Information on EC 2.6.1.19 - 4-aminobutyrate-2-oxoglutarate transaminase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.6.1.19
-
RECOMMENDED NAME
GeneOntology No.
4-aminobutyrate-2-oxoglutarate transaminase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
amino group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
4-aminobutanoate degradation I
-
-
4-aminobutanoate degradation II
-
-
4-aminobutanoate degradation III
-
-
4-aminobutanoate degradation V
-
-
alanine metabolism
-
-
Alanine, aspartate and glutamate metabolism
-
-
beta-alanine degradation I
-
-
beta-Alanine metabolism
-
-
Butanoate metabolism
-
-
GABA shunt
-
-
glutamate and glutamine metabolism
-
-
L-glutamate degradation IV
-
-
Metabolic pathways
-
-
nicotine degradation I (pyridine pathway)
-
-
Propanoate metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
4-aminobutanoate:2-oxoglutarate aminotransferase
Requires pyridoxal phosphate. Some preparations also act on beta-alanine, 5-aminopentanoate and (R,S)-3-amino-2-methylpropanoate.
CAS REGISTRY NUMBER
COMMENTARY hide
9037-67-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
Candida guilliermondii var. membranaefaciens
Y43
-
-
Manually annotated by BRENDA team
Candida guilliermondii var. membranaefaciens Y43
Y43
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
streptomycin-bleached mutant strain
-
-
Manually annotated by BRENDA team
streptomycin-bleached mutant strain
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
Mongolian gerbil
-
-
Manually annotated by BRENDA team
wild-type strain 3a6A
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
F-126
-
-
Manually annotated by BRENDA team
F-126
-
-
Manually annotated by BRENDA team
isoform Uga1
UniProt
Manually annotated by BRENDA team
locust
-
-
Manually annotated by BRENDA team
isoform Uga1
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
GABA metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(1R,4S)-4-amino-2-cyclopentene-1-carboxylic acid + 2-oxoglutarate
? + L-glutamate
show the reaction diagram
-
analogue of 4-aminobutanoate, vigabatrin
-
-
?
(4R)-4-amino-1-cyclopentene-1-carboxylic acid + 2-oxoglutarate
4-oxo-1-cyclopentene1-carboxylic acid + L-glutamate
show the reaction diagram
-
analogue of 4-aminobutanoate, vigabatrin
-
-
?
(R)-4-amino-3-fluorobutanoic acid
4-aminobut-2-enoic acid + HF
show the reaction diagram
-
-
-
-
?
(R,S)-4-amino-3-fluorobutanoic acid
4-aminobut-2-enoic acid + HF
show the reaction diagram
-
neither enantiomer is a substrate for transamination. The rate of elimination of HF from the (R)-enantiomer is at least 10 times greater than that for the (S)-enantiomer
-
-
?
(S)-4-amino-4,5-dihydro-2-thiophenecarboxylic acid + 2-oxoglutarate
4-oxo-4,5-dihydro-2-thiophenecarboxylic acid + L-glutamate
show the reaction diagram
-
mechanism-based inactivator that partly undergoes inactivation
-
-
?
1H-tetrazole-5-butanamine + 2-oxoglutarate
(1H-tetrazol-5-yl)-butyraldehyde + L-glutamate
show the reaction diagram
-
-
-
-
?
1H-tetrazole-5-ethanamine + 2-oxoglutarate
(1H-tetrazol-5-yl)-acetaldehyd + L-glutamate
show the reaction diagram
-
-
-
-
?
1H-tetrazole-5-propanamine + 2-oxoglutarate
(1H-tetrazol-5-yl)-propionaldehyde + L-glutamate
show the reaction diagram
-
-
-
-
?
3-(aminomethyl)benzoic acid + 2-oxoglutarate
3-(iminomethyl)benzoic acid + L-glutamate
show the reaction diagram
-
3.4% of the activity with 4-aminobutanoate
-
-
?
3-aminoisobutanoate + 2-oxoglutarate
L-glutamate + 3-oxoisobutanoate
show the reaction diagram
3-aminopropanesulfonate + 2-oxoglutarate
3-sulfopropanal + L-glutamate
show the reaction diagram
substrate is homotaurine
-
-
?
4-(aminomethyl)-1H-pyrrole-2-carboxylic acid + 2-oxoglutarate
?
show the reaction diagram
-
-
-
-
?
4-(aminomethyl)furan-2-carboxylic acid + 2-oxoglutarate
?
show the reaction diagram
-
-
-
-
?
4-(aminomethyl)furan-3-carboxylic acid + 2-oxoglutarate
?
show the reaction diagram
-
-
-
-
?
4-(aminomethyl)thiophene-2-carboxylic acid + 2-oxoglutarate
?
show the reaction diagram
-
-
-
-
?
