Cloned (Comment) | Organism |
---|---|
gene encoding ppGaNTase-T9 from sublingual gland is cloned, characterized, sequenced and expressed transiently in COS7 cells, gene encodes a unique 603-amino acid protein | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Mus musculus | 16020 | - |
membrane | ppGaNTase-T9: type II integral membrane protein with a 20-amino acid transmembrane domain, a 9-amino acid cytoplasmic domain and a 94-amino acid stem region | Rattus norvegicus | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-galactosamine + polypeptide | Rattus norvegicus | diversely regulated ppGaNTase family may play a role in the various processes governing development | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | Mus musculus | initiation of mucin-type O-glycosylation by a family of polypeptide GalNAc-transferases, of which each has a unique function | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | Rattus norvegicus | initiation of mucin-type O-glycosylation by a family of polypeptide GalNAc-transferases, of which each has a unique function | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Rattus norvegicus | Q925R7 | ppGaNTase-T9 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Mus musculus | - |
brain | ppGaNTase-T9, less amount | Rattus norvegicus | - |
colon | - |
Mus musculus | - |
colon | ppGaNTase-T9 | Rattus norvegicus | - |
embryo | embryonic day 14.5, ppGaNTase-T9 is expressed relatively abundantly compared with ppGaNTase-T7, accumulation in brain | Mus musculus | - |
heart | - |
Mus musculus | - |
heart | ppGaNTase-T9, less amount | Rattus norvegicus | - |
lung | - |
Mus musculus | - |
lung | ppGaNTase-T9, less amount | Rattus norvegicus | - |
ovary | - |
Mus musculus | - |
ovary | ppGaNTase-T9 | Rattus norvegicus | - |
small intestine | - |
Mus musculus | - |
small intestine | ppGaNTase-T9 | Rattus norvegicus | - |
spleen | - |
Mus musculus | - |
spleen | ppGaNTase-T9, less amount | Rattus norvegicus | - |
stomach | - |
Mus musculus | - |
stomach | ppGaNTase-T9, less amount | Rattus norvegicus | - |
sublingual gland | - |
Mus musculus | - |
sublingual gland | ppGaNTase-T9 | Rattus norvegicus | - |
testis | ppGaNTase-T9 | Rattus norvegicus | - |
uterine cervix | - |
Mus musculus | - |
uterine cervix | ppGaNTase-T9, less amount | Rattus norvegicus | - |
uterus | - |
Mus musculus | - |
uterus | ppGaNTase-T9, less amount | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | multiple enzyme isoforms | Mus musculus | ? | - |
? | |
additional information | multiple enzyme isoforms | Rattus norvegicus | ? | - |
? | |
additional information | 9 isoforms, ppGaNTase-T9 contains a 480-amino acid conserved region and shows no activity with non-glycosylated peptides | Rattus norvegicus | ? | - |
? | |
UDP-N-acetyl-D-galactosamine + GalNAc-glycosylated peptide | ppGaNTase-T9: acceptors are di- and triglycosylated MUC5AC and mono-glycosylated EA2, obtained by prior action of ppGaNTase-T1, but not the unmodified peptides, formation of a tetra-glycopeptide species from MUC5AC triglycopeptide substrate, ppGaNTase-T7 forms a hexa-glycopeptide species | Rattus norvegicus | UDP + N-acetyl-D-galactosaminyl-GalNAc-glucosylated peptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | - |
Mus musculus | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | transfer of GalNAc to a serine or threonine residue on the acceptor protein | Rattus norvegicus | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | diversely regulated ppGaNTase family may play a role in the various processes governing development | Rattus norvegicus | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | initiation of mucin-type O-glycosylation by a family of polypeptide GalNAc-transferases, of which each has a unique function | Mus musculus | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | initiation of mucin-type O-glycosylation by a family of polypeptide GalNAc-transferases, of which each has a unique function | Rattus norvegicus | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
37 | - |
assay at | Rattus norvegicus |