BRENDA - Enzyme Database
show all sequences of 2.1.1.56

Mutational analysis of Encephalitozoon cuniculi mRNA cap (guanine-N7) methyltransferase, structure of the enzyme bound to sinefungin, and evidence that cap methyltransferase is the target of sinefungins antifungal activity

Zheng, S.; Hausmann, S.; Liu, Q.; Ghosh, A.; Schwer, B.; Lima, C.D.; Shuman, S.; J. Biol. Chem. 281, 35904-35913 (2006)

Data extracted from this reference:

Application
Application
Commentary
Organism
analysis
cap methylation is a principal target of the antifungal activity of sinefungin
Encephalitozoon cuniculi
drug development
cap methylation is a principal target of the antifungal activity of sinefungin
Saccharomyces cerevisiae
Cloned(Commentary)
Cloned (Commentary)
Organism
as a His6-Smt3 fusion protein in Escherichia coli BL21(DE3) CodonPlus RIL using a pET-based pSmt3-TOPO vector
Saccharomyces cerevisiae
yeast strain YBS40 transformed with CEN TRP1 plasmids containing the wild-type and mutant alleles of ECM1, the pET16-Ecm1 plasmids introduced into Escherichia coli BL21(DE3)
Encephalitozoon cuniculi
Crystallization (Commentary)
Crystallization (Commentary)
Organism
Ecm1-sinefungin binary complex, by vapor diffusion, to 2.6 A resolution
Encephalitozoon cuniculi
Engineering
Protein Variants
Commentary
Organism
D70A
lethal
Encephalitozoon cuniculi
D70E
grows as well as the wild-type ECM1 strain at all temperatures
Encephalitozoon cuniculi
D70N
lethal
Encephalitozoon cuniculi
D78A
lethal
Encephalitozoon cuniculi
D78E
no growth at 37°C
Encephalitozoon cuniculi
D78N
lethal
Encephalitozoon cuniculi
D94A
lethal
Encephalitozoon cuniculi
D94E
grows as well as the wild-type ECM1 strain at all temperatures
Encephalitozoon cuniculi
D94N
lethal
Encephalitozoon cuniculi
E225A
no growth at 37°C
Encephalitozoon cuniculi
F141A
lethal
Encephalitozoon cuniculi
F141H
grows well at 25 and 30°C but forms pinpoint colonies at 37°C
Encephalitozoon cuniculi
F141I
grows well at 25 and 30°C but forms pinpoint colonies at 37°C
Encephalitozoon cuniculi
F141L
grows as well as the wild-type ECM1 strain at all temperatures
Encephalitozoon cuniculi
F141N
lethal
Encephalitozoon cuniculi
F141V
grows well at 25 and 30°C but forms pinpoint colonies at 37°C
Encephalitozoon cuniculi
F214A
no growth at 37°C
Encephalitozoon cuniculi
H144A
no effect on yeast growth at 25 or 30°C, although it slowly grows at 37°C
Encephalitozoon cuniculi
H144A/Y145F
fully functional
Encephalitozoon cuniculi
H144A/Y145L
defective in vivo, fails to grow at 37°C, forms microcolonies at 30°C, and grows slowly at 25°C
Encephalitozoon cuniculi
K267A
grows as well as the wild-type ECM1 strain at all temperatures and is as active as wild-type Ecm1 in GpppA methylation in vitro
Encephalitozoon cuniculi
K54A
lethal
Encephalitozoon cuniculi
K54Q
lethal
Encephalitozoon cuniculi
K54R
lethal
Encephalitozoon cuniculi
K75A
no growth at 37°C
Encephalitozoon cuniculi
K81A
lethal
Encephalitozoon cuniculi
K81Q
lethal
Encephalitozoon cuniculi
K81R
lethal
Encephalitozoon cuniculi
N51A
no growth at 37°C
Encephalitozoon cuniculi
N51D
lethal
Encephalitozoon cuniculi
P175A
grows as well as the wild-type ECM1 strain at all temperatures
Encephalitozoon cuniculi
R106A
lethal
Encephalitozoon cuniculi
R106K
lethal
Encephalitozoon cuniculi
R106Q
lethal
Encephalitozoon cuniculi
R59A
grows as well as the wild-type ECM1 strain at all temperatures and is as active as wild-type Ecm1 in GpppA methylation in vitro
Encephalitozoon cuniculi
R84A
grows as well as the wild-type ECM1 strain at all temperatures and is as active as wild-type Ecm1 in GpppA methylation in vitro
Encephalitozoon cuniculi
Y124A
no growth at 37°C
Encephalitozoon cuniculi
Y145A
lethal
Encephalitozoon cuniculi
Y145F