4-(aminomethyl)thiophene-3-carboxylic acid + 2-oxoglutarate
?
show the reaction diagram
-
-
-
-
?
4-aminobutanoate + 2-oxoglutarate
4-oxobutanoate + L-glutamate
show the reaction diagram
4-aminobutanoate + glyoxylate
4-oxobutanoate + glycine
show the reaction diagram
4-aminobutanoate + pyruvate
4-oxobutanoate + alanine
show the reaction diagram
4-aminobutanoate + pyruvate
4-oxobutanoate + L-alanine
show the reaction diagram
4-aminobutyrate + 2-oxoglutarate
4-oxobutanoate + L-glutamate
show the reaction diagram
-
-
-
?
5-(aminomethyl)-1H-pyrrole-2-carboxylic acid + 2-oxoglutarate
?
show the reaction diagram
-
-
-
-
?
5-(aminomethyl)furan-2-carboxylic acid + 2-oxoglutarate
?
show the reaction diagram
-
-
-
-
?
5-(aminomethyl)thiophene-2-carboxylic acid + 2-oxoglutarate
?
show the reaction diagram
-
-
-
-
?
5-aminopentanoate + 2-oxoglutarate
L-glutamate + 5-oxopentanoate
show the reaction diagram
6-aminohexanoate + 2-oxoglutarate
L-glutamate + 6-oxohexanoate
show the reaction diagram
beta-alanine + 2-oxoglutarate
malonic semialdehyde + L-glutamate
show the reaction diagram
cadaverine + 2-oxoglutarate
L-glutamate + 5-aminopentanal
show the reaction diagram
-
-
-
-
?
cadaverine + 2-oxoglutarate
L-glutamate + ?
show the reaction diagram
-
poor amino donor
-
-
r
D-lysine + 2-oxoglutarate
L-glutamate + 5-aminopentanol
show the reaction diagram
DL-3-amino-1-cyclopentene-1-carboxylic acid + 2-oxoglutarate
3-oxo-1-cyclopentene-1-carboxylic acid + L-glutamate
show the reaction diagram
-
analogue of 4-aminobutanoate, vigabatrin
-
-
?
DL-3-hydroxy-4-aminobutanoate + 2-oxoglutarate
L-glutamate + 3-hydroxy-4-oxobutanoate
show the reaction diagram
DL-ornithine + 2-oxoglutarate
L-glutamate + 4-methyl-2-oxopentanoate
show the reaction diagram
gamma-aminobutyric acid
?
show the reaction diagram
-
-
-
-
?
hypotaurine + 2-oxoglutarate
L-glutamate + 2-oxoethanesulfinic acid
show the reaction diagram
-
poor amino donor
-
-
r
L-lysine + 2-oxoglutarate
L-glutamate + (S)-2-amino-6-oxohexanoate
show the reaction diagram
-
-
i.e. L-2-aminoadipate-6-semialdehyde
-
?
L-ornithine + 2-oxoglutarate
L-glutamate + (S)-2-amino-5-oxopentanoate
show the reaction diagram
-
-
i.e. L-glutamate-gamma-semialdehyde
-
?
putrescine + 2-oxoglutarate
L-glutamate + 4-aminobutanal
show the reaction diagram
-
-
-
-
?
putrescine + 2-oxoglutarate
L-glutamate + 4-aminobutanol
show the reaction diagram
-
poor amino donor
-
-
r
tetrazole-5-butanamine + 2-oxoglutarate
?
show the reaction diagram
-
-
-
-
?
tetrazole-5-ethanamine + 2-oxoglutarate
?
show the reaction diagram
-
-
-
-
?
tetrazole-5-propanamine + 2-oxoglutarate
?
show the reaction diagram
-
-
-
-
?
[2-(aminomethyl)phenyl]acetic acid + 2-oxoglutarate
(2-formylphenyl)acetic acid + L-glutamate
show the reaction diagram
-
5.65% of the activity with 4-aminobutanoate
-
-
?
[3-(aminomethyl)phenyl]acetic acid + 2-oxoglutarate
(3-formylphenyl)acetic acid + L-glutamate
show the reaction diagram
-
0.78% of the activity with 4-aminobutanoate
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-aminopropanesulfonate + 2-oxoglutarate
3-sulfopropanal + L-glutamate
show the reaction diagram
Q0K2K2
substrate is homotaurine
-
-
?
4-aminobutanoate + 2-oxoglutarate
4-oxobutanoate + L-glutamate
show the reaction diagram
4-aminobutyrate + 2-oxoglutarate
4-oxobutanoate + L-glutamate
show the reaction diagram
Q0K2K2
-
-
-
?
additional information
?
