Information on EC 2.4.2.30 - NAD+ ADP-ribosyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.2.30
-
RECOMMENDED NAME
GeneOntology No.
NAD+ ADP-ribosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
NAD+ + (ADP-D-ribosyl)n-acceptor = nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pentosyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
NAD metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
NAD+:poly(ADP-D-ribosyl)-acceptor ADP-D-ribosyl-transferase
The ADP-D-ribosyl group of NAD+ is transferred to an acceptor carboxy group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20--30 units.
CAS REGISTRY NUMBER
COMMENTARY hide
58319-92-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
strain 2339
-
-
Manually annotated by BRENDA team
strain 2339
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
type c
-
-
Manually annotated by BRENDA team
Clostridium limosum
-
-
-
Manually annotated by BRENDA team
Cryptothecodinium cohnii
-
-
-
Manually annotated by BRENDA team
ModA; ModB
Uniprot
Manually annotated by BRENDA team
Enterobacteria phage T4 ModA
ModA
Uniprot
Manually annotated by BRENDA team
Enterobacteria phage T4 ModB
ModB
Uniprot
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Mus musculus 129/Sv x C57BL/6
strain 129/Sv x C57BL/6, female mice
-
-
Manually annotated by BRENDA team
Sv129 mice
-
-
Manually annotated by BRENDA team
ExoS
-
-
Manually annotated by BRENDA team
ExoT
-
-
Manually annotated by BRENDA team
strain PA103
-
-
Manually annotated by BRENDA team
recombinant enzyme
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
disruption of PARP1 enzymatic activity causes nucleolar disintegration and aberrant localization of nucleolar-specific proteins. PARP1 mutants have increased accumulation of rRNA intermediates and a decrease in ribosome levels
metabolism
-
the lowest PARP activity, as well as the lowest quantum yield of PSII linear electron transport (FPSII) and photochemical quenching (qP), is found in outdoor during winter plants. In outdoor spring plants the recovery of photochemical activity associated to a poly(ADP-ribose)polymerase activity increase of about 50%, as compared to greenhouse plants, is observed
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
integrin alpha7 + NAD+
(ADP-D-ribosyl)-integrin alpha7 + nicotinamide
show the reaction diagram
NAD+ + (ADP-D-ribosyl)n-(EF-2)
nicotinamide + (ADP-D-ribosyl)n+1-(EF-2)
show the reaction diagram
-
in native conformation, CRM66 shows limited ability to modify EF-2 covalently. Upon activation with urea and dithiothreitol CRM66 loses ADP-ribosylation activity entirely, yet it retains the ability to bind NAD+. Replacement of Tyr-426 with histidine in CRM66 completely restores cytotoxicity and ADP-ribosyltransferase activity
-
-
?
NAD+ + (ADP-D-ribosyl)n-acceptor
nicotinamide + (ADP-D-ribosyl)n+1-acceptor
show the reaction diagram
NAD+ + (ADP-D-ribosyl)n-acceptor
nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H+
show the reaction diagram
NAD+ + (ADP-D-ribosyl)n-actin
nicotinamide + (ADP-D-ribosyl)n+1-actin
show the reaction diagram
VahC is shown to ADP-ribosylate Arg-177 of actin. VahC activity causes depolymerization of actin filaments, which induces caspase-mediated apoptosis in HeLa Tet-Off cells
-
-
?
NAD+ + (ADP-D-ribosyl)n-apolipoprotein
nicotinamide + (ADP-D-ribosyl)n+1-apolipoprotein
show the reaction diagram
-
-
-
-
?
NAD+ + (ADP-D-ribosyl)n-Crk-I
nicotinamide + (ADP-D-ribosyl)n+1-Crk-I
show the reaction diagram
-
-
-
-
?
NAD+ + (ADP-D-ribosyl)n-Crk-II
nicotinamide + (ADP-D-ribosyl)n+1-Crk-II
show the reaction diagram
-
-
-
-
?
NAD+ + (ADP-D-ribosyl)n-His6HRas
nicotinamide + (ADP-D-ribosyl)n+1-His6HRas
show the reaction diagram
-
the enzyme preferentially ADP-ribosylates membrane-associated His6HRas relative to its cytosolic His6HRasDELTACAAX with a C-terminal deletion
-
-
?
NAD+ + (ADP-D-ribosyl)n-immunoglobulin A
nicotinamide + (ADP-D-ribosyl)n+1-immunoglobulin A
show the reaction diagram
-
-
-
-
?
NAD+ + (ADP-D-ribosyl)n-immunoglobulin G
nicotinamide + (ADP-D-ribosyl)n+1-immunoglobulin G
show the reaction diagram
-
preferentially IgG3
-
-
?
NAD+ + (ADP-D-ribosyl)n-p21ras
nicotinamide + (ADP-D-ribosyl)n+1-p21ras
show the reaction diagram
NAD+ + (ADP-D-ribosyl)n-Rab2
nicotinamide + (ADP-D-ribosyl)n+1-Rab2
show the reaction diagram
-
-
-
-
?
NAD+ + (ADP-D-ribosyl)n-Rab3
nicotinamide + (ADP-D-ribosyl)n+1-Rab3
show the reaction diagram
-
-
-
-
?
NAD+ + (ADP-D-ribosyl)n-rab4
nicotinamide + (ADP-D-ribosyl)n+1-rab4
show the reaction diagram
NAD+ + (ADP-D-ribosyl)n-Rab5
nicotinamide + (ADP-D-ribosyl)n+1-Rab5
show the reaction diagram
NAD+ + (ADP-D-ribosyl)n-RalA
nicotinamide + (ADP-D-ribosyl)n+1-RalA
show the reaction diagram
NAD+ + (ADP-D-ribosyl)n-Rap1A
nicotinamide + (ADP-D-ribosyl)n+1-Rap1A
show the reaction diagram
-
-
-
-
?
