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Search term: molecular biology

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EC Number Recommended Name Application Commentary
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.41polypeptide N-acetylgalactosaminyltransferase molecular biology enzyme-linked lectin assay (ELLA) as carbohydrate-binding assay
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.41polypeptide N-acetylgalactosaminyltransferase molecular biology invariant residue Trp328 is essential for GalNAc-T enzymatic activity, residue Trp316 is important in the interaction with the acceptor polypeptide of GalNAc-T1
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.41polypeptide N-acetylgalactosaminyltransferase molecular biology overexpression in eukaryotic cell culture leads to inhibition of Activin/Nodal pathway activity in vivo by interference with binding of ActR-IIB to type I TGFbeta receptor proteins that can mediate BMP as well as Nodal signalling
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.41polypeptide N-acetylgalactosaminyltransferase molecular biology polyclonal rabbit anti-GalNAc-T14 IgG (1: 1000 in Western blot, titer: appr. 1: 16000) for study of expression and distribution of human GalNAc-T14
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.653-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase molecular biology the stable system using the expression vector pIB/Vf-His-TOPO constitutes an advance for the large scale expression of glycosyltransferases and possibly other glycoproteins in insect cells
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.80ceramide glucosyltransferase molecular biology glucosylceramide is essential for MsDef1-mediated growth inhibition of Fusarium graminearum, but not for its pathogenicity
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.101alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase molecular biology production of rare hybrid oligosaccharides for biochemical and structural studies, 100% conversion of oligosaccharide substrate at room temperature, yield of 42% after purification from reaction mixture
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.102beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase molecular biology beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-p-nitrophenyl and other nitrophenyl-sugar-derivatives are useful as specific inhibitors and as affinity label
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.221peptide-O-fucosyltransferase molecular biology engineering of an O-fucosylation system in yeast provides a powerful tool for producing proteins with homogenous carbohydrate chains. Such proteins can be used for the analysis of substrate specificity and the production of antibodies that recognize O-glycosylated EGF domains
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.221peptide-O-fucosyltransferase molecular biology O-fucosylation is dispensable for many Notch signaling events during Drosophila development
Show all pathways known for 2.4.2.8Display the word mapDisplay the reaction diagram Show all sequences 2.4.2.8hypoxanthine phosphoribosyltransferase molecular biology HPRT mutations in vivo in human T-lymphocytes are useful probes for mechanistic investigations, molecular analyses of isolated mutants reveal their underlying mutational changes as well as the T-cell receptor gene rearrangements present in the cells in question, overview
Show all pathways known for 2.4.2.8Display the word mapDisplay the reaction diagram Show all sequences 2.4.2.8hypoxanthine phosphoribosyltransferase molecular biology the HPRT gene is used as reporter gene in HL-60 cells for investigation of the mutagenic potential of succinyl-acetone by determining the frequencies of somatic mutations in the HPRT reporter gene, overview
Show all pathways known for 2.4.2.9Display the word mapDisplay the reaction diagram Show all sequences 2.4.2.9uracil phosphoribosyltransferase molecular biology engineering the yeast fluorocytosine deaminase (FCY1) gene by creating a fusion with the bacterial uracil phosphoribosyl transferase (UPP) gene results in a recombinant protein that converts the precursor 5-fluorocytosine (5-FC) into 5-fluorouracyl, a drug used in the treatment of a range of cancers, which triggers DNA and RNA damage. The tissue-specific FCY-UPP system is a great tool to inactivate cells in a precise spatial and temporal manner, method evaluation, overview
Show all pathways known for 2.4.2.30Display the word mapDisplay the reaction diagram Show all sequences 2.4.2.30NAD+ ADP-ribosyltransferase molecular biology establishment of an immortalized PARP-1-/- murine endothelial cell line HYKO6 as a tool to study PARP-1-mediated endothelial cell dysfunction
Show all pathways known for 2.5.1.10Display the word mapDisplay the reaction diagram Show all sequences 2.5.1.10(2E,6E)-farnesyl diphosphate synthase molecular biology farnesyl pyrophosphate synthase as a target for fragment-based lead discovery has revealed that it can be used for fragment library screening and hit validation using an unconventional referencing, suitable when reference compounds are not available