-
-
enzyme is induced in cells grown on 4-guanidinobutyrate, 4-aminobutyrate, or putrescine as the only carbon and nitrogen source. GABAT functions in the biosynthesis of arginine by converting N2-acetyl-L-glutamate 5-semialdehyde to N2-acetyl-L-ornithine as well as in catabolic reactions during growth on putrescine or 4-guanidinobutyrate but not during growth on arginine
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
no iron-sulfur cluster
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(+/-)-(1S,2R,4S,5S)-4-amino-6,6-difluorobicyclo[3.1.0]hexane-2-carboxylic acid
-
10 mM, weak, reversible inhibitor
(+/-)-(1S,2S,4S,5S)-4-amino-6,6-difluorobicyclo[3.1.0]hexane-2-carboxylic acid
-
10 mM, weak, reversible inhibitor
(+/-)piperidine-3-sulfonic acid
-
-
(1R,3S,4S)-3-amino-4-fluorocyclopentane-1-carboxylic acid
-
mechanism-based inactivation, adduct formed is derived from enamine mechanism
(1R,4S)-4-amino-2-cyclopentene-1-carboxylic acid
-
analogue of 4-aminobutanoate, vigabatrin
(1R,4S)-4-amino-3-fluorocyclopent-2-enecarboxylic acid
-
weak reversible inhibition in the presence of beta-mercaptoethanol
(1R,4S)-4-amino-3-pentafluoroethylcyclopent-2-enecarboxylic acid
-
weak reversible inhibition in the presence of beta-mercaptoethanol
(1R,4S)-4-amino-3-trifluoromethylcyclopent-2-enecarboxylic acid
-
irreversible inhibition in the presence of beta-mercaptoethanol
(1S,3S)-(Z)-3-amino-4-(2,2,2-trifluoroethylidene)cyclopentanecarboxylic acid
-
inhibition in the presence of beta-mercaptoethanol
(1S,3S)-3-amino-4-(2,2,2-trifluoro-1-trifluoromethylethylidene)-cyclopentanecarboxylic acid
-
weak reversible inhibition in the presence of beta-mercaptoethanol
(1S,3S)-3-amino-4-difluoromethylenecyclopentanecarboxylic acid
-
; potent irreversible inhibitor
(1S,4R)-4-amino-2-cyclopentene-1-carboxylic acid
-
analogue of 4-aminobutanoate, vigabatrin
(1S,4S)-2-(difluoromethylidene)-4-(1H-tetrazol-5-yl)cyclopentanamine
-
time-dependent inactivation, ratio kinact/KI value at pH 8.0 is 2.48 per min and mM
(2E)-4-methylpentan-2-one N-(2,4-dimethylphenyl)semicarbazone
-
57% inhibition at 0.125 mM
(2E)-butan-2-one N-(2,4-dimethylphenyl)semicarbazone
-
89% inhibition at 0.0625 mM
(4R)-4-amino-1-cyclopentene-1-carboxylic acid
-
analogue of 4-aminobutanoate, vigabatrin
(4S)-4-amino-1-cyclopentene-1-carboxylic acid
-
analogue of 4-aminobutanoate, vigabatrin
(R,S)-4-amino-3-fluorobutanoic acid
-
the (R)-enantiomer inhibits the transamination of gamma-aminobutanoic acid 10 times more effectively than the (S)-enantiomer. On binding of free 4-amino-3-fluorobutanoic acid to enzyme the optimal conformation places the C-NH3 + and C-F bonds gauche in the (R)-enantiomer but anti in the (S)-enantiomer
(S)-4-amino-4,5-dihydro-2-thiophenecarboxylic acid
-
mechanism-based inactivator, reacts via aromatization mechanism
(Z)-4-amino-2-butenoic acid
-
-
1H-tetrazole-5-(alpha-vinyl-propanamine)
-
-
2,4-diaminobutanoate
2,4-dimethylphenyl semicarbazide hydrochloride
-
90% inhibition at 0.0625 mM
2-Aminobenzenesulfonate
-
-
2-aminobutanoate
Candida guilliermondii var. membranaefaciens
-
-
2-aminoethane phosphonic acid
-
-
2-N-(acetylamino)cyclohexane sulfonic acid
-
-
2-oxoadipic acid
-
-
2-oxoglutarate
2-Thiouracil
-
weak
3-(aminomethyl)benzoic acid
-
poor competitive inhibitor
3-aminocyclohexanecarboxylic acid
-
10 mM
3-chloro-1-(4-hydroxyphenyl)propan-1-one
-
irreversible and potent inhibitor, about 30% residual activity at 0.06 mM, 2-oxoglutarate prevents the enzyme from inactivation
3-Chloro-4-aminobutanoate
