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ATP + alpha-D-glucose 1-phosphate
ADP-glucose + diphosphate
ATP + alpha-D-glucose 1-phosphate
diphosphate + ADP-alpha-D-glucose
ATP + alpha-D-glucose-1-phosphate
ADP-glucose + diphosphate
CTP + alpha-D-glucose 1-phosphate
CDP-glucose + diphosphate
CTP + alpha-D-glucose 1-phosphate
diphosphate + CDP-alpha-D-glucose
CTP + alpha-D-glucose-1-phosphate
CDP-glucose + diphosphate
D-glucosamine 1-phosphate + TTP
TTP-D-glucosamine + diphosphate
-
-
-
-
?
D-glucosamine 1-phosphate + UTP
UDP-D-glucosamine + diphosphate
-
-
-
-
?
D-glucose 1-phosphate + UTP
UDP-D-glucose + diphosphate
-
-
-
-
?
dATP + alpha-D-glucosamine 1-phosphate
diphosphate + dADP-alpha-D-glucosamine
-
21% yield with 0.06 mM enzyme
-
-
?
dATP + alpha-D-glucose 1-phosphate
dADP-glucose + diphosphate
dCTP + alpha-D-glucose 1-phosphate
dCDP-glucose + diphosphate
dCTP + alpha-D-glucose 1-phosphate
diphosphate + dCDP-alpha-D-glucose
-
35% yield with 0.006 mM enzyme, 87% yield with 0.06 mM enzyme
-
-
?
deoxythymidine 5'-tetraphosphate + alpha-D-glucose 1-phosphate
triphosphate + dTDP-alpha-D-glucose
-
-
alternative products dTTP-alpha-D-glucose and diphosphate are not detected
-
?
dGTP + alpha-D-glucose 1-phosphate
dGDP-glucose + diphosphate
dGTP + alpha-D-glucose 1-phosphate
diphosphate + dGDP-alpha-D-glucose
-
53% yield with 0.006 mM enzyme, 89% yield with 0.06 mM enzyme
-
-
?
diphosphate + dTDP-alpha-D-glucose
dTTP + alpha-D-glucose 1-phosphate
-
-
-
r
dTDP-glucose + diphosphate
dTTP + alpha-D-glucose 1-phosphate
dTTP + 2-deoxy-2-amino-alpha-D-glucose 1-phosphate
diphosphate + dTDP-2-deoxy-2-amino-alpha-D-glucose
-
-
-
-
?
dTTP + 3-deoxy-3-amino-alpha-D-glucose 1-phosphate
diphosphate + dTDP-3-deoxy-3-amino-alpha-D-glucose
-
-
-
-
?
dTTP + 3-deoxy-3-azido-alpha-D-glucose 1-phosphate
diphosphate + dTDP-3-deoxy-3-azido-alpha-D-glucose
-
-
-
-
?
dTTP + alpha-D-galactosamine 1-phosphate
diphosphate + dTDP-alpha-D-galactosamine
-
5% yield with 0.06 mM enzyme
-
-
?
dTTP + alpha-D-galactose 1-phosphate
diphosphate + dTDP-alpha-D-galactose
-
91% yield with 0.06 mM enzyme
-
-
?
dTTP + alpha-D-galactose 1-phosphate
dTDP-galactose + diphosphate
dTTP + alpha-D-galactose-1-phosphate
dTDP-galactose + diphosphate
10% of the activity with dTTP and alpha-D-glucose 1-phosphate
-
-
?
dTTP + alpha-D-glucosamine 1-phosphate
diphosphate + dTDP-alpha-D-glucosamine
-
71% yield with 0.06 mM enzyme
-
-
?
dTTP + alpha-D-glucosamine 1-phosphate
dTDP-glucosamine + diphosphate
23% activity compared to alpha-D-glucose 1-phosphate
-
-
r
dTTP + alpha-D-glucosamine-1-phosphate
dTDP-glucosamine + diphosphate
23% of the activity with dTTP and alpha-D-glucose 1-phosphate
-
-
?
dTTP + alpha-D-glucose 1-phosphate
diphosphate + dTDP-alpha-D-glucose
dTTP + alpha-D-glucose 1-phosphate
diphosphate + dTDP-glucose
-
-
-
-
?