NAD+ + (ADP-D-ribosyl)n-Ras
nicotinamide + (ADP-D-ribosyl)n+1-Ras
show the reaction diagram
NAD+ + (ADP-D-ribosyl)n-Ras protein
nicotinamide + (ADP-D-ribosyl)n+1-Ras protein
show the reaction diagram
-
-
-
-
?
NAD+ + (ADP-D-ribosyl)n-soybean trypsin inhibitor
nicotinamide + (ADP-D-ribosyl)n+1-soybean trypsin inhibitor
show the reaction diagram
-
-
-
-
?
NAD+ + (ADP-D-ribosyl)n-soybean-trypsin-inhibitor
nicotinamide + (ADP-D-ribosyl)n+1-soybean-trypsin-inhibitor
show the reaction diagram
-
-
-
-
?
NAD+ + cyclophilin A
nicotinamide + (ADP-D-ribosyl)-cyclophilin A
show the reaction diagram
NAD+ + GTPase RhoA
nicotinamide + (ADP-D-ribosyl)-GTPase RhoA
show the reaction diagram
Clostridium limosum
-
the exoenzyme modifies the low-molecular-mass GTPases RhoA, B, and C specifically at Asn41
-
-
?
NAD+ + GTPase RhoB
nicotinamide + (ADP-D-ribosyl)-GTPase RhoB
show the reaction diagram
Clostridium limosum
-
the exoenzyme modifies the low-molecular-mass GTPases RhoA, B, and C specifically at Asn41
-
-
?
NAD+ + GTPase RhoC
nicotinamide + (ADP-D-ribosyl)-GTPase RhoC
show the reaction diagram
Clostridium limosum
-
the exoenzyme modifies the low-molecular-mass GTPases RhoA, B, and C specifically at Asn41
-
-
?
NAD+ + H2O
nicotinamide + ADP-ribose
show the reaction diagram
-
-
-
-
?
NAD+ + histone H1
nicotinamide + (ADP-D-ribosyl)-histone H1
show the reaction diagram
-
-
-
-
?
NAD+ + histone H2A
nicotinamide + (ADP-D-ribosyl)-histone H2A
show the reaction diagram
NAD+ + histone H2B
nicotinamide + (ADP-D-ribosyl)-histone H2B
show the reaction diagram
NAD+ + lymphocyte function-associated antigen LFA-1
nicotinamide + ?
show the reaction diagram
-
-
-
-
?
NAD+ + moesin
nicotinamide + (ADP-D-ribosyl)-moesin
show the reaction diagram
NAD+ + p53
nicotinamide + (ADP-D-ribosyl)-p53
show the reaction diagram
NAD+ + poly(ADP-ribose) polymerase-1
nicotinamide + (ADP-ribosyl)-poly(ADP-ribose) polymerase-1
show the reaction diagram
-
the 40 kDa CD fragment of avian PARP-1 efficiently catalyzes a covalent auto-poly-(ADP-ribosyl)ation reaction via an intermolecular mechanism that is completely independent of DNA
-
-
?
NAD+ + Rab5 protein
nicotinamide + (ADP-D-ribosyl)-Rab5 protein + H+
show the reaction diagram
NAD+ + Rab9 protein
nicotinamide + (ADP-D-ribosyl)-Rab9 protein + H+
show the reaction diagram
NAD+ + Ras
nicotinamide + (ADP-D-ribosyl)-Ras
show the reaction diagram
-
interaction requires residue Leu428
-
-
?
NAD+ + Ras GTPase
nicotinamide + (ADP-D-ribosyl)-Ras GTPase
show the reaction diagram
-
-
-
-
?
NAD+ + Ras2p
nicotinamide + (ADP-D-ribosyl)-Ras2p + H+
show the reaction diagram
-
Ras2p is a yeast protein, activity absolutely requires the yeast protein Bmh1p
-
-
?
NAD+ + RhoA
nicotinamide (ADP-D-ribosyl)-RhoA
show the reaction diagram
NAD+ + RhoB
nicotinamide (ADP-D-ribosyl)-RhoB
show the reaction diagram
NAD+ + RhoC
nicotinamide (ADP-D-ribosyl)-RhoC
show the reaction diagram
NAD+ + RNA polymerase
nicotinamide + (ADP-D-ribosyl)-RNA polymerase
show the reaction diagram
NAD+ + soybean trypsin inhibitor
nicotinamide + (ADP-D-ribosyl)-soybean trypsin inhibitor
show the reaction diagram
Clostridium limosum
-
the exoenzyme modifies the substrate at an arginine residue
-
-
?
NAD+ + topoisomerase I
nicotinamide + (ADP-D-ribosyl)-topoisomerase I
show the reaction diagram
NAD+ + vimentin
nicotinamide + (ADP-D-ribosyl)-vimentin
show the reaction diagram
-
-
-
-
?
NAD+ + wild-type exoenzyme C3
nicotinamide + (ADP-D-ribosyl)-wild-type exoenzyme C3
show the reaction diagram
Clostridium limosum
-
activity of the recombinant mutant Q217E exoenzyme C3, modification at Arg86
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
integrin alpha7 + NAD+
(ADP-D-ribosyl)-integrin alpha7 + nicotinamide
show the reaction diagram
-
the extracellular domain of integrin alpha7 is ADP-ribosylated by an arginine-specific ecto-ADP-ribosyltransferase after adding exogenous NAD+ to intact C2C12 muscle cells, integrin alpha7 N-terminal ADP-ribosylation inhibits the binding of integrin alpha7beta1 to laminin activation status of integrin alpha7beta1 in intact myotubes, overview
-
-
?