-
-
3-Mercaptopropionic acid
-
-
3-Methyl-2-benzothiazolone hydrazone hydrochloride
-
-
3-Phenyl-4-aminobutanoate
-
-
4-(aminomethyl)-1H-pyrrole-2-carboxylic acid
-
-
4-(aminomethyl)furan-2-carboxylic acid
-
-
4-(aminomethyl)furan-3-carboxylic acid
-
-
4-(aminomethyl)thiophene-2-carboxylic acid
-
-
4-(aminomethyl)thiophene-3-carboxylic acid
-
-
4-acryloylphenol
-
potent inhibitor
4-amino-2-fluorobutanoate
-
reversible, competitive to 4-aminobutanoate
4-amino-5-fluoropentanoic acid
-
potent irreversible inhibitor
4-Amino-hex-5-enoic acid
-
substrate analogue, irreversible, in vitro and in vivo
4-aminohex-5-enoic acid
4-Aminohex-5-ynoic acid
-
irreversible, in vitro and in vivo, kinetics
4-ethynyl-4-aminobutanoate
-
-
4-hydroxybenzaldehyde
4-hydroxybenzylamine
-
IC50: 0.0154 mM, competitive inhibition
5,5'-dithiobis-2-nitrobenzoic acid
-
95% loss of activity
5-(aminomethyl)-1H-pyrrole-2-carboxylic acid
-
-
5-(aminomethyl)furan-2-carboxylic acid
-
-
5-(aminomethyl)thiophene-2-carboxylic acid
-
-
5-amino-1,3-cyclohexadienylcarboxylate
;
5-Diazouracil
-
weak
5-fluorouracil
5-Iodouracil
-
84% inhibition at 1 mM
5-Nitrouracil
-
weak
5-Thiouracil
-
weak
6-Azathymine
-
-
6-Azauracil
-
63% inhibition at 1 mM, reversible by dialysis, not by pyridoxal phosphate addition
acetic acid
-
-
adipic acid
-
-
ADP
-
reversible
alpha-alanine
Candida guilliermondii var. membranaefaciens
-
-
Aminooxyacetate
ATP
-
reversible
Ba2+
-
order of decreasing inhibitory potency: Hg2+, Cd2+, Cu2+, Co2+, Ba2+, Sr2+, Ni2+, Mn2+, Ca2+, Mg2+
Baclofen
-
injection of 0.01 mg/g body weight reduces GABA-T mRNA level 2fold; i.p. injection of sexually regressed female goldfish results in significant increase in serum luteinising hormone after 6 h. About 2fold decrease both in glutamic acid decarboxylase 67 and gamma-aminobutanoate transaminase mRNa in the hypothalamus
beta-Alanine
Branched-chain fatty acids
Butyric acid
Ca2+
-
order of decreasing inhibitory potency: Hg2+, Cd2+, Cu2+, Co2+, Ba2+, Sr2+, Ni2+, Mn2+, Ca2+, Mg2+
Carbonyl reagents
Cd2+
-
order of decreasing inhibitory potency: Hg2+, Cd2+, Cu2+, Co2+, Ba2+, Sr2+, Ni2+, Mn2+, Ca2+, Mg2+
cis-3-aminocyclohex-4-ene-1-carboxylic acid
-
conformationally rigid analogue of vigabatrin, mechanism
Co2+
-
order of decreasing inhibitory potency: Hg2+, Cd2+, Cu2+, Co2+, Ba2+, Sr2+, Ni2+, Mn2+, Ca2+, Mg2+
cycloserine
-
90% inhibition at 1 mM
D-cycloserine
D-penicillamine
dioxan
-
5% v/v
Divalent metal ions
-
with decreasing efficiency: Hg2+, Cd2+, Co2+, Ba2+, Sr2+, Ni2+, Mn2+, Ca2+, Mg2+
-
DL-3-amino-1-cyclopentene-1-carboxylic acid
-
analogue of 4-aminobutanoate, vigabatrin
DL-cysteine
-
-
DL-trans-4-amino-2-cyclopentene-1-carboxylic acid
-
analogue of 4-aminobutanoate, vigabatrin
ethanol
ethanolamine O-sulfate
-
active-site directed, ir, in vitro and in vivo, kinetics
ethylamine-2-sulfonic acid
-
i.e. taurine, competitive
falcarindiol
-
active-site directed, irreversible, 23% residual activity at 14 mM, isolate of root of Angelica dahurica
gabaculine
gamma-vinyl 4-aminobutanoate
-
0.1 mM, complete inhibition
gastrodigenin
-
30.87% inhibition at 0.01 mM
GDP
-
reversible
glutamic acid
-
-
Glutarate
-
-
glycine
competitive inhibitor of pyruvate-dependent GABA-T activity
glyoxylate
-
weak
hydrazine
hydroxylamine
imperatorin
-
active-site directed, irreversible, 14% residual activity at 14 mM, isolate of root of Angelica dahurica
Lysyl reagents
-
2-oxoglutarate protects
-
Maleate
-
-
methanol
-
10% v/v, weak
Mg2+
-
