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Literature summary for 2.4.1.41 extracted from

  • Zhang, Y.; Iwasaki, H.; Wang, H.; Kudo, T.; Kalka, T.B.; Hennet, T.; Kubota, T.; Cheng, L.; Inaba, N.; Gotoh, M.; Togayachi, A.; Guo, J.; Hisatomi, H.; Nakajima, K.; Nishihara, S.; Nakamura, M.; Marth, J.D.; Narimatsu, H.
    Cloning and characterization of a new human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase, designated pp-GalNAc-T13, that is specifically expressed in neurons and synthesizes GalNAc alpha-serine/threonine antigen (2003), J. Biol. Chem., 278, 573-584.
    View publication on PubMed
Search for more literature for 2.4.1.41

Cloned(Commentary)

Cloned (Comment) Organism
-
 Homo sapiens
ppGalNAc-T13 and -T1 are localized to chromosome 2 and 18, ppGalNAc-T13 is cloned and sequenced, 556-amino acid protein Mus musculus
ppGalNAc-T13, -T1 and -T9 are cloned, and expressed in Sf21 or High FiveTM insect cells in a soluble form, ppGalNAc-T13: gene is localized to chromosome 2, nucleotide sequence encodes a 556-amino acid protein, ppGalNAc-T1 is localized to chromosome 18 Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information ppGalNAc-T13 deficient mice with decrease in Tn antigen expression in the cerebellum Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 Muc7 peptide ppGalNac-T9 Homo sapiens
0.046
-
 Muc7 peptide ppGalNac-T13 Homo sapiens
0.054
-
 Muc7 peptide ppGalNac-T1 Homo sapiens
0.07
-
 Muc1a' peptide ppGalNac-T9 Homo sapiens
0.07
-
 SDC284 peptide derived from human syndecan-3, ppGalNac-T13 Homo sapiens
0.11
-
 Muc1a' peptide ppGalNac-T13 Homo sapiens
0.19
-
 SDC284 peptide derived from human syndecan-3, ppGalNac-T1 Homo sapiens
0.23
-
 Muc1a' peptide ppGalNac-T1 Homo sapiens
0.38
-
 SDC165 peptide derived from human syndecan-3, ppGalNac-T13 Homo sapiens
0.63
-
 SDC155 peptide derived from human syndecan-3, ppGalNac-T13 Homo sapiens
0.96
-
 SDC284 peptide derived from human syndecan-3, ppGalNac-T9 Homo sapiens
1.33
-
 SDC106 peptide derived from human syndecan-3, ppGalNac-T13 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Mus musculus 16020
-
membrane ppGaNTase-T13: type II membrane protein with a 20-amino acid transmembrane segment and an 8-amino acid N-terminal cytoplasmic domain Homo sapiens 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens pp-GalNAc-T13 is specifically expressed in neurons and synthesizes Tn antigen ?
-
 ?
UDP-N-acetyl-D-galactosamine + polypeptide Mus musculus
-
 UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?
UDP-N-acetyl-D-galactosamine + polypeptide Homo sapiens ppGalNAc-T13: synthesis of O-glycan, specifically the Tn antigen in neurons UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?
UDP-N-acetyl-D-galactosamine + syndecan-3 Homo sapiens syndecan-3 may be a natural substrate for GalNAc-T13 UDP + N-acetyl-D-galactosaminyl-syndecan-3
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 ppGalNAc-T13, -T1 and -T9
-
Homo sapiens Q8IUC8
-
-
Mus musculus
-
 ppGalNAc-T13, subsequently termed ppGalNAc-T8
-

Purification (Commentary)

Purification (Comment) Organism
ppGalNAc-T13, -T1 and -T9, expressed in Sf21 or High FiveTM insect cells Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain ppGalNAc-T13 expression only in brain, exclusively in neuronal cells Mus musculus
-
brain ppGalNAc-T13 is highly and restrictively expressed in brain, fetal brain, adult cerebellum and cerebral cortex, exclusively in neuronal cells Homo sapiens
-
brain pp-GalNAc-T13 is specifically expressed in neurons Homo sapiens
-
additional information ppGalNAc-T13: not in astrocytes Mus musculus
-
additional information tissue distribution of several ppGalNAc-transferases Mus musculus
-
additional information tissue distribution of several ppGalNAc-transferases Homo sapiens
-
additional information ppGalNAc-T1 is ubiquitously expressed, ppGalNAc-T13: not in glioblastoma cells and primary cultured astrocytes Homo sapiens
-
neuroblastoma cell ppGalNAc-T13 is abundantly expressed in neuroblastoma cells Homo sapiens
-
neuron ppGalNAc-T13 is abundantly expressed in primary cultured neurons Homo sapiens
-
neuron pp-GalNAc-T13 is specifically expressed in neurons Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information multiple enzyme isoforms Mus musculus ?
-
 ?
additional information multiple enzyme isoforms Homo sapiens ?
-
 ?
additional information pp-GalNAc-T13 is specifically expressed in neurons and synthesizes Tn antigen Homo sapiens ?
-
 ?
UDP-N-acetyl-alpha-D-galactosamine + Muc7 peptide
-
 Homo sapiens ?
-
 ?
UDP-N-acetyl-alpha-D-galactosamine + SDC106 peptide
-
 Homo sapiens ?
-
 ?
UDP-N-acetyl-alpha-D-galactosamine + SDC155 peptide
-
 Homo sapiens ?
-
 ?
UDP-N-acetyl-alpha-D-galactosamine + SDC165 peptide
-
 Homo sapiens ?
-
 ?
UDP-N-acetyl-alpha-D-galactosamine + SDC284 peptide
-
 Homo sapiens ?
-
 ?
UDP-N-acetyl-D-galactosamine + polypeptide
-
 Mus musculus UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?
UDP-N-acetyl-D-galactosamine + polypeptide ppGalNAc-T13: acceptors are mucin peptides, such as Muc1a', Muc5Ac and Muc7, forms a triplet Tn epitope, 3 consecutive GalNAc-Ser/Thr structures, on peptides encoded in syndecan-3, different substrate specificities of ppGalNAc-T1 and -T13 Homo sapiens UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?
UDP-N-acetyl-D-galactosamine + polypeptide ppGalNAc-T13: synthesis of O-glycan, specifically the Tn antigen in neurons Homo sapiens UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?
UDP-N-acetyl-D-galactosamine + syndecan-3 syndecan-3 may be a natural substrate for GalNAc-T13 Homo sapiens UDP + N-acetyl-D-galactosaminyl-syndecan-3
-
 ?

Synonyms

Synonyms Comment Organism
pp-GalNAc-T13
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus
37
-
 assay at Homo sapiens