Information on EC 2.8.2.20 - protein-tyrosine sulfotransferase

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The expected taxonomic range for this enzyme is: Eukaryota

EC NUMBER
COMMENTARY
2.8.2.20
-
RECOMMENDED NAME
GeneOntology No.
protein-tyrosine sulfotransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
3'-phosphoadenylyl sulfate + protein tyrosine = adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
show the reaction diagram
mechanism
-
3'-phosphoadenylyl sulfate + protein tyrosine = adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
show the reaction diagram
enzymatic mechanism and structure-function relationships, overview. The catalytic mechanism follows the sequential steps: binding of PAPS, binding of the target protein, sulfate transfer, release of the sulfated target and release of 3',5'-ADP
-
3'-phosphoadenylyl sulfate + protein tyrosine = adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
sulfate group transfer
-
-
-
-
sulfate group transfer
-
tyrosine sulfation
SYSTEMATIC NAME
IUBMB Comments
3'-phosphoadenylyl-sulfate:protein-tyrosine O-sulfotransferase
The tyrosine residues of some specific proteins of rat pheochromocytoma cells act as acceptors.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
AQC1
-
gene name
sulfotransferase, protein (tyrosine)
-
-
-
-
TPST
-
-
-
-
TPST
-
-
TPST
Q6WZB4
-
TPST
O60507
-
TPST
O60704
-
TPST-1
Caenorhabditis elegans N2
-
-
-
TPST-2
-
isoform
TPST1
-
-
TPST1
-
-
tyrosylprotein sulfotransferase
-
-
-
-
tyrosylprotein sulfotransferase
-
-
tyrosylprotein sulfotransferase
-
-
tyrosylprotein sulfotransferase
-
-
tyrosylprotein sulfotransferase
Caenorhabditis elegans N2
-
-
-
tyrosylprotein sulfotransferase
Q6WZB4
-
tyrosylprotein sulfotransferase
-
-
tyrosylprotein sulfotransferase
O60507
-
tyrosylprotein sulfotransferase
O60704
-
tyrosylprotein sulfotransferase
-
-
tyrosylprotein sulfotransferase
-
-
tyrosylprotein sulfotransferase 1
O60507
-
tyrosylprotein sulfotransferase 2
-
-
tyrosylprotein sulfotransferase-1
O60507
-
tyrosylprotein sulfotransferase-1
-
-
tyrosylprotein sulfotransferase-2
-
-
tyrosylprotein sulfotransferase-2
O60704
-
tyrosylprotein sulfotransferase-2
-
-
tyrosylprotein sulfotransferase-A
-
-
CAS REGISTRY NUMBER
COMMENTARY
87588-33-8
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
ecotypes Columbia, C24, Landsberg erecta, and Wassilewskija
-
-
Manually annotated by BRENDA team
Caenorhabditis elegans N2
-
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
isozymes TPST-1 and TPST-2
-
-
Manually annotated by BRENDA team
only 1 isoform
Uniprot
Manually annotated by BRENDA team
2 isoforms: TPST1 and TPST2
-
-
Manually annotated by BRENDA team
isozymes TPST-1 and TPST-2
-
-
Manually annotated by BRENDA team
TPST-1
SwissProt
Manually annotated by BRENDA team
TPST-1; isozyme TPST-1
SwissProt
Manually annotated by BRENDA team
TPST-2; isozyme TPST-2
SwissProt
Manually annotated by BRENDA team
isoforms TPST1 and TPST2
-
-
Manually annotated by BRENDA team
C57BL/6J and grt mice, two isozymes encoded by genes Tpst1 and Tpst2
-
-
Manually annotated by BRENDA team
isozyme TPST1; isozyme TPST2
-
-
Manually annotated by BRENDA team
isozymes TPST-1 and TPST-2
-
-
Manually annotated by BRENDA team
male mice, isozymes TPST-1 and TPST-2
-
-
Manually annotated by BRENDA team
wild-type DW/J mice and mutant grt mice
-
-
Manually annotated by BRENDA team
no activity in prokaryotes
-
-
-
Manually annotated by BRENDA team
no activity in yeast
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
malfunction
-
deficiency in the enzymatic activity of isozyme TPST2 causes growth-retardation in mice, autosomal recessive hypothyroidism, and lifelong female infertility. The isozyme deficiency is also involved in severe thyroid hypogenesis and consequent dwarfism, due to the impairment of the tyrosine sulfation of thyroid-stimulating hormone receptor by mutant TPST2
malfunction
-
knockdown of the enzyme blocks the cuticle localization of ROL-6
malfunction
-
mutation of the enzyme leads to defective maintenance of the root stem cell niche, decreased meristematic activity, and stunted root growth. Mutation of the enzyme also impairs basal- and auxin-induced expression of the Plethora stem cell transcription factor genes
malfunction
Caenorhabditis elegans N2
-
knockdown of the enzyme blocks the cuticle localization of ROL-6
-
metabolism
-
the two independent TPSTs catalyze the tyrosine-O-sulfation, a post-translational modification
physiological function
-
retinal tyrosine O-sulfation is a highly conserved, post-translational modification
physiological function
-
tyrosine sulfation is required for normal pulmonary function at birth, and isozyme TPST-2 is required for normal thyroid gland function
physiological function
-
post-translational sulfation of tyrosine residues occurs in numerous secreted and integral membrane proteins and, in many cases, plays a crucial role in controlling the interactions of these proteins with physiological binding partners as well as invading pathogens
physiological function
-
post-translational sulfation of tyrosine residues occurs in numerous secreted and integral membrane proteins and, in many cases, plays a crucial role in controlling the interactions of these proteins with physiological binding partners as well as invading pathogens, physiological functions of protein sulfations, overview
physiological function
-
post-translational sulfation of tyrosine residues occurs in numerous secreted and integral membrane proteins and, in many cases, plays a crucial role in controlling the interactions of these proteins with physiological binding partners as well as invading pathogens
physiological function
-
the enzyme regulates cuticle organization by promoting the transport of ROL-6 from the trans Golgi network to the cuticle. The sulfation of ROL-6 by TPST-1 is critical for its proper localization
physiological function
-
Arabidopsis tyrosylprotein sulfotransferase acts in the auxin/Plethora (PLT) pathway in regulating postembryonic maintenance of the root stem cell niche by regulating basal- and auxin-induced expression of PLT1 and PLT2. The enzyme also affects local auxin biosynthesis in the root meristem
physiological function
Caenorhabditis elegans N2
-
the enzyme regulates cuticle organization by promoting the transport of ROL-6 from the trans Golgi network to the cuticle. The sulfation of ROL-6 by TPST-1 is critical for its proper localization
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + Ac-YLFSVHWPPLNK(COOSu)A-OH
adenosine 3',5'-bisphosphate + Ac-Y(O-sulfate)LFSVHWPPLNK(COOSu)A-OH
show the reaction diagram
-
an N-succinimidyl carbamate modified peptide substrate
-
-
?
3'-phosphoadenylyl sulfate + Ac-YLFSVHWPPLNKA-OH
adenosine 3',5'-bisphosphate + Ac-Y(O-sulfate)LFSVHWPPLNKA-OH
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + benzyl-Glu-Tyr
adenosine 3',5'-bisphosphate + benzyl-Glu-Tyr-O-sulfate
show the reaction diagram
O60507, O60704
-
-
-
?
3'-phosphoadenylyl sulfate + C3aR tyrosine
adenosine 3',5'-bisphosphate + C3aR tyrosine O-sulfate
show the reaction diagram
-
C3a-anaphylatoxin chemotactic receptor,C3aR. Sulfation sequence motif is HNRCGY174KFGLSSSLDY184PDFY188 GDPLEQGFQDY317Y318NLGQF
-
-
?
3'-phosphoadenylyl sulfate + C5aR tyrosine
adenosine 3',5'-bisphosphate + C5aR tyrosine O-sulfate
show the reaction diagram
-
C5a-anaphylatoxin chemotactic receptor. Sulfation sequence motif is TTPDY11GHY14DDKDTLD
-
-
?
3'-phosphoadenylyl sulfate + CC receptor tyrosine
adenosine 3',5'-bisphosphate + CC receptor tyrosine O-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + CCR5 1-18 peptide
adenosine 3',5'-bisphosphate + sulfated CCR5 1-18peptide
show the reaction diagram
-
substrate is a synthetic human CCR5 peptide MDYQVSSPIYDINYYTSE-NH2. Sulfation pattern: Tyr3 is sulfated first, followed by Tyr14 or Tyr15, and finally Tyr10
-
-
?
3'-phosphoadenylyl sulfate + CCR5 2-18 peptide
adenosine 3',5'-bisphosphate + sulfated CCR5 2-18peptide
show the reaction diagram
-
substrate is a synthetic human CCR5 peptide DYQVSSPIYDINYYTSE-NH2. Sulfation pattern: Tyr14 and Tyr15 are sulfated first, followed by Tyr10, with Tyr3 sulfated last
-
-
?
3'-phosphoadenylyl sulfate + CCR8 1-17 M1A peptide
adenosine 3',5'-bisphosphate + sulfated CCR8 1-17 M1A peptide
show the reaction diagram
-
substrate is a synthetic modified mouse CCR8 peptide ADYTMEPNVTMTDYYPD-NH2. Sulfation pattern: Tyr3 is sulfated first, followed by Tyr14, with Tyr15 last
-
-
?