order of decreasing inhibitory potency: Hg2+, Cd2+, Cu2+, Co2+, Ba2+, Sr2+, Ni2+, Mn2+, Ca2+, Mg2+
Mn2+
-
order of decreasing inhibitory potency: Hg2+, Cd2+, Cu2+, Co2+, Ba2+, Sr2+, Ni2+, Mn2+, Ca2+, Mg2+
monoiodoacetate
Muscimol
Ni2+
-
order of decreasing inhibitory potency: Hg2+, Cd2+, Cu2+, Co2+, Ba2+, Sr2+, Ni2+, Mn2+, Ca2+, Mg2+
oleanolic acid
-
20.2% inhibition at 0.1 mg/ml
ornithine
oxalacetate
-
-
p-chloromercuribenzoate
phenylhydrazine
pimelic acid
-
-
propan-2-one N-(2,4-dimethylphenyl)semicarbazone
-
44% inhibition at 0.25 mM
Propionic acid
rosmarinic acid
-
40.2% inhibition at 0.1 mg/ml
S-vigabatrin
-
ratio kinact/KI is1.7 per min and mM at pH 8.5, 0.11per min and mM at pH 6.5, respectively
SH-group reagents
Sr2+
-
order of decreasing inhibitory potency: Hg2+, Cd2+, Cu2+, Co2+, Ba2+, Sr2+, Ni2+, Mn2+, Ca2+, Mg2+
succinate
-
-
Succinic semialdehyde
tetrazole-5-(alpha-vinyl-propanamine)
-
-
trimethylcitryl-beta-D-galactopyranoside
-
56.8% inhibition at 0.01 mM
ursolic acid
-
19.9% inhibition at 0.1 mg/ml
Valproic acid
-
65.4% inhibition at 0.01 mM
vigabatrin
[2-(aminomethyl)phenyl]acetic acid
-
poor competitive inhibitor
[3-(aminomethyl)phenyl]acetic acid
-
poor competitive inhibitor
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4-aminobutyrate
induction during growth with 4-aminobutyrate (GABA)
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.1 - 1.6
(4R)-4-amino-1-cyclopentene-1-carboxylic acid
0.059
(R)-4-amino-3-fluorobutanoic acid
-
pH 8.5
0.045
(R,S)-4-amino-3-fluorobutanoic acid
-
pH 8.5
8
1H-tetrazole-5-butanamine
-
pH 8.5
2.3
1H-tetrazole-5-ethanamine
-
pH 8.5
2.4
1H-tetrazole-5-propanamine
-
pH 8.5
0.0021 - 46
2-oxoglutarate
8.5
3-Aminopropanesulfonate
Km apparent value
1.4
4-(aminomethyl)-1H-pyrrole-2-carboxylic acid
-
pH 8.5, 25C
0.31
4-(aminomethyl)furan-2-carboxylic acid
-
pH 8.5, 25C
2.8
4-(aminomethyl)furan-3-carboxylic acid
-
pH 8.5, 25C
1.7
4-(aminomethyl)thiophene-2-carboxylic acid
-
pH 8.5, 25C
10
4-(aminomethyl)thiophene-3-carboxylic acid
-
pH 8.5, 25C
0.077 - 146
4-aminobutanoate
9.9
4-aminobutyrate
Km apparent value
0.014
4-Oxobutanoate
with L-alanine as cosubstrate, in 50 mM TABS (pH 9), 1.5 mM dithiothreitol, 0.625 mM EDTA, 0.1 mM pyridoxal 5'-phosphate, 10% (v/v) glycerol, at 30C
9.5
5-(aminomethyl)-1H-pyrrole-2-carboxylic acid
-
pH 8.5, 25C
2.49
5-(aminomethyl)furan-2-carboxylic acid
-
pH 8.5, 25C
6.3
5-(aminomethyl)thiophene-2-carboxylic acid
-
pH 8.5, 25C
0.1
alpha-ketoglutarate
-
pH 8.4, 25C
1.05 - 18
beta-Alanine
2.3
DL-3-amino-1-cyclopentene-1-carboxylic acid
-
-
3.2
gamma-aminobutanoate
pH 8.0, 30C
2.6
gamma-aminobutanoic acid
-
pH 8.5
0.0165 - 0.11
glyoxylate
2.72
L-3-aminoisobutanoate
-
-
2.4
L-alanine
with 4-oxobutanoate as cosubstrate, in 50 mM TABS (pH 9), 1.5 mM dithiothreitol, 0.625 mM EDTA, 0.1 mM pyridoxal 5'-phosphate, 10% (v/v) glycerol, at 30C
2.9
L-ornithine
-
-
2.5
putrescine
-
-
0.0188 - 0.24
pyruvate
8
tetrazole-5-butanamine
-
at 25C in 50 mM potassium diphosphate buffer, pH 8.5, containing 2 mM beta-mercaptoethanol
2.3
tetrazole-5-ethanamine
-
at 25C in 50 mM potassium diphosphate buffer, pH 8.5, containing 2 mM beta-mercaptoethanol
2.4
tetrazole-5-propanamine
-
at 25C in 50 mM potassium diphosphate buffer, pH 8.5, containing 2 mM beta-mercaptoethanol
additional information
additional information
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.69
1H-tetrazole-5-butanamine
Sus scrofa
-
pH 8.5
0.26
1H-tetrazole-5-ethanamine
Sus scrofa
-
pH 8.5
0.48
1H-tetrazole-5-propanamine
Sus scrofa
-
pH 8.5
0.000056
4-(aminomethyl)-1H-pyrrole-2-carboxylic acid