grows as well as the wild-type ECM1 strain at all temperatures
Encephalitozoon cuniculi
Y145H
grows as well as the wild-type ECM1 strain at all temperatures
Encephalitozoon cuniculi
Y145I
intermediate phenotype of slow growth at 25 and 30°C and microcolony formation at 37°C
Encephalitozoon cuniculi
Y145L
grows as well as the wild-type ECM1 strain at all temperatures
Encephalitozoon cuniculi
Y145S
lethal
Encephalitozoon cuniculi
Y145V
lethal
Encephalitozoon cuniculi
Y212A
no growth at 37°C
Encephalitozoon cuniculi
Inhibitors
Inhibitors
Commentary
Organism
Structure
S-adenosyl-L-homocysteine
inhibits in a concentration-dependent fashion in the presence of 0.025 mM [3H-CH3]S-adenosyl-L-methionine
Saccharomyces cerevisiae
sinefungin
inhibits Ecm1 with modest potency
Encephalitozoon cuniculi
sinefungin
extremely potent inhibitor, binds 900fold more avidly than S-adenosylhomocysteine or S-adenosylmethionine, sensitivity to growth inhibition is diminished when Abd1 is overexpressed
Saccharomyces cerevisiae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.006
-
S-adenosyl-L-methionine
-
Saccharomyces cerevisiae
0.24
-
GpppA
-
Saccharomyces cerevisiae
Organism
Organism
UniProt
Commentary
Textmining
Encephalitozoon cuniculi
Q8SR66
-
-
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
His6-Smt3-Abd1 by nickel-nitrilotriacetic acid fast flow column chromatography, Abd1 free of the tag by gel filtration, to homogeneity
Saccharomyces cerevisiae
recombinant Ecm1 mutant proteins, by nickel-agarose chromatography
Encephalitozoon cuniculi
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
S-adenosyl-L-methionine + GpppA
-
674701
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + m7GpppA
-
-
-
?
S-adenosyl-L-methionine + GpppA
-
674701
Encephalitozoon cuniculi
S-adenosyl-L-homocysteine + m7GpppA
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
Abd1
-
Saccharomyces cerevisiae
Ecm1
-
Encephalitozoon cuniculi
mRNA cap (guanine-N7) methyltransferase
-
Encephalitozoon cuniculi
mRNA cap (guanine-N7) methyltransferase
-
Saccharomyces cerevisiae
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
25
37
-
Encephalitozoon cuniculi
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0983
-
GpppA
-
Saccharomyces cerevisiae
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.000021
-
-
Saccharomyces cerevisiae
sinefungin
0.021
-
-
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine
Application (protein specific)
Application
Commentary
Organism
analysis
cap methylation is a principal target of the antifungal activity of sinefungin
Encephalitozoon cuniculi
drug development
cap methylation is a principal target of the antifungal activity of sinefungin
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
Commentary
Organism
as a His6-Smt3 fusion protein in Escherichia coli BL21(DE3) CodonPlus RIL using a pET-based pSmt3-TOPO vector
Saccharomyces cerevisiae
yeast strain YBS40 transformed with CEN TRP1 plasmids containing the wild-type and mutant alleles of ECM1, the pET16-Ecm1 plasmids introduced into Escherichia coli BL21(DE3)
Encephalitozoon cuniculi
Crystallization (Commentary) (protein specific)
Crystallization
Organism
Ecm1-sinefungin binary complex, by vapor diffusion, to 2.6 A resolution
Encephalitozoon cuniculi
Engineering (protein specific)
Protein Variants
Commentary
Organism
D70A
lethal
Encephalitozoon cuniculi
D70E
grows as well as the wild-type ECM1 strain at all temperatures
Encephalitozoon cuniculi
D70N
lethal
Encephalitozoon cuniculi
D78A
lethal
Encephalitozoon cuniculi
D78E
no growth at 37°C
Encephalitozoon cuniculi
D78N
lethal
Encephalitozoon cuniculi
D94A
lethal
Encephalitozoon cuniculi
D94E
grows as well as the wild-type ECM1 strain at all temperatures
Encephalitozoon cuniculi