Show all pathways known for 2.5.1.15Display the word mapDisplay the reaction diagram Show all sequences 2.5.1.15dihydropteroate synthase molecular biology established coupled enzymatic assay for kinetic analyses of DHPS activity (coupled to pyrophosphate-dependent phosphofructokinase, aldolase, triosephosphate isomerase, alpha-glycerophosphate dehydrogenase) in presence or absence of activity-modulating compounds
Display the word mapDisplay the reaction diagram Show all sequences 2.5.1.20rubber cis-polyprenylcistransferase molecular biology model for rubber biosynthesis
Display the word mapDisplay the reaction diagram Show all sequences 2.5.1.59protein geranylgeranyltransferase type I molecular biology the GGTase-I variants with altered substrate specificity can serve as tools for studying GGTase-I substrate selectivity and the effects of prenylation pathway modifications on specific proteins
Show all pathways known for 2.5.1.78Display the word mapDisplay the reaction diagram Show all sequences 2.5.1.786,7-dimethyl-8-ribityllumazine synthase molecular biology outside of the cell, the hollow spherical architecture of the enzyme capsid is used as a template for the encapsulation of cargo proteins, such as green fluorescent proteins, and HIV proteases, and fabrication of uniform layer-by-layer assemblies using non-covalent interactions between surface-displayed His6 and Ni-NTA of enzyme AaLS. The enzyme shows encapsulation capability and surface presentation of ligands, which represent the great potential of AaLS as a versatile delivery vehicle
Show all pathways known for 2.6.1.19Display the word mapDisplay the reaction diagram Show all sequences 2.6.1.194-aminobutyrate-2-oxoglutarate transaminase molecular biology Agrobacterium tumefaciens is used to mediate inter-kingdom DNA transfer in plant genetic engineering. Gamma-aminobutyric acid (GABA) is a negative factor in the Agrobacterium-plant interaction, because it inhibits the DNA transfer. Generation of an Agrobacterium tumefaciens strain expressing the Escherichia coli gene gabT, which introduces GABA transaminase activity and the ability to degrade GABA, is achieved to circumvent the inhibitory effect of GABA
Show all pathways known for 2.7.1.21Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.21thymidine kinase molecular biology a method to distinguish between the de novo induction of thymidine kinase mutants and the selection of pre-existing thymidine kinase mutants in the mouse lymphoma assay
Show all pathways known for 2.7.1.60Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.60N-acylmannosamine kinase molecular biology GNE-deficient cells, with dramatically increased incorporation of N-acetylmannosamine analogues into glycoproteins, can efficiently be decorated with reactive functional groups, which can be employed in bioorthogonal functionalization strategies for fluorescence labelling or biotinylation
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.119hygromycin-B 7''-O-kinase molecular biology use the hygromycin phosphotransferase gene (hpt) as a selective marker gene for tracking plastid transformation in rice (Oryza sativa)
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.163hygromycin B 4-O-kinase molecular biology the enzyme can be used as selective marker gene product in production of transgenic plants
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.163hygromycin B 4-O-kinase molecular biology the mutant gene hph5 gene can be used as a selection marker in the host-vector system of Thermus thermophilus either on plasmid or by genome integration
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.163hygromycin B 4-O-kinase molecular biology as selective marker gene
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.163hygromycin B 4-O-kinase molecular biology gene is used as selectable marker, mediates hygromycin resistence
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.163hygromycin B 4-O-kinase molecular biology gene sequence is used as selectable marker, mediates hygromycin resistance
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.163hygromycin B 4-O-kinase molecular biology hpt gene is used as a selectable marker
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.163hygromycin B 4-O-kinase molecular biology hpt gene is used as selectable marker, mediates hygromycin resistance
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.163hygromycin B 4-O-kinase molecular biology resistance against hyromycin B mediated by transformation of the hph gene
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.163hygromycin B 4-O-kinase molecular biology resistance against hyromycin B mediated by transformation of the hph gene, a selectable marker gen.
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.163hygromycin B 4-O-kinase molecular biology selectable marker (SM) genes, essential to select transformed cells from a large population of untransformed cells
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.163hygromycin B 4-O-kinase molecular biology Used as marker gene mediating hygromycin resistance.
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.163hygromycin B 4-O-kinase molecular biology Used as marker gene. Mediates hygromycin resistance.
