Information on EC 2.8.2.20 - protein-tyrosine sulfotransferase

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The expected taxonomic range for this enzyme is: Eukaryota

EC NUMBER
COMMENTARY hide
2.8.2.20
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RECOMMENDED NAME
GeneOntology No.
protein-tyrosine sulfotransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3'-phosphoadenylyl sulfate + protein tyrosine = adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sulfate group transfer
SYSTEMATIC NAME
IUBMB Comments
3'-phosphoadenylyl-sulfate:protein-tyrosine O-sulfotransferase
The tyrosine residues of some specific proteins of rat pheochromocytoma cells act as acceptors.
CAS REGISTRY NUMBER
COMMENTARY hide
87588-33-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
ecotypes Columbia, C24, Landsberg erecta, and Wassilewskija
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
leech
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Manually annotated by BRENDA team
isoforms TPST1 and TPST2
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
no activity in prokaryotes
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Manually annotated by BRENDA team
no activity in yeast
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + Ac-YLFSVHWPPLNK(COOSu)A-OH
adenosine 3',5'-bisphosphate + Ac-Y(O-sulfate)LFSVHWPPLNK(COOSu)A-OH
show the reaction diagram
-
an N-succinimidyl carbamate modified peptide substrate
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-
?
3'-phosphoadenylyl sulfate + Ac-YLFSVHWPPLNKA-OH
adenosine 3',5'-bisphosphate + Ac-Y(O-sulfate)LFSVHWPPLNKA-OH
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + benzyl-Glu-Tyr
adenosine 3',5'-bisphosphate + benzyl-Glu-Tyr-O-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + C3aR tyrosine
adenosine 3',5'-bisphosphate + C3aR tyrosine O-sulfate
show the reaction diagram
-
C3a-anaphylatoxin chemotactic receptor,C3aR. Sulfation sequence motif is HNRCGY174KFGLSSSLDY184PDFY188 GDPLEQGFQDY317Y318NLGQF
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-
?
3'-phosphoadenylyl sulfate + C5aR tyrosine
adenosine 3',5'-bisphosphate + C5aR tyrosine O-sulfate
show the reaction diagram
-
C5a-anaphylatoxin chemotactic receptor. Sulfation sequence motif is TTPDY11GHY14DDKDTLD
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-
?
3'-phosphoadenylyl sulfate + CC receptor tyrosine
adenosine 3',5'-bisphosphate + CC receptor tyrosine O-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + CCR5 1-18 peptide
adenosine 3',5'-bisphosphate + sulfated CCR5 1-18peptide
show the reaction diagram
-
substrate is a synthetic human CCR5 peptide MDYQVSSPIYDINYYTSE-NH2. Sulfation pattern: Tyr3 is sulfated first, followed by Tyr14 or Tyr15, and finally Tyr10
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-
?
3'-phosphoadenylyl sulfate + CCR5 2-18 peptide
adenosine 3',5'-bisphosphate + sulfated CCR5 2-18peptide
show the reaction diagram
-
substrate is a synthetic human CCR5 peptide DYQVSSPIYDINYYTSE-NH2. Sulfation pattern: Tyr14 and Tyr15 are sulfated first, followed by Tyr10, with Tyr3 sulfated last
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-
?
3'-phosphoadenylyl sulfate + CCR8 1-17 M1A peptide
adenosine 3',5'-bisphosphate + sulfated CCR8 1-17 M1A peptide
show the reaction diagram
3'-phosphoadenylyl sulfate + CCR8 2-17 M1A peptide
adenosine 3',5'-bisphosphate + sulfated CCR8 2-17 M1A peptide
show the reaction diagram
3'-phosphoadenylyl sulfate + CX3CR1 tyrosine
adenosine 3',5'-bisphosphate + CX3CR1 tyrosine O-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + CXC-chemokine receptor 4
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylyl sulfate + CXCR4 1-38
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
CC-chemokine receptor 4 peptide, amino acids 1-38
the enzyme has a strong site preference for sulfation of Tyr-21 over Tyr-7 and Tyr-12, sulfation of Tyr-21 is required for high-affinity interaction with stromal cell-derived factor-1 (SDF-1alpha)
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?
3'-phosphoadenylyl sulfate + CXCR4 peptide tyrosine
adenosine 3',5'-bisphosphate + CXCR4 peptide tyrosine O-sulfate
show the reaction diagram
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a cytokine receptor peptide fragment
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?