NAD+ + (ADP-D-ribosyl)n-acceptor
nicotinamide + (ADP-D-ribosyl)n+1-acceptor
show the reaction diagram
NAD+ + (ADP-D-ribosyl)n-acceptor
nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H+
show the reaction diagram
NAD+ + (ADP-D-ribosyl)n-p21ras
nicotinamide + (ADP-D-ribosyl)n+1-p21ras
show the reaction diagram
-
ADP-ribosylation of p21ras does not alter interactions with guanidine nucleotides. Possible function of the enzyme in pathogenesis
-
-
?
NAD+ + (ADP-D-ribosyl)n-rab4
nicotinamide + (ADP-D-ribosyl)n+1-rab4
show the reaction diagram
-
ADP-ribosylation affects Rab4 function in membrane recycling
-
-
?
NAD+ + (ADP-D-ribosyl)n-Rab5
nicotinamide + (ADP-D-ribosyl)n+1-Rab5
show the reaction diagram
-
ADP-ribosylation of Rab5 by ExoS affects endocytosis. Interaction of Rab5 with endosome antigen 1 is markedly diminished after Rab5 ADP-ribosylation by ExoS
-
-
?
NAD+ + (ADP-D-ribosyl)n-RalA
nicotinamide + (ADP-D-ribosyl)n+1-RalA
show the reaction diagram
-
ADP-ribosylation of RalA by ExoS interferes with RalA activation and binding to its downstream effector in J774A.1 macrophages and suggests the potential of ExoS ADPRT activity to interfere with filiopodium formation through the inactivation of RalA and downstream effects mediated through the exocyst complex
-
-
?
NAD+ + (ADP-D-ribosyl)n-Ras
nicotinamide + (ADP-D-ribosyl)n+1-Ras
show the reaction diagram
-
ADP-ribosylation of Ras at Arg41 disrupts Ras-Cdc25 interactions, which inhibits the rate-limiting step in Ras signal transduction, the activation of Ras by its guanine nucleotide exchange factor
-
-
?
NAD+ + cyclophilin A
nicotinamide + (ADP-D-ribosyl)-cyclophilin A
show the reaction diagram
NAD+ + histone H2A
nicotinamide + (ADP-D-ribosyl)-histone H2A
show the reaction diagram
-
the enzyme catalyses both ADP-ribosylation and deacetylation of histones, particulary H2A and H2B. Histone modification by TbSIR2RP1 is involved in DNA repair
-
-
?
NAD+ + histone H2B
nicotinamide + (ADP-D-ribosyl)-histone H2B
show the reaction diagram
-
the enzyme catalyses both ADP-ribosylation and deacetylation of histones, particulary H2A and H2B. Histone modification by TbSIR2RP1 is involved in DNA repair
-
-
?
NAD+ + moesin
nicotinamide + (ADP-D-ribosyl)-moesin
show the reaction diagram
-
ADP-ribosylated-moesin is a poor target for phosphorylation by protein kinase C and Rho kinase, which shows that ADP-ribosylation directly inhibits ERM phosphorylation
-
-
?
NAD+ + p53
nicotinamide + (ADP-D-ribosyl)-p53
show the reaction diagram
-
-
-
-
?
NAD+ + Rab5 protein
nicotinamide + (ADP-D-ribosyl)-Rab5 protein + H+
show the reaction diagram
NAD+ + Rab9 protein
nicotinamide + (ADP-D-ribosyl)-Rab9 protein + H+
show the reaction diagram
NAD+ + Ras
nicotinamide + (ADP-D-ribosyl)-Ras
show the reaction diagram
-
interaction requires residue Leu428
-
-
?
NAD+ + Ras GTPase
nicotinamide + (ADP-D-ribosyl)-Ras GTPase
show the reaction diagram
-
-
-
-
?
NAD+ + RNA polymerase
nicotinamide + (ADP-D-ribosyl)-RNA polymerase
show the reaction diagram
P12726
three ADP-ribosyltransferases, Alt, ModA, and ModB participate in the regulation of the T4 replication cycle by ADP-ribosylating a defined set of host proteins. ADP-ribosylation of RNA polymerase and of other host proteins allows initial phage-directed mRNA synthesis reactions to escape from host control
-
-
?
NAD+ + topoisomerase I
nicotinamide + (ADP-D-ribosyl)-topoisomerase I
show the reaction diagram
-
-
-
-
?