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.190aminoglycoside 2''-phosphotransferase molecular biology there is a need to develop alternative markers for plastid transformation to (a) extend the species range of the technology, and (b) facilitate the multistep engineering of plastid genomes, for example, by sequential introduction of multiple transgenes (supertransformation). Bifunctional aminoglycoside acetyltransferase/phosphotransferase conferring tobramycin resistance provides an efficient selectable marker for stable plastid transformation
Show all pathways known for 2.7.4.1Display the word mapDisplay the reaction diagram Show all sequences 2.7.4.1ATP-polyphosphate phosphotransferase molecular biology power of ppk1 as a genetic marker for detection of all currently defined Candidatus Accumulibacter clades
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.6DNA-directed RNA polymerase molecular biology Rpo41-Mtf1 is an attractive candidate for serving as the primase to initiate lagging strand DNA synthesis during normal replication and/or to restart stalled replication from downstream ssDNA
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.6DNA-directed RNA polymerase molecular biology usage of enzyme RNAP to systematically decipher the black matter and identify giant viruses. As they lack ribosomal genes, these giant viruses are not part of the rDNA tree. A relevant alternative to rDNA for microbe identification are DNA-dependent RNA polymerase (RNAP) genes. They are more refractory to lateral gene transfers and usually present in a single copy in genomes, which avoids recombination and issues related to divergence between copies
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.7DNA-directed DNA polymerase molecular biology thermostable polymerase used in PCR
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.7DNA-directed DNA polymerase molecular biology generation of a unique one enzyme system with high fidelity to allow highly accurate and efficient amplification of DNA targets using polymerase chain reaction by fusing Sso7d protein to Tpa DNA polymerase
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.7DNA-directed DNA polymerase molecular biology Tce polymerase may be useful in DNA amplification requiring high fidelity
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.7DNA-directed DNA polymerase molecular biology the high fidelty of the enzyme is suitable fo polymerase chain reaction (PCR), which requires accurate DNA amplification for gene cloning and diagnostic assay
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.7DNA-directed DNA polymerase molecular biology long and accurate PCR can be achieved with a mixture of wild type DNA polymerase from Thermococcus kodakaraensis and its exonuclease deficient mutant enzyme N210D is utilized (at the ratio of 1:40)
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.7DNA-directed DNA polymerase molecular biology optimal conditions for polymerase chain reaction are determined. Iho DNA polymerase possesses 3'->5' exonuclease activity, and the fidelity of the Iho DNA polymerase is similar to that of Pfu and Vent DNA polymerases. However, Iho DNA polymerase provides more enhanced efficiency of PCR amplification than Pfu and Vent DNA polymerases. Iho DNA polymerase can successfully amplify a 2-kb lambda DNA target with a 10/s extension time and could amplify a DNA fragment up to 8 kb lambda DNA
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.7DNA-directed DNA polymerase molecular biology the H633R mutant DNA polymerase may be useful in high-fidelity DNA amplification and various PCR-based applications
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.7DNA-directed DNA polymerase molecular biology application for long and accurate PCR. The PCR error rate of the Tba5 DNA polymerase plus4 (Tba5 plus DNA polymerase mixtures are constituted with various amounts of Tba5 DNA polymerase mixed with Taq DNA polymerase) is much lower than that of the wild-type enzyme alone
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.7DNA-directed DNA polymerase molecular biology the enzyme can be used in routine PCR
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.7DNA-directed DNA polymerase molecular biology the thermostable properties of the enzyme from Thermus aquaticus have contributed majorly to the specificity, automation, and efficacy of the polymerase chain reaction (PCR)
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.8polyribonucleotide nucleotidyltransferase molecular biology targeted overexpression of hPNPase represents a strategy to selectively downregulate RNA expression and consequently intervene in a variety of pathophysiological conditions
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.B22transposase molecular biology Sleeping Beauty is a prominent Tc1/mariner superfamily DNA transposon that provides a popular genome engineering tool in a broad range of organisms. It is mobilized by a transposase enzyme that catalyses DNA cleavage and integration at short specific sequences at the transposon ends