3'-phosphoadenylyl sulfate + EDYDTTTEFDsYGD
adenosine 3',5'-bisphosphate + EDsYDTTTEFDsYGD
show the reaction diagram
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G protein-coupled receptor, human chemokine receptor 1, amino acids 8-20
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-
?
3'-phosphoadenylyl sulfate + EDYDTTTEFDYGD
adenosine 3',5'-bisphosphate + EDYDTTTEFDsYGD
show the reaction diagram
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G protein-coupled receptor human chemokine receptor 1, amino acids 8-20
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?
3'-phosphoadenylyl sulfate + EEPEYGE
adenosine 3',5'-bisphosphate + EEPEY(O-sulfate)GE
show the reaction diagram
-
-
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-
?
3'-phosphoadenylyl sulfate + ENYSYDLDYYS
adenosine 3',5'-bisphosphate + ENsYSsYDLDsYsYS
show the reaction diagram
-
G protein-coupled receptor human gpr1, amino acids 13-23, sulfation order: first Tyr21, second Tyr22, third Tyr15 or 17, fourth Tyr 15 or 17
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?
3'-phosphoadenylyl sulfate + FSHR tyrosine
adenosine 3',5'-bisphosphate + FSHR tyrosine O-sulfate
show the reaction diagram
-
follicle-stimulating hormone receptor, FSHR. Sulfation sequence motif is F-D/E-Y335
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?
3'-phosphoadenylyl sulfate + glutathione S-transferase-tagged P-selectin glycoprotein ligand-1
adenosine 3',5'-bisphosphate + glutathione S-transferase-tagged P-selectin glycoprotein ligand-1 tyrosine O-sulfate
show the reaction diagram
-
-
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?
3'-phosphoadenylyl sulfate + human anionic trypsinogen tyrosine
adenosine 3',5'-bisphosphate + trypsinogen tyrosine O-sulfate
show the reaction diagram
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i.e. serine protease 2, sulfation of Tyr154 facilitated by a unique sequence context which is characteristically found in primate trypsinogens
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?
3'-phosphoadenylyl sulfate + LHR/CGR tyrosine
adenosine 3',5'-bisphosphate + LHR/CGR tyrosine O-sulfate
show the reaction diagram
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luteinising hormone receptor, LHR/chorionic gonadotropin receptor, CGR. Sulfation sequence motif is Y385-D/E-Y
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?
3'-phosphoadenylyl sulfate + Mfge8 protein tyrosine
adenosine 3',5'-bisphosphate + Mfge8 protein tyrosine O-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + P-selectin glycoprotein ligand-1
adenosine 3',5'-bisphosphate + P-selectin glycoprotein ligand-1 tyrosine O-sulfate
show the reaction diagram
-
i.e. ATEYEYLDYDFL
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?
3'-phosphoadenylyl sulfate + P-selectin glycoprotein ligand-1 tyrosine
adenosine 3',5'-bisphosphate + P-selectin glycoprotein ligand-1 tyrosine O-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + peptide C4P5Y3
?
show the reaction diagram
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-
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?
3'-phosphoadenylyl sulfate + poly-Glu6Ala3Tyr1 peptide
adenosine 3',5'-bisphosphate + poly-Glu6Ala3Tyrsulfate peptide
show the reaction diagram
-
-
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-
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine O-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + PSGL-1
adenosine 3',5'-bisphosphate + PSGL-1 sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + PSGL-1 protein
adenosine 3',5'-bisphosphate + PSGL-1 protein tyrosine-O-sulfate
show the reaction diagram
-
i.e. ATEYEYLDYDFL
-
-
?
3'-phosphoadenylyl sulfate + QATEYEYLDYDFLPE
adenosine 3',5'-bisphosphate + QATEsYEsYLDsYDFLPE
show the reaction diagram
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human PSGL-1, amino acids 42-56, sulfation order: first Tyr51, second Tyr46 or 48, third Tyr46 or 48
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?
3'-phosphoadenylyl sulfate + QATEYEYLDYDFLPE-NH2
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
P-selectin glycoprotein ligand-1, amino acids 1-15
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?