Sus scrofa
-
pH 8.5, 25C
0.0000383
4-(aminomethyl)furan-2-carboxylic acid
Sus scrofa
-
pH 8.5, 25C
0.0000138
4-(aminomethyl)furan-3-carboxylic acid
Sus scrofa
-
pH 8.5, 25C
0.000086
4-(aminomethyl)thiophene-2-carboxylic acid
Sus scrofa
-
pH 8.5, 25C
0.00023 - 47.4
4-aminobutanoate
10.8
4-Oxobutanoate
Arabidopsis thaliana
Q94CE5
with L-alanine as cosubstrate, in 50 mM TABS (pH 9), 1.5 mM dithiothreitol, 0.625 mM EDTA, 0.1 mM pyridoxal 5'-phosphate, 10% (v/v) glycerol, at 30C
0.000025
5-(aminomethyl)-1H-pyrrole-2-carboxylic acid
Sus scrofa
-
pH 8.5, 25C
0.00056
5-(aminomethyl)furan-2-carboxylic acid
Sus scrofa
-
pH 8.5, 25C
0.00016
5-(aminomethyl)thiophene-2-carboxylic acid
Sus scrofa
-
pH 8.5, 25C
0.74 - 9.7
glyoxylate
15.4
L-alanine
Arabidopsis thaliana
Q94CE5
with 4-oxobutanoate as cosubstrate, in 50 mM TABS (pH 9), 1.5 mM dithiothreitol, 0.625 mM EDTA, 0.1 mM pyridoxal 5'-phosphate, 10% (v/v) glycerol, at 30C
0.8 - 10.6
pyruvate
0.693
tetrazole-5-butanamine
Sus scrofa
-
at 25C in 50 mM potassium diphosphate buffer, pH 8.5, containing 2 mM beta-mercaptoethanol
0.265
tetrazole-5-ethanamine
Sus scrofa
-
at 25C in 50 mM potassium diphosphate buffer, pH 8.5, containing 2 mM beta-mercaptoethanol
0.477
tetrazole-5-propanamine
Sus scrofa
-
at 25C in 50 mM potassium diphosphate buffer, pH 8.5, containing 2 mM beta-mercaptoethanol
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
10.4 - 39
4-aminobutanoate
770
4-Oxobutanoate
Arabidopsis thaliana
Q94CE5
with L-alanine as cosubstrate, in 50 mM TABS (pH 9), 1.5 mM dithiothreitol, 0.625 mM EDTA, 0.1 mM pyridoxal 5'-phosphate, 10% (v/v) glycerol, at 30C
6454
45.7 - 88
glyoxylate
6.4
L-alanine
Arabidopsis thaliana
Q94CE5
with 4-oxobutanoate as cosubstrate, in 50 mM TABS (pH 9), 1.5 mM dithiothreitol, 0.625 mM EDTA, 0.1 mM pyridoxal 5'-phosphate, 10% (v/v) glycerol, at 30C
103
42.7 - 116
pyruvate
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.364
(+/-)piperidine-3-sulfonic acid
-
mixed inhibition at 37C
0.052
(1R,4S)-4-amino-2-cyclopentene-1-carboxylic acid
-
-
1.3
(1R,4S)-4-amino-3-fluorocyclopent-2-enecarboxylic acid
-
in potassium diphosphate buffer (pH 8.5)
2.8
(1R,4S)-4-amino-3-pentafluoroethylcyclopent-2-enecarboxylic acid
-
in potassium diphosphate buffer (pH 8.5)
4.2
(1S,3S)-3-amino-4-(2,2,2-trifluoro-1-trifluoromethylethylidene)-cyclopentanecarboxylic acid
-
in potassium diphosphate buffer (pH 8.5)
2.7
(1S,4R)-4-amino-2-cyclopentene-1-carboxylic acid
-
-
3.75
(1S,4S)-2-(difluoromethylidene)-4-(1H-tetrazol-5-yl)cyclopentanamine
-
pH 6.5
1.2
(4R)-4-amino-1-cyclopentene-1-carboxylic acid
-
-
72
(4S)-4-amino-1-cyclopentene-1-carboxylic acid
-
-
5.6
1H-tetrazole-5-(alpha-vinyl-propanamine)
-
pH 8.5
0.008 - 0.013
2,4-diaminobutanoate
0.434
2-Aminobenzenesulfonate
-
competitive inhibition at 37C
0.033
2-aminobutanoate
Candida guilliermondii var. membranaefaciens
-
-
1.01
2-aminoethane phosphonic acid
-
competitive inhibition at 37C
0.598
2-N-(acetylamino)cyclohexane sulfonic acid
-
competitive inhibition at 37C
0.00018
2-oxoglutarate
-
38C, pH 8.5
0.013
3-Mercaptopropionic acid
-
-
1.59
4-(aminomethyl)-1H-pyrrole-2-carboxylic acid
-
pH 8.5, 25C
1.64
4-(aminomethyl)furan-2-carboxylic acid
-
pH 8.5, 25C
2.27
4-(aminomethyl)furan-3-carboxylic acid
-
pH 8.5, 25C
1.35
4-(aminomethyl)thiophene-2-carboxylic acid
-
pH 8.5, 25C
2.86
4-(aminomethyl)thiophene-3-carboxylic acid
-
pH 8.5, 25C
0.47
4-acryloylphenol
-
-
0.44
4-amino-2-fluorobutanoate
-
pH 8.6, 37C
2.6 - 26
4-aminohex-5-enoic acid
2.14