D94N
lethal
Encephalitozoon cuniculi
E225A
no growth at 37°C
Encephalitozoon cuniculi
F141A
lethal
Encephalitozoon cuniculi
F141H
grows well at 25 and 30°C but forms pinpoint colonies at 37°C
Encephalitozoon cuniculi
F141I
grows well at 25 and 30°C but forms pinpoint colonies at 37°C
Encephalitozoon cuniculi
F141L
grows as well as the wild-type ECM1 strain at all temperatures
Encephalitozoon cuniculi
F141N
lethal
Encephalitozoon cuniculi
F141V
grows well at 25 and 30°C but forms pinpoint colonies at 37°C
Encephalitozoon cuniculi
F214A
no growth at 37°C
Encephalitozoon cuniculi
H144A
no effect on yeast growth at 25 or 30°C, although it slowly grows at 37°C
Encephalitozoon cuniculi
H144A/Y145F
fully functional
Encephalitozoon cuniculi
H144A/Y145L
defective in vivo, fails to grow at 37°C, forms microcolonies at 30°C, and grows slowly at 25°C
Encephalitozoon cuniculi
K267A
grows as well as the wild-type ECM1 strain at all temperatures and is as active as wild-type Ecm1 in GpppA methylation in vitro
Encephalitozoon cuniculi
K54A
lethal
Encephalitozoon cuniculi
K54Q
lethal
Encephalitozoon cuniculi
K54R
lethal
Encephalitozoon cuniculi
K75A
no growth at 37°C
Encephalitozoon cuniculi
K81A
lethal
Encephalitozoon cuniculi
K81Q
lethal
Encephalitozoon cuniculi
K81R
lethal
Encephalitozoon cuniculi
N51A
no growth at 37°C
Encephalitozoon cuniculi
N51D
lethal
Encephalitozoon cuniculi
P175A
grows as well as the wild-type ECM1 strain at all temperatures
Encephalitozoon cuniculi
R106A
lethal
Encephalitozoon cuniculi
R106K
lethal
Encephalitozoon cuniculi
R106Q
lethal
Encephalitozoon cuniculi
R59A
grows as well as the wild-type ECM1 strain at all temperatures and is as active as wild-type Ecm1 in GpppA methylation in vitro
Encephalitozoon cuniculi
R84A
grows as well as the wild-type ECM1 strain at all temperatures and is as active as wild-type Ecm1 in GpppA methylation in vitro
Encephalitozoon cuniculi
Y124A
no growth at 37°C
Encephalitozoon cuniculi
Y145A
lethal
Encephalitozoon cuniculi
Y145F
grows as well as the wild-type ECM1 strain at all temperatures
Encephalitozoon cuniculi
Y145H
grows as well as the wild-type ECM1 strain at all temperatures
Encephalitozoon cuniculi
Y145I
intermediate phenotype of slow growth at 25 and 30°C and microcolony formation at 37°C
Encephalitozoon cuniculi
Y145L
grows as well as the wild-type ECM1 strain at all temperatures
Encephalitozoon cuniculi
Y145S
lethal
Encephalitozoon cuniculi
Y145V
lethal
Encephalitozoon cuniculi
Y212A
no growth at 37°C
Encephalitozoon cuniculi
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.000021
-
-
Saccharomyces cerevisiae
sinefungin
0.021
-
-
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
S-adenosyl-L-homocysteine
inhibits in a concentration-dependent fashion in the presence of 0.025 mM [3H-CH3]S-adenosyl-L-methionine
Saccharomyces cerevisiae
sinefungin
inhibits Ecm1 with modest potency
Encephalitozoon cuniculi
sinefungin
extremely potent inhibitor, binds 900fold more avidly than S-adenosylhomocysteine or S-adenosylmethionine, sensitivity to growth inhibition is diminished when Abd1 is overexpressed
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.006
-
S-adenosyl-L-methionine
-
Saccharomyces cerevisiae
0.24
-
GpppA
-
Saccharomyces cerevisiae
Purification (Commentary) (protein specific)
Commentary
Organism
His6-Smt3-Abd1 by nickel-nitrilotriacetic acid fast flow column chromatography, Abd1 free of the tag by gel filtration, to homogeneity
Saccharomyces cerevisiae
recombinant Ecm1 mutant proteins, by nickel-agarose chromatography
Encephalitozoon cuniculi
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
S-adenosyl-L-methionine + GpppA
-
674701
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + m7GpppA
-
-
-
?
