Information on EC 2.7.7.24 - glucose-1-phosphate thymidylyltransferase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

EC NUMBER
COMMENTARY
2.7.7.24
-
RECOMMENDED NAME
GeneOntology No.
glucose-1-phosphate thymidylyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
dTTP + alpha-D-glucose 1-phosphate = diphosphate + dTDP-alpha-D-glucose
show the reaction diagram
-
-
-
-
dTTP + alpha-D-glucose 1-phosphate = diphosphate + dTDP-alpha-D-glucose
show the reaction diagram
ordered bi-bi mechanism
-, Q9HU22
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
nucleotidyl group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
-
dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactose biosynthesis
-
dTDP-3-acetamido-3,6-dideoxy-alpha-D-glucose biosynthesis
-
dTDP-6-deoxy-alpha-D-allose biosynthesis
-
dTDP-alpha-D-mycaminose biosynthesis
-
dTDP-beta-L-4-epi-vancosamine biosynthesis
-
dTDP-beta-L-noviose biosynthesis
-
dTDP-D-beta-fucofuranose biosynthesis
-
dTDP-D-desosamine biosynthesis
-
dTDP-D-forosamine biosynthesis
-
dTDP-D-olivose, dTDP-D-oliose and dTDP-D-mycarose biosynthesis
-
dTDP-L-megosamine biosynthesis
-
dTDP-L-mycarose biosynthesis
-
dTDP-L-olivose biosynthesis
-
dTDP-L-rhamnose biosynthesis I
-
dTDP-L-rhamnose biosynthesis II
-
dTDP-N-acetylthomosamine biosynthesis
-
dTDP-N-acetylviosamine biosynthesis
-
Metabolic pathways
-
Polyketide sugar unit biosynthesis
-
Streptomycin biosynthesis
-
SYSTEMATIC NAME
IUBMB Comments
dTTP:alpha-D-glucose-1-phosphate thymidylyltransferase
Involved in the biosynthesis of L-rhamnose in bacteria.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
D-glucose-1-phosphate thymidylyltransferase
-
-
D-glucose-1-phosphate thymidylyltransferase
Mycobacterium smegmatis mc2155
-
-
-
dTDP-D-glucose synthase
-
-
dTDP-D-glucose synthase
Streptomyces sp. SCC-2136
-
-
-
dTDP-glucose synthase
-
-
-
-
dTDP-glucose-pyrophosphorylase
-
-
-
-
dTDPglucose pyrophosphorylase
-
-
-
-
Glc-1-P TTase
Q975F9
-
Glc-1-P TTase
Sulfolobus tokodaii 7
Q975F9
-
-
glucose 1-phosphate thymidylyltransferase
-
-
-
-
glucose-1-phosphate thymidylyltransferase
-
-
glucose-1-phosphate thymidylyltransferase
-
-
glucose-1-phosphate thymidylyltransferase
-
-
glucose-1-phosphate thymidylyltransferase
Q2HR55
-
glucose-1-phosphate thymidylyltransferase
Streptomyces sp. GERI-155
-
-
-
glucose-1-phosphate thymidylyltransferase
Streptomyces sp. SCC-2136
Q2HR55
-
-
glucose-1-phosphate thymidylyltransferase
Q400H3
-
RmlA
Mycobacterium smegmatis mc2155
-
-
-
RmlA
Salmonella enterica typhimurium
-
-
RmlA
Salmonella enterica typhimurium LT2
-
-
-
RmlA protein
-
protein encoded by rmlA gene is involved in gellan gum biosynthesis and exhibits both dTDP- and UDP-glucose diphosphorylase activities
SchS6
Streptomyces sp. SCC-2136
-
-
-
ST0452
Q975F9
locus name
ST0452
Sulfolobus tokodaii 7
Q975F9
locus name; locus name
-
sugar-1-phosphate nucleotidyltransferase
-
-
TDP-glucose pyrophosphorylase
-
-
-
-
thymidine diphosphate glucose pyrophosphorylase
-
-
-
-
thymidine diphosphoglucose pyrophosphorylase
-
-
-
-
thymidylyltransferase, glucose 1-phosphate
-
-
-
-
UgpG protein
-
protein encoded by ugpG gene is involved in gellan gum biosynthesis and exhibits both dTDP- and UDP-glucose diphosphorylase activities
CAS REGISTRY NUMBER
COMMENTARY
9026-03-3
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
ATCC 6633
-
-
Manually annotated by BRENDA team
ATCC 13027
-
-
Manually annotated by BRENDA team
germinated
-
-
Manually annotated by BRENDA team
Leuconostoc mesenteroides P-60
strain P-60
-
-
Manually annotated by BRENDA team
germinated
-
-
Manually annotated by BRENDA team
strain mc2155
-
-
Manually annotated by BRENDA team
Mycobacterium smegmatis mc2155
strain mc2155
-
-
Manually annotated by BRENDA team
-
Q9HU22
UniProt
Manually annotated by BRENDA team
Salmonella enterica typhimurium
strain LT2
-
-
Manually annotated by BRENDA team
Salmonella enterica typhimurium LT2
strain LT2
-
-
Manually annotated by BRENDA team
ATCC 31461
-
-
Manually annotated by BRENDA team
Streptococcus zymogenes
ATCC 10100
-
-
Manually annotated by BRENDA team
Streptomyces antibioticus Tu99
T99
-
-
Manually annotated by BRENDA team
GERI-155
-
-
Manually annotated by BRENDA team
strain SCC-2136
-
-
Manually annotated by BRENDA team
strain SCC-2136
UniProt
Manually annotated by BRENDA team
Streptomyces sp. GERI-155
GERI-155
-
-
Manually annotated by BRENDA team
Streptomyces sp. SCC-2136
strain SCC-2136
-
-
Manually annotated by BRENDA team
Streptomyces sp. SCC-2136
strain SCC-2136
UniProt
Manually annotated by BRENDA team
Sulfolobus tokodaii 7
-
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
malfunction
-
btrD mutant shows no production of any antibiotics under the standard fermentation conditions, while the complementation of this disruption by transformation recovers the antibiotic production
physiological function
-
btrD gene is involved in the butirosin biosynthesis
physiological function
-
the multifunctional enzyme is involved in biosynthesis of UDP-N-acetyl-alpha-D-glucosamine, an activated and essential form of N-acetyl-alpha-D-glucosamine that is an important component in the polysaccharide structure of most organisms
physiological function
Sulfolobus tokodaii 7
-
the multifunctional enzyme is involved in biosynthesis of UDP-N-acetyl-alpha-D-glucosamine, an activated and essential form of N-acetyl-alpha-D-glucosamine that is an important component in the polysaccharide structure of most organisms
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + alpha-D-glucose 1-phosphate
ADP-glucose + diphosphate
show the reaction diagram
Salmonella enterica typhimurium
-
-
-
-
?