3'-phosphoadenylyl sulfate + RNase 9 protein tyrosine
adenosine 3',5'-bisphosphate + RNase 9 protein tyrosine O-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + ROL-6 protein tyrosine
adenosine 3',5'-bisphosphate + ROL-6 protein tyrosine-O-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + S1P1R tyrosine
adenosine 3',5'-bisphosphate + S1P1R tyrosine O-sulfate
show the reaction diagram
-
type 1 Sphingosine 1-phosphate receptor, S1P1R. Sulfation sequence motif is D-Y19-V/G/-N-Y22-D
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?
3'-phosphoadenylyl sulfate + statherin
adenosine 3',5'-bisphosphate + statherin O-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + statherin
adenosine 3',5'-bisphosphate + statherin-O-sulfate
show the reaction diagram
-
a 43-residue polypeptide, which is tyrosine-rich and contains 7 tyrosine residues
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?
3'-phosphoadenylyl sulfate + SYGDEYPDYLD
adenosine 3',5'-bisphosphate + SsYGDEsYPDsYLD
show the reaction diagram
-
G protein-coupled receptor human dez, amino acids 13-23, sulfation order: first Tyr21, second Tyr18, third Tyr14
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?
3'-phosphoadenylyl sulfate + trypsinogen tyrosine
adenosine 3',5'-bisphosphate + trypsinogen tyrosine O-sulfate
show the reaction diagram
-
sulfation of Tyr154 with Asp as important determinant. Mutation D153N, a naturally occuring polymorphisms in African population, results in a complete loss of trypsinogen sulfation, while mutation of Gly151 and Pro155 of the trypsinogen has no effect on enzyme activity. Genotyping of different populations for the substrate polymorphism, overview
-
-
?
3'-phosphoadenylyl sulfate + TSH receptor
adenosine 3',5'-bisphosphate + TSH receptor O-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + TSHR tyrosine
adenosine 3',5'-bisphosphate + TSHR tyrosine O-sulfate
show the reaction diagram
-
thyroid-stimulating hormone receptor, TSHR. Sulfation sequence motif is Y385-D/E-Y
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-
?
3'-phosphoadenylyl sulfate + TTFFDsYDYGA
adenosine 3',5'-bisphosphate + TTFFDsYDsYGA
show the reaction diagram
-
G protein-coupled receptor, human chemokine receptor 2, amino acids 21-30
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-
?
3'-phosphoadenylyl sulfate + TTFFDYDsYGA
adenosine 3',5'-bisphosphate + TTFFDsYDsYGA
show the reaction diagram
-
G protein-coupled receptor, human chemokine receptor 2, amino acids 21-30
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-
?
3'-phosphoadenylyl sulfate + TTFFDYDYGA
adenosine 3',5'-bisphosphate + TTFFDsYDYGA
show the reaction diagram
-
G protein-coupled receptor, human chemokine receptor 2, amino acids 21-30
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-
?
3'-phosphoadenylyl sulfate + TTFFDYDYGA
adenosine 3',5'-bisphosphate + TTFFDYDsYGA
show the reaction diagram
-
G protein-coupled receptor, human chemokine receptor 2, amino acids 21-30
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-
?
3'-phosphoadenylyl sulfate + Tyr-Tyr-Tyr
adenosine 3',5'-bisphosphate + Tyr-Tyr-Tyr-O-sulfate
show the reaction diagram
-
-
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?
3'-phosphoadenylyl sulfate + vitronectin peptide
adenosine 3',5'-bisphosphate + vitronectin-O-sulfate
show the reaction diagram
-
amino acids 75-91, Y(SO3H)TVY(SO3H)DDGEEKNDATVHE
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-
?
3'-phosphoadenylyl sulfate + VTDsYsYYPDI
adenosine 3',5'-bisphosphate + VTDsYsYsYPDI
show the reaction diagram
-
Tyr16-sulfated G protein-coupled receptor CCR8, amino acids 12-20
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?
3'-phosphoadenylyl sulfate + VTDsYYsYPDI
adenosine 3',5'-bisphosphate + VTDsYsYsYPDI
show the reaction diagram
-
CC-chemokine receptor 8 nonapeptide, sY15CCR8
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-
?
3'-phosphoadenylyl sulfate + VTDsYYYPDG
adenosine 3',5'-bisphosphate + VTDsYYsYPDI
show the reaction diagram
-
CC-chemokine receptor 8 nonapeptide, sY15sY17CCR8
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-
?