S-adenosyl-L-methionine + GpppA
-
674701
Encephalitozoon cuniculi
S-adenosyl-L-homocysteine + m7GpppA
-
-
-
?
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
25
37
-
Encephalitozoon cuniculi
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0983
-
GpppA
-
Saccharomyces cerevisiae
Other publictions for EC 2.1.1.56
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733122
Sun
Yeast-based assays for the hig ...
SARS coronavirus
Antiviral Res.
104
156-164
2014
-
1
-
-
-
-
1
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
734586
Chen
Structure-function analysis of ...
SARS coronavirus
J. Virol.
87
6296-6305
2013
-
-
1
1
27
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
27
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
735335
Jin
Characterization of the guanin ...
SARS coronavirus
Virus Res.
176
45-52
2013
-
-
-
-
18
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
18
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
735240
Thillier
Synthesis of 5' cap-0 and cap- ...
Homo sapiens
RNA
18
856-868
2012
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
704920
Selisko
Biochemical characterization o ...
Dengue virus
J. Gen. Virol.
91
112-121
2010
-
-
-
-
-
-
1
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
721099
Chung
Higher catalytic efficiency of ...
Dengue virus
Virology
402
52-60
2010
-
-
-
-
-
-
2
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
2
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
721100
Dong
Biochemical and genetic charac ...
Dengue virus
Virology
405
568-578
2010
-
-
1
-
4
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
721101
Zhang
Identification of aromatic ami ...
vesicular stomatitis virus
Virology
408
241-252
2010
-
-
-
-
16
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
16
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
701751
Milani
Flaviviral methyltransferase/R ...
Dengue virus, Wesselsbron virus
Antiviral Res.
83
28-34
2009
-
-
-
-
-
-
4
-
-
-
-
-
-
4
-
-
2
-
-
-
-
-
2
-
6
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
2
4
-
-
-
-
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
706020
Cowling
Enhanced mRNA cap methylation ...
Homo sapiens
Oncogene
29
930-936
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
706535
Chen
Functional screen reveals SARS ...
SARS coronavirus
Proc. Natl. Acad. Sci. USA
106
3484-3489
2009
-
-
1
-
4
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
690028
Zheng
Mutational analysis of vaccini ...
Vaccinia virus
RNA
14
2297-2304
2008
-
-
-
-
20
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
20
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
705423
Dong
West Nile virus methyltransfer ...
West Nile virus
J. Virol.
82
4295-4307
2008
-
-
-
-
20
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
20
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
706721
Zheng
Structure-function analysis of ...
Vaccinia virus
RNA
14
696-705
2008
-
-
1
-
49
-
-
16
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
1
2
-
-
-
14
-
-
-
-
-
-
-
-
-
1
-
-
49
-
-
-
-
16
-
-
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
14
-
-
-
-
-
-
-
-
-
-
676231
Peyrane
High-yield production of short ...
Homo sapiens
Nucleic Acids Res.
35
e26
2007
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
674701
Zheng
Mutational analysis of Encepha ...