ATP + alpha-D-glucose 1-phosphate
ADP-glucose + diphosphate
show the reaction diagram
Q2HR55, -
1% activity compared to dTTP
-
-
r
ATP + alpha-D-glucose 1-phosphate
ADP-glucose + diphosphate
show the reaction diagram
Streptomyces sp. SCC-2136
Q2HR55, -
1% activity compared to dTTP
-
-
r
ATP + alpha-D-glucose-1-phosphate
ADP-glucose + diphosphate
show the reaction diagram
Q2HR55, -
1.0% of the activity with dTTP and alpha-D-glucose 1-phosphate
-
-
?
ATP + alpha-D-glucose-1-phosphate
ADP-glucose + diphosphate
show the reaction diagram
Streptomyces sp. SCC-2136
Q2HR55, -
1.0% of the activity with dTTP and alpha-D-glucose 1-phosphate
-
-
?
CTP + alpha-D-glucose 1-phosphate
CDP-glucose + diphosphate
show the reaction diagram
Salmonella enterica typhimurium
-
-
-
-
?
CTP + alpha-D-glucose 1-phosphate
CDP-glucose + diphosphate
show the reaction diagram
Q2HR55, -
1.2% activity compared to dTTP
-
-
r
CTP + alpha-D-glucose-1-phosphate
CDP-glucose + diphosphate
show the reaction diagram
Q2HR55, -
1.2% of the activity with dTTP and alpha-D-glucose 1-phosphate
-
-
?
CTP + alpha-D-glucose-1-phosphate
CDP-glucose + diphosphate
show the reaction diagram
Streptomyces sp. SCC-2136
Q2HR55, -
1.2% of the activity with dTTP and alpha-D-glucose 1-phosphate
-
-
?
D-glucosamine 1-phosphate + TTP
TTP-D-glucosamine + diphosphate
show the reaction diagram
-
-
-
-
?
D-glucosamine 1-phosphate + UTP
UDP-D-glucosamine + diphosphate
show the reaction diagram
-
-
-
-
?
D-glucose 1-phosphate + UTP
UDP-D-glucose + diphosphate
show the reaction diagram
-
-
-
-
?
dATP + alpha-D-glucose 1-phosphate
dADP-glucose + diphosphate
show the reaction diagram
Salmonella enterica typhimurium, Salmonella enterica typhimurium LT2
-
-
-
-
?
dCTP + alpha-D-glucose 1-phosphate
dCDP-glucose + diphosphate
show the reaction diagram
Salmonella enterica typhimurium, Salmonella enterica typhimurium LT2
-
-
-
-
?
dGTP + alpha-D-glucose 1-phosphate
dGDP-glucose + diphosphate
show the reaction diagram
Salmonella enterica typhimurium, Salmonella enterica typhimurium LT2
-
-
-
-
?
dTDP-glucose + diphosphate
dTTP + alpha-D-glucose 1-phosphate
show the reaction diagram
-
-, protein by ugpG gene is involved in gellan gum biosynthesis and exhibits both dTDP- and UDP-glucose diphosphorylase activities, protein encoded by rmlA gene is involved in gellan gum biosynthesis and exhibits both dTDP- and UDP-glucose diphosphorylase activities
-
-
?
dTTP + alpha-D-galactose 1-phosphate
dTDP-galactose + diphosphate
show the reaction diagram
-
12.6% of the activity with alpha-D-glucose 1-phosphate
-
-
?
dTTP + alpha-D-galactose 1-phosphate
dTDP-galactose + diphosphate
show the reaction diagram
Q2HR55, -
10% activity compared to alpha-D-glucose 1-phosphate
-
-
r
dTTP + alpha-D-galactose 1-phosphate
dTDP-galactose + diphosphate
show the reaction diagram
Streptomyces sp. GERI-155
-
12.6% of the activity with alpha-D-glucose 1-phosphate
-
-
?
dTTP + alpha-D-galactose 1-phosphate
dTDP-galactose + diphosphate
show the reaction diagram
Streptomyces sp. SCC-2136
Q2HR55, -
10% activity compared to alpha-D-glucose 1-phosphate
-
-
r
dTTP + alpha-D-galactose-1-phosphate
dTDP-galactose + diphosphate
show the reaction diagram
Q2HR55, -
10% of the activity with dTTP and alpha-D-glucose 1-phosphate
-
-
?
dTTP + alpha-D-glucosamine 1-phosphate
dTDP-glucosamine + diphosphate
show the reaction diagram
Q2HR55, -
23% activity compared to alpha-D-glucose 1-phosphate
-
-
r
dTTP + alpha-D-glucosamine-1-phosphate
dTDP-glucosamine + diphosphate
show the reaction diagram
Q2HR55, -
23% of the activity with dTTP and alpha-D-glucose 1-phosphate
-
-
?
