 25°C and 30°C, slightly reduced at 37°C
R47A/K75A
-
site-directed mutagenesis, no complementation of the yeast abd1DELTA mutant, mutation is lethal in vivo
Y124F
growth at all temperatures, slow growth at 37°C
Y145H
grows as well as the wild-type ECM1 strain at all temperatures
Y145I
intermediate phenotype of slow growth at 25 and 30°C and microcolony formation at 37°C
Y212F
growth at all temperatures
Y284A
-
site-directed mutagenesis, some complementation of the yeast abd1DELTA mutant at 25°C and 30°C, not at 37°C, slow growth phenotype
D330A
-
the mutation does not affect viral replication, sensitivity to mutagen, or inhibition by interferon-beta compared to the wild type
G332A
-
the mutant displays delayed replication kinetics and decreased peak titers relative to the wild type virus. In addition, replication of mutant virus is diminished following treatment of cells with interferon-beta, and mutant genomes are translated less efficiently both in vitro and during viral infection
C416R
mutation abolishes the N7-MTase activity on GTP
D273A
97% of wild-type methylation activity
D331E
70% of wild-type methylation activity
G416R
45% of wild-type methylation activity
K61A
97% of wild-type methylation activity
L419A
complete loss of methylation activity
L419R
15-50% of wild-type activity
N256A
97% of wild-type methylation activity
N334A
89% of wild-type methylation activity
N388A
83% of wild-type methylation activity
D1924A
-
the mutant shows severely reduced activity compared to the wild type enzyme
E1996A
-
the mutant shows severely reduced activity compared to the wild type enzyme
K1813A
-
the mutant shows severely reduced activity compared to the wild type enzyme
K1959A
-
the mutant shows severely reduced activity compared to the wild type enzyme
D144A
-
TbCmt1 mutant shows 5% of wild-type activity
D80A
-
TbCmt1 mutant is unable to support methylation at levels of input protein sufficient to convert all the input substrate to the methylated form by wild-type TbCmt1, specific activity is less than 0.1% of the wild-type activity
E258A
-
TbCmt1 mutant is unable to support methylation at levels of input protein sufficient to convert all the input substrate to the methylated form by wild-type TbCmt1, specific activity is less than 0.1% of the wild-type activity
F171A
-
TbCmt1 mutant displays near-wild-type activity
H174A
-
TbCmt1 mutant displays near-wild-type activity
K83A
-
TbCmt1 mutant is unable to support methylation at levels of input protein sufficient to convert all the input substrate to the methylated form by wild-type TbCmt1, specific activity is less than 0.1% of the wild-type activity
V241A
-
TbCmt1 mutant displays near-wild-type activity
V242A
-
TbCmt1 mutant displays near-wild-type activity
Y175A
-
TbCmt1 mutant shows 3% of wild-type activity
Y246A
-
TbCmt1 mutant shows 2% of wild-type activity
C173A/S174A
-
modest defect, grows well at 19, 30 and 34°C, but very slowly at 37°C
D192A/S193A
-
temperature-sensitive vD12 allele, near-lethality, only grows very slowly at 19°C
D52A/L53A
-
modest defect, grows well at 30°C, but slowly at 34°C and not at 37°C
D545A
-
the mutant has 102% of wild type activity
D598E
-
the mutant enzyme has 1% of wild type activity
D598N
-
the mutant enzyme hasless than 1% of wild type activity
D604E
-
the mutant enzyme has 112% of wild type activity
D604N
-
the mutant enzyme has less than 1% of wild type activity
D620E
-
the mutant enzyme has 59% of wild type activity
D620N
-
the mutant enzyme has 11% of wild type activity
D657A
-
the mutant enzyme has 112% of wild type activity
D676A
-
the mutant enzyme has less than 1% of wild type activity
D676E
-
the mutant enzyme has 40% of wild type activity
D676N
-
the mutant enzyme has less than 1% of wild type activity
E104A/G11A
-
modest defect, grows well at 19, 30 and 34 °C, but slowly at 37°C