3'-phosphoadenylyl sulfate + CCR8 1-17 M1A peptide
adenosine 3',5'-bisphosphate + sulfated CCR8 1-17 M1A peptide
show the reaction diagram
-
substrate is a synthetic modified mouse CCR8 peptide ADYTMEPNVTMTDYYPD-NH2. Sulfation pattern: Tyr3 is sulfated first, followed by Tyr14, with Tyr15 last
-
-
?
3'-phosphoadenylyl sulfate + CCR8 2-17 M1A peptide
adenosine 3',5'-bisphosphate + sulfated CCR8 2-17 M1A peptide
show the reaction diagram
-
substrate is a synthetic modified mouse CCR8 peptide DYTMEPNVTMTDYYPD-NH2. Sulfation pattern: Tyr14 is sulfated first, followed by Tyr3 and Tyr15
-
-
?
3'-phosphoadenylyl sulfate + CCR8 2-17 M1A peptide
adenosine 3',5'-bisphosphate + sulfated CCR8 2-17 M1A peptide
show the reaction diagram
-
substrate is a synthetic modified mouse CCR8 peptide DYTMEPNVTMTDYYPD-NH2. Sulfation pattern: Tyr14 is sulfated first, followed by Tyr3 and Tyr15
-
-
?
3'-phosphoadenylyl sulfate + CX3CR1 tyrosine
adenosine 3',5'-bisphosphate + CX3CR1 tyrosine O-sulfate
show the reaction diagram
-
a cytokine receptor for CXCL10/IP-10 and CXCL11/I-TAC, contains two potential tyrosine sulfation sites within the sequence ENFSSSY27DY29GENESDS, a cytokine receptor for CXCL10/IP-10 and CXCL11/I-TAC, contains two potential tyrosine sulfation sites within the sequence ENFSSSY27DY29GENESDS. Determination of sulfation sites by mutational analyis, overview. CX3CR1 has one known chemokine ligand, fractalkine and two tyrosine residues in its N-terminal region, Tyr14 and Tyr22
-
-
?
3'-phosphoadenylyl sulfate + CXC-chemokine receptor 4
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
O60704
CXCR4 signaling is indispensable for embryonic development, CXCR4
-
-
?
3'-phosphoadenylyl sulfate + CXCR4 1-38
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
O60704
CC-chemokine receptor 4 peptide, amino acids 1-38
the enzyme has a strong site preference for sulfation of Tyr-21 over Tyr-7 and Tyr-12, sulfation of Tyr-21 is required for high-affinity interaction with stromal cell-derived factor-1 (SDF-1alpha)
-
?
3'-phosphoadenylyl sulfate + CXCR4 peptide tyrosine
adenosine 3',5'-bisphosphate + CXCR4 peptide tyrosine O-sulfate
show the reaction diagram
-
a cytokine receptor peptide fragment
-
-
?
3'-phosphoadenylyl sulfate + EDYDTTTEFDsYGD
adenosine 3',5'-bisphosphate + EDsYDTTTEFDsYGD
show the reaction diagram
-
G protein-coupled receptor, human chemokine receptor 1, amino acids 8-20
-
-
?
3'-phosphoadenylyl sulfate + EDYDTTTEFDYGD
adenosine 3',5'-bisphosphate + EDYDTTTEFDsYGD
show the reaction diagram
-
G protein-coupled receptor human chemokine receptor 1, amino acids 8-20
-
-
?
3'-phosphoadenylyl sulfate + EEPEYGE
adenosine 3',5'-bisphosphate + EEPEY(O-sulfate)GE
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + ENYSYDLDYYS
adenosine 3',5'-bisphosphate + ENsYSsYDLDsYsYS
show the reaction diagram
-
G protein-coupled receptor human gpr1, amino acids 13-23, sulfation order: first Tyr21, second Tyr22, third Tyr15 or 17, fourth Tyr 15 or 17
-
-
?
3'-phosphoadenylyl sulfate + FSHR tyrosine
adenosine 3',5'-bisphosphate + FSHR tyrosine O-sulfate
show the reaction diagram
-
follicle-stimulating hormone receptor, FSHR. Sulfation sequence motif is F-D/E-Y335
-
-
?
3'-phosphoadenylyl sulfate + glutathione S-transferase-tagged P-selectin glycoprotein ligand-1
adenosine 3',5'-bisphosphate + glutathione S-transferase-tagged P-selectin glycoprotein ligand-1 tyrosine O-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + human anionic trypsinogen tyrosine
adenosine 3',5'-bisphosphate + trypsinogen tyrosine O-sulfate
show the reaction diagram
-
i.e. serine protease 2, sulfation of Tyr154 facilitated by a unique sequence context which is characteristically found in primate trypsinogens
-
-
?
3'-phosphoadenylyl sulfate + LHR/CGR tyrosine
adenosine 3',5'-bisphosphate + LHR/CGR tyrosine O-sulfate
show the reaction diagram
-
luteinising hormone receptor, LHR/chorionic gonadotropin receptor, CGR. Sulfation sequence motif is Y385-D/E-Y
-
-
?
3'-phosphoadenylyl sulfate + Mfge8 protein tyrosine
adenosine 3',5'-bisphosphate + Mfge8 protein tyrosine O-sulfate
show the reaction diagram
-
the long isoform of Mfge8, substrate of isozyme TPST-2, not isozyme TPST-1, mutational analysis, overview, Mfge8 is expressed on mouse sperm, and recombinantly in Escherichia coli strain BL21(DE3) as His-tagged protein
-
-
?
3'-phosphoadenylyl sulfate + P-selectin glycoprotein ligand-1
adenosine 3',5'-bisphosphate + P-selectin glycoprotein ligand-1 tyrosine O-sulfate
show the reaction diagram
-
i.e. ATEYEYLDYDFL
-
-
?
3'-phosphoadenylyl sulfate + P-selectin glycoprotein ligand-1 tyrosine
adenosine 3',5'-bisphosphate + P-selectin glycoprotein ligand-1 tyrosine O-sulfate
show the reaction diagram
-
sulfoTyr plays a functional role is P-selectin glycoprotein ligand-1, which is involved in leukocyte-mediated inflammation, tyrosine sulfation of the amino-terminal region, structural basis of sulfotyrosine recognition, overview
-
-
?
3'-phosphoadenylyl sulfate + peptide C4P5Y3
?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + poly-Glu6Ala3Tyr1 peptide
adenosine 3',5'-bisphosphate + poly-Glu6Ala3Tyrsulfate peptide
show the reaction diagram
-
-
-
-
-
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
show the reaction diagram
O60507
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
show the reaction diagram
O60507, O60704
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
show the reaction diagram
O60704
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
show the reaction diagram
-
tyrosine sulfonation of TSHR by TPST is crucial for thyrotropin signaling, overview
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
show the reaction diagram
O60507, O60704
high activity, isozyme TPST-2
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
show the reaction diagram
O60507, O60704
low activity, isozyme TPST-1
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
show the reaction diagram
-
TPST is responsible for the sulfation of a variety of secretory and membrane proteins
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
show the reaction diagram
-
tyrosine O-sulfation is a key post-translational modification that regulates protein-protein interactions in extracellular space
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
show the reaction diagram
-
CC-chemokine receptor 8 nonapeptide, sY15CCR8 I20G mutant
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine O-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine O-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine O-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine O-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine O-sulfate
show the reaction diagram
-
sulfation of protein in the retina, identification of protein substrates, sulfation pattern, overview
-
-
?
3'-phosphoadenylyl sulfate + PSGL-1
adenosine 3',5'-bisphosphate + PSGL-1 sulfate
show the reaction diagram
O60507, O60704
-
-
-
?
3'-phosphoadenylyl sulfate + PSGL-1 protein
adenosine 3',5'-bisphosphate + PSGL-1 protein tyrosine-O-sulfate
show the reaction diagram
-
i.e. ATEYEYLDYDFL
-
-
?
3'-phosphoadenylyl sulfate + QATEYEYLDYDFLPE
adenosine 3',5'-bisphosphate + QATEsYEsYLDsYDFLPE
show the reaction diagram
-
human PSGL-1, amino acids 42-56, sulfation order: first Tyr51, second Tyr46 or 48, third Tyr46 or 48
-
-
?
3'-phosphoadenylyl sulfate + QATEYEYLDYDFLPE-NH2
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
O60704
P-selectin glycoprotein ligand-1, amino acids 1-15
-
-
?
3'-phosphoadenylyl sulfate + RNase 9 protein tyrosine
adenosine 3',5'-bisphosphate + RNase 9 protein tyrosine O-sulfate
show the reaction diagram
-
substrate of isozyme TPST-2, not isozyme TPST-1, mutational analysis, overview, RNase 9 is a catalytically inactive RNase A family member of unknown function and is expressed only in the epididymis after onset of sexual maturity
-
-
?
3'-phosphoadenylyl sulfate + ROL-6 protein tyrosine
adenosine 3',5'-bisphosphate + ROL-6 protein tyrosine-O-sulfate
show the reaction diagram
Caenorhabditis elegans, Caenorhabditis elegans N2
-
the enzyme sulfates three tyrosine residues of ROL-6 protein in vitro
-
-
?
