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Literature summary for 2.4.1.41 extracted from

  • Freire, T.; Fernandez, C.; Chalar, C.; Maizels, R.M.; Alzari, P.; Osinaga, E.; Robello, C.
    Characterization of a UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase with an unusual lectin domain from the platyhelminth parasite Echinococcus granulosus (2004), Biochem. J., 382, 501-510.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.41

Cloned(Commentary)

Cloned (Comment) Organism
expression in COS-7 cells Echinococcus granulosus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 10 mM, 5.4fold activation Echinococcus granulosus
Co2+ 10 mM, 5.9fold activation Echinococcus granulosus
Cu2+ 10 mM, 11.4fold activation, recombinant protein lacking the trans-membrane domain shows maximal activity in presence of Cu2+ Echinococcus granulosus
Mg2+ 10 mM, 6.4fold activation Echinococcus granulosus
Mn2+ 10 mM, 7fold activation Echinococcus granulosus
Ni2+ 10 mM, 6.1fold activation Echinococcus granulosus
Zn2+ 10 mM, 5.7fold activation Echinococcus granulosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-D-galactosamine + polypeptide Echinococcus granulosus can participate in the biosynthesis of O-glycosylated parasite proteins exposed at the interface between Echinococcus granulosus and its hosts UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?

Organism

Organism UniProt Comment Textmining
Echinococcus granulosus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cyst hydatid cyst wall Echinococcus granulosus
-
larva subtegumental region of larval worms Echinococcus granulosus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-D-galactosamine + deglycosylated ovine submaxillary mucin
-
 Echinococcus granulosus UDP + ?
-
 ?
UDP-N-acetyl-D-galactosamine + polypeptide can participate in the biosynthesis of O-glycosylated parasite proteins exposed at the interface between Echinococcus granulosus and its hosts Echinococcus granulosus UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?
UDP-N-acetyl-D-galactosamine + polypeptide transfers GalNAc to a broad range of substrate peptides, derived from human mucins and O-glycosylated parasite proteins, including acceptors containing only serine or only threonine residues Echinococcus granulosus UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?

Synonyms

Synonyms Comment Organism
Eg-ppGalNAc-T1
-
 Echinococcus granulosus
ppGalNAc-T
-
 Echinococcus granulosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
 recombinant protein lacking the trans-membrane domain Echinococcus granulosus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 37 20°C: 49% of maximal activity, 37°C: 48% of maximal activity Echinococcus granulosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 recombinant protein lacking the trans-membrane domain Echinococcus granulosus

pH Range

pH Minimum pH Maximum Comment Organism
6 8 about 60% of maximal activity at pH 6.0 and at pH 8.0 Echinococcus granulosus