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.B22transposase molecular biology the maize activator (Ac) transposase recognizes and excises Ac and Dissociation (Ds) elements and mediates insertion elsewhere in the genome. Insertions of Ds can cause disruption in gene sequences and hence are important functional genomics tool for tagging and cloning of unknown gene sequences
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.B22transposase molecular biology transposases are important tools in genome engineering. The first DNA transposon tool capable for gene transfer in vertebrates is Sleeping Beauty (SB), which is reconstructed from extinct Tc1/mariner transposons in fish. Sleeping Beauty, and especially its hyperactive variant is still one of the most widely used transposon tools, in human clinical trials
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.48RNA-directed RNA polymerase molecular biology the enzyme, that is absent in mammalian cells, enhances the effect of siRNA expression on target gene expression in mammalian cells, when coexpressed, for amplifying the use of hairpin siRNA expression vectors for gene silencing, method development, overview
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.64UTP-monosaccharide-1-phosphate uridylyltransferase molecular biology polyclonal rabbit anti-AtUSP antibody recognizes USP1 and USP2
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.65diguanylate cyclase molecular biology generation of structural models for product-inhibited, elongated dimer
Show all pathways known for 2.7.8.7Display the word mapDisplay the reaction diagram Show all sequences 2.7.8.7holo-[acyl-carrier-protein] synthase molecular biology MtaA should be a usefool tool for activating heterologously expressed polyketide synthase and nonribosomal polyketide synthase systems
Show all pathways known for 2.7.8.7Display the word mapDisplay the reaction diagram Show all sequences 2.7.8.7holo-[acyl-carrier-protein] synthase molecular biology insights in molecular architecture and reaction mechanism of group II PPTs in contrast to group I PPTs (bacterial) enable screening for antibacterial agents which specifically inhibit bacterial PPTs
Show all pathways known for 2.7.8.7Display the word mapDisplay the reaction diagram Show all sequences 2.7.8.7holo-[acyl-carrier-protein] synthase molecular biology SchPPT is a promiscuous PPTase and may be used on polyketide production in heterologous bacterial host and labeling of acyl-carrier proteins, ACPs
Show all pathways known for 2.7.8.13Display the word mapDisplay the reaction diagram Show all sequences 2.7.8.13phospho-N-acetylmuramoyl-pentapeptide-transferase molecular biology synthesis of radiolabelled UDP-MurNAc-pentapeptide as biochemical tools for studying peptidoglycan biosynthesis or the kinetic characterization of MraY
Show all pathways known for 2.7.8.15Display the word mapDisplay the reaction diagram Show all sequences 2.7.8.15UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase molecular biology combination of GPT and tunicamycin is a potential selectable marker system for potato transformation, overview
Display the word mapDisplay the reaction diagram Show all sequences 2.7.11.1non-specific serine/threonine protein kinase molecular biology AcMNPV-pk-1 is a component of the viral very late gene transcription initiation complex
Display the word mapDisplay the reaction diagram Show all sequences 2.7.11.1non-specific serine/threonine protein kinase molecular biology EmbR2 is a regulator of PknH activation, thus directly participating in the control of the PknH/EmbR pair
Display the word mapDisplay the reaction diagram Show all sequences 2.7.11.1non-specific serine/threonine protein kinase molecular biology pknA and pknB are key players in signal transduction pathways for the regulation of the cell shape and both are essential for sustaining corynebacterial growth
Display the word mapDisplay the reaction diagram Show all sequences 2.7.11.1non-specific serine/threonine protein kinase molecular biology results demonstrate that MPK38 physically interacts with ASK1 in vivo and acts as a positive upstream regulator of ASK1
Display the word mapDisplay the reaction diagram Show all sequences 2.7.11.1non-specific serine/threonine protein kinase molecular biology results display the requirement of RSK activity during Artemia development and suggest its role in termination of cell cycle (G2/Mphase) arrest and promotion of mitogenesis
Display the word mapDisplay the reaction diagram Show all sequences 2.7.11.1non-specific serine/threonine protein kinase molecular biology SAPK4 regulates ion homeostasis and growth and development under salinity and this indicates a function of SAPK4 as a regulatory factor in plant salt stress acclimation