3'-phosphoadenylyl sulfate + S1P1R tyrosine
adenosine 3',5'-bisphosphate + S1P1R tyrosine O-sulfate
show the reaction diagram
-
type 1 Sphingosine 1-phosphate receptor, S1P1R. Sulfation sequence motif is D-Y19-V/G/-N-Y22-D
-
-
?
3'-phosphoadenylyl sulfate + statherin
adenosine 3',5'-bisphosphate + statherin O-sulfate
show the reaction diagram
-
TPST is responsible for the sulfation of a variety of secretory and membrane proteins, e.g. of the salivary secretory protein, statherin, determination of sulfation on a tyrosyl residue, probably in the sequence E-Q-P-L-Y, residues 26-30
-
-
?
3'-phosphoadenylyl sulfate + statherin
adenosine 3',5'-bisphosphate + statherin-O-sulfate
show the reaction diagram
-
a 43-residue polypeptide, which is tyrosine-rich and contains 7 tyrosine residues
-
-
?
3'-phosphoadenylyl sulfate + SYGDEYPDYLD
adenosine 3',5'-bisphosphate + SsYGDEsYPDsYLD
show the reaction diagram
-
G protein-coupled receptor human dez, amino acids 13-23, sulfation order: first Tyr21, second Tyr18, third Tyr14
-
-
?
3'-phosphoadenylyl sulfate + trypsinogen tyrosine
adenosine 3',5'-bisphosphate + trypsinogen tyrosine O-sulfate
show the reaction diagram
-
sulfation of Tyr154 with Asp as important determinant. Mutation D153N, a naturally occuring polymorphisms in African population, results in a complete loss of trypsinogen sulfation, while mutation of Gly151 and Pro155 of the trypsinogen has no effect on enzyme activity. Genotyping of different populations for the substrate polymorphism, overview
-
-
?
3'-phosphoadenylyl sulfate + TSH receptor
adenosine 3',5'-bisphosphate + TSH receptor O-sulfate
show the reaction diagram
-
-, tyrosine sulfonation of TSHR by TPST2 is crucial for thyrotropin signaling, TPST1 and TPST2 mutant H266Q are incapable of mediating the signal transduction, overview
-
-
?
3'-phosphoadenylyl sulfate + TSHR tyrosine
adenosine 3',5'-bisphosphate + TSHR tyrosine O-sulfate
show the reaction diagram
-
thyroid-stimulating hormone receptor, TSHR. Sulfation sequence motif is Y385-D/E-Y
-
-
?
3'-phosphoadenylyl sulfate + TTFFDsYDYGA
adenosine 3',5'-bisphosphate + TTFFDsYDsYGA
show the reaction diagram
-
G protein-coupled receptor, human chemokine receptor 2, amino acids 21-30
-
-
?
3'-phosphoadenylyl sulfate + TTFFDYDsYGA
adenosine 3',5'-bisphosphate + TTFFDsYDsYGA
show the reaction diagram
-
G protein-coupled receptor, human chemokine receptor 2, amino acids 21-30
-
-
?
3'-phosphoadenylyl sulfate + TTFFDYDYGA
adenosine 3',5'-bisphosphate + TTFFDsYDYGA
show the reaction diagram
-
G protein-coupled receptor, human chemokine receptor 2, amino acids 21-30
-
-
?
3'-phosphoadenylyl sulfate + TTFFDYDYGA
adenosine 3',5'-bisphosphate + TTFFDYDsYGA
show the reaction diagram
-
G protein-coupled receptor, human chemokine receptor 2, amino acids 21-30
-
-
?
3'-phosphoadenylyl sulfate + Tyr-Tyr-Tyr
adenosine 3',5'-bisphosphate + Tyr-Tyr-Tyr-O-sulfate
show the reaction diagram
O60507, O60704
-
-
-
?
3'-phosphoadenylyl sulfate + vitronectin peptide
adenosine 3',5'-bisphosphate + vitronectin-O-sulfate
show the reaction diagram
-
amino acids 75-91, Y(SO3H)TVY(SO3H)DDGEEKNDATVHE
-
-
?
3'-phosphoadenylyl sulfate + VTDsYsYYPDI
adenosine 3',5'-bisphosphate + VTDsYsYsYPDI
show the reaction diagram
-
Tyr16-sulfated G protein-coupled receptor CCR8, amino acids 12-20
-
-
?
3'-phosphoadenylyl sulfate + VTDsYYsYPDI
adenosine 3',5'-bisphosphate + VTDsYsYsYPDI
show the reaction diagram
-
CC-chemokine receptor 8 nonapeptide, sY15CCR8
-
-
?
3'-phosphoadenylyl sulfate + VTDsYYYPDG
adenosine 3',5'-bisphosphate + VTDsYYsYPDI
show the reaction diagram
-
CC-chemokine receptor 8 nonapeptide, sY15sY17CCR8
-
-
?
3'-phosphoadenylyl sulfate + VTDsYYYPDI
adenosine 3',5'-bisphosphate + VTDsYYsYPDI
show the reaction diagram
-
-
-
-
ir
3'-phosphoadenylyl sulfate + VTDsYYYPDI
adenosine 3',5'-bisphosphate + VTDsYYsYPDI
show the reaction diagram
-
CC-chemokine receptor 8 nonapeptide, amino acids 12-20
-
-
?
3'-phosphoadenylyl sulfate + VTDYsYYPDI
adenosine 3',5'-bisphosphate + VTDsYsYYPDI
show the reaction diagram
-
Tyr16-sulfated G protein-coupled receptor CCR8, amino acids 12-20
-
-
?
3'-phosphoadenylyl sulfate + VTDYYsYPDI
adenosine 3',5'-bisphosphate + VTDsYYsYPDI
show the reaction diagram
-
both isoenzymes sequentially sulfate the CCR8 peptide such that Tyr15 is sulfated first followed by Tyr17 sulfation
-
-
?
3'-phosphoadenylyl sulfate + VTDYYYPDI
adenosine 3',5'-bisphosphate + VTDsYYYPDI
show the reaction diagram
-
-
-
-
ir
3'-phosphoadenylyl sulfate + VTDYYYPDI
adenosine 3',5'-bisphosphate + VTDsYYYPDI
show the reaction diagram
-
non CC-chemokine receptor 8
-
-
?
3'-phosphoadenylyl sulfate + VTDYYYPDI
adenosine 3',5'-bisphosphate + VTDYYsYPDI
show the reaction diagram
-
a peptide modeled on the N-terminal extracellular domain of human CCR8, amino acids 12-20
-
-
?
3'-phosphoadenylyl-sulfate + ATEFEFLDYDFL
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-, Q6WZB4
-
-
-
?
3'-phosphoadenylyl-sulfate + ATEFEYLDYDFL
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-, Q6WZB4
-
-
-
?
3'-phosphoadenylyl-sulfate + ATEYEFLDYDFL
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-, Q6WZB4
-
-
-
?
3'-phosphoadenylyl-sulfate + ATEYEYLDYDFL
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-, Q6WZB4
-
-
-
?
3'-phosphoadenylyl-sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
show the reaction diagram
-
TPST-A is involved in the cuticle formation in the nematode. TPST-A RNAi significantly decreases the dityrosine level in the worms, raising the possibility that the sulfation process may be a pre-requisite for the collagen tyrosine cross-linking
-
-
?
3'-phosphoadenylylsulfate + acidic amino acid polymer EAY
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
i.e. poly-EAY or Glu6,Ala3,Tyr1, model substrate
-
-
?
3'-phosphoadenylylsulfate + acidic amino acid polymer EAY
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
i.e. poly-EAY or Glu6,Ala3,Tyr1, model substrate
-
-
?
3'-phosphoadenylylsulfate + acidic amino acid polymer EAY
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
i.e. poly-EAY or Glu6,Ala3,Tyr1, model substrate
-
-
?
3'-phosphoadenylylsulfate + acidic amino acid polymer EAY
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
i.e. poly-EAY or Glu6,Ala3,Tyr1, model substrate
-
-
?
3'-phosphoadenylylsulfate + acidic polypeptide tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + acidic polypeptide tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
show the reaction diagram
Q9VYB7
dominant characteristic is that there are generally between 3 and 4 acidic amino acids within the +/-5 residues of the sulfotyrosine
-
-
?
3'-phosphoadenylylsulfate + acidic polypeptide tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
show the reaction diagram
-
dominant characteristic is that there are generally between 3 and 4 acidic amino acids within the +/-5 residues of the sulfotyrosine
-
-
?
3'-phosphoadenylylsulfate + acidic polypeptide tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
show the reaction diagram
-
polypeptides from PC12 cells, referred to as p113, p105, p86 and p84, according to their average MW, specifically sulfates Tyr-residues adjacent to acidic amino acids
-
?
3'-phosphoadenylylsulfate + acidic polypeptide tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
show the reaction diagram
-
specifically sulfates Tyr-residues adjacent to acidic amino acids
-
-
?
3'-phosphoadenylylsulfate + alpha-tubulin
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
peptide PKV
-
-
?
3'-phosphoadenylylsulfate + C-terminal peptide fragments of complement component C4
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + C4 alpha chain
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
more efficiently sulphated by TPST1 than TPST2
-
-
?
3'-phosphoadenylylsulfate + DYIYTQDVK
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
PP-PSK-(79-87), truncated sequence of preprophytosulfokinase, poor substrate
-
-
?