dTTP + alpha-D-glucose 1-phosphate
dTDP-alpha-D-glucose + diphosphate
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
dTTP + alpha-D-mannose 1-phosphate
diphosphate + dTDP-alpha-D-mannose
-
88% yield with 0.06 mM enzyme
-
-
?
dTTP + alpha-D-mannose 1-phosphate
dTDP-mannose + diphosphate
11% activity compared to alpha-D-glucose 1-phosphate
-
-
r
dTTP + alpha-D-mannose-1-phosphate
dTDP-mannose + diphosphate
11% of the activity with dTTP and alpha-D-glucose 1-phosphate
-
-
?
dTTP + [[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl]methyl]phosphonic acid
diphosphate + dTDP-[[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl]methyl]phosphonic acid
-
-
-
-
?
dUTP + alpha-D-galactose 1-phosphate
diphosphate + dUDP-alpha-D-galactose
-
83% yield with 0.06 mM enzyme
-
-
?
dUTP + alpha-D-glucose 1-phosphate
diphosphate + dUDP-alpha-D-glucose
-
88% yield with 0.006 mM enzyme, 95% yield with 0.06 mM enzyme
-
-
?
GTP + alpha-D-glucose 1-phosphate
diphosphate + GDP-alpha-D-glucose
GTP + alpha-D-glucose 1-phosphate
GDP-glucose + diphosphate
-
-
-
-
?
GTP + alpha-D-mannose 1-phosphate
diphosphate + GDP-alpha-D-mannose
-
13% yield with 0.06 mM enzyme
-
-
?
TTP + alpha-D-glucose 1-phosphate
TDP-glucose + diphosphate
-
-
-
-
r
UTP + 2-deoxy-2-amino-alpha-D-glucose 1-phosphate
diphosphate + UDP-2-deoxy-2-amino-alpha-D-glucose
-
-
-
-
?
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-alpha-D-galactose
-
86% yield with 0.06 mM enzyme
-
-
?
UTP + alpha-D-glucosamine 1-phosphate
diphosphate + UDP-alpha-D-glucosamine
-
12% yield with 0.06 mM enzyme
-
-
?
UTP + alpha-D-glucosamine 1-phosphate
UDP-glucosamine + diphosphate
8% activity compared to the reaction with alpha-D-glucose 1-phosphate and dTTP
-
-
r
UTP + alpha-D-glucosamine-1-phosphate
UDP-glucosamine + diphosphate
8% of the activity with dTTP and alpha-D-glucose 1-phosphate
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-alpha-D-glucose
UTP + alpha-D-glucose 1-phosphate
UDP-glucose + diphosphate
UTP + alpha-D-glucose-1-phosphate
UDP-glucose + diphosphate
12% of the activity with dTTP and alpha-D-glucose 1-phosphate
-
-
?
UTP + alpha-D-mannose 1-phosphate
diphosphate + UDP-alpha-D-mannose
-
78% yield with 0.06 mM enzyme
-
-
?
UTP + [[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl]methyl]phosphonic acid
diphosphate + UMP-[[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl]methyl]phosphonic acid
-
-
-
-
?
additional information
?
-
ATP + alpha-D-glucose 1-phosphate
ADP-glucose + diphosphate
-
-
-
-
?
ATP + alpha-D-glucose 1-phosphate
ADP-glucose + diphosphate
1% activity compared to dTTP
-
-
r
ATP + alpha-D-glucose 1-phosphate
ADP-glucose + diphosphate
1% activity compared to dTTP
-
-
r
ATP + alpha-D-glucose 1-phosphate
diphosphate + ADP-alpha-D-glucose
RML-1 demonstrates a broad substrate range toward five nucleotides (dTTP, UTP, CTP, ATP and GTP) in the presence of alpha-D-glucose 1-phosphate, with higher activity toward dTTP and UTP
-
-
?
ATP + alpha-D-glucose 1-phosphate
diphosphate + ADP-alpha-D-glucose
-
-
-
-
?
ATP + alpha-D-glucose 1-phosphate
diphosphate + ADP-alpha-D-glucose
-
13% yield with 0.006 mM enzyme, 78% yield with 0.06 mM enzyme
-
-
?
