Information on EC 2.4.1.41 - polypeptide N-acetylgalactosaminyltransferase

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The expected taxonomic range for this enzyme is: Eukaryota

EC NUMBER
COMMENTARY hide
2.4.1.41
-
RECOMMENDED NAME
GeneOntology No.
polypeptide N-acetylgalactosaminyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-N-acetyl-alpha-D-galactosamine + polypeptide = UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
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mucin core 1 and core 2 O-glycosylation
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-
mucin core 3 and core 4 O-glycosylation
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-
Mucin type O-Glycan biosynthesis
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-
SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyl-transferase
Requires both Mn2+ and Ca2+. The glycosyl residue is transferred to threonine or serine hydroxy groups on the polypeptide core of submaxillary mucin, kappa-casein, apofetuin and some other acceptors of high molecular mass.
CAS REGISTRY NUMBER
COMMENTARY hide
9075-15-4
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
several isozymes
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-
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
isozymes xGalnt1, -4, -6, -7
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-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
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GalNAc-type protein O-glycosylation is important in modulating protein processing, O-linked glycans have important biological functions
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
erythropoietin T + UDP-Gal
erythropoietin T-nGalNAc + UDP
show the reaction diagram
hCG-beta + UDP-Gal
hCG-beta-nGalNAc + UDP
show the reaction diagram
-
-
-
?
IgA hinge + UDP-Gal
IgA hinge-nGal + UDP
show the reaction diagram
-
-
-
?
IgA hinge + UDP-GalNAc
IgA hinge-nGalNAc + UDP
show the reaction diagram
IgA hinge-4GalNAc + UDP-GalNAc
IgA hinge-nGalNAc + UDP
show the reaction diagram
-
-
-
?
MUC1 + UDP-GalNAc
MUC1-nGalNAc + UDP
show the reaction diagram
MUC1-2GalNAc + UDP-GalNAc
MUC1-nGalNAc + UDP
show the reaction diagram
-
-
-
?
MUC1a' + UDP-GalNAc
MUC1a'-nGalNAc + UDP
show the reaction diagram
-
-
-
?
MUC1b' + UDP-GalNAc
MUC1b'-nGalNAc + UDP
show the reaction diagram
-
-
-
?
MUC2 + UDP-GalNAc
MUC2-nGalNAc + UDP
show the reaction diagram
-
-
-
?
MUC2-6GalNAc + UDP-GalNAc
MUC2-nGalNAc + UDP
show the reaction diagram
-
-
-
?
MUC5AC + UDP-GalNAc
GalNAc-Thr9-MUC5AC + UDP
show the reaction diagram
-
hT2CD, hT2: glycosylation site selection driven by and substrate binding through its catalytic domain, hT10: not catalysed by hT10 because hT10 does not recognize naked peptides
preferred site for glycosylation by hT2 and hT2CD compared to Thr-3, Thr-10 and Thr-13
-
?
MUC5AC glycopeptides + UDP-GalNAc
GalNAc-MUC5AC + UDP
show the reaction diagram
-
hT2: site preference of glycosylation depends on location of pre-existing GalNAc and is mediated by its lectin domain which directs glycosylation 10 residues N- or C-terminal to an extant GalNAc residue and aids site selection only when potential site is N-terminal to an extant GalNAc residue not C-terminal, Thr-9 is no preferred site of glycosylation, hT10: must recognize existing GalNAc residues via its catalytic domain which directs selection to the glycosylation site immediately N-terminal to the present GalNAc,
-
-
?
MUC5AC-13 + UDP-GalNAc
GalNAc-Thr12-MUC5AC-13 + UDP
show the reaction diagram
-
hT10, hT10CD, hT10CD-hT2LD
-
-
?
MUC5AC-13 + UDP-GalNAc
GalNAc-Thr3-MUC5AC-13 + UDP
show the reaction diagram
-
hT2: substrate binding through its catalytic domain, hT10: transfer of only single GalNAc residues
predominant product of hT2 and hT10
-
?
MUC5AC-13 + UDP-GalNAc
GalNAc-Thr9-MUC5AC-13 + UDP
show the reaction diagram
-
hT2, hT2CD: no initial burst phase during catalysis
not preferred by hT2, preferred by hT2CD
-
?