3'-phosphoadenylylsulfate + DYQVSSPIYDINYYTSE
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
amino acids 2-18 derived from peptide CC-chemokine receptor 5, final product has four sulfotyrosine residues, sulfation occurs in the following order: Tyr13, Tyr14, Tyr9, Tyr2
-
-
?
3'-phosphoadenylylsulfate + EANEDFEDYEFDE
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
i.e. P395A
-
-
?
3'-phosphoadenylylsulfate + EEFHTDYIYTQDVK
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
PP-PSK-(74-87), truncated sequence of preprophytosulfokinase
-
-
?
3'-phosphoadenylylsulfate + FHTDYIYTQDVK
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
PP-PSK-(76-87), truncated sequence of preprophytosulfokinase, poor substrate
-
-
?
3'-phosphoadenylylsulfate + heparin cofactor II
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
more efficiently sulphated by TPST1 than TPST2
-
-
?
3'-phosphoadenylylsulfate + KKE(EPEYGE)2
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + KKE(EPEYGE)3
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + KKEEPEYGE
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + KKEEPEYGE(EPEFGE)2
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + KKSAEDYEYPSMG
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
-
3'-phosphoadenylylsulfate + KKSAEDYEYPSMG
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + KKSAEDYEYPSMG
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
-
3'-phosphoadenylylsulfate + KKSAEDYQYPSMG
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + KKSAENYEYPSMG
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + KKSAENYQYPSMG
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + PKG
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + preprocholecystokinin
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
CCK-(107-115) corresponding to the carboxy-terminal sulfation sites of the protein
-
-
?
3'-phosphoadenylylsulfate + protein tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + protein tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
show the reaction diagram
-
involved in post-translational processing of specific PC12 cell proteins
-
-
?
3'-phosphoadenylylsulfate + protein tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
show the reaction diagram
-
enzyme of trans-Golgi network catalyzing post-translational sulfation of a variety of secretory and membrane proteins
-
-
?
3'-phosphoadenylylsulfate + protein tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
show the reaction diagram
-
post-translational modification of biologically active peptides and proteins
-
-
?
3'-phosphoadenylylsulfate + protein tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
show the reaction diagram
-
post-translational modification of biologically active peptides and proteins
-
-
?
3'-phosphoadenylylsulfate + protein tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
show the reaction diagram
-
post-translational modification of biologically active peptides and proteins
-
-
?
3'-phosphoadenylylsulfate + QATEYEYLDYDFLPE
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
derived from N-terminal residues of the mature leukocyte adhesion molecule P-selectin glycoprotein ligand 1
-
-
?
3'-phosphoadenylylsulfate + QATEYEYLDYDFLPEC
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
O70281
derived from N-terminal residues of the mature leukocyte adhesion molecule P-selectin glycoprotein ligand 1
-
-
?
3'-phosphoadenylylsulfate + QATEYEYLDYDFLPEC
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
O60507
derived from N-terminal residues of the mature leukocyte adhesion molecule P-selectin glycoprotein ligand 1
-
-
?
3'-phosphoadenylylsulfate + QATEYEYLDYDFLPEC
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
derived from N-terminal residues of the mature leukocyte adhesion molecule P-selectin glycoprotein ligand 1
-
-
?
3'-phosphoadenylylsulfate + recombinant hirudin variants
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
rHV2 and Hir-(57-65) corresponding to the nine C-terminal amino acids of hirudin
-
-
?
3'-phosphoadenylylsulfate + recombinant hirudin variants
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
HV2, previously referred to as HV2,Lys-47, or rHV-1
-
-
?
3'-phosphoadenylylsulfate + recombinant hirudin variants
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
sulfated at physiological sulfation site: Tyr-63
-
-
?
3'-phosphoadenylylsulfate + recombinant hirudin variants
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
C-terminal fragment Hir-(54-65), higher activity for those peptides with aspartyl residues on N-terminal side of Tyr-residue compared with glutamyl residues
-
-
?
3'-phosphoadenylylsulfate + synthetic peptides
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + synthetic peptides
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
highly site-specific
-
-
?
3'-phosphoadenylylsulfate + synthetic peptides
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
peptides modelled after known or putative Tyr-sulfation sites of chromogranin B, i.e. cholecystokinin precursor, peptides CCK-1 and variants, secretogranin I, peptides SgI-1 to SgI-4, vitronectin modeled after phosphorylation site of alpha-tubulin or autophosphorylation site of pp60src, pp60v-src(Tyr-416)
-
-
?
3'-phosphoadenylylsulfate + synthetic peptides
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
structure based on sequences surrounding the sulfated Tyr of naturally sulfated proteins
-
-
?
3'-phosphoadenylylsulfate + synthetic peptides
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
structure based on sequences surrounding the sulfated Tyr of naturally sulfated proteins
-
-
?
3'-phosphoadenylylsulfate + tert-butoxycarbonylcholecystokinin octapeptide
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
BOC-CCK
-
-
?
3'-phosphoadenylylsulfate + WEEFHTDYIYTQDVK
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
PP-PSK-(73-87), truncated sequence of preprophytosulfokinase
-
-
?
additional information
?
-
-
structural determinants for substrate specificity
-
-
-
additional information
?
-
-
missense mutation of Tpst2 leads to the autosomal recessive, fetal-onset, severe thyroid hypoplasia phenotype related to TSH hyporesponsiveness in growth-retarded mice, overview
-
-
-
additional information
?
-
-
protein tyrosine O-sulfation is a post-translational modification mediated by the Golgi tyrosylprotein sulfotransferase, TPST1, that catalyzes the transfer of sulfate to tyrosine residues in secreted and transmembrane proteins, tyrosine sulfation plays a role in protein-protein interactions, overview, protein tyrosine O-sulfation is a post-translational modification mediated by the Golgi tyrosylprotein sulfotransferase, TPST2, that catalyzes the transfer of sulfate to tyrosine residues in secreted and transmembrane proteins, tyrosine sulfation plays a role in protein-protein interactions, overview
-
-
-
additional information
?
-
-
TPST-2 catalyzes post-translational protein tyrosine O-sulfation, TPST-2, but not TPST-1, deficiency causes male infertility
-
-
-
additional information
?
-
O60507, O60704
activity with peptides encompassing tyrosine sulfation sites of chemokine receptors, CCR2, CCR3, CCR5, CCR8, cholecystokinin CCK, and complement C4, overview
-
-
-
additional information
?
-
-
isozyme Tpst1 shows differences in the macromolecular substrate specificities, overview, isozyme Tpst2 shows differences in the macromolecular substrate specificities, overview
-
-
-
additional information
?
-
-
sulfation patterns, overview, enzyme activity with CCR receptors, overview
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + C3aR tyrosine
adenosine 3',5'-bisphosphate + C3aR tyrosine O-sulfate
show the reaction diagram
-
C3a-anaphylatoxin chemotactic receptor,C3aR. Sulfation sequence motif is HNRCGY174KFGLSSSLDY184PDFY188 GDPLEQGFQDY317Y318NLGQF
-
-
?
3'-phosphoadenylyl sulfate + C5aR tyrosine
adenosine 3',5'-bisphosphate + C5aR tyrosine O-sulfate
show the reaction diagram
-
C5a-anaphylatoxin chemotactic receptor. Sulfation sequence motif is TTPDY11GHY14DDKDTLD
-
-
?
3'-phosphoadenylyl sulfate + CC receptor tyrosine
adenosine 3',5'-bisphosphate + CC receptor tyrosine O-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + CX3CR1 tyrosine
adenosine 3',5'-bisphosphate + CX3CR1 tyrosine O-sulfate
show the reaction diagram
-
a cytokine receptor for CXCL10/IP-10 and CXCL11/I-TAC, contains two potential tyrosine sulfation sites within the sequence ENFSSSY27DY29GENESDS
-
-
?
3'-phosphoadenylyl sulfate + CXC-chemokine receptor 4
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
O60704
CXCR4 signaling is indispensable for embryonic development
-
-
?
3'-phosphoadenylyl sulfate + FSHR tyrosine
adenosine 3',5'-bisphosphate + FSHR tyrosine O-sulfate
show the reaction diagram
-
follicle-stimulating hormone receptor, FSHR. Sulfation sequence motif is F-D/E-Y335
-
-
?
3'-phosphoadenylyl sulfate + glutathione S-transferase-tagged P-selectin glycoprotein ligand-1
adenosine 3',5'-bisphosphate + glutathione S-transferase-tagged P-selectin glycoprotein ligand-1 tyrosine O-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + LHR/CGR tyrosine
adenosine 3',5'-bisphosphate + LHR/CGR tyrosine O-sulfate
show the reaction diagram
-
luteinising hormone receptor, LHR/chorionic gonadotropin receptor, CGR. Sulfation sequence motif is Y385-D/E-Y
-
-
?
3'-phosphoadenylyl sulfate + Mfge8 protein tyrosine
adenosine 3',5'-bisphosphate + Mfge8 protein tyrosine O-sulfate
show the reaction diagram
-
the long isoform of Mfge8, substrate of isozyme TPST-2, not isozyme TPST-1, mutational analysis, overview
-
-
?