ATP + alpha-D-glucose-1-phosphate
ADP-glucose + diphosphate
1.0% of the activity with dTTP and alpha-D-glucose 1-phosphate
-
-
?
ATP + alpha-D-glucose-1-phosphate
ADP-glucose + diphosphate
1.0% of the activity with dTTP and alpha-D-glucose 1-phosphate
-
-
?
CTP + alpha-D-glucose 1-phosphate
CDP-glucose + diphosphate
-
-
-
-
?
CTP + alpha-D-glucose 1-phosphate
CDP-glucose + diphosphate
1.2% activity compared to dTTP
-
-
r
CTP + alpha-D-glucose 1-phosphate
diphosphate + CDP-alpha-D-glucose
RML-1 demonstrates a broad substrate range toward five nucleotides (dTTP, UTP, CTP, ATP and GTP) in the presence of alpha-D-glucose 1-phosphate, with higher activity toward dTTP and UTP
-
-
?
CTP + alpha-D-glucose 1-phosphate
diphosphate + CDP-alpha-D-glucose
-
-
-
-
?
CTP + alpha-D-glucose-1-phosphate
CDP-glucose + diphosphate
1.2% of the activity with dTTP and alpha-D-glucose 1-phosphate
-
-
?
CTP + alpha-D-glucose-1-phosphate
CDP-glucose + diphosphate
1.2% of the activity with dTTP and alpha-D-glucose 1-phosphate
-
-
?
dATP + alpha-D-glucose 1-phosphate
dADP-glucose + diphosphate
-
-
-
-
?
dATP + alpha-D-glucose 1-phosphate
dADP-glucose + diphosphate
Salmonella enterica typhimurium LT2
-
-
-
-
?
dCTP + alpha-D-glucose 1-phosphate
dCDP-glucose + diphosphate
-
-
-
-
?
dCTP + alpha-D-glucose 1-phosphate
dCDP-glucose + diphosphate
Salmonella enterica typhimurium LT2
-
-
-
-
?
dGTP + alpha-D-glucose 1-phosphate
dGDP-glucose + diphosphate
-
-
-
-
?
dGTP + alpha-D-glucose 1-phosphate
dGDP-glucose + diphosphate
Salmonella enterica typhimurium LT2
-
-
-
-
?
dTDP-glucose + diphosphate
dTTP + alpha-D-glucose 1-phosphate
-
-
-
-
?
dTDP-glucose + diphosphate
dTTP + alpha-D-glucose 1-phosphate
-
protein by ugpG gene is involved in gellan gum biosynthesis and exhibits both dTDP- and UDP-glucose diphosphorylase activities
-
-
?
dTDP-glucose + diphosphate
dTTP + alpha-D-glucose 1-phosphate
-
protein encoded by rmlA gene is involved in gellan gum biosynthesis and exhibits both dTDP- and UDP-glucose diphosphorylase activities
-
-
?
dTTP + alpha-D-galactose 1-phosphate
dTDP-galactose + diphosphate
-
12.6% of the activity with alpha-D-glucose 1-phosphate
-
-
?
dTTP + alpha-D-galactose 1-phosphate
dTDP-galactose + diphosphate
10% activity compared to alpha-D-glucose 1-phosphate
-
-
r
dTTP + alpha-D-galactose 1-phosphate
dTDP-galactose + diphosphate
-
12.6% of the activity with alpha-D-glucose 1-phosphate
-
-
?
dTTP + alpha-D-galactose 1-phosphate
dTDP-galactose + diphosphate
10% activity compared to alpha-D-glucose 1-phosphate
-
-
r
dTTP + alpha-D-glucose 1-phosphate
diphosphate + dTDP-alpha-D-glucose
-
-
-
?
dTTP + alpha-D-glucose 1-phosphate
diphosphate + dTDP-alpha-D-glucose
-
-
-
?
dTTP + alpha-D-glucose 1-phosphate
diphosphate + dTDP-alpha-D-glucose
-
-
-
?
dTTP + alpha-D-glucose 1-phosphate
diphosphate + dTDP-alpha-D-glucose
RML-1 demonstrates a broad substrate range toward five nucleotides (dTTP, UTP, CTP, ATP and GTP) in the presence of alpha-D-glucose 1-phosphate, with higher activity toward dTTP and UTP
-
-
?