MUC5AC-3 + UDP-GalNAc
GalNAc-Thr13-MUC5AC-3 + UDP
show the reaction diagram
-
hT2: no initial burst phase during catalysis, substrate binding through its catalytic domain, hT10: transfer of only single GalNAc residues
hT2: preferred site for glycosylation, mediated by enhanced product release through lectin domain, hT10: predominant product
-
?
MUC5AC-3 + UDP-GalNAc
GalNAc-Thr2-MUC5AC + UDP
show the reaction diagram
-
hT10CD, hT10CD-hT2LD, hT10: catalysis faster than for MUCAC-9
-
-
?
MUC5AC-3 + UDP-GalNAc
GalNAc-Thr9-MUC5AC-3 + UDP
show the reaction diagram
-
hT2, hT2CD
not preferred by hT2, hT2CD: preferred site for glycosylation, lack of lectin domain in hT2CD results in rate-limiting product release
-
?
MUC5AC-3,13 + UDP-GalNAc
GalNAc-Ser-5-MUC5AC-3,13 + UDP
show the reaction diagram
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hT2, not catalysed by hT2CD and hT2CD-hT10LD
preferred site by hT2
-
?
MUC5AC-3,13 + UDP-GalNAc
GalNAc-Thr2,12-MUC5AC-3,13 + UDP
show the reaction diagram
-
hT10, hT10CD, hT10CD-hT2LD
-
-
?
MUC5AC-9 + UDP-GalNAc
GalNAc-Thr2-MUC5AC-9 + UDP
show the reaction diagram
-
not catalysed by hT10CD and hT10CD-hT2LD, hT10: catalysis slower than for MUC5AC-3
preferred site of glycosylation by hT10
-
?
MUC7 + UDP-GalNAc
MUC7-nGalNAc + UDP
show the reaction diagram
-
-
-
?
OSM fragment + UDP-Gal
OSM fragment-nGalNAc + UDP
show the reaction diagram
-
-
-
?
UDP-alpha-GalNAc + polypeptide
GalNAc-alpha-polypeptide + UDP
show the reaction diagram
-
major mucin-type O-glycan in embryonic tracheal system along the apical region and luman of the tracheal tubes O-glycan, detectable by lectins Jacalin, soybean agglutinin and Vicia villosa agglutinin
-
?
UDP-galactose + Muc1 peptide
UDP + galactosyl-Muc1 peptide
show the reaction diagram
UDP-galactose + Muc2 peptide
UDP + galactosyl-Muc2 peptide
show the reaction diagram
UDP-galactose + PTTTPITTTTK
UDP + galactosyl-PTTTPITTTTK
show the reaction diagram
UDP-GalNAc + Ac-TPPA
UDP + Ac-(GalNAcalpha1-)TPPA
show the reaction diagram
-
pH 7.5, 37C
-
-
?
UDP-GalNAc + apomucin
UDP + GalNAc-mucin
show the reaction diagram
apomucin is a deglycosylated bovine submaxillary mucin, mutants W328Y, W328F, W328A, W316H and W316A are inactive, mutants W316Y and W316F show 40% and 20% activity, respectively, relative to wild-type
-
-
?
UDP-GalNAc + AQPTPPP
UDP + AQP-(GalNAcalpha1-)TPPP
show the reaction diagram
-
pH 7.5, 37C, 0.2 mM, 100% activity
-
-
?
UDP-GalNAc + EA2
GalNAc-EA2 + UDP
show the reaction diagram
UDP-GalNAc + GAGA(X)3T(X)3AGAGK
GAGA(X)3(GalNAc)T(X)3AGAGK + UDP
show the reaction diagram
-
-
-
?
UDP-GalNAc + GAGA(X)3T(X)3AGAGK
UDP + GAGA(X)3(GalNAc)T(X)3AGAGK
show the reaction diagram
UDP-GalNAc + GAGA(X)5T(X)5AGAGK
GAGA(X)5(GalNAc)T(X)5AGAGK + UDP
show the reaction diagram
UDP-GalNAc + GAGA(X)5T(X)5AGAGK
UDP + GAGA(X)5(GalNAc)T(X)5AGAGK
show the reaction diagram
UDP-GalNAc + GAGA(X)nT(X)nAGAGK
GAGA(X)n(GalNAc)T(X)nAGAGK + UDP
show the reaction diagram
UDP-GalNAc + GVVPTVVPG
UDP + GVVP(GalNAc)TVVPG
show the reaction diagram
GVVPTVVPG is a synthetic peptide with threonine as acceptor site, weaker acceptor substrate for wild-type and truncated version P-T1/delta42 as PPDAATAAPL
-
-
?