3'-phosphoadenylyl sulfate + P-selectin glycoprotein ligand-1
adenosine 3',5'-bisphosphate + P-selectin glycoprotein ligand-1 tyrosine O-sulfate
show the reaction diagram
-
i.e. ATEYEYLDYDFL
-
-
?
3'-phosphoadenylyl sulfate + P-selectin glycoprotein ligand-1 tyrosine
adenosine 3',5'-bisphosphate + P-selectin glycoprotein ligand-1 tyrosine O-sulfate
show the reaction diagram
-
sulfoTyr plays a functional role is P-selectin glycoprotein ligand-1, which is involved in leukocyte-mediated inflammation
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
show the reaction diagram
O60507
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
show the reaction diagram
O60507, O60704
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
show the reaction diagram
O60704
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
show the reaction diagram
-
tyrosine sulfonation of TSHR by TPST is crucial for thyrotropin signaling, overview
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
show the reaction diagram
-
TPST is responsible for the sulfation of a variety of secretory and membrane proteins
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
show the reaction diagram
-
tyrosine O-sulfation is a key post-translational modification that regulates protein-protein interactions in extracellular space
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine O-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine O-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine O-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine O-sulfate
show the reaction diagram
-
sulfation of protein in the retina, identification of protein substrates, sulfation pattern, overview
-
-
?
3'-phosphoadenylyl sulfate + PSGL-1 protein
adenosine 3',5'-bisphosphate + PSGL-1 protein tyrosine-O-sulfate
show the reaction diagram
-
i.e. ATEYEYLDYDFL
-
-
?
3'-phosphoadenylyl sulfate + RNase 9 protein tyrosine
adenosine 3',5'-bisphosphate + RNase 9 protein tyrosine O-sulfate
show the reaction diagram
-
substrate of isozyme TPST-2, not isozyme TPST-1, mutational analysis, overview
-
-
?
3'-phosphoadenylyl sulfate + ROL-6 protein tyrosine
adenosine 3',5'-bisphosphate + ROL-6 protein tyrosine-O-sulfate
show the reaction diagram
Caenorhabditis elegans, Caenorhabditis elegans N2
-
the enzyme sulfates three tyrosine residues of ROL-6 protein in vitro
-
-
?
3'-phosphoadenylyl sulfate + S1P1R tyrosine
adenosine 3',5'-bisphosphate + S1P1R tyrosine O-sulfate
show the reaction diagram
-
type 1 Sphingosine 1-phosphate receptor, S1P1R. Sulfation sequence motif is D-Y19-V/G/-N-Y22-D
-
-
?
3'-phosphoadenylyl sulfate + statherin
adenosine 3',5'-bisphosphate + statherin O-sulfate
show the reaction diagram
-
TPST is responsible for the sulfation of a variety of secretory and membrane proteins, e.g. of the salivary secretory protein, statherin
-
-
?
3'-phosphoadenylyl sulfate + trypsinogen tyrosine
adenosine 3',5'-bisphosphate + trypsinogen tyrosine O-sulfate
show the reaction diagram
-
sulfation of Tyr154 with Asp as important determinant. Mutation D153N, a naturally occuring polymorphisms in African population, results in a complete loss of trypsinogen sulfation, while mutation of Gly151 and Pro155 of the trypsinogen has no effect on enzyme activity. Genotyping of different populations for the substrate polymorphism, overview
-
-
?
3'-phosphoadenylyl sulfate + TSH receptor
adenosine 3',5'-bisphosphate + TSH receptor O-sulfate
show the reaction diagram
-
tyrosine sulfonation of TSHR by TPST2 is crucial for thyrotropin signaling, TPST1 and TPST2 mutant H266Q are incapable of mediating the signal transduction, overview
-
-
?
3'-phosphoadenylyl sulfate + TSHR tyrosine
adenosine 3',5'-bisphosphate + TSHR tyrosine O-sulfate
show the reaction diagram
-
thyroid-stimulating hormone receptor, TSHR. Sulfation sequence motif is Y385-D/E-Y
-
-
?
3'-phosphoadenylyl sulfate + VTDsYYYPDI
adenosine 3',5'-bisphosphate + VTDsYYsYPDI
show the reaction diagram
-
-
-
-
ir
3'-phosphoadenylyl sulfate + VTDYYYPDI
adenosine 3',5'-bisphosphate + VTDsYYYPDI
show the reaction diagram
-
-
-
-
ir
3'-phosphoadenylyl-sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
show the reaction diagram
-
TPST-A is involved in the cuticle formation in the nematode. TPST-A RNAi significantly decreases the dityrosine level in the worms, raising the possibility that the sulfation process may be a pre-requisite for the collagen tyrosine cross-linking
-
-
?
3'-phosphoadenylylsulfate + protein tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + protein tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
show the reaction diagram
-
involved in post-translational processing of specific PC12 cell proteins
-
-
?
3'-phosphoadenylylsulfate + protein tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
show the reaction diagram
-
enzyme of trans-Golgi network catalyzing post-translational sulfation of a variety of secretory and membrane proteins
-
-
?
3'-phosphoadenylylsulfate + protein tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
show the reaction diagram
-
post-translational modification of biologically active peptides and proteins
-
-
?
3'-phosphoadenylylsulfate + protein tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
show the reaction diagram
-
post-translational modification of biologically active peptides and proteins
-
-
?
3'-phosphoadenylylsulfate + protein tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
show the reaction diagram
-
post-translational modification of biologically active peptides and proteins
-
-
?
additional information
?
-
-
missense mutation of Tpst2 leads to the autosomal recessive, fetal-onset, severe thyroid hypoplasia phenotype related to TSH hyporesponsiveness in growth-retarded mice, overview
-
-
-
additional information
?
-
-
protein tyrosine O-sulfation is a post-translational modification mediated by the Golgi tyrosylprotein sulfotransferase, TPST1, that catalyzes the transfer of sulfate to tyrosine residues in secreted and transmembrane proteins, tyrosine sulfation plays a role in protein-protein interactions, overview, protein tyrosine O-sulfation is a post-translational modification mediated by the Golgi tyrosylprotein sulfotransferase, TPST2, that catalyzes the transfer of sulfate to tyrosine residues in secreted and transmembrane proteins, tyrosine sulfation plays a role in protein-protein interactions, overview
-
-
-
additional information
?
-
-
TPST-2 catalyzes post-translational protein tyrosine O-sulfation, TPST-2, but not TPST-1, deficiency causes male infertility
-
-
-
additional information
?
-
-
sulfation patterns, overview
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ca2+
-
activation by 10 mM divalent cations in decreasing order of efficiency: Mn2+, Ca2+, Cu2+, Mg2+, Zn2+
Co2+
-
25% of the stimulation with Mn2+, 20 mM
Co2+
-, Q6WZB4
10 mM, stimulates
Co2+
-
20 mM, stimulates
Cu2+
-
activation by 10 mM divalent cations in decreasing order of efficiency: Mn2+, Ca2+, Cu2+, Mg2+, Zn2+
Divalent cations
-
requirement
Mg2+
-
requirement
Mg2+
-
requirement; requirement for sulfation of tert-butoxycarbonylcholecystokinin
Mg2+
-
1.2fold at 5 mM
Mg2+
-
activation by 10 mM divalent cations in decreasing order of efficiency: Mn2+, Ca2+, Cu2+, Mg2+, Zn2+
Mg2+
-
exhibits around 50% of the stimulation attained with Mn2+
Mg2+
O60507, O60704
activates isozyme TPST-2
Mn2+
-
requirement
Mn2+
-
20 mM; inhibits above 30 mM; requirement
Mn2+
-
5 mM; activation
Mn2+
-
activation
Mn2+
-
30 mM; requirement
Mn2+
-
requirement
Mn2+
-
activation by 10 mM divalent cations in decreasing order of efficiency: Mn2+, Ca2+, Cu2+, Mg2+, Zn2+
Mn2+
-
optimal concentration: 20 mM
Mn2+
-
20 mM; requirement
Mn2+
-, Q6WZB4
20 mM, 2.5fold stimulation
Mn2+
O60507, O60704
activates isozymes TPST-1
Mn2+
-
activates, optimal at 20 mM, not essential
NaCl
-
activation, sulfation of tert-butoxycarbonylcholecystokinin
NaF
-
increases product formation 10fold by preventing degradation of 3'-phosphoadenylylsulfate
Zn2+
-
activation by 10 mM divalent cations in decreasing order of efficiency: Mn2+, Ca2+, Cu2+, Mg2+, Zn2+
MnCl2
-
required, maximal activity at 20 mM
additional information
-
no activation by Ca2+, Cd2+, Cu2+ or Zn2+; no requirement of Mg2+
additional information
-
no requirement for Co2+
additional information
-
Mg2+ cannot replace Mn2+
additional information
O60507, O60704
no activation of isozyme TPST-1 by Mg2+; no activation of isozyme TPST-2 by Mn2+
