EC Number   |
Protein Variants |
Reference |
|---|
   2.1.1.56 | C173A/S174A |
modest defect, grows well at 19, 30 and 34°C, but very slowly at 37°C |
677246 |
| 2.1.1.56 | C382Y |
15-50% of wild-type activity |
735335 |
| 2.1.1.56 | C382Y |
72% of wild-type methylation activity |
734586 |
| 2.1.1.56 | C414R |
complete loss of methylation activity |
734586 |
| 2.1.1.56 | C414R |
mutation abolishes the N7-MTase activity on GTP |
735335 |
| 2.1.1.56 | C416R |
mutation abolishes the N7-MTase activity on GTP |
735335 |
| 2.1.1.56 | D122A/S123A |
site-directed mutagenesis, complementation of the yeast abd1DELTA mutant at 25°C and 30°C, only slightly at 37°C |
659921 |
| 2.1.1.56 | D144A |
TbCmt1 mutant shows 5% of wild-type activity |
677098 |
| 2.1.1.56 | D146A |
mutant completely loses the N-7 activity (methylation of N-7 position of guanine) and 2'-O methylation activity. Mutation defective in N-7 methylation is lethal for DNV replication |
721100 |
| 2.1.1.56 | D1924A |
the mutant shows severely reduced activity compared to the wild type enzyme |
757853 |