E24A-25A
-
temperature-sensitive vD12 allele, does not grow at 37°C
E275A/N276A
-
modest defect, grows well at 19, 30 and 34°C, but slowly at 37°C
E763A
-
the mutant enzyme has 16% of wild type activity
E763D
-
the mutant enzyme has 1% of wild type activity
E763Q
-
the mutant enzyme has 32% of wild type activity
E816A
-
the mutant enzyme has 76% of wild type activity
F176A/K177A
-
lethal at all temperatures
F44A/L45A
-
modest defect, grows well at 19, 30 and 34°C, but slowly at 37°C
F556A
-
the mutant has 1% of wild type activity
F556L
-
the mutant has 3% of wild type activity
F609A
-
the mutant enzyme has 117% of wild type activity
F659A
-
the mutant enzyme has 96% of wild type activity
F679A
-
the mutant enzyme has 5% of wild type activity
F679H
-
the mutant enzyme has 13% of wild type activity
F679I
-
the mutant enzyme has 18% of wild type activity
F679L
-
the mutant enzyme has 55% of wild type activity
F679N
-
the mutant enzyme has 18% of wild type activity
F679V
-
the mutant enzyme has 20% of wild type activity
F814A
-
the mutant enzyme has 99% of wild type activity
F815A
-
the mutant enzyme has 92% of wild type activity
G600A
-
in D1 fragment, amino acids 498-844, 4% activity remaining
H260A/S261A
-
modest defect, grows well at 19, 30 and 34°C, but very slowly at 37°C
H682N
-
the mutant enzyme has 1% of wild type activity
H682Q
-
the mutant enzyme has 4% of wild type activity
I47A/S48A
-
modest defect, grows well at 19, 30 and 34°C, but slowly at 37°C
K111A/R112A
-
modest defect, grows well at 19, 30 and 34°C, but very slowly at 37°C
K156A
-
viable at 19°C and 30°C, but fails to grow at 34°C
K156A/L157A
-
lethal at all temperatures
K223A/D223A
-
modest defect, grows well at 19, 30 and 34°C, but slowly at 37°C
K573A
-
the mutant enzyme has less than 1% of wild type activity
K573Q
-
the mutant enzyme has less than 1% of wild type activity
K573R
-
the mutant enzyme has 1% of wild type activity
K607A
-
the mutant enzyme has101% of wild type activity
K607Q
-
the mutant enzyme has 74% of wild type activity
K607R
-
the mutant enzyme has 13% of wild type activity
L167A
-
viable at 19°C and 30°C, but fails to grow at 34°C
L16A/P17A
-
temperature-sensitive vD12 allele, forms small colonies at 19°C and fails to grow at 30°C or higher
N120A/N121A
-
modest defect, grows well at 19, 30 and 34°C, but slowly at 37°C
N126A/N127A
-
modest defect, grows well at 19, 30 and 34°C, but very slowly at 37°C
N42A-Y43A
-
temperature-sensitive vD12 allele, does not grow at 37°C
N54A/R55A
-
modest defect, grows well at 19 and 30°C, but slowly at 34 and very slowly at 37°C
N550A
-
the mutant has 3% of wild type activity
N550D
-
the mutant has 19% of wild type activity
N550Q
-
the mutant has 10% of wild type activity
N570A
-
the mutant enzyme has 37% of wild type activity
N570D
-
the mutant enzyme has 21% of wild type activity
N570Q
-
the mutant enzyme has 73% of wild type activity
N601A
-
the mutant enzyme has 32% of wild type activity
P158A/T159A
-
temperature-sensitive vD12 allele, only grows at 19°C
P35A/S36A
-
modest defect, grows well at 19, 30 and 34°C, but slowly at 37°C
Q678A
-
the mutant enzyme has 55% of wild type activity
R280A-R281A
-
modest defect, grows well at 19, 30 and 34°C, but slowly at 37°C
R548A
-
the mutant has 59% of wild type activity
R560A
-
the mutant has 18% of wild type activity
R560K
-
the mutant has 84% of wild type activity
R560Q
-
the mutant has 84% of wild type activity
R562A
-
the mutant has 10% of wild type activity
R562K
-
the mutant has 25% of wild type activity
R562Q
-
the mutant has 23% of wild type activity
R632K
-
the mutant enzyme has 72% of wild type activity
R632Q
-
the mutant enzyme has 58% of wild type activity
R655A
-
the mutant has 31% of wild type activity
R794A
-
the mutant has 124% of wild type activity
R808A
-
the mutant has 142% of wild type activity