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2,6-Dichloro-4-nitrophenol
-
excess concentrations, solubilized enzyme
2,6-Dichloro-4-nitrophenol
-
weak
3'-phosphoadenylyl sulfate
-
-
5'-AMP
-
inhibits about 50% at 2 mM
Acidic amino acid polymer
-
excess concentrations
-
adenosine 3',5'-bisphosphate
-
-
adenosine 3',5'-bisphosphate
-
product inhibition
Ca2+
-, Q6WZB4
-
Ca2+
-
inhibits both isozymes
Cd2+
-, Q6WZB4
-
Cu2+
-, Q6WZB4
10 mM, complete inhibition
dithiothreitol
-
-
Erythrosphingosine
-
less effective than sphingosine
Fe2+
-, Q6WZB4
10 mM, complete inhibition
-
Hg2+
-, Q6WZB4
10 mM, complete inhibition
Lubrol Px
-
3fold stimulation at 0.1% w/v, weak inhibition above 0.1% w/v
N-ethylmaleimide
-
-
N-ethylmaleimide
-
1 mM
NaCl
-
above 0.2 M, solubilized enzyme
NaCl
-
above 100 mM, gastric enzyme
Pb2+
-, Q6WZB4
-
phosphatidylcholine
-
weak
Poly-EAY
-
substrate inhibition, above 0.01 mM
Poly-EAY
-
substrate inhibition, above 0.002 mM
psychosine
-
almost complete inhibition
rhreosphingosine
-
-
-
sphingomyelin
-
weak
sphingosine
-
50% inhibition at 0.15 mM, kinetics, enhanced by phosphatidylcholine or sphingomyelin. Phosphatidylinositol, phosphatidylserine or oleic acid reverses
Stearylamine
-
-
Triton X-100
-
inhibits about 50% at 0.25%
Zn2+
-, Q6WZB4
10 mM, complete inhibition
Mn2+
-
above 30 mM, activation below
additional information
-
no inhibition by Glu, Asp, Gln, Asn, Ser
-
additional information
-
Mn2+ presence in culture medium can suppress enzyme activity, but the suppression does not result from direct inhibition
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
5'-AMP
-
increases product formation 10fold by preventing degradation of 3'-phosphoadenylylsulfate
5'-AMP
-
optimal concentration 2 mM
6-keto-prostaglandin F1alpha
-
23% activation at 0.1 mM
CHAPS
-
activation, 0.5% w/v
dithiothreitol
-
1 mM
Lubrol Px
-
3fold stimulation at 0.1% w/v, weak inhibition above 0.1% w/v; activation
Lubrol Px
-
activation
lysophosphatidylcholine
-
slight activation, 0.2 mM
Mg2+
-
strongly stimulates isozyme TPST-2, but not TPST-1
Nonidet P-40
-
activation, 0.5% w/v
Nonidet P-40
-
6fold stimulation at 0.5%
oleic acid
-
activation, 0.2 mM
phosphatidylinositol
-
slight activation, 0.2 mM
phosphatidylserine
-
slight activation, 0.2 mM
prostaglandin E2
-
1.5fold stimulation at 0.1 mM, half maximal stimulation at 0.05 mM
prostaglandin F2alpha
-
22% activation at 0.1 mM
sodium dodecylsulfate
-
2fold stimulation at 0.1%
Sofalcone
-
55% stimulation
Triton X-100
-
microsomal membrane-bound enzyme, not solubilized enzyme; requirement; sulfation of poly-EAY, but not of tert-butoxycarbonylcholecystokinin
Triton X-100
-
activation
Triton X-100
-
activation
Triton X-100
-
6fold stimulation at 0.5%
Tween 20
-
activation, 0.5% w/v
Tween 20
-
6fold stimulation at 0.5%
Tween 80
-
activation, 0.5% w/v
Mn2+
-
strong activation of both isozymes
additional information
-
no activation by octyl glucoside
-
additional information
-
no activation by arachidonic acid
-
additional information
-
stimulation by ethanol involves increased TPST level rather than change in affinity for its substrates
-
additional information
-
no stimulation by 5'-AMP, NaF and Triton X-100
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0021
-
3'-phosphoadenylyl sulfate
-
using glutathione S-transferase-tagged P-selectin glycoprotein ligand-1 as cosubstrate, in 50 mM MES buffer (pH 6.5), 5 mM 2-mercaptoethanol, at 37C
0.0048
-
3'-phosphoadenylyl sulfate
-
with VTDsYYYPDI as cosubstrate, in 20 mM MOPS, at pH 7.5, 100 mM NaCl, at 30C
0.0087
-
3'-phosphoadenylyl sulfate
-
with VTDYYYPDI as cosubstrate, in 20 mM MOPS, at pH 7.5, 100 mM NaCl, at 30C
0.016
-
3'-phosphoadenylyl sulfate
-
using P-selectin glycoprotein ligand-1 as cosubstrate, in 50 mM MES buffer (pH 6.5), 5 mM 2-mercaptoethanol, at 37C
0.5
-
3'-phosphoadenylyl sulfate
-
monosulfation of non CCR8
0.54
-
3'-phosphoadenylyl sulfate
-
monosulfation of non CCR8, mixture of TPST-1 and TPST-2 1:1; monosulfation of non CCR8, mixture of TPST-1 and TPST-2 1:1
0.59
-
3'-phosphoadenylyl sulfate
-
monosulfation of non CCR8
0.00025
-
3'-phosphoadenylylsulfate
-
pH 6.8, 37C
0.0014
-
3'-phosphoadenylylsulfate
-
-
0.0019
0.002
3'-phosphoadenylylsulfate
-
+ peptide CCKI
0.0019
0.002
3'-phosphoadenylylsulfate
-
+ peptide CCKI or peptide EAY
0.0083
-
3'-phosphoadenylylsulfate
-
-
0.009
0.017
3'-phosphoadenylylsulfate
-
pH 6.8, 37C, rat liver after chronic alcohol feeding
0.01
0.0125
3'-phosphoadenylylsulfate
-
pH 6.8, 37C, rat liver, control group without chronic alcohol feeding
0.02
-
3'-phosphoadenylylsulfate
-
pH 6.2, 37C
0.0315
-
ATEFEFLDYDFL
-, Q6WZB4
pH 5.8, 28C
0.0085
-
ATEFEYLDYDFL
-, Q6WZB4
pH 5.8, 28C
0.0052
-
ATEYEFLDYDFL
-, Q6WZB4
pH 5.8, 28C
0.0359
-
ATEYEYLDYDFL
-, Q6WZB4
pH 5.8, 28C
1.223
-
benzyl-Glu-Tyr
O60507, O60704
pH 6.0, 30C, isozyme TPST-2
1.297
-
benzyl-Glu-Tyr
O60507, O60704
pH 6.0, 30C, isozyme TPST-1
0.017
-
CCK-(107-115)
-
pH 6.5, 30C
4e-05
-
EAY
-
acidic amino acid polymer, pH 6.8, 37C
0.00012
-
EAY
-
pH acidic amino acid polymer, 6.2, 37C, presence of prostaglandin E2
0.0003
-
EAY
-
acidic amino acid polymer, pH 6.6
0.00041
-
EAY
-
acidic amino acid polymer, pH 6.8, 37C, rat liver, control group without chronic alcohol feeding
0.00043
-
EAY
-
acidic amino acid polymer, pH 6.8, 37C, rat liver after chronic alcohol feeding
0.0014
-
EAY
-
acidic amino acid polymer, pH 6.2, 37C, absence of prostaglandin E2
0.003
-
EAY
-
acidic amino acid polymer, pH 6.2, 37C
0.071
-
EEFHTDYIYTQDVK
-
PP-PSK-(74-87), truncated sequence of preprophytosulfokinase, pH 7, 30C
0.457
-
EEFHTDYIYTQDVK
-
PP-PSK-(74-87), truncated sequence of preprophytosulfokinase, pH 7, 30C
0.0017
-
KKE(EPEYGE)2
-
pH 6.5, 30C
4.4e-05
-
KKE(EPEYGE)3
-
pH 6.5, 30C
-
0.043
-
KKEEPEYGE
-
pH 6.5, 30C
0.00034
-
KKEEPEYGE(EPEFGE)2
-
pH 6.5, 30C
0.021
-
KKSAEDYEYPSMG
-
pH 6.5, 30C
0.37
-
KKSAEDYQYPSMG
-
pH 6.5, 30C
0.55
-
KKSAENYEYPSMG
-
pH 6.5, 30C
0.011
-
P-selectin glycoprotein ligand-1
-
using glutathione S-transferase-tagged P-selectin glycoprotein ligand-1 as cosubstrate, in 50 mM MES buffer (pH 6.5), 5 mM 2-mercaptoethanol, at 37C
-
0.053
-
P-selectin glycoprotein ligand-1
-
using P-selectin glycoprotein ligand-1 as cosubstrate, in 50 mM MES buffer (pH 6.5), 5 mM 2-mercaptoethanol, at 37C
-
0.11
-
peptide CCK-(107-115)
-
pH 6.5, 30C
0.15
-
peptide CCK-2
-
pH 6.5, 30C
0.12
-
peptide CCK-3
-
pH 6.5, 30C
0.035
-
peptide CCKI
-
pH 6.5, 30C
-
0.0015
0.0016
peptide EAY
-
-
-
0.0015
0.0016
peptide EAY
-
-
-
0.24
-
peptide Hir-(57-65)
-
pH 6.5, 30C
0.41
-
peptide Hir-(57-65)
-
pH 6.5, 30C
0.14
-
peptide tub-1
-
pH 6.5, 30C
0.0097
-
PSGL-1
O60507, O60704
pH 6.0, 30C, isozyme TPST-1
-
0.0269
-
PSGL-1
O60507, O60704
pH 6.0, 30C, isozyme TPST-2
-
5.6
-
recombinant hirudin HV2
-
pH 6.5, 30C
-
0.04
-
statherin
-
pH 6.5, 37C
-
0.04
-
statherin
-
-
-
0.0175
-
Tyr-Tyr-Tyr
O60507, O60704
pH 6.0, 30C, isozyme TPST-1
0.022
-
Tyr-Tyr-Tyr
O60507, O60704
pH 6.0, 30C, isozyme TPST-2
2
3
VTDsYYsYPDI
-
disulfation of sY15CCR8
17
-
VTDsYYsYPDI
-
disulfation of sY15CCR8, mixture of TPST-1 and TPST-2 1:1; disulfation of sY15CCR8, mixture of TPST-1 and TPST-2 1:1
21
-
VTDsYYsYPDI
-
disulfation of sY15CCR8
0.0031
-
VTDsYYYPDI
-
in 20 mM MOPS, at pH 7.5, 100 mM NaCl, at 30C
-
0.0193
-
VTDYYYPDI
-
in 20 mM MOPS, at pH 7.5, 100 mM NaCl, at 30C
75
-
VTDYYYPDI
-
monosulfation of non CCR8, mixture of TPST-1 and TPST-2 1:1; monosulfation of non CCR8, mixture of TPST-1 and TPST-2 1:1
99
-
VTDYYYPDI
-
monosulfation of non CCR8
120
-
VTDYYYPDI
-
monosulfation of non CCR8
0.59
-
KKSAENYQYPSMG
-
pH 6.5, 30C
additional information
-
additional information
-
kinetic properties with synthetic peptides as substrates, with modified lengths on NH2- or COOH-termini, changes in Km-values resulting from substitution of negatively charged amino acids in the region of the acceptor tyrosine
-
additional information
-
additional information
-
kinetic properties with synthetic peptides as substrates
-
additional information
-
additional information
-
kinetic properties with synthetic peptides as substrates; the Km-values of peptides with multiple Tyr-sulfation sites decrease exponentially with the number of sites
-
additional information
-
additional information
-
-
-
additional information
-
additional information
O60507, O60704
;
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.058
-
3'-phosphoadenylyl sulfate
-