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
-
-
-
-
-
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
-
-
-
-
r
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
-
-
-
-
?
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
-
-
-
-
r
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
-
-
-
-
-
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
-
-
-
-
-
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
-
-
-
-
?
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
-
-
-
-
r
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
-
-
-
-
r
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
-
-
-
-
-
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
-
-
-
-
-
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
-
-
-
-
-
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
-
-
-
-
?
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
-
-
-
-
r
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
Streptococcus zymogenes
-
-
-
-
-
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
Q400H3, -
-
-
-
?
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
Salmonella enterica typhimurium
-
-
-
-
?
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
Q2HR55, -
-
highly specific for dTDP-glucose and diphosphate
-
r
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
Q2HR55, -
100% activity
-
-
r
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
-
sequential mechanism
-
-
?
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
-
probably involved in conversion of D-glucose to L-rhamnose in the biosynthesis of deoxy compound
-
-
-
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
-
first enzyme of the dTDP-L-rhamnose synthesis pathway
-
-
?
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
-
the enzyme catalyzes the first step in the biosynthesis of 4-deoxy-4-(dimethylamino)-5,5-dimethyl-D-ribopyranose moiety
-
-
?
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
-
in the opposite direction the enzyme is highly specific for dTDP-D-glucose and diphosphate
-
-
r
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
Leuconostoc mesenteroides P-60
-
-
-
-
-
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
Mycobacterium smegmatis mc2155
-
-
-
-
?
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
Salmonella enterica typhimurium LT2
-
-
-
-
?
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
Streptomyces antibioticus Tu99
-
-
-
-
r
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
Streptomyces sp. GERI-155
-
in the opposite direction the enzyme is highly specific for dTDP-D-glucose and diphosphate
-
-
r
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
Streptomyces sp. SCC-2136
Q2HR55, -
-
highly specific for dTDP-glucose and diphosphate
-
r
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
Streptomyces sp. SCC-2136
Q2HR55, -
100% activity
-
-
r
dTTP + alpha-D-glucose 1-phosphate
diphosphate + dTDP-glucose
show the reaction diagram
-
-
-
-
?
dTTP + alpha-D-glucose 1-phosphate
dTDP-alpha-D-glucose + diphosphate
show the reaction diagram
C6K8M0
-
-
-
?
dTTP + alpha-D-glucose 1-phosphate
diphosphate + dTDP-alpha-D-glucose
show the reaction diagram
Q975F9, -
maximal velocity of the reverse reaction is 11.4fold of the maximal velocity of the forward reaction. The multifunctional enzyme also shows activity with UTP + alpha-D-glucose 1-phosphate (EC 2.7.7.9, UTP-glucose-1-phosphate uridylyltransferase), UTP + N-acetylglucosamine 1-phosphate (EC 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase), dTTP + N-acetylglucosamine 1-phosphate (N-acetylglucosamine 1-phosphate thymidylyltransferase), dCTP + alpha-D-glucose 1-phosphate (glucose-1-phosphate cytidylyltransferase), dGTP + alpha-D-glucose 1-phosphate (glucose-1-phosphate guanylyltransferase), dATP + alpha-D-glucose 1-phosphate (glucose-1-phosphate adenylyltransferase). No activity with: alpha-D-glucose 1-phosphate + dATP, alpha-D-glucose 1-phosphate + dCTP, alpha-D-glucose 1-phosphate + dGTP, UTP + alpha-D-mannose1-phosphate, UTP + alpha-D-galactose 1-phosphate, UTP + alpha-D-glucosamine 1-phosphate
-
-
r
dTTP + alpha-D-glucose 1-phosphate
diphosphate + dTDP-alpha-D-glucose
show the reaction diagram
-
the enzyme also shows activity of EC 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase
-
-
?
dTTP + alpha-D-glucose 1-phosphate
diphosphate + dTDP-alpha-D-glucose
show the reaction diagram
Sulfolobus tokodaii 7
Q975F9
maximal velocity of the reverse reaction is 11.4fold of the maximal velocity of the forward reaction. The multifunctional enzyme also shows activity with UTP + alpha-D-glucose 1-phosphate (EC 2.7.7.9, UTP-glucose-1-phosphate uridylyltransferase), UTP + N-acetylglucosamine 1-phosphate (EC 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase), dTTP + N-acetylglucosamine 1-phosphate (N-acetylglucosamine 1-phosphate thymidylyltransferase), dCTP + alpha-D-glucose 1-phosphate (glucose-1-phosphate cytidylyltransferase), dGTP + alpha-D-glucose 1-phosphate (glucose-1-phosphate guanylyltransferase), dATP + alpha-D-glucose 1-phosphate (glucose-1-phosphate adenylyltransferase). No activity with: alpha-D-glucose 1-phosphate + dATP, alpha-D-glucose 1-phosphate + dCTP, alpha-D-glucose 1-phosphate + dGTP, UTP + alpha-D-mannose1-phosphate, UTP + alpha-D-galactose 1-phosphate, UTP + alpha-D-glucosamine 1-phosphate
-
-
r
dTTP + alpha-D-glucose 1-phosphate
diphosphate + dTDP-alpha-D-glucose
show the reaction diagram
Sulfolobus tokodaii 7
-
the enzyme also shows activity of EC 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase
-
-
?
dTTP + alpha-D-mannose 1-phosphate
dTDP-mannose + diphosphate
show the reaction diagram
Q2HR55, -
11% activity compared to alpha-D-glucose 1-phosphate
-
-
r
dTTP + alpha-D-mannose-1-phosphate
dTDP-mannose + diphosphate
show the reaction diagram
Q2HR55, -
11% of the activity with dTTP and alpha-D-glucose 1-phosphate
-
-
?