NAD+ + vimentin
nicotinamide + (ADP-D-ribosyl)-vimentin
show the reaction diagram
-
-
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
Bmh1p, a yeast homologue of the human FAS, acts as an ExoS cofactor, overview
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ba2+
-
enhances activity
Fe2+
-
enzyme binds iron through a Fe-S center, which is crucial for the catalytic activity
Sr2+
-
enhances activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0469-0796
-
restores yeast growth after treatment with ExoS
1(2H)-phthalazinone
-
IC50: 0.012 mM
1,2-benzopyrone
-
IC50: 2.8 mM
1,3-benzodiazine
-
IC50: 2.0 mM
1,3-dihydroxynaphthalene
-
IC50: 1.3 mM
1,4-benzoquinone
-
IC50: 0.4 mM
1,4-naphthalenedione
-
IC50: 0.25 mM
1,5-dihydroxyisoquinoline
-
IC50: 0.00039 mM
1,8-naphthalimide
-
IC50: 0.0014 mM
1-hydroxy-2-methyl-4-aminonaphthalene
-
IC50: 1.3 mM
1-hydroxyisoquinoline
-
IC50: 0.007 mM
1-Indanone
-
IC50: 0.81 mM
1-methylnicotinamide chloride
2,3-benzodiazine
-
IC50: 0.15 mM
2,3-dichloro-1,4-naphthoquinone
-
IC50: 0.26 mM
2,3-dihydro-1,4-phthalazinedione
-
IC50: 0.03 mM
2,3-dihydro-5-hydroxy-1,4-phthalazinedione
-
0.001 mM, 95% inhibition of the 116000 Da enzyme
-
2,4(1H,3H)-quinazolinedione
-
IC50: 0.0081 mM
2,6-difluorobenzamide
-
IC50: 0.18 mM
2-(4-[4-[(2,4-dimethoxyphenyl)amino]quinazolin-2-yl]piperazin-1-yl)ethanol
-
2-acetamidobenzamide
-
IC50: 1.0 mM
2-amino-3-chloro-1,4-naphthoquinone
-
IC50: 0.82 mM
2-Aminobenzamide
2-bromobenzamide
-
IC50: 2.9 mM
2-chlorobenzamide
-
IC50: 1.0 mM
2-fluorobenzamide
-
IC50: 0.12 mM
2-Hydroxy-1,4-naphthoquinone
-
IC50: 0.33 mM
2-hydroxybenzamide
-
IC50: 0.82 mM
2-mercapto-4(3H)-quinazolinone
-
IC50: 0.044 mM
2-Methoxybenzamide
-
IC50: 0.2 mM
2-methyl-1,4-benzopyrone
-
IC50: 0.045 mM
2-methyl-1,4-naphthoquinone
-
IC50: 0.42 mM
2-methyl-3-phytyl-1,4-naphthoquinone
-
IC50: 0.52 mM
2-methyl-4(3H)-quinazolinone
-
IC50: 0.056 mM
2-methylbenzamide
-
IC50: 1.5 mM
2-methylchromone
-
IC50: 0.045 mM
2-nitro-6(5H)-phenanthridione
-
IC50: 0.00035 mM
2-phenylchromone
-
IC50: 0.022 mM
2-trichloromethyl-4(3H)-quinazolinone
-
IC50: 2.2 mM
2H-benz[c]isoquinolin-1-one
-
IC50: 0.0003 mM
2H-benz[de]isoquinoline-1,3-dione
-
IC50: 0.0014 mM
3,4-dihydro-1(2H)-naphthalenone
-
IC50: 0.31 mM
3,5-dibromosalicylamide
-
IC50: 0.56 mM
3,5-dimethoxybenzamide
-
IC50: 1.2 mM
3,5-dinitrobenzamide
-
IC50: 2.5 mM
3-(N,N-dimethylamino)benzamide
-
IC50: 0.12 mM
3-Acetamidobenzamide
-
IC50: 0.012 mM
3-acetamidosalicylamide
-
IC50: 2.0 mM
3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole
-
i.e. Trp-P-1, 94% inhibition at 5 mM, IC50: 0.22 mM
3-amino-1-methyl-5H-pyrido[4,3-b]indole
-
i.e. Trp-P-2, 34% activation at 1 mM, 7% inhibition at 5 mM, IC50: 2.2 mM
3-aminobenzamide
3-aminobenzoic acid
-
1 mM, 12% inhibition
3-Aminophthalhydrazide
3-bromobenzamide
-
IC50: 0.055 mM
3-Chlorobenzamide
-
IC50: 0.22 mM
3-Fluorobenzamide
-
IC50: 0.2 mM
3-Guanidinobenzamide
-
0.1 mM, 71% inhibition of the 116000 Da enzyme
3-Hydroxybenzamide
3-isobutyl-1-methylxanthine
-
IC50: 3.1 mM
3-Methoxybenzamide
3-Methylbenzamide
-
IC50: 0.19 mM
3-nitrobenzamide
-
IC50: 0.16 mM
3-nitrophthalhydrazide
-
IC50: 0.072 mM
3-nitrosalicylamide
-
IC50: 1.6 mM
4,8-dihydroxy-2-quinolinecarboxylic acid
-
IC50: 0.19 mM
4-amino-1,8-naphthalimide
-
IC50: 0.00018 mM
4-Aminobenzamide
4-aminophthalhydrazide
-
IC50: 0.29 mM
4-bromobenzamide
-
IC50: 2.2 mM
4-chlorobenzamide
-
IC50: 0.3 mM
4-chromanone
-
IC50: 0.72 mM
4-fluorobenzamide
-
IC50: 0.2 mM
4-hydroxy-2-methylquinoline
-
IC50: 0.074 mM
4-hydroxy-2-quinolinecarboxylic acid
-
IC50: 0.67 mM
4-hydroxybenzamide
-
IC50: 0.28 mM
4-Hydroxycoumarin
-
IC50: 0.57 mM
4-hydroxypyridine
-
IC50: 2.3 mM
4-hydroxyquinazoline
-
IC50: 0.0095 mM
4-hydroxyquinoline
-
IC50: 0.08 mM
4-methoxybenzamide
-
IC50: 1.1 mM
4-methylbenzamide
-
IC50: 1.8 mM
4-nitrophthalhydrazide
-
IC50: 0.51 mM
4-[[[6-cyano-1-[(1-methyl-1H-imidazol-5-yl)methyl]-1,2,3,4,6,7-hexahydroquinolin-3-yl](pyridin-2-ylsulfonyl)amino]methyl]-N,N-dimethylpiperidine-1-carboxamide