5-(aminomethyl)-1H-pyrrole-2-carboxylic acid
-
pH 8.5, 25C
1.4
5-(aminomethyl)furan-2-carboxylic acid
-
pH 8.5, 25C
2.49
5-(aminomethyl)thiophene-2-carboxylic acid
-
pH 8.5, 25C
0.039
alpha-alanine
Candida guilliermondii var. membranaefaciens
-
-
0.24 - 1.13
Aminooxyacetate
3.7
ATP
-
-
0.0355 - 0.55
beta-Alanine
0.002
Butyric acid
Candida guilliermondii var. membranaefaciens
-
-
0.6
DL-3-amino-1-cyclopentene-1-carboxylic acid
-
-
38
DL-trans-4-amino-2-cyclopentene-1-carboxylic acid
-
-
0.0044
ethanolamine O-sulfate
-
37C, pH 8.4
68
ethylamine-2-sulfonic acid
-
pH 8.6, 25C
19 - 233
Glutarate
3.9 - 780
Maleate
0.003
Propionic acid
Candida guilliermondii var. membranaefaciens
-
-
27 - 720
succinate
0.0066
Succinic semialdehyde
-
38C, pH 8.5
5.6
tetrazole-5-(alpha-vinyl-propanamine)
-
-
0.32 - 2.6
vigabatrin
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00399
3-chloro-1-(4-hydroxyphenyl)propan-1-one
Homo sapiens
-
-
0.0165
4-hydroxybenzaldehyde
0.0154
4-hydroxybenzylamine
Homo sapiens
-
IC50: 0.0154 mM, competitive inhibition
0.0018
gabaculine
Rattus norvegicus
-
IC50: 0.0018 mM, potent and irreversible inhibitor
0.35
vigabatrin
Rattus norvegicus
-
IC50: 0.35 mM
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0183
isoform GABA-T2, using pyruvate as amino cceptor
0.0209
isoform GABA-T2, using glyoxylate as amino cceptor
0.1
substrate beta-alanine, discontinuous determination of glutamate using glutamate dehydrogenase in the presence of 10 M hydrazine, pH 8.0, 30C
0.13
substrate beta-alanine, continuous assay using malonic semialdehyde decarboxylase and alcohol dehydrogenase, pH 8.0, 30C
0.14
substrate beta-alanine, discontinuous determination of glutamate using glutamate dehydrogenase in the presence of 10 M hydrazine, pH 8.0, 30C
0.15
substrate beta-alanine, discontinuous determination of glutamate using glutamate dehydrogenase in the presence of 10 M hydrazine, pH 8.0, 30C
0.1785
isoform GABA-T3, using glyoxylate as amino acceptor
0.2065
isoform GABA-T3, using pyruvate as amino acceptor
0.75
substrate gamma-aminobutanoate, continuous assay with succinate dehydrogenase, pH 8.0, 30C
0.89
-
pH 8.5
1.6
substrate gamma-aminobutanoate, continuous assay using malonic semialdehyde decarboxylase and alcohol dehydrogenase, pH 8.0, 30C
1.8 - 3.5
-
37C
2.5
-
38C, pH 8.6
3.495
isoform GABA-T1, using glyoxylate as amino acceptor
3.52
substrate beta-alanine, discontinuous determination of glutamate using glutamate dehydrogenase in the presence of 10 M hydrazine, pH 8.0, 30C
3.6
substrate gamma-aminobutanoate, continuous assay using malonic semialdehyde decarboxylase and alcohol dehydrogenase, pH 8.0, 30C
3.67
continuous assay using malonic semialdehyde decarboxylase and alcohol dehydrogenase, pH 8.0, 30C
4.1
-
-
4.5
substrate beta-alanine, continuous assay using malonic semialdehyde decarboxylase and alcohol dehydrogenase, pH 8.0, 30C
5
-
37C, pH 8.0
5.79
isoform GABA-T1, using pyruvate as amino acceptor
10
-
38C, pH 8.5
12.8
substrate gamma-aminobutanoate, continuous assay with succinate dehydrogenase, pH 8.0, 30C; using 4-aminobutanoate as substrate
17.5
-
pH 8.4, 25C
18
-
pH 8.4, 25C
18.2
-
-
52.33
Candida guilliermondii var. membranaefaciens
-
-
170
-
pH 7.3, 37C, liver enzyme
260
-
pH 7.3, 37C, kidney enzyme
600
-
pH 7.3, 37C, brain enzyme
1736
pH 8.2, 30C
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
-
mitochondrial enzyme
7 - 8
-
mitochondrial enzyme
7.8 - 8
Candida guilliermondii var. membranaefaciens
-
at 35C
8.5 - 9
-
-
8.5 - 8.6
-
-
8.6 - 9
-
at 25C
8.6
-
assay at