3'-phosphoadenylyl sulfate + VTDsYYYPDI
adenosine 3',5'-bisphosphate + VTDsYYsYPDI
show the reaction diagram
3'-phosphoadenylyl sulfate + VTDYsYYPDI
adenosine 3',5'-bisphosphate + VTDsYsYYPDI
show the reaction diagram
-
Tyr16-sulfated G protein-coupled receptor CCR8, amino acids 12-20
-
-
?
3'-phosphoadenylyl sulfate + VTDYYsYPDI
adenosine 3',5'-bisphosphate + VTDsYYsYPDI
show the reaction diagram
-
both isoenzymes sequentially sulfate the CCR8 peptide such that Tyr15 is sulfated first followed by Tyr17 sulfation
-
-
?
3'-phosphoadenylyl sulfate + VTDYYYPDI
adenosine 3',5'-bisphosphate + VTDsYYYPDI
show the reaction diagram
3'-phosphoadenylyl sulfate + VTDYYYPDI
adenosine 3',5'-bisphosphate + VTDYYsYPDI
show the reaction diagram
-
a peptide modeled on the N-terminal extracellular domain of human CCR8, amino acids 12-20
-
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?
3'-phosphoadenylyl-sulfate + ATEFEFLDYDFL
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
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?
3'-phosphoadenylyl-sulfate + ATEFEYLDYDFL
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
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?
3'-phosphoadenylyl-sulfate + ATEYEFLDYDFL
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl-sulfate + ATEYEYLDYDFL
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl-sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
show the reaction diagram
-
TPST-A is involved in the cuticle formation in the nematode. TPST-A RNAi significantly decreases the dityrosine level in the worms, raising the possibility that the sulfation process may be a pre-requisite for the collagen tyrosine cross-linking
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?
3'-phosphoadenylylsulfate + acidic amino acid polymer EAY
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + acidic polypeptide tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + alpha-tubulin
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
peptide PKV
-
-
?
3'-phosphoadenylylsulfate + C-terminal peptide fragments of complement component C4
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + C4 alpha chain
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
more efficiently sulphated by TPST1 than TPST2
-
-
?
3'-phosphoadenylylsulfate + DYIYTQDVK
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + DYQVSSPIYDINYYTSE
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
amino acids 2-18 derived from peptide CC-chemokine receptor 5, final product has four sulfotyrosine residues, sulfation occurs in the following order: Tyr13, Tyr14, Tyr9, Tyr2
-
-
?
3'-phosphoadenylylsulfate + EANEDFEDYEFDE
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
i.e. P395A
-
-
?
3'-phosphoadenylylsulfate + EEFHTDYIYTQDVK
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + FHTDYIYTQDVK
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + heparin cofactor II
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
more efficiently sulphated by TPST1 than TPST2
-
-
?
3'-phosphoadenylylsulfate + KKE(EPEYGE)2
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + KKE(EPEYGE)3
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + KKEEPEYGE
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + KKEEPEYGE(EPEFGE)2
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + KKSAEDYEYPSMG
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + KKSAEDYQYPSMG
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + KKSAENYEYPSMG
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + KKSAENYQYPSMG
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + PKG
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + preprocholecystokinin
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + protein tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + QATEYEYLDYDFLPE
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
derived from N-terminal residues of the mature leukocyte adhesion molecule P-selectin glycoprotein ligand 1
-
-
?
3'-phosphoadenylylsulfate + QATEYEYLDYDFLPEC
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + recombinant hirudin variants
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + synthetic peptides
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + tert-butoxycarbonylcholecystokinin octapeptide
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
BOC-CCK
-
-
?
3'-phosphoadenylylsulfate + WEEFHTDYIYTQDVK
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + C3aR tyrosine
adenosine 3',5'-bisphosphate + C3aR tyrosine O-sulfate
show the reaction diagram
-
C3a-anaphylatoxin chemotactic receptor,C3aR. Sulfation sequence motif is HNRCGY174KFGLSSSLDY184PDFY188 GDPLEQGFQDY317Y318NLGQF
-
-
?
3'-phosphoadenylyl sulfate + C5aR tyrosine
adenosine 3',5'-bisphosphate + C5aR tyrosine O-sulfate
show the reaction diagram
-
C5a-anaphylatoxin chemotactic receptor. Sulfation sequence motif is TTPDY11GHY14DDKDTLD
-
-
?