UDP-GalNAc + HGVTSAPDTRPAPGSTAPPA
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-GalNAc + N-acetyl-4-benzoyl-L-Phe-L-Pro-L-Tyr-L-Pro-L-Pro-L-Pro-NH2
UDP + N-acetyl-4-benzoyl-L-Phe-L-Pro-L-(GalNAcalpha1-)Tyr-L-Pro-L-Pro-L-Pro-NH2
show the reaction diagram
-
pH 7.5, 37C
-
-
?
UDP-GalNAc + polypeptide
GalNAc-alpha-polypeptide + UDP
show the reaction diagram
-
-
glycosylation at Thr or Ser
-
?
UDP-GalNAc + PPDAATAAPL
UDP + PPDAA(GalNAc)TAAPL
show the reaction diagram
PPDAATAAPL is a synthetic peptide with threonine as acceptor site, efficient acceptor substrate for wild-type and truncated version P-T1/delta42
-
-
?
UDP-GalNAc + PRFODSSSKAPPPLPSPSRLPG
GalNAc-PRFODSSSKAPPPLPSPSRLPG + UDP
show the reaction diagram
-
-
-
-
?
UDP-GalNAc + PTTTPISTTTMVTPTPTPTC
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-GalNAc + PTTTPITTTG
UDP + P-(GalNAcalpha1-)TTTPITTTG + P-(GalNAcalpha1-)TTTPI-(GalNAcalpha1-)TTTG
show the reaction diagram
-
pH 7.5, 37C, 0.2 mM, 26% activity relative to AQPTPPP
-
-
?
UDP-GalNAc + selectin
GalNAc-selectin + UDP
show the reaction diagram
-
-
reduced expression of cell surface E- and P-selectins (fourfold and fivefold) and L-selectins on immune cells of ppGalNAcT-1 deficient mice
-
?
UDP-GalNAc + TPTGTQTPTG
UDP + TPTG-(GalNAcalpha1-)TQTPTG
show the reaction diagram
-
pH 7.5, 37C, 0.2 mM, 8% activity relative to AQPTPPP
-
-
?
UDP-GalNAc + TTTVTPTPTG
UDP + TTTV-(GalNAcalpha1-)TPTPTG + T-(GalNAcalpha1-)TTV-(GalNAcalpha1-)TPTPTG + T-(GalNAcalpha1-)T-(GalNAcalpha1-)TV-(GalNAcalpha1-)TP-(GalNAcalpha1-)TPTG + T-(GalNAcalpha1-)TTV-(GalNAcalpha1-)TP-(GalNAcalpha1-)TP-(GalNAcalpha1-)TG + TT-(GalNAcalpha1-)TV-(GalNAcalpha1-)TPTPTG + ?
show the reaction diagram
-
pH 7.5, 37C, 0.2 mM, 109% activity relative to AQPTPPP
monoglycosylated product appears after 30 min, higher glycosylated products appear with increasing time of incubation
-
?
UDP-GalNAc + TVTPTPTPTG
UDP + TVTP-(GalNAcalpha1-)TPTPTG
show the reaction diagram
-
pH 7.5, 37C, 0.2 mM, 145% activity relative to AQPTPPP
-
-
?
UDP-GalNAc + VLTTTATTPTA
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + (glycosylated GTTPSPVPTTSTTSAP)
UDP + ?
show the reaction diagram
-
natural-type (alpha-GalNAc-O-Thr) and unnatural-type (beta-GalNAc-O-Thr, alpha-Fuc-O-Thr and beta-GlcNAc-O-Thr) glycosylated
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + Ac-CIRIQRGPGRAFVTIGKIGNMR
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + Ac-QATEYEYLDYDFLPETEPPEM
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + AHGVTSAPDTR
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + GAGAEAPTPAPAGAGK
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + GTTAKPTTLKPTE
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + GTTPSPVPTTSTTSAP
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + GTTPSPVPTTSTTSAP
UDP + GT[GalNAc]TPSPVPTTSTTSAP
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + GTTPSPVPTTSTTSAP
UDP + GT[GalNAc]TPSPVPTTST[GalNAc]TSAP
show the reaction diagram
-
-
glycosylation at Thr-3 and Thr-13
-
?