-
activates in presence of Mg2+, inhibits in absence of Mg2+
4296-1011
-
restores yeast growth after treatment with ExoS
5-acetamidosalicylamide
-
IC50: 0.045 mM
5-aminosalicylamide
-
IC50: 0.1 mM
5-bromodeoxyuridine
-
IC50: 0.015 mM
5-Bromouracil
-
IC50: 0.16 mM
5-Bromouridine
-
IC50: 0.21 mM
5-chlorosalicylamide
-
IC50: 0.19 mM
5-Chlorouracil
-
IC50: 0.27 mM
5-hydroxy-1,4-naphthoquinone
-
IC50: 0.25 mM
5-Hydroxy-2-methyl-1,4-naphthoquinone
-
IC50: 0.7 mM
5-Iodouracil
-
IC50: 0.071 mM
5-iodouridine
-
IC50: 0.043 mM
5-Methylnicotinamide
5-methyluracil
-
IC50: 0.29 mM
5-Nitrouracil
-
IC50: 0.43 mM
6(5H)-phenanthridinone
-
IC50: 0.0003 mM
6-aminocoumarin
-
IC50: 0.85 mM
6-aminonicotinamide
-
IC50: 1.1 mM
8-acetamidocarsalam
-
IC50: 1.4 mM
8-Methylnicotinamide
-
IC50: 7.8 mM
acetophenone
-
IC50: 2.3 mM
ADP-D-ribose
-
5 mM, remaining activity: 7.3%
AG14361
-
-
all-trans-retinal
-
IC50: 0.45 mM
Alpha-NAD+
-
0.5 mM, 40% inhibition of the 116000 Da enzyme, 44% inhibition of the 90000 Da enzyme
alpha-picolinamide
-
IC50: 0.25 mM
AMP
-
1 mM, remaining activity: 3.6%
arachidonic acid
-
-
Benzamide
benzoyleneurea
-
IC50: 0.0081 mM
Caffeine
carbonylsalicylamide
-
IC50: 0.46 mM
carsalam
-
5 mM, 88% inhibition in presence of Mg2+, 68% inhibition in absence of Mg2+
Chlorthenoxazin
-
IC50: 0.0085 mM
chromone-2-carboxylic acid
-
IC50: 0.56 mM
Cu2+
-
-
cyclohexanecarboxamide
-
IC50: 0.62 mM
diosmin
-
restores yeast growth after treatment with ExoS
E216-5303
-
restores yeast growth after treatment with ExoS
EDTA
-
5 mM, 41% inhibition in presence of Mg2+, 2% inhibition in absence of Mg2+
everninic acid
-
restores yeast growth after treatment with ExoS
exosin
-
a small molecule inhibitor, that modulates ExoS ADP-ribosyltransferase activity in vitro, suggesting the inhibition is direct. Exosin and two of its analogues display a significant protective effect against Pseudomonas infection in vivo, competitive against NAD+
flavokawain B
-
restores yeast growth after treatment with ExoS
flavone
-
IC50: 0.022 mM
gamma-linolenic acid
-
IC50: 0.12 mM
GTP(gammaS)
-
in presence of Mg2+
Hg2+
-
-
hypoxanthine
-
IC50: 1.7 mM
Isonicotinamide
-
IC50: 0.99 mM
Isonicotinate hydrazide
-
IC50: 4.8 MM
Isoquinoline
-
5 mM, 47% inhibition in presence of Mg2+, 34% inhibition in absence of Mg2+
linoleic acid
-
IC50: 0.048 mM
linolenic acid
-
IC50: 0.11 mM
m-acetamidoacetophenone
-
IC50: 0.93 mM
m-aminoacetophenone
-
IC50: 1.9 mM
m-hydroxyacetophenone
-
IC50: 0.6 mM
m-phthalamide
-
IC50: 0.05 mM
menadione sodium bisulfite
-
IC50: 0.72 mM
N-(2-chloroethyl)1,8-naphthalamide
-
IC50: above 1.8 mM
N-(6-oxo-5,6-dihydrophenanthrolin-2-yl)-(N,N-dimethylamino)acetamide
-
0.1 mM, remaining activity: 36.2%
N-(acridin-9-yl)-4-nitrobenzamide
-
N-hydroxynaphthalimide sodium salt
-
IC50: 0.45 mM
N-[2-oxo-4-(phenylamino)-3,8a-dihydro-2H-chromen-3-yl]acetamide
-
nicotinamide
norharman
-
IC50: 4.7 mM
novobiocin
-
IC50: 2.2 mM, 5 mM, 90% inhibition in presence of Mg2+, 59% inhibition in absence of Mg2+
oleic acid
-
IC50: 0.082 mM
palmitoleic acid
-
IC50: 0.095 mM
PCMB
-
complete
phenanthridinone
-
5 mM, remaining activity: 35.1%
phthalamide
-
IC50: 1.0 mM
Phthalazine
-
5 mM, 91% inhibition in presence of Mg2+, 79% inhibition in absence of Mg2+
PJ34
-
potent PARP inhibitor
Pyrazinamide
-
IC50: 0.13 mM
Quinazoline
-
5 mM, 63% inhibition in presence of Mg2+, 50% inhibition in absence of Mg2+
reserpine
-
IC50: 0.79 mM
Theobromine
theophylline
thiobenzamide
-
IC50: 0.62 mM
Thionicotinamide
-
IC50: 1.8 mM
thymidine
trans-decahydro-1-naphthalenone
-
IC50: 4.3 mM
vitamin K1
-
IC50: 0.0019 mM
vitamin K3
-
IC50: 0.42 mM
xanthurenic acid
-
5 mM, 88% inhibition in presence of Mg2+, 65% inhibition in absence of Mg2+
Zinc acetate
-
0.1 mM, remaining activity: 0.5%
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3-amino-1-methyl-5H-pyrido[4,3-b]indole
-