3'-phosphoadenylyl sulfate + CC receptor tyrosine
adenosine 3',5'-bisphosphate + CC receptor tyrosine O-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + CX3CR1 tyrosine
adenosine 3',5'-bisphosphate + CX3CR1 tyrosine O-sulfate
show the reaction diagram
-
a cytokine receptor for CXCL10/IP-10 and CXCL11/I-TAC, contains two potential tyrosine sulfation sites within the sequence ENFSSSY27DY29GENESDS
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-
?
3'-phosphoadenylyl sulfate + CXC-chemokine receptor 4
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
O60704
CXCR4 signaling is indispensable for embryonic development
-
-
?
3'-phosphoadenylyl sulfate + FSHR tyrosine
adenosine 3',5'-bisphosphate + FSHR tyrosine O-sulfate
show the reaction diagram
-
follicle-stimulating hormone receptor, FSHR. Sulfation sequence motif is F-D/E-Y335
-
-
?
3'-phosphoadenylyl sulfate + glutathione S-transferase-tagged P-selectin glycoprotein ligand-1
adenosine 3',5'-bisphosphate + glutathione S-transferase-tagged P-selectin glycoprotein ligand-1 tyrosine O-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + LHR/CGR tyrosine
adenosine 3',5'-bisphosphate + LHR/CGR tyrosine O-sulfate
show the reaction diagram
-
luteinising hormone receptor, LHR/chorionic gonadotropin receptor, CGR. Sulfation sequence motif is Y385-D/E-Y
-
-
?
3'-phosphoadenylyl sulfate + Mfge8 protein tyrosine
adenosine 3',5'-bisphosphate + Mfge8 protein tyrosine O-sulfate
show the reaction diagram
-
the long isoform of Mfge8, substrate of isozyme TPST-2, not isozyme TPST-1, mutational analysis, overview
-
-
?
3'-phosphoadenylyl sulfate + P-selectin glycoprotein ligand-1
adenosine 3',5'-bisphosphate + P-selectin glycoprotein ligand-1 tyrosine O-sulfate
show the reaction diagram
-
i.e. ATEYEYLDYDFL
-
-
?
3'-phosphoadenylyl sulfate + P-selectin glycoprotein ligand-1 tyrosine
adenosine 3',5'-bisphosphate + P-selectin glycoprotein ligand-1 tyrosine O-sulfate
show the reaction diagram
-
sulfoTyr plays a functional role is P-selectin glycoprotein ligand-1, which is involved in leukocyte-mediated inflammation
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine O-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + PSGL-1 protein
adenosine 3',5'-bisphosphate + PSGL-1 protein tyrosine-O-sulfate
show the reaction diagram
-
i.e. ATEYEYLDYDFL
-
-
?
3'-phosphoadenylyl sulfate + RNase 9 protein tyrosine
adenosine 3',5'-bisphosphate + RNase 9 protein tyrosine O-sulfate
show the reaction diagram
-
substrate of isozyme TPST-2, not isozyme TPST-1, mutational analysis, overview
-
-
?
3'-phosphoadenylyl sulfate + ROL-6 protein tyrosine
adenosine 3',5'-bisphosphate + ROL-6 protein tyrosine-O-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + S1P1R tyrosine
adenosine 3',5'-bisphosphate + S1P1R tyrosine O-sulfate
show the reaction diagram
-
type 1 Sphingosine 1-phosphate receptor, S1P1R. Sulfation sequence motif is D-Y19-V/G/-N-Y22-D
-
-
?
3'-phosphoadenylyl sulfate + statherin
adenosine 3',5'-bisphosphate + statherin O-sulfate
show the reaction diagram
-
TPST is responsible for the sulfation of a variety of secretory and membrane proteins, e.g. of the salivary secretory protein, statherin
-
-
?
3'-phosphoadenylyl sulfate + trypsinogen tyrosine
adenosine 3',5'-bisphosphate + trypsinogen tyrosine O-sulfate
show the reaction diagram
-
sulfation of Tyr154 with Asp as important determinant. Mutation D153N, a naturally occuring polymorphisms in African population, results in a complete loss of trypsinogen sulfation, while mutation of Gly151 and Pro155 of the trypsinogen has no effect on enzyme activity. Genotyping of different populations for the substrate polymorphism, overview
-
-
?