TTP + alpha-D-glucose 1-phosphate
TDP-glucose + diphosphate
show the reaction diagram
-
-
-
-
r
UTP + alpha-D-glucosamine 1-phosphate
UDP-glucosamine + diphosphate
show the reaction diagram
Q2HR55, -
8% activity compared to the reaction with alpha-D-glucose 1-phosphate and dTTP
-
-
r
UTP + alpha-D-glucosamine-1-phosphate
UDP-glucosamine + diphosphate
show the reaction diagram
Q2HR55, -
8% of the activity with dTTP and alpha-D-glucose 1-phosphate
-
-
?
UTP + alpha-D-glucose 1-phosphate
UDP-glucose + diphosphate
show the reaction diagram
-
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
UDP-glucose + diphosphate
show the reaction diagram
Salmonella enterica typhimurium
-
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
UDP-glucose + diphosphate
show the reaction diagram
-
5.5% of the activity with dTTP
-
-
?
UTP + alpha-D-glucose 1-phosphate
UDP-glucose + diphosphate
show the reaction diagram
Q2HR55, -
12% activity compared to dTTP
-
-
r
UTP + alpha-D-glucose 1-phosphate
UDP-glucose + diphosphate
show the reaction diagram
Streptomyces sp. GERI-155
-
5.5% of the activity with dTTP
-
-
?
UTP + alpha-D-glucose 1-phosphate
UDP-glucose + diphosphate
show the reaction diagram
Streptomyces sp. SCC-2136
Q2HR55, -
12% activity compared to dTTP
-
-
r
UTP + alpha-D-glucose-1-phosphate
UDP-glucose + diphosphate
show the reaction diagram
Q2HR55, -
12% of the activity with dTTP and alpha-D-glucose 1-phosphate
-
-
?
GTP + alpha-D-glucose 1-phosphate
GDP-glucose + diphosphate
show the reaction diagram
Salmonella enterica typhimurium
-
-
-
-
?
additional information
?
-
Q2HR55, -
GTP, UTP/alpha-D-mannose-1-phosphate or UTP/alpha-D-galactose-1-phosphate are no substrates
-
-
-
additional information
?
-
Q2HR55, -
the enzyme shows 102% relative activity for dTMP or dTDP compared to dTTP at 2 mM, respectively
-
-
-
additional information
?
-
Q2HR55, -
almost no activity with ATP-D-glucose, CTP-D-glucose, UTP-D-Glucose, GTP, alpha-D-galactose-1-phosphate, and alpha-D-mannose-1-phosphate
-
-
-
additional information
?
-
Salmonella enterica typhimurium
-
utilizes all eight naturally occurring NTPs with varying levels of catalytic efficiency, even in the presence of nonnative sugar-1-phosphates
-
-
-
additional information
?
-
-
BtrD is inactive with ATP, GTP, or CTP
-
-
-
additional information
?
-
-
the multifunctional enzyme is involved in biosynthesis of UDP-N-acetyl-alpha-D-glucosamine, an activated and essential form of N-acetyl-alpha-D-glucosamine that is an important component in the polysaccharide structure of most organisms
-
-
-
additional information
?
-
Salmonella enterica typhimurium LT2
-
utilizes all eight naturally occurring NTPs with varying levels of catalytic efficiency, even in the presence of nonnative sugar-1-phosphates
-
-
-
additional information
?
-
Sulfolobus tokodaii 7
-
the multifunctional enzyme is involved in biosynthesis of UDP-N-acetyl-alpha-D-glucosamine, an activated and essential form of N-acetyl-alpha-D-glucosamine that is an important component in the polysaccharide structure of most organisms
-
-
-
additional information
?
-
Streptomyces sp. SCC-2136
Q2HR55, -
GTP, UTP/alpha-D-mannose-1-phosphate or UTP/alpha-D-galactose-1-phosphate are no substrates
-
-
-
additional information
?
-
Streptomyces sp. SCC-2136
Q2HR55, -
the enzyme shows 102% relative activity for dTMP or dTDP compared to dTTP at 2 mM, respectively
-
-
-
additional information
?
-
Streptomyces sp. SCC-2136
Q2HR55, -
almost no activity with ATP-D-glucose, CTP-D-glucose, UTP-D-Glucose, GTP, alpha-D-galactose-1-phosphate, and alpha-D-mannose-1-phosphate
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dTDP-glucose + diphosphate
dTTP + alpha-D-glucose 1-phosphate
show the reaction diagram
-
protein by ugpG gene is involved in gellan gum biosynthesis and exhibits both dTDP- and UDP-glucose diphosphorylase activities, protein encoded by rmlA gene is involved in gellan gum biosynthesis and exhibits both dTDP- and UDP-glucose diphosphorylase activities
-
-
?
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
-
-
-
-
-
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
-
-
-
-
r
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
-
-
-
-
r
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
-
-
-
-
-
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
-
-
-
-
-
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
-
-
-
-
r
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
-
-
-
-
r
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
-
-
-
-
-
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
-
-
-
-
-
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
-
-
-
-
-
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
-
-
-
-
r
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
Streptococcus zymogenes
-
-
-
-
-
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
-
first enzyme of the dTDP-L-rhamnose synthesis pathway
-
-
?