UDP-N-acetyl-alpha-D-galactosamine + GTTPSPVPTTSTTSAP
UDP + GT[GalNAc]TP[GalNAc]SPVPTTSTTSAP
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + GTTPSPVPTTST[GalNAc]TSAP
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + GTTPSPVPTTST[GalNAc]TSAP
UDP + GT[GalNAc]TPSPVPTTST[GalNAc]TSAP
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + GTTPSPVPTTST[GalNAc]TSAP
UDP + GT[GalNAc]TP[GalNAc]SPVPTTST[GalNAc]TSAP
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + GT[GalNAc]TPSPVPTTSTTSAP
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + GT[GalNAc]TPSPVPTTSTTSAP
UDP + GT[GalNAc]TPSPVPTTS[GalNAc]T[GalNAc]TSAP
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + GT[GalNAc]TPSPVPTTSTTSAP
UDP + G[GalNAc]T[GalNAc]TPSPVPTTST[GalNAc]TSAP
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + GT[GalNAc]TPSPVPTTST[GalNAc]TSAP
UDP + GT[GalNAc]TPSPVPTTS[GalNAc]T[GalNAc]TSAP
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + PDTRPAPGSTAPPAC
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + PSTPPTPSPSTPPTPSPS
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + PTTDSTTPAPTTK
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + SPTTSTPISSTPQPTS
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + A1 protein
UDP + N-acetyl-D-galactosaminyl-A1 protein
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + Ac-CIRIQRGPGRAFVTIGKIGNMR
UDP + N-acetyl-D-galactosaminylated Ac-CIRIQRGPGRAFVTIGKIGNMR
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-galactosamine + Ac-PFVTHPGYD
UDP + N-acetyl-D-galactosaminylated Ac-PFVTHPGYD
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-galactosamine + Ac-QATEYEYLDYDFLPETEPPEM
UDP + N-acetyl-D-galactosaminylated Ac-QATEYEYLDYDFLPETEPPEM
show the reaction diagram
UDP-N-acetyl-D-galactosamine + AcTPPP
UDP + N-acetyl-D-galactosaminyl-AcTPPP
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + AHGVTSAPDTR
UDP + N-acetyl-D-galactosaminylated AHGVTSAPDTR
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-galactosamine + AHGVVTSAPDTR
UDP + ?
show the reaction diagram
UDP-N-acetyl-D-galactosamine + ANTPSFPTATSPAPPI
UDP + ?
show the reaction diagram
UDP-N-acetyl-D-galactosamine + apoantifreeze glycoproteins
UDP + N-acetyl-D-galactosaminyl-apoantifreeze glycoproteins
show the reaction diagram
-
apoantifreeze glycoprotein of high molecular weight accepts GalNAc much faster than apoantifreeze glycoprotein 8
-
-
?
UDP-N-acetyl-D-galactosamine + apofetuin
UDP + N-acetyl-D-galactosaminyl-apofetuin
show the reaction diagram
-
lowest rate of transfer
-
-
?
UDP-N-acetyl-D-galactosamine + apomucin
UDP + N-acetyl-D-galactosaminyl-apomucin
show the reaction diagram
UDP-N-acetyl-D-galactosamine + AWIPTPPPIPG
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + bone sialoprotein
UDP + UDP-N-acetyl-D-galactosaminyl-bone sialoprotein
show the reaction diagram
-
preferred substrate of isozyme ppGalNAcT-1, glycosylation of Thr101, Ser131, Thr199, and Ser214, glycosylation pattern, overview
-
-
?
UDP-N-acetyl-D-galactosamine + CPPTPSATTPAPPSSSAPPETTAA
UDP + N-acetyl-D-galactosaminylated CPPTPSATTPAPPSSSAPPETTAA
show the reaction diagram
UDP-N-acetyl-D-galactosamine + deglycosylated ovine submaxillary mucin
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + DSTTPAPTTK
UDP + N-acetyl-D-galactosaminylated DSTTPAPTTK
show the reaction diagram
UDP-N-acetyl-D-galactosamine + DSTTPAPTTK
UDP + N-acetyl-D-galactosaminylated-DSTTPAPTTK
show the reaction diagram
mucin derived substrate, acceptor substrate
-
-
?