3'-phosphoadenylyl sulfate + TSH receptor
adenosine 3',5'-bisphosphate + TSH receptor O-sulfate
show the reaction diagram
-
tyrosine sulfonation of TSHR by TPST2 is crucial for thyrotropin signaling, TPST1 and TPST2 mutant H266Q are incapable of mediating the signal transduction, overview
-
-
?
3'-phosphoadenylyl sulfate + TSHR tyrosine
adenosine 3',5'-bisphosphate + TSHR tyrosine O-sulfate
show the reaction diagram
-
thyroid-stimulating hormone receptor, TSHR. Sulfation sequence motif is Y385-D/E-Y
-
-
?
3'-phosphoadenylyl sulfate + VTDsYYYPDI
adenosine 3',5'-bisphosphate + VTDsYYsYPDI
show the reaction diagram
-
-
-
-
ir
3'-phosphoadenylyl sulfate + VTDYYYPDI
adenosine 3',5'-bisphosphate + VTDsYYYPDI
show the reaction diagram
-
-
-
-
ir
3'-phosphoadenylyl-sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
show the reaction diagram
-
TPST-A is involved in the cuticle formation in the nematode. TPST-A RNAi significantly decreases the dityrosine level in the worms, raising the possibility that the sulfation process may be a pre-requisite for the collagen tyrosine cross-linking
-
-
?
3'-phosphoadenylylsulfate + protein tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
activation by 10 mM divalent cations in decreasing order of efficiency: Mn2+, Ca2+, Cu2+, Mg2+, Zn2+
Cu2+
-
activation by 10 mM divalent cations in decreasing order of efficiency: Mn2+, Ca2+, Cu2+, Mg2+, Zn2+
Divalent cations
-
requirement
MnCl2
-
required, maximal activity at 20 mM
NaCl
-
activation, sulfation of tert-butoxycarbonylcholecystokinin
Zn2+
-
activation by 10 mM divalent cations in decreasing order of efficiency: Mn2+, Ca2+, Cu2+, Mg2+, Zn2+
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,6-Dichloro-4-nitrophenol
3'-phosphoadenylyl sulfate
-
-
5'-AMP
-
inhibits about 50% at 2 mM
Acidic amino acid polymer
-
excess concentrations
-
adenosine 3',5'-bisphosphate
Cu2+
10 mM, complete inhibition
D-erythro-sphinganine
-
less effective than sphingosine
dithiothreitol
-
-
Fe2+
10 mM, complete inhibition
Hg2+
10 mM, complete inhibition
L-threo-sphinganine
-
-
Lubrol Px
-
3fold stimulation at 0.1% w/v, weak inhibition above 0.1% w/v
Mn2+
-
above 30 mM, activation below
N-ethylmaleimide
phosphatidylcholine
-
weak
Poly-EAY
psychosine
-
almost complete inhibition
sphingomyelin
-
weak
sphingosine
-
50% inhibition at 0.15 mM, kinetics, enhanced by phosphatidylcholine or sphingomyelin. Phosphatidylinositol, phosphatidylserine or oleic acid reverses
Stearylamine
-
-
Triton X-100
-
inhibits about 50% at 0.25%
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5'-AMP
6-keto-prostaglandin F1alpha
-
23% activation at 0.1 mM
CHAPS
-
activation, 0.5% w/v
dithiothreitol
-
1 mM
Lubrol Px
lysophosphatidylcholine
-
slight activation, 0.2 mM
Mg2+
-
strongly stimulates isozyme TPST-2, but not TPST-1
Mn2+
-
strong activation of both isozymes
Nonidet P-40
oleic acid
-
activation, 0.2 mM
phosphatidylinositol
-
slight activation, 0.2 mM
phosphatidylserine
-
slight activation, 0.2 mM
prostaglandin E2
-
1.5fold stimulation at 0.1 mM, half maximal stimulation at 0.05 mM
prostaglandin F2alpha
-
22% activation at 0.1 mM
sodium dodecylsulfate
-
2fold stimulation at 0.1%
Sofalcone
-
55% stimulation
Triton X-100
Tween 20
Tween 80
-
activation, 0.5% w/v
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0021 - 0.59
3'-phosphoadenylyl sulfate
0.00025 - 0.02
3'-phosphoadenylylsulfate
0.0315
ATEFEFLDYDFL
pH 5.8, 28C
0.0085
ATEFEYLDYDFL
pH 5.8, 28C
0.0052
ATEYEFLDYDFL
pH 5.8, 28C
0.0359
ATEYEYLDYDFL
pH 5.8, 28C
1.223 - 1.297
benzyl-Glu-Tyr
0.017
CCK-(107-115)
-
pH 6.5, 30C
0.00004 - 0.003
EAY
0.071 - 0.457
EEFHTDYIYTQDVK
0.0017
KKE(EPEYGE)2
-
pH 6.5, 30C
0.000044
KKE(EPEYGE)3
-
pH 6.5, 30C
-
0.043
KKEEPEYGE
-
pH 6.5, 30C
0.00034
