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Literature summary for 2.4.1.41 extracted from
- Prokaryotic expression, purification, and production of polyclonal antibody against human polypeptide N-acetylgalactosaminyltransferase 14 (2007), Protein Expr. Purif., 56, 1-7.
Application
| Application | Comment | Organism |
|---|---|---|
| molecular biology | polyclonal rabbit anti-GalNAc-T14 IgG (1: 1000 in Western blot, titer: appr. 1: 16000) for study of expression and distribution of human GalNAc-T14 | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells and HEK-293T cells | Homo sapiens |
| full-length GalNAc-T14 in pFlag-CMV2, expression in HEK293T cells | Homo sapiens |
| full-length GalNAc-T14 in pGEX-4T-1, expression in Escherichia coli BL21(DE3) | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| Golgi apparatus | - |
Homo sapiens | 5794 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 61000 | - |
endogenous GalNAc-T14 in HEK293T cells detected by anti-GalNAc-T14 antibody | Homo sapiens |
| 61000 | - |
Flag-tag fusion protein expressed in HEK293T cells detected by anti-Flag and anti-GalNAc-T14 antibody | Homo sapiens |
| 87000 | - |
SDS-PAGE | Homo sapiens |
| 87000 | - |
bacterially expressed GST-fusion protein (GST: 26 kDa) detected by anti-GST and anti-GalNAc-T14 antibody | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-D-galactosamine + polypeptide | Homo sapiens | mucin-type O-linked glycosylation of serine or threonine residues | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q96FL9 | - |
- |
| Homo sapiens | Q96FL9 | GalNAc-T14 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| GST fusion protein purified by affinity chromatography, elution with 10 mM glutathione, pH8 | Homo sapiens |
| GSTrap FF chromatography | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Homo sapiens | - |
| brain | cDNA library used for cloning | Homo sapiens | - |
| HEK-293T cell | expression detected by Western blot | Homo sapiens | - |
| MCF-7 cell | no expression detected by Western blot | Homo sapiens | - |
| renal cell carcinoma cell | RCC 7860, expression detected by Western blot | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-D-galactosamine + polypeptide | - |
Homo sapiens | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
| UDP-N-acetyl-D-galactosamine + polypeptide | mucin-type O-linked glycosylation of serine or threonine residues | Homo sapiens | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GalNAc-T | - |
Homo sapiens |
| GalNAc-T14 | - |
Homo sapiens |
| polypeptide N-acetylgalactosaminyltransferase | - |
Homo sapiens |
| polypeptide N-acetylgalactosaminyltransferase 14 | - |
Homo sapiens |
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