i.e. Trp-P-2, 34% activation at 1 mM, 7% inhibition at 5 mM, IC50: 2.2 mM
4-[[[6-cyano-1-[(1-methyl-1H-imidazol-5-yl)methyl]-1,2,3,4,6,7-hexahydroquinolin-3-yl](pyridin-2-ylsulfonyl)amino]methyl]-N,N-dimethylpiperidine-1-carboxamide
-
activates in presence of Mg2+, inhibits in absence of Mg2+
ATP
-
5-10 mM, 20-30% stimulation
Bmh1p
-
a yeast homologue of the human FAS, acts as an activating ExoS cofactor, overview
-
FAS
-
exoenzyme S absolutely requires a soluble eukaryotic protein, named FAS (Factor Activating exoenzyme E), in order to ADP-ribosylate all substrates. In the presence of FAS, exoenzyme S ADP-ribosylates several proteins in lysates of Pseudomonas aeruginosa. Purification and characterization of FAS
-
GDP
-
increases activity in absence of Mg2+
GTP
-
increases activity in absence of Mg2+
GTP(gammaS)
-
increases activity in absence of Mg2+
harmaline hydrochloride
-
activates more strongly in absence of Mg2+ than in presence of Mg2+
Mg2+
-
5 mM, 6.3fold activation
oligodeoxyribonucleotides
-
slightly enhance enzyme activity with the maximal increase of 50% as compared to the control
Phenanthroline
-
0.1 mM, 1.1fold activation
Phthalic acid
-
activates more strongly in absence of Mg2+ than in presence of Mg2+
protein 14-3-3
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.011 - 0.037
(ADP-D-ribosyl)n-actin
0.03 - 0.429
(ADP-D-ribosyl)n-soybean-trypsin-inhibitor
0.002 - 0.154
NAD+
additional information
additional information
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.05 - 22
(ADP-D-ribosyl)n-actin
0.0031 - 0.008
(ADP-D-ribosyl)n-soybean-trypsin-inhibitor
0.0002 - 22
NAD+
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0075
2-(4-[4-[(2,4-dimethoxyphenyl)amino]quinazolin-2-yl]piperazin-1-yl)ethanol
25C, pH not specified in the publication
0.033
exosin
-
pH 6.0, 30C
0.0041
N-(acridin-9-yl)-4-nitrobenzamide
0.001
suramin
25C, pH not specified in the publication
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.012
1(2H)-phthalazinone
Bos taurus
-
IC50: 0.012 mM
2.8
1,2-benzopyrone
Bos taurus
-
IC50: 2.8 mM
2
1,3-benzodiazine
Bos taurus
-
IC50: 2.0 mM
1.3
1,3-dihydroxynaphthalene
Bos taurus
-
IC50: 1.3 mM
0.4
1,4-benzoquinone
Bos taurus
-
IC50: 0.4 mM
0.25
1,4-naphthalenedione
Bos taurus
-
IC50: 0.25 mM
0.00039
1,5-dihydroxyisoquinoline
Bos taurus
-
IC50: 0.00039 mM
0.0014
1,8-naphthalimide
Bos taurus
-
IC50: 0.0014 mM
1.3
1-hydroxy-2-methyl-4-aminonaphthalene
Bos taurus
-
IC50: 1.3 mM
0.007
1-hydroxyisoquinoline
Bos taurus
-
IC50: 0.007 mM
0.81
1-Indanone
Bos taurus
-
IC50: 0.81 mM
1.7 - 3.8
1-methylnicotinamide chloride
0.15
2,3-benzodiazine
Bos taurus
-
IC50: 0.15 mM
0.26
2,3-dichloro-1,4-naphthoquinone
Bos taurus
-
IC50: 0.26 mM
0.03
2,3-dihydro-1,4-phthalazinedione
Bos taurus
-
IC50: 0.03 mM
0.0081
2,4(1H,3H)-quinazolinedione
Bos taurus
-
IC50: 0.0081 mM
0.18
2,6-difluorobenzamide
Bos taurus
-
IC50: 0.18 mM
1
2-acetamidobenzamide
Bos taurus
-
IC50: 1.0 mM
0.82
2-amino-3-chloro-1,4-naphthoquinone
Bos taurus
-
IC50: 0.82 mM
0.1 - 0.65
2-Aminobenzamide
2.9
2-bromobenzamide
Bos taurus
-
IC50: 2.9 mM
1
2-chlorobenzamide
Bos taurus
-
IC50: 1.0 mM
0.12
2-fluorobenzamide
Bos taurus
-
IC50: 0.12 mM
0.33
2-Hydroxy-1,4-naphthoquinone
Bos taurus
-
IC50: 0.33 mM
0.82
2-hydroxybenzamide
Bos taurus
-
IC50: 0.82 mM
0.044
2-mercapto-4(3H)-quinazolinone
Bos taurus
-
IC50: 0.044 mM
0.2
2-Methoxybenzamide
Bos taurus
-
IC50: 0.2 mM
0.045
2-methyl-1,4-benzopyrone
Bos taurus
-
IC50: 0.045 mM
0.42
2-methyl-1,4-naphthoquinone
Bos taurus
-
IC50: 0.42 mM
0.52
2-methyl-3-phytyl-1,4-naphthoquinone
Bos taurus
-
IC50: 0.52 mM
0.056
2-methyl-4(3H)-quinazolinone
Bos taurus
-
IC50: 0.056 mM
1.5
2-methylbenzamide
Bos taurus
-
IC50: 1.5 mM
0.045
2-methylchromone
Bos taurus
-
IC50: 0.045 mM
0.00035
2-nitro-6(5H)-phenanthridione
Bos taurus
-
IC50: 0.00035 mM
0.022
2-phenylchromone
Bos taurus
-
IC50: 0.022 mM
2.2
2-trichloromethyl-4(3H)-quinazolinone
Bos taurus
-
IC50: 2.2 mM
0.0003