KKEEPEYGE(EPEFGE)2
-
pH 6.5, 30C
0.021
KKSAEDYEYPSMG
-
pH 6.5, 30C
0.37
KKSAEDYQYPSMG
-
pH 6.5, 30C
0.55
KKSAENYEYPSMG
-
pH 6.5, 30C
0.59
KKSAENYQYPSMG
-
pH 6.5, 30C
0.011 - 0.053
P-selectin glycoprotein ligand-1
0.11
peptide CCK-(107-115)
-
pH 6.5, 30C
0.15
peptide CCK-2
-
pH 6.5, 30C
0.12
peptide CCK-3
-
pH 6.5, 30C
0.035
peptide CCKI
-
pH 6.5, 30C
-
0.0015 - 0.0016
peptide EAY
0.24 - 0.41
peptide Hir-(57-65)
0.14
peptide tub-1
-
pH 6.5, 30C
0.0097 - 0.0269
PSGL-1
5.6
recombinant hirudin HV2
-
pH 6.5, 30C
-
0.04
statherin
0.0175 - 0.022
Tyr-Tyr-Tyr
2 - 21
VTDsYYsYPDI
0.0031
VTDsYYYPDI
-
in 20 mM MOPS, at pH 7.5, 100 mM NaCl, at 30C
-
0.0193 - 120
VTDYYYPDI
additional information
additional information
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.058 - 0.11
3'-phosphoadenylyl sulfate
0.045 - 0.1
P-selectin glycoprotein ligand-1
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
6.7 - 18.2
3'-phosphoadenylyl sulfate
2 - 4.2
P-selectin glycoprotein ligand-1
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.031 - 0.061
3'-phosphoadenylyl sulfate
0.00016 - 0.00074
adenosine 3',5'-bisphosphate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0000031
recombinant TPST-1 in HEK-293T cell extract
0.0000032
recombinant TPST-2 in HEK-293T cell extract
0.0057
-
-
additional information
-
development of a specific detection method using an anti-sulfotyrosine monoclonal antibody, termed PSG2, that binds with high affinity and exquisite specificity to sulfotyrosine residues in peptides and proteins independently of sequence context, overview
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 6.5
-
Golgi-enzyme, sulfation of tert-butoxycarbonylcholecystokinin
6.2 - 6.8
-
-
6.3
-
-
6.4 - 6.6
-
solubilized enzyme
7.2
-
assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 6.5
pH 5.5: about 95% of maximal activity, pH 6.5: about 55% of maximal activity
5.7 - 6.8
-
about half-maximal activity at pH 5.7 and 6.8
6.2 - 7.2
-
sulfation of acidic amino acid polymer EAY, liver, about half-maximal activity at pH 6.2 and 7.2
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22
-
assay at room temperature
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
TPST-2 shows higher expression as TPST-1
Manually annotated by BRENDA team
-
primary culture of umbilical vein endothelial cells, 2 isoforms: TPST1 and TPST2, shear stress causes a shift in protein expression from TPST1 to TPST2
Manually annotated by BRENDA team
-
cauda
Manually annotated by BRENDA team
-
primary
Manually annotated by BRENDA team
-
mainly expressed in hypodermis, especially in the seam cells
Manually annotated by BRENDA team
-
breast cancer cell line, isozyme TPST-2
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
TPST-1 displays type II transmembrane topology with a short N-terminal cytoplasmic domain, a single 17-residue transmembrane domain, and a luminal catalytic domain; TPST-2 displays type II transmembrane topology with a short N-terminal cytoplasmic domain, a single 17-residue transmembrane domain, and a luminal catalytic domain
Manually annotated by BRENDA team
-
in the trans-Golgi network the TPST is anchored to the membrane with its catalytic domain luminally oriented; in the trans-Golgi network the TPST is anchored to the membrane with its catalytic domain luminally oriented
Manually annotated by BRENDA team
additional information
-
subcellular distribution
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
42130
calculated from nucleotide sequence
42190
-
calculated from nucleotide sequence
50000 - 54000
54000
-
SDS-PAGE
100000
-
enzyme-detergent micelle, glycerol gradient centrifugation
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
determination of crystal and three dimensional structures of the isozymes