UDP-N-acetyl-D-galactosamine + fibronectin
UDP + N-acetyl-D-galactosaminyl-fibronectin
show the reaction diagram
-
fibronectin is a physiological substrate for GalNAc-T3
-
-
?
UDP-N-acetyl-D-galactosamine + GAGAPGPTPGPAGAGK
UDP + GAGAPGP-(N-acetyl-D-galactosaminylT)-PGPAGAGK
show the reaction diagram
-
optimal isozyme ppGalNAc T2 peptide substrate
-
-
?
UDP-N-acetyl-D-galactosamine + GAGPGTXASSVGVTGAPGSG
UDP + ?
show the reaction diagram
-
low activity
-
-
?
UDP-N-acetyl-D-galactosamine + GalNAc-glycosylated peptide
UDP + N-acetyl-D-galactosaminyl-GalNAc-glucosylated peptide
show the reaction diagram
UDP-N-acetyl-D-galactosamine + GKPRPYSPRPTSHPRPIRV
UDP + ?
show the reaction diagram
i.e. drosocin, low activity, enzyme form PGANT8
-
-
-
UDP-N-acetyl-D-galactosamine + GSPAGDVGAPAVDSGASQAAG
UDP + ?
show the reaction diagram
-
low activity
-
-
?
UDP-N-acetyl-D-galactosamine + GTTPSPVPTTG
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + GTTPSPVPTTS-(O-GalNAc)T-SAP
UDP + ?
show the reaction diagram
UDP-N-acetyl-D-galactosamine + GTTPSPVPTTSTTSA
UDP + N-acetyl-D-galactosaminyl-[GTTPSPVPTTSTTSA]
show the reaction diagram
-
i.e. Muc5Ac peptide
-
-
?
UDP-N-acetyl-D-galactosamine + GTTPSPVPTTSTTSAk
UDP + ?
show the reaction diagram
UDP-N-acetyl-D-galactosamine + GTTPSPVPTTSTTSAP
UDP + ?
show the reaction diagram
UDP-N-acetyl-D-galactosamine + HGVTSAPDTRP
UDP + ?
show the reaction diagram
UDP-N-acetyl-D-galactosamine + HIV-V3 peptide
UDP + N-acetyl-D-galactosaminyl-HIV-V3 peptide
show the reaction diagram
UDP-N-acetyl-D-galactosamine + human chorionic gonadotropin-beta peptide
UDP + N-acetyl-D-galactosaminyl-human chorionic gonadotropin-beta peptide
show the reaction diagram
UDP-N-acetyl-D-galactosamine + immunoglobulin A1
UDP + N-acetyl-D-galactosaminyl-immunoglobulin A1
show the reaction diagram
-
O-linked glycosylation of the human IgA1 hinge region by ppGalNAc-T2, synthetic IgA hinge peptide: ppGalNAc-T2 shows strongest activity and is able to transfer GalNAc to almost all possible glycosylation sites, other ppGalNAc-Ts show extremely weak activities
-
-
?
UDP-N-acetyl-D-galactosamine + ITTTTTPLPTG
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + kappa-casein
UDP + N-acetyl-D-galactosaminyl-kappa-casein
show the reaction diagram
UDP-N-acetyl-D-galactosamine + LGEPLGLAPSSSP
UDP + ?
show the reaction diagram
-
low activity
-
-
?
UDP-N-acetyl-D-galactosamine + LPPVTTTPAPG
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + MPTAGTPPPIG
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + mucin MUC4AC-13
UDP + ?
show the reaction diagram
enzyme form PGANT2
-
-
?