using glutathione S-transferase-tagged P-selectin glycoprotein ligand-1 as cosubstrate, in 50 mM MES buffer (pH 6.5), 5 mM 2-mercaptoethanol, at 37C
0.11
-
3'-phosphoadenylyl sulfate
-
using P-selectin glycoprotein ligand-1 as cosubstrate, in 50 mM MES buffer (pH 6.5), 5 mM 2-mercaptoethanol, at 37C
0.045
-
P-selectin glycoprotein ligand-1
-
using glutathione S-transferase-tagged P-selectin glycoprotein ligand-1 as cosubstrate, in 50 mM MES buffer (pH 6.5), 5 mM 2-mercaptoethanol, at 37C
-
0.1
-
P-selectin glycoprotein ligand-1
-
using P-selectin glycoprotein ligand-1 as cosubstrate, in 50 mM MES buffer (pH 6.5), 5 mM 2-mercaptoethanol, at 37C
-
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
6.7
-
3'-phosphoadenylyl sulfate
-
using P-selectin glycoprotein ligand-1 as cosubstrate, in 50 mM MES buffer (pH 6.5), 5 mM 2-mercaptoethanol, at 37C
228142
18.2
-
3'-phosphoadenylyl sulfate
-
using glutathione S-transferase-tagged P-selectin glycoprotein ligand-1 as cosubstrate, in 50 mM MES buffer (pH 6.5), 5 mM 2-mercaptoethanol, at 37C
228142
2
-
P-selectin glycoprotein ligand-1
-
using P-selectin glycoprotein ligand-1 as cosubstrate, in 50 mM MES buffer (pH 6.5), 5 mM 2-mercaptoethanol, at 37C
0
4.2
-
P-selectin glycoprotein ligand-1
-
using glutathione S-transferase-tagged P-selectin glycoprotein ligand-1 as cosubstrate, in 50 mM MES buffer (pH 6.5), 5 mM 2-mercaptoethanol, at 37C
0
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.031
-
3'-phosphoadenylyl sulfate
-
using glutathione S-transferase-tagged P-selectin glycoprotein ligand-1 as substrate,in 50 mM MES buffer (pH 6.5), 5 mM 2-mercaptoethanol, at 37C
0.061
-
3'-phosphoadenylyl sulfate
-
using P-selectin glycoprotein ligand-1 as substrate,in 50 mM MES buffer (pH 6.5), 5 mM 2-mercaptoethanol, at 37C
0.00016
-
adenosine 3',5'-bisphosphate
-
with VTDsYYYPDI as substrate, in 20 mM MOPS, at pH 7.5, 100 mM NaCl, at 30C
0.00074
-
adenosine 3',5'-bisphosphate
-
with VTDYYYPDI as substrate, in 20 mM MOPS, at pH 7.5, 100 mM NaCl, at 30C
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
3.1e-06
-
O60507, O60704
recombinant TPST-1 in HEK-293T cell extract
3.2e-06
-
O60507, O60704
recombinant TPST-2 in HEK-293T cell extract
0.0057
-
-
-
6.3
-
-
-
additional information
-
-
development of a specific detection method using an anti-sulfotyrosine monoclonal antibody, termed PSG2, that binds with high affinity and exquisite specificity to sulfotyrosine residues in peptides and proteins independently of sequence context, overview
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.8
-
-, Q6WZB4
-
6
6.5
-
Golgi-enzyme, sulfation of tert-butoxycarbonylcholecystokinin
6
-
-
PKG
6
-
O60507, O60704
TPST-2
6
-
-
isozyme TPST-2
6.3
-
-
-
6.4
6.6
-
solubilized enzyme
6.4
-
-
-
6.5
-
O60507, O60704
TPST-1
6.5
-
-
-
6.5
-
-
optimal sulfatation of statherin at pH 6.5 and at 20 mM MnCl2
6.5
-
-
isozyme TPST-1
6.7
-
-
liver; sulfation of acidic amino acid polymer EAY
6.7
-
-
sulfation of acidic amino acid polymer EAY
6.8
-
-
-
7.2
-
-
assay at
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.5
6.5
-, Q6WZB4
pH 5.5: about 95% of maximal activity, pH 6.5: about 55% of maximal activity
5.7
6.8
-
about half-maximal activity at pH 5.7 and 6.8
6.2
7.2
-
sulfation of acidic amino acid polymer EAY, liver, about half-maximal activity at pH 6.2 and 7.2
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
22
-
-
assay at room temperature
30
-
-
assay at
30
-
-
assay at
30
-
-
assay at
30
-
O60507, O60704
assay at; assay at
37
-
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
TPST-2 shows higher expression as TPST-1
Manually annotated by BRENDA team
O60507, O60704
adult and fetal; adult and fetal
Manually annotated by BRENDA team
-
primary culture of umbilical vein endothelial cells, 2 isoforms: TPST1 and TPST2, shear stress causes a shift in protein expression from TPST1 to TPST2
Manually annotated by BRENDA team
-
mainly expressed in hypodermis, especially in the seam cells
Manually annotated by BRENDA team
O60507
peripheral leukocyte; peripheral leukocyte
Manually annotated by BRENDA team
-
high TPST-2 expression level
Manually annotated by BRENDA team
-
low TPST-2 expression level
Manually annotated by BRENDA team
O60507, O60704
;
Manually annotated by BRENDA team
O60507
; highest expression of TPST-2 mRNA
Manually annotated by BRENDA team
-
fast-responding clone
Manually annotated by BRENDA team
-
isozymes TPST-1 and TPST-2
Manually annotated by BRENDA team
-
from healthy individuals
Manually annotated by BRENDA team
-
antrum and body mucosa
Manually annotated by BRENDA team
O60507, O60704
;
Manually annotated by BRENDA team
O60507
; highest expression of TPST-1 mRNA
Manually annotated by BRENDA team
-
TPST-1 is expressed at substantially higher levels than TPST-2
Manually annotated by BRENDA team
-
TPST-2 shows higher expression as TPST-1
Manually annotated by BRENDA team
-
TPST-2 shows higher expression as TPST-1
Manually annotated by BRENDA team
-
breast cancer cell line, isozyme TPST-2
Manually annotated by BRENDA team
additional information
-
tissue distribution
Manually annotated by BRENDA team
additional information
O60507, O60704
broad tissue distribution of isozyme TPST-1, overview; broad tissue distribution of isozyme TPST-2, overview
Manually annotated by BRENDA team
additional information
-
isozyme genes Tpst1 and Tpst2 tissue expression analysis
Manually annotated by BRENDA team
additional information
O60507
mRNA expression profile of the sulfotransferase in 23 different human tissues; mRNA expression profile of the sulfotransferase in 23 different human tissues
Manually annotated by BRENDA team
additional information
-
the expression of TPST2 protein in various tissues of wild-type DW/J mice
Manually annotated by BRENDA team
additional information
-
wide distributed tissue expression pattern of isozymes TPST-1 and TPST-2, in several tissues, TPST-2 shows higher expression as TPST-1, expression pattern, overview
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
O60507, O60704
TPST-1 displays type II transmembrane topology with a short N-terminal cytoplasmic domain, a single 17-residue transmembrane domain, and a luminal catalytic domain; TPST-2 displays type II transmembrane topology with a short N-terminal cytoplasmic domain, a single 17-residue transmembrane domain, and a luminal catalytic domain
Manually annotated by BRENDA team
-
the transmembrane domain of TPST1 is determined to act in targeting and retention of the enzyme to the trans-Golgi compartment
Manually annotated by BRENDA team
-
trans-Golgi network; trans-Golgi network
Manually annotated by BRENDA team
-
a type-II membrane protein that exposes the catalytic domain in Golgi lumen
Manually annotated by BRENDA team
-
the enzyme is localized to the trans-Golgi network
Manually annotated by BRENDA team
Caenorhabditis elegans N2
-
the enzyme is localized to the trans-Golgi network
-
Manually annotated by BRENDA team
-
trans-most subcompartment
Manually annotated by BRENDA team
-
type II transmembrane isozymes
Manually annotated by BRENDA team
-
integral membrane-protein
Manually annotated by BRENDA team
-
integral membrane-protein
Manually annotated by BRENDA team
-, Q6WZB4
type II transmembrane protein
Manually annotated by BRENDA team
O60507, O60704
TPST-1 displays type II transmembrane topology with a short N-terminal cytoplasmic domain, a single 17-residue transmembrane domain, and a luminal catalytic domain; TPST-2 displays type II transmembrane topology with a short N-terminal cytoplasmic domain, a single 17-residue transmembrane domain, and a luminal catalytic domain
Manually annotated by BRENDA team
-
TPST1 is a transmembrane enzyme; TPST2 are transmembrane enzymes
Manually annotated by BRENDA team
-
transmembrane protein
Manually annotated by BRENDA team
-
in the trans-Golgi network the TPST is anchored to the membrane with its catalytic domain luminally oriented; in the trans-Golgi network the TPST is anchored to the membrane with its catalytic domain luminally oriented
Manually annotated by BRENDA team
additional information
-
subcellular distribution
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
42130
-
O70281
calculated from nucleotide sequence
42190
-
-
calculated from nucleotide sequence
50000
54000
-
SDS-PAGE
50000
54000
-
3 bands on SDS-PAGE
54000
-
-
SDS-PAGE
100000
-
-
enzyme-detergent micelle, glycerol gradient centrifugation
143000
-
-
-
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 50000-54000, SDS-PAGE
?