Display the word mapDisplay the reaction diagram Show all sequences 2.7.11.1non-specific serine/threonine protein kinase molecular biology STK38 interacts with the MAPKKKs MEKK1 and MEKK2 (MEKK1/2). STK38 inhibits MEKK1/2 activation without preventing MEKK1/2 binding to its substrate, SEK1. STK38 suppresses the autophosphorylation of MEKK2 without interfering with MEKK2 dimer formation, and converts MEKK2 from its phosphorylated to its nonphosphorylated form. The negative regulation of MEKK1/2 is not due to its phosphorylation by STK38. STK38 negatively regulates the activation of MEKK1/2 by direct interaction with the catalytic domain of MEKK1/2, suggesting a novel mechanism of MEKK1/2 regulation
Display the word mapDisplay the reaction diagram Show all sequences 2.7.11.10IkappaB kinase molecular biology IKK-alpha is a potential target for manipulating TLR-induced IFN-alpha production
Display the word mapDisplay the reaction diagram Show all sequences 2.7.11.10IkappaB kinase molecular biology IKK/nuclear factor-kappaB dependent microglia activation contributes to KA-induced hippocampal neuronal cell death in vivo through induction of inflammatory mediators
Display the word mapDisplay the reaction diagram Show all sequences 2.7.11.10IkappaB kinase molecular biology IKKalpha and IKKbeta exert differential roles in ECM remodeling and endochondral ossification, which are events characteristic of hypertrophic chondrocytes and also complicating factors often found in osteoarthritic chondrocytes
Display the word mapDisplay the reaction diagram Show all sequences 2.7.11.10IkappaB kinase molecular biology it is shown that mTOR and Raptor, through an Akt-dependent pathway, control NF-kappaB activity in PC3 prostate cancer cells both at the level of IkappaBalpha and RelA/p65 phosphorylation
Display the word mapDisplay the reaction diagram Show all sequences 2.7.11.10IkappaB kinase molecular biology NF-kappaB is activated by alloferon through down-regulation of antioxidant proteins and IkappaBalpha
Display the word mapDisplay the reaction diagram Show all sequences 2.7.11.10IkappaB kinase molecular biology regulation of IKKbeta by miR-199a affects NF-kappaB activity in ovarian cancer cells
Display the word mapDisplay the reaction diagram Show all sequences 2.7.11.10IkappaB kinase molecular biology tetrandrine suppresses LPS-induced astrocyte activation via modulating IKKs-IkappaBalpha-NF-kappaB signaling pathway
Display the word mapDisplay the reaction diagram Show all sequences 2.7.11.13protein kinase C molecular biology a technique is developed to detect PKCalpha activity in a cancerous cell lysate through the simple measurement of fluorescence intensity. The principle of this methodology is based on a fluorescence increase associated with polyion complex dissociation due to phosphorylation by PKCalpha
Display the word mapDisplay the reaction diagram Show all sequences 2.8.2.20protein-tyrosine sulfotransferase molecular biology conjugation of proteins with N-carbamoyl-succinate-modified peptides is an appropriate tool in research, for instance, in the development of vaccines and drugs or for studying biological mechanisms
Show all pathways known for 2.8.3.8Display the word mapDisplay the reaction diagram Show all sequences 2.8.3.8acetate CoA-transferase molecular biology a new degenerated real-time PCR approach to simultaneously quantify phylogenetically different butyrate-producing bacteria based on the detection of butyryl-coenzyme A (CoA) CoA transferase genes is described
Display the word mapDisplay the reaction diagram Show all sequences 2.8.3.16formyl-CoA transferase molecular biology use of the frc gene as template for PCR to detect oxalotrophic bacteria
Display the word mapDisplay the reaction diagram Show all sequences 3.1.1.6acetylesterase molecular biology postulated PON (paraoxonase) family membership due to similarity to primary structure of PON2, plant strictosidine synthase and di-isopropyl fluorophosphatase, its N-terminal single transmembrane domain, a nine-exon gene structure, a six-bladed beta-propeller tertiary structure, and similar metabolic regulation of gene expression
Display the word mapDisplay the reaction diagram Show all sequences 3.1.1.6acetylesterase molecular biology protein-protein interaction consensus sequence, involved in regulation of both sugar and lipid metabolism, according to interaction partners
Show all pathways known for 3.1.1.17Display the word mapDisplay the reaction diagram Show all sequences 3.1.1.17gluconolactonase molecular biology coimmobilization with glucose oxidase in polyelectrolyte gels for improvement of kinetic properties, active enzymes in the gel undergo a shrinking process due to a sudden drop in pH, gel volume phase transition, overview
Display the word mapDisplay the reaction diagram Show all sequences 3.1.1.53sialate O-acetylesterase molecular biology origin and evolution of viral hemagglutinin-esterases
Display the reaction diagram Show all sequences 3.1.1.56methylumbelliferyl-acetate deacetylase molecular biology regulation of serine esterase by sulfenic acid found at C60 in mutant W197I (PDB: 3C6B)
Display the reaction diagram Show all sequences 3.1.1.56methylumbelliferyl-acetate deacetylase molecular biology studying the physiological role