2H-benz[c]isoquinolin-1-one
Bos taurus
-
IC50: 0.0003 mM
0.0014
2H-benz[de]isoquinoline-1,3-dione
Bos taurus
-
IC50: 0.0014 mM
0.31
3,4-dihydro-1(2H)-naphthalenone
Bos taurus
-
IC50: 0.31 mM
0.56
3,5-dibromosalicylamide
Bos taurus
-
IC50: 0.56 mM
1.2
3,5-dimethoxybenzamide
Bos taurus
-
IC50: 1.2 mM
2.5
3,5-dinitrobenzamide
Bos taurus
-
IC50: 2.5 mM
0.12
3-(N,N-dimethylamino)benzamide
Bos taurus
-
IC50: 0.12 mM
0.012
3-Acetamidobenzamide
Bos taurus
-
IC50: 0.012 mM
2
3-acetamidosalicylamide
Bos taurus
-
IC50: 2.0 mM
0.22
3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole
Bos taurus
-
i.e. Trp-P-1, 94% inhibition at 5 mM, IC50: 0.22 mM
2.2
3-amino-1-methyl-5H-pyrido[4,3-b]indole
Bos taurus
-
i.e. Trp-P-2, 34% activation at 1 mM, 7% inhibition at 5 mM, IC50: 2.2 mM
0.0054 - 0.33
3-aminobenzamide
0.023
3-Aminophthalhydrazide
Bos taurus
-
IC50: 0.023 mM
0.055
3-bromobenzamide
Bos taurus
-
IC50: 0.055 mM
0.22
3-Chlorobenzamide
Bos taurus
-
IC50: 0.22 mM
0.2
3-Fluorobenzamide
Bos taurus
-
IC50: 0.2 mM
0.0091
3-Hydroxybenzamide
Bos taurus
-
IC50: 0.0091 mM
3.1
3-isobutyl-1-methylxanthine
Bos taurus
-
IC50: 3.1 mM
0.0034 - 0.017
3-Methoxybenzamide
0.19
3-Methylbenzamide
Bos taurus
-
IC50: 0.19 mM
0.16
3-nitrobenzamide
Bos taurus
-
IC50: 0.16 mM
0.072
3-nitrophthalhydrazide
Bos taurus
-
IC50: 0.072 mM
1.6
3-nitrosalicylamide
Bos taurus
-
IC50: 1.6 mM
0.19
4,8-dihydroxy-2-quinolinecarboxylic acid
Bos taurus
-
IC50: 0.19 mM
0.00018
4-amino-1,8-naphthalimide
Bos taurus
-
IC50: 0.00018 mM
0.4 - 1.8
4-Aminobenzamide
0.29
4-aminophthalhydrazide
Bos taurus
-
IC50: 0.29 mM
2.2
4-bromobenzamide
Bos taurus
-
IC50: 2.2 mM
0.3
4-chlorobenzamide
Bos taurus
-
IC50: 0.3 mM
0.72
4-chromanone
Bos taurus
-
IC50: 0.72 mM
0.2
4-fluorobenzamide
Bos taurus
-
IC50: 0.2 mM
0.074
4-hydroxy-2-methylquinoline
Bos taurus
-
IC50: 0.074 mM
0.67
4-hydroxy-2-quinolinecarboxylic acid
Bos taurus
-
IC50: 0.67 mM
0.28
4-hydroxybenzamide
Bos taurus
-
IC50: 0.28 mM
0.57
4-Hydroxycoumarin
Bos taurus
-
IC50: 0.57 mM
2.3
4-hydroxypyridine
Bos taurus
-
IC50: 2.3 mM
0.0095
4-hydroxyquinazoline
Bos taurus
-
IC50: 0.0095 mM
0.08
4-hydroxyquinoline
Bos taurus
-
IC50: 0.08 mM
1.1
4-methoxybenzamide
Bos taurus
-
IC50: 1.1 mM
1.8
4-methylbenzamide
Bos taurus
-
IC50: 1.8 mM
0.51
4-nitrophthalhydrazide
Bos taurus
-
IC50: 0.51 mM
0.006
4296-1011
Pseudomonas aeruginosa
-
pH 6.0, 30C
0.045
5-acetamidosalicylamide
Bos taurus
-
IC50: 0.045 mM
0.1
5-aminosalicylamide
Bos taurus
-
IC50: 0.1 mM
0.015
5-bromodeoxyuridine
Bos taurus
-
IC50: 0.015 mM
0.16
5-Bromouracil
Bos taurus
-
IC50: 0.16 mM
0.21
5-Bromouridine
Bos taurus
-
IC50: 0.21 mM
0.19
5-chlorosalicylamide
Bos taurus
-
IC50: 0.19 mM
0.27
5-Chlorouracil
Bos taurus
-
IC50: 0.27 mM
0.25
5-hydroxy-1,4-naphthoquinone
Bos taurus
-
IC50: 0.25 mM
0.7
5-Hydroxy-2-methyl-1,4-naphthoquinone
Bos taurus
-
IC50: 0.7 mM
0.071
5-Iodouracil
Bos taurus
-
IC50: 0.071 mM
0.043
5-iodouridine
Bos taurus
-
IC50: 0.043 mM
0.07 - 0.35
5-Methylnicotinamide
0.29
5-methyluracil
Bos taurus
-
IC50: 0.29 mM
0.43
5-Nitrouracil
Bos taurus
-
IC50: 0.43 mM
0.0003
6(5H)-phenanthridinone
Bos taurus
-
IC50: 0.0003 mM
0.85
6-aminocoumarin
Bos taurus
-
IC50: 0.85 mM
1.1
6-aminonicotinamide
Bos taurus
-
IC50: 1.1 mM
1.4
8-acetamidocarsalam
Bos taurus
-
IC50: 1.4 mM
7.8
8-Methylnicotinamide
Bos taurus
-
IC50: 7.8 mM
2.3
acetophenone
Bos taurus
-
IC50: 2.3 mM
0.45
all-trans-retinal
Bos taurus
-
IC50: 0.45 mM
0.25
alpha-picolinamide
Bos taurus
-
IC50: 0.25 mM
0.0033 - 0.22
Benzamide
0.0081
benzoyleneurea
Bos taurus
-
IC50: 0.0081 mM
1.4
Caffeine
Bos taurus
-
IC50: 1.4 mM
0.46
carbonylsalicylamide
Bos taurus
-
IC50: 0.46 mM
0.0085
Chlorthenoxazin
Bos taurus
-
IC50: 0.0085 mM
0.56
chromone-2-carboxylic acid
Bos taurus
-
IC50: 0.56 mM
0.62
cyclohexanecarboxamide
Bos taurus
-
IC50: 0.62 mM
0.003
diosmin
Pseudomonas aeruginosa
-
pH 6.0, 30C
0.023
E216-5303
Pseudomonas aeruginosa
-
pH 6.0, 30C
0.021
everninic acid
Pseudomonas aeruginosa
