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Literature summary for 2.1.1.56 extracted from

  • Case, J.B.; Ashbrook, A.W.; Dermody, T.S.; Denison, M.R.
    Mutagenesis of S-adenosyl-L-methionine-binding residues in coronavirus nsp14 N7-methyltransferase demonstrates differing requirements for genome translation and resistance to innate immunity (2016), J. Virol., 90, 7248-7256 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
D330A the mutation does not affect viral replication, sensitivity to mutagen, or inhibition by interferon-beta compared to the wild type Murine hepatitis virus
G332A the mutant displays delayed replication kinetics and decreased peak titers relative to the wild type virus. In addition, replication of mutant virus is diminished following treatment of cells with interferon-beta, and mutant genomes are translated less efficiently both in vitro and during viral infection Murine hepatitis virus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] Murine hepatitis virus
-
S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA]
-
?

Organism

Organism UniProt Comment Textmining
Murine hepatitis virus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA]
-
Murine hepatitis virus S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA]
-
?

Synonyms

Synonyms Comment Organism
N7-methyltransferase
-
Murine hepatitis virus
N7-MTase
-
Murine hepatitis virus
nsp14
-
Murine hepatitis virus