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
-
the enzyme catalyzes the first step in the biosynthesis of 4-deoxy-4-(dimethylamino)-5,5-dimethyl-D-ribopyranose moiety
-
-
?
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
Leuconostoc mesenteroides P-60
-
-
-
-
-
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
Streptomyces antibioticus Tu99
-
-
-
-
r
additional information
?
-
Sulfolobus tokodaii, Sulfolobus tokodaii 7
-
the multifunctional enzyme is involved in biosynthesis of UDP-N-acetyl-alpha-D-glucosamine, an activated and essential form of N-acetyl-alpha-D-glucosamine that is an important component in the polysaccharide structure of most organisms
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ca2+
-
5.5 mM, 2.1% activity
Ca2+
-
2 mM. Activation of UDP-N-acetylglucosamine diphosphorylase activity in the order of decreasing effectiveness: Co2+, Mn2+, Mg2+, Zn2+, Ca2+. No activity is detectable without a divalent cation
Co2+
-
absolute requirement for divalent cation. Order of decreasing effectiveness is: Co2+, Mn2+, Mg2+, Zn2+
Co2+
-
absolute requirement for a divalent metal ion. Order of effectiveness is: Mn2+, Mg2+, Zn2+, Cu2+, Fe2+, Ni2+, Co2+
Co2+
-
5.5 mM, 124% activity
Co2+
-
2 mM. Activation of UDP-N-acetylglucosamine diphosphorylase activity in the order of decreasing effectiveness: Co2+, Mn2+, Mg2+, Zn2+, Ca2+. No activity is detectable without a divalent cation
Cu2+
-
absolute requirement for a divalent metal ion. Order of effectiveness is: Mn2+, Mg2+, Zn2+, Cu2+, Fe2+, Ni2+, Co2+
Cu2+
-
5.5 mM, 0.6% activity
Fe2+
-
absolute requirement for a divalent metal ion. Order of effectiveness is: Mn2+, Mg2+, Zn2+, Cu2+, Fe2+, Ni2+, Co2+
Fe2+
-
5.5 mM, 0.3% activity
Mg2+
-
absolute requirement, 2 mM: optimal
Mg2+
-
absolute requirement, 2 mM: optimal
Mg2+
-
absolute requirement for divalent cation. Order of decreasing effectiveness is: Co2+, Mn2+, Mg2+, Zn2+. Optimal Mg2+ concentration is 6 mM
Mg2+
-
absolute requirement for a divalent metal ion. Order of effectiveness is: Mn2+, Mg2+, Zn2+, Cu2+, Fe2+, Ni2+, Co2+
Mg2+
-
5.5 mM, 100% activity
Mg2+
-
required, optimal concentration 5 mM
Mg2+
-
2 mM. Activation of UDP-N-acetylglucosamine diphosphorylase activity in the order of decreasing effectiveness: Co2+, Mn2+, Mg2+, Zn2+, Ca2+. No activity is detectable without a divalent cation
Mn2+
-
absolute requirement for divalent cation. Order of decreasing effectiveness is: Co2+, Mn2+, Mg2+, Zn2+
Mn2+
-
absolute requirement for a divalent metal ion. Order of effectiveness is: Mn2+, Mg2+, Zn2+, Cu2+, Fe2+, Ni2+, Co2+
Mn2+
-
5.5 mM, 98.5% activity
Mn2+
-
2 mM. Activation of UDP-N-acetylglucosamine diphosphorylase activity in the order of decreasing effectiveness: Co2+, Mn2+, Mg2+, Zn2+, Ca2+. No activity is detectable without a divalent cation
Ni2+
-
absolute requirement for a divalent metal ion. Order of effectiveness is: Mn2+, Mg2+, Zn2+, Cu2+, Fe2+, Ni2+, Co2+
Ni2+
-
5.5 mM, 97.1% activity
Zn2+
-
absolute requirement for divalent cation. Order of decreasing effectiveness is: Co2+, Mn2+, Mg2+, Zn2+
Zn2+
-
absolute requirement for a divalent metal ion. Order of effectiveness is: Mn2+, Mg2+, Zn2+, Cu2+, Fe2+, Ni2+, Co2+
Zn2+
-
5.5 mM, 17.9% activity
Zn2+
-
2 mM. Activation of UDP-N-acetylglucosamine diphosphorylase activity in the order of decreasing effectiveness: Co2+, Mn2+, Mg2+, Zn2+, Ca2+. No activity is detectable without a divalent cation
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
dTDP-glucose
-
competitive to dTTP
dTDP-L-rhamnose
-
-
dTTP
-
competitive to dTDPglucose and non-competitive to alpha-D-glucose 1-phosphate
N-(6-amino-1-benzyl-1,2,3,4-tetrahydropyrimidin-5-yl)-N-methylbenzenesulfonamide
-, Q9HU22
inhibitor does not bind at the active site of RmlA but binds at a second site remote from the active site. Despite this, the compound acts as competitive inhibitor but with high cooperativity
N-(6-amino-1-benzyl-1,2,3,4-tetrahydropyrimidin-5-yl)-N-methylbutane-1-sulfonamide
-, Q9HU22
inhibitor does not bind at the active site of RmlA but binds at a second site remote from the active site. Despite this, the compound acts as competitive inhibitor but with high cooperativity
N-(6-amino-1-benzyl-1,2,3,4-tetrahydropyrimidin-5-yl)-N-methylfuran-2-sulfonamide
-, Q9HU22
inhibitor does not bind at the active site of RmlA but binds at a second site remote from the active site. Despite this, the compound acts as competitive inhibitor but with high cooperativity
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.034
-
alpha-D-glucose 1-phosphate
-
37C pH 8
0.069
-
alpha-D-glucose 1-phosphate
-
pH 7.5, 37C
0.8
-
alpha-D-glucose 1-phosphate
-
37C pH 8
1.12
-
alpha-D-glucose 1-phosphate
-
pH 7.5, 80C
3.4
-
ATP
Salmonella enterica typhimurium
-
apparent value, mutant enzyme Q83S, in 100 mM Tris-HCl, pH 8.0, at 37C; mutant enzyme Q83S, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37C
9
-
ATP
Salmonella enterica typhimurium
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37C; wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37C
13.7
-
CTP
Salmonella enterica typhimurium
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37C; wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37C
1.1
