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Literature summary for 2.1.1.56 extracted from

  • Martin, B.; Coutard, B.; Guez, T.; Paesen, G.C.; Canard, B.; Debart, F.; Vasseur, J.J.; Grimes, J.M.; Decroly, E.
    The methyltransferase domain of the Sudan ebolavirus L protein specifically targets internal adenosines of RNA substrates, in addition to the cap structure (2018), Nucleic Acids Res., 46, 7902-7912 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.56

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli T7 cells Sudan ebolavirus

Protein Variants

Protein Variants Comment Organism
D1924A the mutant shows severely reduced activity compared to the wild type enzyme Sudan ebolavirus
E1996A the mutant shows severely reduced activity compared to the wild type enzyme Sudan ebolavirus
K1813A the mutant shows severely reduced activity compared to the wild type enzyme Sudan ebolavirus
K1959A the mutant shows severely reduced activity compared to the wild type enzyme Sudan ebolavirus

Organism

Organism UniProt Comment Textmining
Sudan ebolavirus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Co-NTA resin column chromatography Sudan ebolavirus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the protein reveals cap-independent methyltransferase activities targeting internal adenosine residues. Besides this, the enzyme domain also methylates the N7 position of the cap guanosine. The enzyme preferentially methylates adenosine residues and is not active on poly G, C and U RNAs Sudan ebolavirus ?
-
-
S-adenosyl-L-methionine + GpppGAU-[RNA] strong activity Sudan ebolavirus ?
-
 ?
S-adenosyl-L-methionine + mGpppGAU-[RNA] strong activity Sudan ebolavirus ?
-
 ?
S-adenosyl-L-methionine + mGpppGmAmU-[RNA] strong activity Sudan ebolavirus ?
-
 ?
S-adenosyl-L-methionine + mGpppGmAU-[RNA] strong activity Sudan ebolavirus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 59000, SDS-PAGE Sudan ebolavirus

Synonyms

Synonyms Comment Organism
methyltransferase domain of Sudan ebolavirus L protein
-
 Sudan ebolavirus
MTase+CTD
-
 Sudan ebolavirus