UDP-N-acetyl-D-galactosamine + mucin MUC5AC-3
UDP + ?
show the reaction diagram
UDP-N-acetyl-D-galactosamine + mucin MUC5AC-3/13
UDP + ?
show the reaction diagram
UDP-N-acetyl-D-galactosamine + mucin MUC5AX-13
UDP + ?
show the reaction diagram
UDP-N-acetyl-D-galactosamine + myelin basic protein
UDP + N-acetyl-D-galactosaminyl-myelin basic protein
show the reaction diagram
UDP-N-acetyl-D-galactosamine + osteopontin
UDP + N-acetyl-D-galactosaminyl-osteopontin
show the reaction diagram
UDP-N-acetyl-D-galactosamine + P-(O-GalNAc)T-TDSTTPAPTTK
UDP + ?
show the reaction diagram
UDP-N-acetyl-D-galactosamine + PADVTTAAPGG
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + PDTRPAPGSTAPPAC
UDP + ?
show the reaction diagram
UDP-N-acetyl-D-galactosamine + PGGDSSSYQPG
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + PGLPPTTPAVTTTATP
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + PHMAQVTVGPGL
UDP + N-acetyl-D-galactosaminyl-PHMAQVTVGPGL
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + PITTTTTVTPG
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
show the reaction diagram
UDP-N-acetyl-D-galactosamine + PPAHGVTSAPG
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + PPDAATAAPLR
UDP + N-acetyl-D-galactosaminyl-PPDAATAAPLR
show the reaction diagram
-
i.e. EPO-T peptide
-
-
?
UDP-N-acetyl-D-galactosamine + PPETTAAPPTG
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + PPVVPFTPLPG
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + PRFQDSSSSKAPPPSLPSPSRL
UDP + N-actetyl-D-galactosaminylated PRFQDSSSSKAPPPSLPSPSRL
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + PRFQDSSSSKAPPPSLPSPSRLPG
UDP + N-acetyl-D-galactosaminylated PRFQDSSSSKAPPPSLPSPSRLPG
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-galactosamine + PSPPITTTTTG
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + PSTPPTPSPSTPPTPSPS
UDP + ?
show the reaction diagram
UDP-N-acetyl-D-galactosamine + PTTDS-(O-GalNAc)T-TPAPTTK
UDP + ?
show the reaction diagram
UDP-N-acetyl-D-galactosamine + PTTDSTTPAPTT
UDP + ?
show the reaction diagram
UDP-N-acetyl-D-galactosamine + PTTDSTTPAPTTK
UDP + ?
show the reaction diagram
UDP-N-acetyl-D-galactosamine + PTTTPISTTMVTPTPTPTC
UDP + N-actetyl-D-galactosaminylated PTTTPISTTMVTPTPTPTC
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + PTTTPISTTTMVTPTPTPTC
UDP + N-acetyl-D-galactosaminylated PTTTPISTTTMVTPTPTPTC
show the reaction diagram
mucin derived substrate
-
-
?
UDP-N-acetyl-D-galactosamine + PTTTPITTTTTV
UDP + ?
show the reaction diagram
UDP-N-acetyl-D-galactosamine + PTTTPITTTTTVTPTPTPTGTQT
UDP + ?
show the reaction diagram
UDP-N-acetyl-D-galactosamine + PTTTPSPPITG
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + QATEYEYLDYDFLPEC
UDP + N-acetyl-D-galactosaminylated QATEYEYLDYDFLPEC
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-galactosamine + rhodanese
UDP + N-acetyl-D-galactosaminyl-rhodanese
show the reaction diagram
-
reduced and carboxylmethylated bovine rhodanese, at a lower rate than with myelin basic protein
-
-
?
UDP-N-acetyl-D-galactosamine + RPAPGSTAPPA
UDP + N-acetyl-D-galactosaminylated RPAPGSTAPPA
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-galactosamine + SATTPAPPSSG
UDP + ?
show the reaction diagram
-
preferred substrate
-
-
?
UDP-N-acetyl-D-galactosamine + SDAESSGPVPG
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + SEVTTTLVPAG
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + SPTTSTPISSTPQPTS
UDP + ?
show the reaction diagram
UDP-N-acetyl-D-galactosamine + STPSTPSTPSTPSTP
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + subtilisin
UDP + N-acetyl-D-galactosaminyl-subtilisin
show the reaction diagram
-
reduced and carboxylmethylated bacterial subtilisin, at a lower rate than with myelin basic protein or bovine rhodanese
-
-
?
UDP-N-acetyl-D-galactosamine + SVLVLTTVSVPPSPASAGPSV
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + syndecan-3
UDP + N-acetyl-D-galactosaminyl-syndecan-3
show the reaction diagram
syndecan-3 may be a natural substrate for GalNAc-T13
-
-
?