-
x * 54000, SDS-PAGE
?
-
x * 47000, SDS-PAGE
?
Caenorhabditis elegans N2
-
x * 47000, SDS-PAGE
-
additional information
O60507, O60704
TPST-1 displays type II transmembrane topology with a short N-terminal cytoplasmic domain, a single 17-residue transmembrane domain, and a luminal catalytic domain; TST-2 displays type II transmembrane topology with a short N-terminal cytoplasmic domain, a single 17-residue transmembrane domain, and a luminal catalytic domain
additional information
-
epitope mapping
additional information
-
TSPT-1 and TPST-2 are predicted to have type II transmembrane topology with a short 8-residue N-terminal cytoplasmic domain, an about 17-residue transmembrane domain and a luminal catalytic domain
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
glycoprotein
-
-
glycoprotein
-
-
glycoprotein
-
N-glycosylated
glycoprotein
O60507, O60704
TPST-1 has two putative N-glycosylation sites; TPST-2 has two putative N-glycosylation sites
glycoprotein
-
TPST1 bears N-linked glycosyl residues exclusively at position Asn60 and Asn262
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
determination of crystal and three dimensional structures of the isozymes
-
in complex with substrate peptide C4P5Y3
-
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
half-life of 30 h in vivo
-
stable in cell lysates prepared by freeze-thawing in 100 mM HEPES-NaOH buffer, pH 7.4, 20 mM MgCl2, 10 mM mercaptoethanol or in 5 mM EDTA or 0.32 M sucrose or by hypoosmotic lysis using 5 mM EDTA, no activity in cell lysates prepared in the presence of Triton X-100 or by sonication
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
4C, detergent-solubilized enzyme preparation, t1/2: 48 h
-
-40C, in 10 mM Tris-HCl, pH 7.4, 25% glycerol, 5 mM mercaptoethanol, 1% Triton X-100, 3 days
-
-80C, in 10 mM HEPES-buffer, pH 7, 50 mM NaCl, 1% Triton X-100, 1 mM DTT, 25% glycerol, several months
-
-80C, in 25% glycerol, 30% loss of activity within 1 month
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
partial
-
treatment of membrane-bound enzyme with carbonate, followed by solubilization and affinity chromatography on a substrate peptide
-
Ni-NTA agarose bead chromatography, and gel filtration
-
2 recombinant isoforms: TPST1 and TPST2
-
anti-protein C immunoaffinity chromatography; anti-protein C immunoaffinity chromatography
-
HPC4 affinity column chromatography, and gel filtration
-
partial
-
recombinant enzyme
-
develoment of an immunoaffinity purification method using anti-sulfotyrosine monoclonal antibody PSG2, recombinantly expressed in CHO cells
-
homogeneity
-
liver; partial
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
TPST-1, sequence comparison
-
expressed in HEK-293 cells
-
expression in COS-7 cells
-, Q6WZB4
substance protein A-fused tyrosylprotein sulfotransferase is expressed in Escherichia coli BL21(DE3) cells
-
TPST DNA and amino acid sequence determinationand analysis, sequence comparison
-
2 isoforms: TPST1 and TPST2
-
expressed in CHO cells
-
expressed in CHO-K1 cells
-
expressed in Escherichia coli BL21(DE3) cells
-
expression in HEK-293T cells; expression in HEK-293T cells
-
expression of isozyme TPST-1 in HEK-293T cells, quantitative real-time PCR expression analysis; expression of isozyme TPST-2 in HEK-293T cells, quantitative real-time PCR expression analysis
O60507, O60704
expression of TPSt-1 in HeLa cells and COS-7 cells as GFP-tagged protein in the Golgi compartment
-
isozymes TPST-1 and TPST-2, DNA and amino acid sequence determinationand analysis, sequence comparison
-
-
O70281
gene Tpst2, wild-type and mutant enzymes genotyping and genetic mapping, expression of genes Tpst1 and Tpst2 in grt mouse cells, rescue of the phenotype, overview
-
isozyme TPST-2, expression analysis
-
isozymes TPST-1 and TPSt-2, geno- and phenotyping of wild-types and mutants
-
isozymes TPST-1 and TPST-2, genotyping
-
isozymes TPST-1 and TPST-2. TPST-1 DNA and amino acid sequence determinationand analysis, sequence comparison
-
TPST-1, sequence comparison
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
N262A
-
site-directed mutagenesis, the N-glycosylation site mutant is less N-glycosylated and has a lower molecular weight compared to the wild-type enzyme
N60A
-
site-directed mutagenesis, the N-glycosylation site mutant is less N-glycosylated and has a lower molecular weight compared to the wild-type enzyme
additional information
-
consruction of truncation mutants lacking the cytosolic or the transmembrane domain, the first are not affected in their subcellular localization in the Golgi compartment, the latter are mislocated in the cytosol, overview
additional information
-
conjugation of protein, e.g. the receptor-binding nontoxic B-subunit of Shiga toxin, with N-carbamoyl-succinate-modified peptides, method overview
H266Q
-
missense mutation of Tpst2, devoid of activity, in the highly conserved region of the gene leads to the autosomal recessive, fetal-onset, severe thyroid hypoplasia phenotype related to TSH hyporesponsiveness in growth-retarded mice, the mutant enzyme shows unaltered subcellular localization, overview
additional information
-
generation of Tpst1 and Tpst2 double knock-out mice; generation of Tpst1 and Tpst2 double knock-out mice, certain sperm/epididymal proteins are undersulfated in Tpst2-/- mice
additional information
-
targeted disruption of TPST-2, but not of TPST-1, causes male infertility, the reproductive performance of femal and male mice is reduced in TPST-2-/- mice, Tpst-1-/- males that have normal fertility, while Tpst-2-/- males are infertile, adhesion of Tpst2-/- sperm to the egg plasma membrane is reduced compared with wild type sperm, phenotypes, overview
additional information
-
isozymes TPST-1 and TPSt-2, generation of single and double knockout mice. Double knockout mice completely lack the ability to sulfate peptidyltyrosine, Tpst2-/- mice show primary hypothyroidism, but that Tpst1-/- mice are euthyroid, phenotypes, overview
additional information
-
generation of isozyme TPST-2 deficient growth-retarded mutant mice, phenotype, overview
additional information
-
generation of homozygous and heterozygous TPST-1 and/or TPST-2 knockout mutant mice. Disruption of one allele at the TPST-1 locus has no significant effect, and TPST-1+/- mice appear normal, have unaltered fertility and growth curves. TPST-1 -/- mice show reduced postnatal body weight. Disruption of the TPST-2 gene also causes no significant effect in TPST-2+/- mice, but TPST-2-/- mice also show reduced postnatal body weight like TPST-1 -/-mice, but reached normal body weight at 10 weeks of age. The most significant difference between TPST-1 -/- and TPST-2 -/- is the reduced fertility of TPST-2 knockout mice
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
molecular biology
-
conjugation of proteins with N-carbamoyl-succinate-modified peptides is an appropriate tool in research, for instance, in the development of vaccines and drugs or for studying biological mechanisms