Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DTT | required for maximal activity | Bos taurus | |
Triton X-100 | required for maximal activity | Bos taurus |
Application | Comment | Organism |
---|---|---|
molecular biology | enzyme will be useful for the in vitro glycosylation of proteins obtained from microorganisms by gene manipulation techniques | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | weak | Bos taurus | |
alphaS1-casein | strong inhibition of GalNAc transfer to kappa-casein, reversed by Ca2+ | Bos taurus | |
AMP | - |
Bos taurus | |
ATP | weak | Bos taurus | |
CDP | - |
Bos taurus | |
CMP | - |
Bos taurus | |
CTP | - |
Bos taurus | |
GDP | weak | Bos taurus | |
GMP | - |
Bos taurus | |
GTP | - |
Bos taurus | |
Helix pomatia lectin | - |
Bos taurus | |
additional information | not inhibited by wheat germ lectin | Bos taurus | |
Soybean lectin | - |
Bos taurus | |
UDP | strong | Bos taurus | |
UMP | - |
Bos taurus | |
UTP | - |
Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km: 0.192 mg/ml: apomucin, 1.15 mg/ml: kappa-subcomponent 1, 5.1 mg/ml: kappa-subcomponent 7 | Bos taurus | |
0.0162 | - |
UDP-GalNAc | - |
Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi membrane | specifically localized in | Bos taurus | 139 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | stimulates to a smaller extent than Mn2+ | Bos taurus | |
Ca2+ | stimulates to a smaller extent than Mn2+ | Bos taurus | |
Mg2+ | stimulates to a smaller extent than Mn2+ | Bos taurus | |
Mn2+ | requirement | Bos taurus | |
Mn2+ | strong stimulation, maximal activity at 4-10 mM | Bos taurus | |
Zn2+ | stimulates to a smaller extent than Mn2+ | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
200000 | - |
gel filtration | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-galactosamine + kappa-casein | Bos taurus | glycosylation of kappa-casein occurs after casein micelle formation triggered by the accumulation of Ca2+ in vivo | UDP + N-acetyl-D-galactosaminyl-kappa-casein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
lactating cow | - |
Purification (Comment) | Organism |
---|---|
7500fold | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mammary gland | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00863 | - |
- |
Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | not: alphaS1-casein, alphaS2-casein, beta-casein, alpha-lactalbumin, beta-lactoglobulin, bovine serum albumin | Bos taurus | ? | - |
? | |
UDP-N-acetyl-D-galactosamine + apomucin | best substrate, poorer substrates: asialo mucin, native mucin | Bos taurus | UDP + N-acetyl-D-galactosaminyl-apomucin | - |
? | |
UDP-N-acetyl-D-galactosamine + kappa-casein | kappa-subcomponent 1, the nonglycosylated kappa-casein, is the best substrate among kappa-casein subcomponents, asialo kappa-casein, agalacto kappa-casein and nature kappa-casein | Bos taurus | UDP + N-acetyl-D-galactosaminyl-kappa-casein | - |
? | |
UDP-N-acetyl-D-galactosamine + kappa-casein | glycosylation of kappa-casein occurs after casein micelle formation triggered by the accumulation of Ca2+ in vivo | Bos taurus | UDP + N-acetyl-D-galactosaminyl-kappa-casein | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | acceptor: asialo mucin, subcomponents of kappa-casein, asialo kappa-casein, agalacto kappa-casein | Bos taurus | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | acceptors: apomucin, kappa-casein | Bos taurus | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Bos taurus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 35 | 1 h, less than 20% loss of activity | Bos taurus |
45 | - |
1 h, 50% loss of activity | Bos taurus |
55 | - |
1 h, complete loss of activity | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 6.4 | Bos taurus |
6.5 | 7.5 | Tris, imidazole and MES buffer, kappa-casein | Bos taurus |