UDP-N-acetyl-D-galactosamine + TAPPAHGVTSAPDTRPAPGSTAPP
UDP + N-acetyl-D-galactosaminylated TAPPAHGVTSAPDTRPAPGSTAPP
show the reaction diagram
UDP-N-acetyl-D-galactosamine + TSAPGTTPSPG
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + TTAAPPTPSAG
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + TTPLPTTTPSG
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + TTSTTSAPGTG
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + TTTTTTPKPTG
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + TTTVTPTPTPG
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + TVPVTGPTAPALVQSVTPPT
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + VESDRSTTTTQAP
UDP + ?
show the reaction diagram
UDP-N-acetyl-D-galactosamine + VLTTTATTPTA
UDP + ?
show the reaction diagram
UDP-N-acetyl-D-galactosamine + VPETASSKTATGDSFS
UDP + ?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-galactosamine + bone sialoprotein
UDP + UDP-N-acetyl-D-galactosaminyl-bone sialoprotein
show the reaction diagram
-
preferred substrate of isozyme ppGalNAcT-1, glycosylation of Thr101, Ser131, Thr199, and Ser214, glycosylation pattern, overview
-
-
?
UDP-N-acetyl-D-galactosamine + fibronectin
UDP + N-acetyl-D-galactosaminyl-fibronectin
show the reaction diagram
-
fibronectin is a physiological substrate for GalNAc-T3
-
-
?
UDP-N-acetyl-D-galactosamine + kappa-casein
UDP + N-acetyl-D-galactosaminyl-kappa-casein
show the reaction diagram
-
glycosylation of kappa-casein occurs after casein micelle formation triggered by the accumulation of Ca2+ in vivo
-
-
?
UDP-N-acetyl-D-galactosamine + osteopontin
UDP + N-acetyl-D-galactosaminyl-osteopontin
show the reaction diagram
-
preferred substrate of isozyme ppGalNAcT-1, glycosylation pattern, overview
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
show the reaction diagram
UDP-N-acetyl-D-galactosamine + syndecan-3
UDP + N-acetyl-D-galactosaminyl-syndecan-3
show the reaction diagram
Q8IUC8
syndecan-3 may be a natural substrate for GalNAc-T13
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ba2+
-
stimulates to a smaller extent than Mn2+
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ADP
-
weak
alphaS1-casein
-
strong inhibition of GalNAc transfer to kappa-casein, reversed by Ca2+
-
AMP
-
-
ATP
-
weak
benzyl-alpha-GalNAc
;
benzyl-N-acetyl-alpha-D-galactosaminide
-
benzyl-GalNAc, 2 mM over night in Hek293T cell culture, abolishes activity of transiently transfected xGalntl-1 in Hek293T cells like inhibition of Smad2 phosphorylation by ActR-IIB and induction of a migration shift of ActR-IIB and Alk-3
CDP
-
-
CMP
-
-
CTP
-
-
diethyldicarbonate
-
binds to His; hydroxylamine partiallly protects, UDP-GalNAc protects
fully GalNAc-substituted MUC1 repeat peptide
-
inhibits activity of rGalNAc-T4 and -T2
-
GalNAc
GDP
-
weak
GMP
-
-
GTP
-
-
Helix pomatia lectin
-
-
-
HIV-V3
-
competitive inhibition of Muc2 glycosylation
-
methyl-alpha-Gal