dTTP + alpha-D-glucose 1-phosphate
diphosphate + dTDP-alpha-D-glucose
-
-
-
?
dTTP + alpha-D-glucose 1-phosphate
diphosphate + dTDP-alpha-D-glucose
the enzyme is involved in the biosynthesis of the dTDP-3-amino-3,6-dideoxy-D-galactose and dTDP-3-amino-3,6-dideoxy-D-glucose
-
-
?
dTTP + alpha-D-glucose 1-phosphate
diphosphate + dTDP-alpha-D-glucose
-
-
-
?
dTTP + alpha-D-glucose 1-phosphate
diphosphate + dTDP-alpha-D-glucose
-
-
-
?
dTTP + alpha-D-glucose 1-phosphate
diphosphate + dTDP-alpha-D-glucose
-
-
-
-
?
dTTP + alpha-D-glucose 1-phosphate
diphosphate + dTDP-alpha-D-glucose
-
-
-
-
?
dTTP + alpha-D-glucose 1-phosphate
diphosphate + dTDP-alpha-D-glucose
-
-
-
?
dTTP + alpha-D-glucose 1-phosphate
diphosphate + dTDP-alpha-D-glucose
-
86% yield with 0.006 mM enzyme, 93% yield with 0.06 mM enzyme
-
-
?
dTTP + alpha-D-glucose 1-phosphate
diphosphate + dTDP-alpha-D-glucose
-
-
-
?
dTTP + alpha-D-glucose 1-phosphate
diphosphate + dTDP-alpha-D-glucose
-
-
-
r
dTTP + alpha-D-glucose 1-phosphate
diphosphate + dTDP-alpha-D-glucose
maximal velocity of the reverse reaction is 11.4fold of the maximal velocity of the forward reaction. The multifunctional enzyme also shows activity with UTP + alpha-D-glucose 1-phosphate (EC 2.7.7.9, UTP-glucose-1-phosphate uridylyltransferase), UTP + N-acetylglucosamine 1-phosphate (EC 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase), dTTP + N-acetylglucosamine 1-phosphate (N-acetylglucosamine 1-phosphate thymidylyltransferase), dCTP + alpha-D-glucose 1-phosphate (glucose-1-phosphate cytidylyltransferase), dGTP + alpha-D-glucose 1-phosphate (glucose-1-phosphate guanylyltransferase), dATP + alpha-D-glucose 1-phosphate (glucose-1-phosphate adenylyltransferase). No activity with: alpha-D-glucose 1-phosphate + dATP, alpha-D-glucose 1-phosphate + dCTP, alpha-D-glucose 1-phosphate + dGTP, UTP + alpha-D-mannose1-phosphate, UTP + alpha-D-galactose 1-phosphate, UTP + alpha-D-glucosamine 1-phosphate
-
-
r
dTTP + alpha-D-glucose 1-phosphate
diphosphate + dTDP-alpha-D-glucose
the enzyme also shows activity of EC 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase
-
-
?
dTTP + alpha-D-glucose 1-phosphate
diphosphate + dTDP-alpha-D-glucose
the enzyme is involved in biosynthesis of L-rhamnose
-
-
?
dTTP + alpha-D-glucose 1-phosphate
diphosphate + dTDP-alpha-D-glucose
maximal velocity of the reverse reaction is 11.4fold of the maximal velocity of the forward reaction. The multifunctional enzyme also shows activity with UTP + alpha-D-glucose 1-phosphate (EC 2.7.7.9, UTP-glucose-1-phosphate uridylyltransferase), UTP + N-acetylglucosamine 1-phosphate (EC 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase), dTTP + N-acetylglucosamine 1-phosphate (N-acetylglucosamine 1-phosphate thymidylyltransferase), dCTP + alpha-D-glucose 1-phosphate (glucose-1-phosphate cytidylyltransferase), dGTP + alpha-D-glucose 1-phosphate (glucose-1-phosphate guanylyltransferase), dATP + alpha-D-glucose 1-phosphate (glucose-1-phosphate adenylyltransferase). No activity with: alpha-D-glucose 1-phosphate + dATP, alpha-D-glucose 1-phosphate + dCTP, alpha-D-glucose 1-phosphate + dGTP, UTP + alpha-D-mannose1-phosphate, UTP + alpha-D-galactose 1-phosphate, UTP + alpha-D-glucosamine 1-phosphate
-
-
r
dTTP + alpha-D-glucose 1-phosphate
diphosphate + dTDP-alpha-D-glucose
the enzyme also shows activity of EC 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase
-
-
?