-
dATP
Salmonella enterica typhimurium
-
apparent value, mutant enzyme Q83S, in 100 mM Tris-HCl, pH 8.0, at 37C; mutant enzyme Q83S, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37C
7.8
-
dATP
Salmonella enterica typhimurium
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37C; wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37C
10.5
-
dCTP
Salmonella enterica typhimurium
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37C; wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37C
3.7
-
dGTP
Salmonella enterica typhimurium
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37C; wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37C
11.5
-
dGTP
Salmonella enterica typhimurium
-
apparent value, mutant enzyme Q83D, in 100 mM Tris-HCl, pH 8.0, at 37C; mutant enzyme Q83D, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37C
0.154
-
diphosphate
-
37C pH 8
0.39
-
diphosphate
-
pH 7.5, 80C
0.05
-
dTDP-alpha-D-glucose
-
pH 7.5, 80C
0.0094
-
dTDP-glucose
-
UgpG protein
0.012
-
dTDP-glucose
-
RmlA protein
0.095
-
dTDP-glucose
-
37C pH 8
0.02
-
dTTP
-
pH 7.5, 80C
0.02
-
dTTP
-
pH 7.5, 37C
0.0205
-
dTTP
-
37C pH 8
0.29
-
dTTP
Salmonella enterica typhimurium
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37C; wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37C
1.66
-
dTTP
C6K8M0
pH 7.2, 37C, fusion protein of isoform DnmL with C-terminal sequence of isoform RmbA
1.72
-
dTTP
C6K8M0
pH 7.2, 37, mutant R313S/T317V/H322A/A339S/S341H/D348H
2.4
-
dTTP
Salmonella enterica typhimurium
-
apparent value, mutant enzyme Q83S, in 100 mM Tris-HCl, pH 8.0, at 37C; mutant enzyme Q83S, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37C
6.2
-
dTTP
Salmonella enterica typhimurium
-
apparent value, mutant enzyme Q83D, in 100 mM Tris-HCl, pH 8.0, at 37C; mutant enzyme Q83D, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37C
1.3
-
GTP
Salmonella enterica typhimurium
-
apparent value, mutant enzyme Q83D, in 100 mM Tris-HCl, pH 8.0, at 37C; mutant enzyme Q83D, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37C
8.5
-
GTP
Salmonella enterica typhimurium
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37C; wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37C
0.05
-
TDP-glucose
-
25C, pH 8
0.1
-
TTP
-
25C, pH 8
1.2
-
UTP
-
wild type enzyme
4.6
-
UTP
Salmonella enterica typhimurium
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37C; wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37C
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.06
-
ATP
Salmonella enterica typhimurium
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37C; wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37C
0.48
-
ATP
Salmonella enterica typhimurium
-
apparent value, mutant enzyme Q83S, in 100 mM Tris-HCl, pH 8.0, at 37C; mutant enzyme Q83S, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37C
0.098
-
CTP
Salmonella enterica typhimurium
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37C
0.1
-
CTP
Salmonella enterica typhimurium
-
wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37C
1.88
-
dATP
Salmonella enterica typhimurium
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37C; wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37C
5.33
-
dATP
Salmonella enterica typhimurium
-
apparent value, mutant enzyme Q83S, in 100 mM Tris-HCl, pH 8.0, at 37C; mutant enzyme Q83S, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37C
1.22
-
dCTP
Salmonella enterica typhimurium
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37C; wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37C
2.12
-
dGTP
Salmonella enterica typhimurium
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37C; wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37C
5
-
dGTP
Salmonella enterica typhimurium
-
apparent value, mutant enzyme Q83D, in 100 mM Tris-HCl, pH 8.0, at 37C; mutant enzyme Q83D, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37C
0.56
-
dTDP-glucose
-
UgpG protein
1.35
-
dTDP-glucose
-
RmlA protein
3.13
-
dTTP
Salmonella enterica typhimurium
-
mutant enzyme Q83D, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37C
3.62
-
dTTP
Salmonella enterica typhimurium
-
apparent value, mutant enzyme Q83D, in 100 mM Tris-HCl, pH 8.0, at 37C
6.33
-
dTTP
Salmonella enterica typhimurium
-
apparent value, mutant enzyme Q83S, in 100 mM Tris-HCl, pH 8.0, at 37C; mutant enzyme Q83S, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37C
15
-
dTTP
Salmonella enterica typhimurium
-
wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37C
30.83
-
dTTP
Salmonella enterica typhimurium
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37C
0.14
-
GTP
Salmonella enterica typhimurium
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37C; wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37C
0.21
-
GTP
Salmonella enterica typhimurium
-
apparent value, mutant enzyme Q83D, in 100 mM Tris-HCl, pH 8.0, at 37C; mutant enzyme Q83D, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37C
24
-
UTP
-
wild type enzyme
41
-
UTP
Salmonella enterica typhimurium
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37C; wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37C
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1.1