-
in complex with substrate peptide C4P5Y3
-
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
half-life of 30 h in vivo
-
stable in cell lysates prepared by freeze-thawing in 100 mM HEPES-NaOH buffer, pH 7.4, 20 mM MgCl2, 10 mM mercaptoethanol or in 5 mM EDTA or 0.32 M sucrose or by hypoosmotic lysis using 5 mM EDTA, no activity in cell lysates prepared in the presence of Triton X-100 or by sonication
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-40C, in 10 mM Tris-HCl, pH 7.4, 25% glycerol, 5 mM mercaptoethanol, 1% Triton X-100, 3 days
-
-80C, in 10 mM HEPES-buffer, pH 7, 50 mM NaCl, 1% Triton X-100, 1 mM DTT, 25% glycerol, several months
-
-80C, in 25% glycerol, 30% loss of activity within 1 month
-
4C, detergent-solubilized enzyme preparation, t1/2: 48 h
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2 recombinant isoforms: TPST1 and TPST2
-
anti-protein C immunoaffinity chromatography; anti-protein C immunoaffinity chromatography
-
develoment of an immunoaffinity purification method using anti-sulfotyrosine monoclonal antibody PSG2, recombinantly expressed in CHO cells
-
homogeneity
-
HPC4 affinity column chromatography, and gel filtration
-
liver; partial
-
Ni-NTA agarose bead chromatography, and gel filtration
-
partial
recombinant enzyme
-
treatment of membrane-bound enzyme with carbonate, followed by solubilization and affinity chromatography on a substrate peptide
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2 isoforms: TPST1 and TPST2
-
expressed in CHO cells
-
expressed in CHO-K1 cells
-
expressed in Escherichia coli BL21(DE3) cells
-
expressed in HEK-293 cells
-
expression in COS-7 cells
expression in HEK-293T cells; expression in HEK-293T cells
-
expression of isozyme TPST-1 in HEK-293T cells, quantitative real-time PCR expression analysis; expression of isozyme TPST-2 in HEK-293T cells, quantitative real-time PCR expression analysis
expression of TPSt-1 in HeLa cells and COS-7 cells as GFP-tagged protein in the Golgi compartment
-
gene Tpst2, wild-type and mutant enzymes genotyping and genetic mapping, expression of genes Tpst1 and Tpst2 in grt mouse cells, rescue of the phenotype, overview
-
isozyme TPST-2, expression analysis
-
isozymes TPST-1 and TPST-2, DNA and amino acid sequence determinationand analysis, sequence comparison
-
isozymes TPST-1 and TPSt-2, geno- and phenotyping of wild-types and mutants
-
isozymes TPST-1 and TPST-2, genotyping
-
isozymes TPST-1 and TPST-2. TPST-1 DNA and amino acid sequence determinationand analysis, sequence comparison
-
substance protein A-fused tyrosylprotein sulfotransferase is expressed in Escherichia coli BL21(DE3) cells
-
TPST DNA and amino acid sequence determinationand analysis, sequence comparison
-
TPST-1, sequence comparison
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
N262A
-
site-directed mutagenesis, the N-glycosylation site mutant is less N-glycosylated and has a lower molecular weight compared to the wild-type enzyme
N60A
-
site-directed mutagenesis, the N-glycosylation site mutant is less N-glycosylated and has a lower molecular weight compared to the wild-type enzyme
H266Q
-
missense mutation of Tpst2, devoid of activity, in the highly conserved region of the gene leads to the autosomal recessive, fetal-onset, severe thyroid hypoplasia phenotype related to TSH hyporesponsiveness in growth-retarded mice, the mutant enzyme shows unaltered subcellular localization, overview
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
molecular biology
-
conjugation of proteins with N-carbamoyl-succinate-modified peptides is an appropriate tool in research, for instance, in the development of vaccines and drugs or for studying biological mechanisms