-
pH 6.0, 30C
0.022
flavone
Bos taurus
-
IC50: 0.022 mM
0.12
gamma-linolenic acid
Bos taurus
-
IC50: 0.12 mM
1.7
hypoxanthine
Bos taurus
-
IC50: 1.7 mM
0.99
Isonicotinamide
Bos taurus
-
IC50: 0.99 mM
4.8
Isonicotinate hydrazide
Bos taurus
-
IC50: 4.8 mM
0.048
linoleic acid
Bos taurus
-
IC50: 0.048 mM
0.11
linolenic acid
Bos taurus
-
IC50: 0.11 mM
0.93
m-acetamidoacetophenone
Bos taurus
-
IC50: 0.93 mM
1.9
m-aminoacetophenone
Bos taurus
-
IC50: 1.9 mM
0.6
m-hydroxyacetophenone
Bos taurus
-
IC50: 0.6 mM
0.05
m-phthalamide
Bos taurus
-
IC50: 0.05 mM
0.72
menadione sodium bisulfite
Bos taurus
-
IC50: 0.72 mM
1.8
N-(2-chloroethyl)1,8-naphthalamide
Bos taurus
-
IC50: above 1.8 mM
0.0858
N-(acridin-9-yl)-4-nitrobenzamide
Aeromonas hydrophila
Q49TP5
25C, pH not specified in the publication
0.45
N-hydroxynaphthalimide sodium salt
Bos taurus
-
IC50: 0.45 mM
0.086
N-[2-oxo-4-(phenylamino)-3,8a-dihydro-2H-chromen-3-yl]acetamide
Aeromonas hydrophila
Q49TP5
25C, pH not specified in the publication
0.031 - 0.21
nicotinamide
4.7
norharman
Bos taurus
-
IC50: 4.7 mM
2.2
novobiocin
Bos taurus
-
IC50: 2.2 mM, 5 mM, 90% inhibition in presence of Mg2+, 59% inhibition in absence of Mg2+
0.082
oleic acid
Bos taurus
-
IC50: 0.082 mM
0.095
palmitoleic acid
Bos taurus
-
IC50: 0.095 mM
1
phthalamide
Bos taurus
-
IC50: 1.0 mM
0.13
Pyrazinamide
Bos taurus
-
IC50: 0.13 mM
0.79
reserpine
Bos taurus
-
IC50: 0.79 mM
0.02
suramin
Aeromonas hydrophila
Q49TP5
25C, pH not specified in the publication
0.11
Theobromine
Bos taurus
-
IC50: 0.11 mM
0.046
theophylline
Bos taurus
-
IC50: 0.046 mM
0.62
thiobenzamide
Bos taurus
-
IC50: 0.62 mM
1.8
Thionicotinamide
Bos taurus
-
IC50: 1.8 mM
0.043 - 0.18
thymidine
4.3
trans-decahydro-1-naphthalenone
Bos taurus
-
IC50: 4.3 mM
0.157
V8 protease
Aeromonas hydrophila
Q49TP5
25C, pH not specified in the publication
-
0.0019
vitamin K1
Bos taurus
-
IC50: 0.0019 mM
0.42
vitamin K3
Bos taurus
-
IC50: 0.42 mM
0.077
Zn2+
Bos taurus
-
ZnCl2, IC50: 0.077 mM
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0545
-
-
0.325
-
-
1.02
-
-
80
Clostridium limosum
-
wild-type enzyme, substrate RhoA
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.5
-
reaction with histone in presence of DNA, 0.1 M glycine-NaOH buffer
8.7
-
reaction with histone in presence of DNA, 0.1 M Tris-HCl buffer
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 9.5
-
pH 7.0: about 75% of maximal activity, pH 9.5: about 70% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
-
116000 Da enzyme
10
-
90000 Da enzyme
30 - 37
Clostridium limosum
-
assay at
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.3
-
isoelectric focusing. ExoS shows pI heterogeneity, that is independent of a mass change and thus represents molecular charge conformers
5.5
-
isoelectric focusing. ExoS shows pI heterogeneity, that is independent of a mass change and thus represents molecular charge conformers
5.7
-
isoelectric focusing. ExoS shows pI heterogeneity, that is independent of a mass change and thus represents molecular charge conformers
5.9
-
isoelectric focusing. ExoS shows pI heterogeneity, that is independent of a mass change and thus represents molecular charge conformers
7 - 7.2
-
; isoelectric focusing
9.3
-
theoretical value
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
transfectants expressing either ART2.2 with its native anchor (RT2.2-GPI) or ART2.2 with a grafted transmembrane anchor
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
TbSIR2RP1 is a nuclear protein that colocalizes with telomeric sequences and minichromosomes
Manually annotated by BRENDA team
-
10% of the activity
Manually annotated by BRENDA team
-
localizes in eukaryotic cells to the perinuclear region
-
Manually annotated by BRENDA team
-
despite the absence of a signal peptide, the protein is efficiently exported into periplasm
-
Manually annotated by BRENDA team
additional information
PDB
SCOP
CATH
ORGANISM
UNIPROT