Encephalitozoon cuniculi, Saccharomyces cerevisiae
J. Biol. Chem.
281
35904-35913
2006
-
2
2
1
45
-
3
2
-
-
-
-
-
4
-
-
2
-
-
-
-
-
2
-
4
-
1
-
1
-
-
-
-
-
-
2
-
2
2
-
1
45
-
2
3
-
2
-
-
-
-
-
-
-
2
-
-
-
-
2
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
677098
Hall
Characterization of a Trypanos ...
Trypanosoma brucei
RNA
12
488-497
2006
-
1
1
-
10
-
-
-
-
-
1
-
-
4
-
-
1
-
-
-
-
-
3
1
3
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
10
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
677246
Schwer
Genetic analysis of poxvirus m ...
Vaccinia virus
Virology
352
145-156
2006
-
1
1
-
28
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
28
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
659939
Shafer
Human mRNA cap methyltransfera ...
Homo sapiens
Mol. Cell. Biol.
25
2644-2649
2005
-
-
1
-
1
-
-
-
2
-
-
1
-
3
-
-
-
-
-
1
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
674439
Hausmann
Encephalitozoon cuniculi mRNA ...
Encephalitozoon cuniculi
J. Biol. Chem.
280
20404-20412
2005
-
-
1
1
38
-
4
3
-
-
1
-
-
2
-
-
1
-
-
-
-
1
5
1
2
-
-
-
-
1
1
-
1
-
-
4
-
-
1
1
1
38
-
4
4
-
3
-
-
1
-
-
-
-
1
-
-
-
1
5
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
659921
Fabrega
Structure and mechanism of mRN ...
Encephalitozoon cuniculi
Mol. Cell
13
77-89
2004
-
-
1
1
18
-
-
-
-
-
-
1
-
1
-
-
1
1
-
-
-
-
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
18
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485473
Li
Characterization of the Adomet ...
bamboo mosaic virus
J. Virol.
75
782-788
2001
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
485470
Yokoska
Cloning and characterization o ...
Xenopus laevis
Biochem. Biophys. Res. Commun.
268
617-624
2000
-
-
1
-
-
-
-
-
-
-
3
3
-
3
-
-
-
1
-
1
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
-
-
2
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485471
Wen
Cap methyltransferase selectiv ...
Homo sapiens
Genes Dev.
14
2944-2949
2000
1
-
1
-
-
-
1
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485466
Ahola
Semliki forest virus mRNA capp ...
Semliki forest virus
EMBO J.
18
3164-3172
1999
-
-
1
-
-
-
-
-
-
-
-
-
-
6
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485465
Ramadevi
Capping and methylation of mRN ...
bluetongue virus
Proc. Natl. Acad. Sci. USA
95
13537-13542
1998
-
-
1
-
1
1
9
-
2
-
-
-
-
1
-
-
1
1
-
-
-
-
3
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
1
-
9
-
-
2
-
-
-
-
-
-
1
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
485469
Tsukamoto
Cloning and characterization o ...
Homo sapiens, Saccharomyces cerevisiae
Biochem. Biophys. Res. Commun.
251
27-34
1998
-
-
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
2
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
485472
Mao
Vaccinia virus mRNA (guanine-7 ...
Vaccinia virus
Biochemistry
35
6900-6910
1996
-
-
1
-
6
-
-
-
-
-
-
-
-
4
-
-
-
1
-
-
-
-
1
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
485467
Higman
The mRNA (guanine-7-)methyltra ...
Vaccinia virus
J. Biol. Chem.
269
14974-14981
1994
-
-
1
-
-
-
1
5
-
-
2
-
-
1
-
-
1
1
-
-
1
-
4
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
5
-
-
2
-
-
-
-
1
-
-
1
-
4
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
485468
Mao
Intrinsic RNA (guanine-7) meth ...
Vaccinia virus
J. Biol. Chem.
269
24472-24479
1994
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
1
1
-
-
1
-
2
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
485455
Cong
Methyltransferase and subunit ...