-
Muc2
-
competitive inhibition of HIV-V3 peptide glycosylation
-
o-nitrophenyl-alpha-GalNAc
;
o-nitrophenyl-beta-GalNAc
;
p-Chloromercuriphenylsulfonic acid
-
irreversible
p-nitrophenyl-alpha-GalNAc
;
p-nitrophenyl-beta-GalNAc
;
phenyl-alpha-GalNAc
;
Soybean lectin
-
-
-
UDP-alpha-GalNAc
;
UMP-1-hexadecanesulfonic anhydride
-
50% inhibition at 0.16 mM, competitive
UMP-1-octanesulfonic anhydride
-
-
UMP-butanesulfonic anhydride
-
-
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Triton X-100
additional information
GalNAc-T7: selectively activated by partial GalNAc glycosylation of peptide substrates derived from the tandem repeats of human Muc2 and rat submaxillary gland mucin
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2
Ac-Asn-Leu-Thr-Pro-Pro-Pro
-
-
1.3
Ac-Pro-Thr-Thr-Thr
1.33
Ac-Thr-Pro-Pro-Pro
-
-
3.7
Ac-TPPA
-
-
0.45 - 0.91
AHGVVTSAPDTR
0.24
AQPTPPP
-
-
0.4 - 3.3
Arg-Thr-Pro-Pro-Pro
14.9
Asp-Ala-Ala-Ser-Ala-Ala-Pro-Leu
-
-
0.01 - 0.48
CPPTPSATTPAPPSSSAPPETTAA
0.0175
deglycosylated human trachea mucin
-
-
-
0.0141
deglycosylated swine trachea mucin
-
-
-
0.07 - 2.19
DSTTPAPTTK
0.595
EA2 peptide
-
-
39.5
Gln-Ala-Ala-Gly-Thr-Ser-Gly-Ala-Gly-Pro-Gly
-
-
0.8
Gly-Val-Val-Pro-Thr-Val-Val-Pro-Gly
-
GalNAc-T1
0.0209 - 0.0721
GTTPSPVPTTS-(O-GalNAc)T-SAP
0.0259 - 0.344
GTTPSPVPTTSTTSAP
0.115 - 0.46
GTTPSPVPTTST[GalNAc]TSAP
0.035 - 0.332
GT[GalNAc]TPSPVPTTSTTSAP
0.206
GT[GalNAc]TPSPVPTTST[GalNAc]TSAP
-
pH not specified in the publication, 37C
1.74 - 17.6
GVVPTVVPG
0.5
HIV-V3 peptide
-
-
-
0.23
human mucin Muc2 peptide
-
-
-
0.01 - 0.02
IgA hinge
0.12 - 0.81
IgA hinge-4GalNAc
0.15 - 0.36
MUC1
0.86 - 0.87
MUC1-2GalNAc
0.07 - 0.23
Muc1a' peptide
0.01 - 0.25
Muc2
0.062 - 0.066
MUC2-6GalNAc
0.018 - 0.02
MUC5AC
0.17 - 0.77
MUC5AC-13
0.033 - 0.107
MUC5AC-3
0.44 - 1
MUC5AC-3,13
2.4
MUC5AC-9
-
at site Thr-2, hT10
0.02 - 0.054
Muc7 peptide
0.4
myelin basic protein
-
-
-
0.25 - 0.3
N-acetyl-4-benzoyl-L-Phe-L-Pro-L-Tyr-L-Pro-L-Pro-L-Pro-NH2
0.35 - 15.6
PPDAATAAPL
6 - 6.5
Pro-Pro-Ala-Ser-Thr-Ser-Ala-Pro-Gly
3.6 - 4.5
Pro-Pro-Asp-Ala-Ala-Ser-Ala-Ala-Pro-Leu-Arg
0.2
Pro-Pro-Asp-Ala-Ala-Thr-Ala-Ala-Pro-Leu
-
GalNAc-T1
-
18
Pro-Pro-Asp-Ala-Ser-Ser-Ser-Ala-Pro-Leu-Arg
-
-
12.9
Pro-Pro-Asp-Val-Val-Ser-Val-Val-Pro-Leu-Arg
-
-
0.52
Pro-Thr-Ala-Pro-Pro-Pro
-
-
0.1058 - 0.198
PTTDS-(O-GalNAc)T-TPAPTTK
0.042 - 1.02
PTTDSTTPAPTTK
1.33
SDC106 peptide
derived from human syndecan-3, ppGalNac-T13
-
0.63
SDC155
derived from human syndecan-3, ppGalNac-T13
-
0.38
SDC165 peptide
derived from human syndecan-3, ppGalNac-T13
-
0.07 - 0.96
SDC284 peptide
0.2 - 0.65
STPSTPSTPSTPSTP
0.027 - 0.041
UDP-Gal
0.0017 - 16
UDP-GalNAc
0.008 - 0.081
UDP-N-acetyl-D-galactosamine
14
Val-Leu-Gly-Ala-Thr-Ala-Val
-
-
8.3
Val-Leu-Gly-Thr-Ala-Ala-Val
-
-
18.6
Val-Leu-Gly-Thr-Thr-Ala-Val
-
-
7.2
Val-Lys-Thr-Glu-Ala-Thr-Thr-Phe-Ile
-
-
3
Val-Thr-Pro-Arg-Thr-Pro-Pro-Pro
-
-
additional information
apomucin