8.8
-
isozyme I
additional information
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5
-
no activity below
6.5 - 11
-
no activity above or below
6.9 - 8.8
-
about half-maximal activity at pH 6.9 and 8.8
7 - 9
more than 70% of maximum activity
7.2 - 10
enzyme activity is detected
7.4 - 9
-
about half-maximal activity at pH 7.4 and about 90% of maximal activity at pH 9
8 - 9.2
8.5 - 9.5
maximal activity; maximal activity
11
-
and above, no activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 40
-
about half-maximal activity at 20C and 40C
20 - 50
-
linear increase, above 55C rapid decrease
25 - 45
Candida guilliermondii var. membranaefaciens
-
linear increase, above 45C rapid decrease
30 - 60
-
linear increase, above 65C rapid decrease
30 - 55
-
about half-maximal activity at 30C and 55C
35 - 70
-
about half-maximal activity at 35C and 70C
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.97
-
calculated from sequence of cDNA
6.33
calculated
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
20% of the activity in grey matter
Manually annotated by BRENDA team
highest expression in ripe fruit leaf at day 92; highest expression of in ripe fruit leaf at day 92; highest expression of in ripe fruit leaf at day 92
Manually annotated by BRENDA team
-
supraoesophageal
Manually annotated by BRENDA team
transcripts of gamma-aminobutanoate transaminase and glutamic acid decarboxylase 65, and glutamic acid decarboxylase 67 are detected throughout the brain, largely in the ventral and medial regions of telencephalon, nucleus preopticus, nucleus recessus lateralis of the hypothalamus, and Purkinje cell layer of the cerebellum
Manually annotated by BRENDA team
transcripts of gamma-aminobutanoate transaminase and glutamic acid decarboxylase 65, and glutamic acid decarboxylase 67 are detected throughout the brain, largely in the ventral and medial regions of telencephalon, nucleus preopticus, nucleus recessus lateralis of the hypothalamus, and Purkinje cell layer of the cerebellum
Manually annotated by BRENDA team
transcripts of gamma-aminobutanoate transaminase and glutamic acid decarboxylase 65, and glutamic acid decarboxylase 67 are detected throughout the brain, largely in the ventral and medial regions of telencephalon, nucleus preopticus, nucleus recessus lateralis of the hypothalamus, and Purkinje cell layer of the cerebellum
Manually annotated by BRENDA team
enzyme activity of gamma-aminobutanoate transaminase is highest in the hypothalamus and optic tectum
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Arthrobacter aurescens (strain TC1)
Arthrobacter aurescens (strain TC1)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Sulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45000
denaturated protein, determined by SDS-PAGE
53060
-
calculated from sequence of cDNA
54200
SDS-PAGE, single recombinant truncated MdGABA-T; SDS-PAGE, single recombinant truncated MdGABA-T
100000
103000
-
PAGE
105000
107000
109000
-
high speed sedimentation equilibrium centrifugation
110000
-
liver enzyme, gel filtration
110100
-
liver enzyme, crystallographic data
120000
mass of native protein, determined by gel filtration
176000 - 178000
-
gel filtration, sedimentation equilibrium centrifugation
200000
-
sucrose density gradient centrifugation
additional information
-
compared to liver enzyme, mature form of brain enzyme has an additional peptide at the N-terminus which may be cleaved by liver mitochondrial extract
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?