Vaccinia virus
J. Biol. Chem.
267
16424-16429
1992
-
-
1
-
1
-
10
-
1
-
3
2
-
1
-
-
-
1
-
-
-
-
3
2
-
1
-
-
-
-
-
-
-
12
-
-
-
-
1
-
-
1
-
-
10
12
-
1
-
3
2
-
-
-
-
-
-
-
-
3
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
485456
Higman
The vaccinia virus mRNA (guani ...
Vaccinia virus
J. Biol. Chem.
267
16430-16437
1992
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
1
1
-
-
-
-
3
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
485463
Guo
Interaction and mutual stabili ...
Vaccinia virus
Proc. Natl. Acad. Sci. USA
87
4023-4027
1990
-
-
1
-
-
1
-
-
-
-
3
3
-
1
-
-
1
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
3
3
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485451
Cross
Identification of a unique gua ...
Semliki forest virus
Virology
130
452-463
1983
-
-
-
-
-
1
1
-
1
-
-
1
-
3
-
-
-
-
-
1
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
1
-
-
-
-
-
1
-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
485462
Locht
Partial purification and chara ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae VY1160
Eur. J. Biochem.
134
117-121
1983
-
-
-
-
-
-
5
1
-
4
1
-
-
2
-
-
1
-
-
1
1
1
2
1
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
1
-
4
1
-
-
-
-
1
-
1
1
1
2
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
485450
Pugh
Effects of S-adenosylhomocyste ...
Vaccinia virus
Biochemistry
21
1535-1541
1982
-
-
-
-
-
-
19
-
1
-
-
2
-
1
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
19
-
-
1
-
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
485461
Goswami
2-5-Linked oligo(adenylic acid ...
Mus musculus, Vaccinia virus
J. Biol. Chem.
257
6867-6870
1982
-
-
-
-
-
-
4
-
1
-
-
-
-
2
-
-
2
-
-
1
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
2
-
1
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
485460
Germershausen
5 Cap methylation of homologou ...
Neurospora crassa
Biochem. Biophys. Res. Commun.
82
871-878
1978
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
2
-
1
4
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
2
-
1
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
485464
Monroy
Purification of mRNA guanylylt ...
Vaccinia virus, Vaccinia virus WR
J. Biol. Chem.
253
4481-4489
1978
-
-
-
-
-
1
-
-
1
-
1
-
-
11
-
-
1
-
-
-
-
1
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
1
-
-
-
1
2
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
485459
Pugh
Inhibition of Newcastle diseas ...
Avian avulavirus 1
Biochemistry
16
3928-3932
1977
-
-
-
-
-
-
10
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485457
Ensinger
Modification of the 5 terminus ...
Homo sapiens
J. Biol. Chem.
251
5283-5291
1976
-
-
-
-
-
-
-
-
2
3
1
-
-
1
-
-
1
-
-
1
1
2
8
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
3
1
-
-
-
-
1
-
1
1
2
8
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
485458
Martin
mRNA guanylyltransferase and m ...
Vaccinia virus, Vaccinia virus WR
J. Biol. Chem.
251
7313-7321
1976
-
-
-
-
-
-
-
-
1
1
-
-
-
11
-
-
1
-
-
-
-
-
13
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
1
-
-
-
-
13
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
485452
Martin
Purification of mRNA guanylylt ...
Vaccinia virus, Vaccinia virus WR
J. Biol. Chem.
250
9322-9329
1975
-
-
-
-
-
-
2
-
1
-
2
2
-
11
-
-
1
-
-
-
-
1
4
1
-
1
-
-
-
1
-
-
-
3
-
-
-
-
-
-
-
-
-
-
2
3
-
1
-
2
2
-
-
-
1
-
-
-
1
4
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
485453
Martin
Modification of RNA by mRNA gu ...
Vaccinia virus
J. Biol. Chem.
250
9330-9335
1975
-
-
-
-
-
-
6
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
6
-
-
1
1
-
-
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
485454
Ensinger
Modification of the 5-terminus ...
Vaccinia virus
Proc. Natl. Acad. Sci. USA
72
2525-2529
1975
-
-
-
-
-
-
2
-
1
2
-
-
-
1
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
2
-
-
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-