Display the word mapDisplay the reaction diagram Show all sequences 3.1.1.96D-aminoacyl-tRNA deacylase molecular biology the dtd (yrvI) gene from B. amyloliquefaciensA50, encoding the putative metabolite proofreading enzyme D-tyrosyl-tRNATyr deacylase, is associated with resistance to the non-canonical amino acid D-tyrosine. This gene can be applied as a convenient, small selectable marker for non-antibiotic resistance selection in experiments aimed at genome editing of D-Tyr-sensitive microorganisms
Display the word mapDisplay the reaction diagram Show all sequences 3.1.1.117(4-O-methyl)-D-glucuronate---lignin esterase molecular biology the enzyme may prove a valuable as research tool for the investigation of lignin and lignin to carbohydrates-bond chemistry
Display the word mapDisplay the reaction diagram Show all sequences 3.1.1.117(4-O-methyl)-D-glucuronate---lignin esterase molecular biology the enzyme may prove a valuable research tool for the investigation of lignin and lignin to carbohydrates-bond chemistry
Display the word mapDisplay the reaction diagram Show all sequences 3.1.1.117(4-O-methyl)-D-glucuronate---lignin esterase molecular biology the enzyme may prove valuable as a research tool for the investigation of lignin and lignin to carbohydrates-bond chemistry
Show all pathways known for 3.1.3.1Display the word mapDisplay the reaction diagram Show all sequences 3.1.3.1alkaline phosphatase molecular biology analytically widely used enzyme, e.g. in ELISA, enzyme-linked immunosorbent assay
Show all pathways known for 3.1.3.1Display the word mapDisplay the reaction diagram Show all sequences 3.1.3.1alkaline phosphatase molecular biology widely used in vitro, e.g. to dephosphorylate DNA or dNTPs, since the enzyme can be inactivated by a short rise in temperature
Show all pathways known for 3.1.3.1Display the word mapDisplay the reaction diagram Show all sequences 3.1.3.1alkaline phosphatase molecular biology the purified alkaline phosphatase removes the 5'-phosphate group of a linearized plasmid without showing DNAase activity, indicating its potential for recombinant DNA technology
Show all pathways known for 3.1.3.1Display the word mapDisplay the reaction diagram Show all sequences 3.1.3.1alkaline phosphatase molecular biology BAP may play an important role in differentiation and maturation of human B cells
Show all pathways known for 3.1.3.1Display the word mapDisplay the reaction diagram Show all sequences 3.1.3.1alkaline phosphatase molecular biology colchicine inhibits the dexamethasone-promoted translocation of ALP to the plasma membrane surrounding the bile canaliculus-like structure in primary cultures of fetal rat hepatocytes by disassembling microtubules and discomposing the Golgi complex
Show all pathways known for 3.1.3.1Display the word mapDisplay the reaction diagram Show all sequences 3.1.3.1alkaline phosphatase molecular biology influence of membrane lipid environment on the activity of GPI-anchored enzymes is investigated with PLAP reconstituted by a detergent-dialysis technique in liposomes composed of palmitoyloleoylphosphatidylcholine, alone or in mixture with lipids enriched along with the protein within lipid rafts. The highest Vmax is recorded for a phosphatidylcholine/10% monosialoganglioside, while the lowest for a phosphatidylcholine/30% cholesterol mixture and for raft-mimicking 1:1:1 phosphatidylcholine/sphingolipid/cholesterol liposomes. No significant differences in Km are detected. The GPI-enzyme activity is affected by membrane curvature
Show all pathways known for 3.1.3.1Display the word mapDisplay the reaction diagram Show all sequences 3.1.3.1alkaline phosphatase molecular biology relationship between extracellular pH and the release of alkaline phosphatase using stimuli affecting extracellular pH, i.e. heat shock, nigericin, salicylicacid, benzoic acid, fusicoccin, NaOH, KOH, HCl, and the Golgi apparatus inhibitor BrefeldinA is investigated. Extracellular alkaline phosphatase is a sensitive marker for cellular stimulation
Show all pathways known for 3.1.3.2Display the word mapDisplay the reaction diagram Show all sequences 3.1.3.2acid phosphatase molecular biology AcP and cysteine protease cooperate to assure vitellin breakdown during early embryogenesis of Periplaneta americana
Show all pathways known for 3.1.3.2Display the word mapDisplay the reaction diagram Show all sequences 3.1.3.2acid phosphatase molecular biology ACP5 possesses a central role in removal of the mannose 6-phosphate recognition marker
Show all pathways known for 3.1.3.2Display the word mapDisplay the reaction diagram Show all sequences 3.1.3.2acid phosphatase molecular biology APase activity may affect the tuber swelling by partially regulating the sucrose-mediated sugar resorption
Show all pathways known for 3.1.3.2Display the word mapDisplay the reaction diagram Show all sequences 3.1.3.2acid phosphatase molecular biology the localization of an ACP in the arbuscular mycorrhizal (AM) interface of arbusculate coils suggests that this enzyme may be involved in the phosphorus efflux from themycorrhizal fungus to the host
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