-
dTTP
C6K8M0
pH 7.2, 37, mutant R313S/T317V/H322A/A339S/S341H/D348H
10218
1.15
-
dTTP
C6K8M0
pH 7.2, 37C, fusion protein of isoform DnmL with C-terminal sequence of isoform RmbA
10218
IC50 VALUE [mM]
IC50 VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.000073
-
N-(6-amino-1-benzyl-1,2,3,4-tetrahydropyrimidin-5-yl)-N-methylbenzenesulfonamide
-, Q9HU22
pH not specified in the publication, temperature not specified in the publication
0.000175
-
N-(6-amino-1-benzyl-1,2,3,4-tetrahydropyrimidin-5-yl)-N-methylbutane-1-sulfonamide
-, Q9HU22
pH not specified in the publication, temperature not specified in the publication
0.000105
-
N-(6-amino-1-benzyl-1,2,3,4-tetrahydropyrimidin-5-yl)-N-methylfuran-2-sulfonamide
-, Q9HU22
pH not specified in the publication, temperature not specified in the publication
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.00085
-
Q2HR55, -
crude extract, at pH 8.0 and 37C
0.00166
-
-
-
0.004
-
Q2HR55, -
after 4.7fold purification, at pH 8.0 and 37C
0.017
-
Q2HR55, -
crude cell extract
0.0801
-
Q2HR55, -
after 4.7fold purification
1.35
-
-
pH 7.5, 80C, substrates: dTTP + alpha-D-glucose 1-phosphate
9.4
-
-
pH 7.5, 80C, substrates: diphosphate + dTDP-alpha-D-glucose
44.8
-
-
dTDPglucose + diphosphate
194
-
-
forward reaction
360
-
-
reverse reaction
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.5
-
-
assay at
7.5
-
-
assay at
7.5
-
-
assay at
8
8.5
-
broad
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6
10
-
pH 6.0: about 50% of maximal activity, pH 10.0: about 60% of maximal activity
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
25
-
-
assay at
25
-
-
assay at, dTDP-glucose + diphosphate
37
-
-
assay at, dTTP + alpha-D-glucose 1-phosphate
37
-
-
assay at
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
70
100
-
70C: about 40% of maximal activity, 100C: about 80% of maximal activity
PDB
SCOP
CATH
ORGANISM
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Salmonella paratyphi B (strain ATCC BAA-1250 / SPB7)
Salmonella paratyphi B (strain ATCC BAA-1250 / SPB7)
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
31360
-
-
calculated from amino acid sequence
31810
-
-
mass spectroscopy
33770
-
-
calculated from nucleic acid sequence
33800
-
-
MALDI mass spectrum
43000
-
-
gel filtration
48000
-
-
gel filtration
56000
-
Q2HR55, -
SDS-PAGE; SDS-PAGE
106000
122000
-
dynamic light scattering
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 30000, SDS-PAGE
?
-
x * 33700, SDS-PAGE, x * 33670, calculated
?
Streptomyces sp. GERI-155
-
x * 30000, SDS-PAGE
-
monomer
-
1 * 43000, SDS-PAGE
tetramer
-
alpha4, crystallization studies
tetramer
-
each monomer consisting of three functional subunits
monomer
Streptomyces antibioticus Tu99
-
1 * 43000, SDS-PAGE
-
additional information
-
trimer or tetramer
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
co-crystallization with dTDP-glucose or dTMP and alpha-D-glucose 1-phosphate or deoxythymidine and alpha-D-glucose 1-phosphate, hanging-drop vapour-diffusion method in 1.7 M ammonium sulfate and 0.1 M sodium acetate, pH 5.3
-
sitting-drop vapour-diffusion method in polyethylene glycol 6000, 0.1 M sodium citrate, pH 4.6 and 0.5 M Li2SO4 as precipitant, crystallization as apoenzyme or co-crystallization with dTTP or thymidine and alpha-D-glucose 1-phosphate or dTDP-D-glucose or dTDP-L-rhamnose
-
sitting-drop vapour-diffusion method in polyethylene glycol 6000, 0.1 M sodium citrate, pH 4.6 and 0.5 M Li2SO4 as precipitant, space group: P1, the asymmetric unit contains eight monomers in the form of two RmlA tetramers with a solvent content of 51%; sitting-drop vapour-diffusion technique with PEG 6000 and lithium sulfate as precipitant. Several diffraction data sets of single frozen crystals are collected to a resolution of 1.66 A. Crystals belong to space group P1, with unit-cell parameters a = 71.5 A, b = 73.1 A, c = 134.7 A, alpha = 89.9, beta = 80.9, gamma = 81
-
homology modeling of both isoforms DnmL and RmbA
C6K8M0
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
70
-
Q400H3, -
thermostable up to
80
-
-
half-life: 180 min
95
-
-
half-life: 60 min
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20C at concentrations higher than 5 mg/ml stable for up to three months
-
stable for several weeks at any step of purification if kept frozen
-
-80 C, 50 mM potassium phosphate buffer, pH 7.0, containing 1 mM DTT and 20% glycerol, purified SgcA1 is stable for several months with minimal loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
ATCC 6633, partial
-
ATCC 13027, partial
-
recombinant enzyme
-
germinated, partial
-
ATCC 17-5, partial
-
strain P-60, partial
-
germinated, partial
-
; recombinant enzyme with his-tag
-
ATCC 7700, partial
-
Ni-NTA agarose resin chromatography; PD-10 gel filtration, nickel-nitrilotriacetic acid agarose resin or spin column chromatography
Salmonella enterica typhimurium
-
recombinant enzyme, RmlA protein; recombinant enzyme, UgpG protein
-
ATCC 10100, partial
Streptococcus zymogenes
-
ammonium sulfate precipitation and Ni-NTA affinity column chromatography; ammonium sulfate precipitation and Ni-NTA affinity column chromatography
Q2HR55, -
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expression in Escherichia coli
-
expressed in Escherichia coli
-
expression in Escherichia coli as GST-fusion protein
-
expressed in Escherichia coli
-
expression in Escherichia coli
-
overexpression in Escherichia coli BL21 with a 6 x His-tag
-
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli BL21 (DE3) cells; expressed in Escherichia coli BL21(DE3) cells
Salmonella enterica typhimurium
-
expression in Escherichia coli, RmlA protein; expression in Escherichia coli, UgpG protein
-
overexpression in Escherichia coli
-
overexpression in Escherichia coli
-
expression in Escherichia coli, protein forms inclusion bodies
C6K8M0
expressed in Escherichia coli vector pSCHS6; expressed in Escherichia coli, vector pSCHS6
Q2HR55, -
overexpression in Escherichia coli
-
expression in Escherichia coli, wild-type and truncated enzyme form lacking the 170-residues C-terminal domain
-
Escherichia coli BL21(DE3)/pNOS110 produces protein mostly in an insoluble form under normal culture conditions
Q400H3, -
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Q83A
Salmonella enterica typhimurium
-
0.2% relative activity compared to the wild type enzyme, using dTTP as a substrate; reduced activity with dTTP and increased activity with dATP and ATP compared to the wild type enzyme
Q83D
Salmonella enterica typhimurium
-
0.2% relative activity compared to the wild type enzyme, using dTTP as a substrate; reduced activity with dTTP and increased activity with dGTP and GTP compared to the wild type enzyme
Q83E
Salmonella enterica typhimurium
-
1.0% relative activity compared to the wild type enzyme, using dTTP as a substrate; wild type activity with dTTP
Q83N
Salmonella enterica typhimurium
-
0.3% relative activity compared to the wild type enzyme, using dTTP as a substrate; reduced activity with dTTP and increased activity with dATP and ATP compared to the wild type enzyme
Q83S
Salmonella enterica typhimurium
-
0.3% relative activity compared to the wild type enzyme, using dTTP as a substrate; reduced activity with dTTP and increased activity with dATP and ATP compared to the wild type enzyme
Q83D
Salmonella enterica typhimurium LT2
-
0.2% relative activity compared to the wild type enzyme, using dTTP as a substrate; reduced activity with dTTP and increased activity with dGTP and GTP compared to the wild type enzyme
-
Q83N
Salmonella enterica typhimurium LT2
-
0.3% relative activity compared to the wild type enzyme, using dTTP as a substrate
-
R313S/T317V/H322A/A339S/S341H/D348H
-
mutation of residues to the corresponing residues of isoform RmbA, giving rise to highly similar hydrophobicity pattern with RmbA. Mutations render the protein expressed in soluble form, the mutant is catalytically active
-
D208A
-
UDP-N-acetylglucosamine diphosphorylase activity is very low
E146A
-
UDP-N-acetylglucosamine diphosphorylase activity is very low
G9A
-
UDP-N-acetylglucosamine diphosphorylase activity is very low
R13A
-
UDP-N-acetylglucosamine diphosphorylase activity is very low
T80A
-
enhanced UDP-N-acetylglucosamine diphosphorylase activity under optimal conditions
Y97A
-
enhanced UDP-N-acetylglucosamine diphosphorylase activity under optimal conditions
Q83S
Salmonella enterica typhimurium LT2
-
0.3% relative activity compared to the wild type enzyme, using dTTP as a substrate; reduced activity with dTTP and increased activity with dATP and ATP compared to the wild type enzyme
-
additional information
C6K8M0
deletion of the C-terminal sequence of isoform DnmL increases the expression level of truncated DnmL, albeit without enzymic activity. Substitution of the C-terminal sequence of DnmL with that of isoform RmbA also leads to expression of the recombinant protein in soluble form. The fusion protein is catalytically active
R313S/T317V/H322A/A339S/S341H/D348H
C6K8M0
mutation of residues to the corresponing residues of isoform RmbA, giving rise to highly similar hydrophobicity pattern with RmbA. Mutations render the protein expressed in soluble form, the mutant is catalytically active
additional information
-
deletion of the C-terminal sequence of isoform DnmL increases the expression level of truncated DnmL, albeit without enzymic activity. Substitution of the C-terminal sequence of DnmL with that of isoform RmbA also leads to expression of the recombinant protein in soluble form. The fusion protein is catalytically active
-
K147A
-
UDP-N-acetylglucosamine diphosphorylase activity is very low
additional information
-
a truncated enzyme form lacking the 170-residues C-terminal domain has decreased thermostability
Y97F
-
UDP-N-acetylglucosamine diphosphorylase activity is very low
additional information
Sulfolobus tokodaii 7
-
a truncated enzyme form lacking the 170-residues C-terminal domain has decreased thermostability
-
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
medicine
-
RmlA is a potential target for the development of new antituberculosis drugs
medicine
Mycobacterium smegmatis mc2155
-
RmlA is a potential target for the development of new antituberculosis drugs
-
analysis
-
microtiter platebased colorimetric assay for RmlA enzyme activity, based on hydrolysis of the diphosphate formed and detection of phosphate by malachite green reagent