dTTP + alpha-D-glucose 1-phosphate
diphosphate + dTDP-alpha-D-glucose
-
-
-
?
dTTP + alpha-D-glucose 1-phosphate
diphosphate + dTDP-alpha-D-glucose
-
-
-
r
dTTP + alpha-D-glucose 1-phosphate
dTDP-alpha-D-glucose + diphosphate
-
-
-
?
dTTP + alpha-D-glucose 1-phosphate
dTDP-alpha-D-glucose + diphosphate
-
-
-
?
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
-
-
-
-
r
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
-
-
-
-
?
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
-
-
-
-
?
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
-
probably involved in conversion of D-glucose to L-rhamnose in the biosynthesis of deoxy compound
-
-
?
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
-
-
-
-
?
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
-
-
-
-
r
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
-
-
-
-
?
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
-
-
-
-
?
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
-
-
-
-
?
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
-
-
-
-
?
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
-
-
-
-
?
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
-
-
-
-
?
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
-
-
-
-
?
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
-
-
-
-
?
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
-
-
-
-
r
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
-
-
-
-
?
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
-
-
-
-
?
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
-
-
-
-
r
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
-
first enzyme of the dTDP-L-rhamnose synthesis pathway
-
-
?
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
-
-
-
-
?
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
-
-
-
-
?
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
Salmonella enterica typhimurium LT2
-
-
-
-
?
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
Streptococcus zymogenes
-
-
-
-
?
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
-
-
-
-
r
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
-
-
-
-
r
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
-
sequential mechanism
-
-
?
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
-
the enzyme catalyzes the first step in the biosynthesis of 4-deoxy-4-(dimethylamino)-5,5-dimethyl-D-ribopyranose moiety
-
-
?
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
-
highly specific for dTDP-glucose and diphosphate
-
r
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
100% activity
-
-
r
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
-
in the opposite direction the enzyme is highly specific for dTDP-D-glucose and diphosphate
-
-
r
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
-
in the opposite direction the enzyme is highly specific for dTDP-D-glucose and diphosphate
-
-
r
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
-
highly specific for dTDP-glucose and diphosphate
-
r
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
100% activity
-
-
r
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
-
-
-
?
GTP + alpha-D-glucose 1-phosphate
diphosphate + GDP-alpha-D-glucose
RML-1 demonstrates a broad substrate range toward five nucleotides (dTTP, UTP, CTP, ATP and GTP) in the presence of alpha-D-glucose 1-phosphate, with higher activity toward dTTP and UTP
-
-
?
GTP + alpha-D-glucose 1-phosphate
diphosphate + GDP-alpha-D-glucose
-
-
-
-
?
GTP + alpha-D-glucose 1-phosphate
diphosphate + GDP-alpha-D-glucose
-
no product yield with 0.006 mM enzyme, 91% yield with 0.06 mM enzyme
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-alpha-D-glucose
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-alpha-D-glucose
RML-1 demonstrates a broad substrate range toward five nucleotides (dTTP, UTP, CTP, ATP and GTP) in the presence of alpha-D-glucose 1-phosphate, with higher activity toward dTTP and UTP
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-alpha-D-glucose
-
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-alpha-D-glucose
-
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-alpha-D-glucose
-
40% yield with 0.006 mM enzyme, 94% yield with 0.06 mM enzyme
-
-
?
UTP + alpha-D-glucose 1-phosphate
UDP-glucose + diphosphate
-
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
UDP-glucose + diphosphate
-
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
UDP-glucose + diphosphate
-
5.5% of the activity with dTTP
-
-
?
UTP + alpha-D-glucose 1-phosphate
UDP-glucose + diphosphate
12% activity compared to dTTP
-
-
r
UTP + alpha-D-glucose 1-phosphate
UDP-glucose + diphosphate
-
5.5% of the activity with dTTP
-
-
?
UTP + alpha-D-glucose 1-phosphate
UDP-glucose + diphosphate
12% activity compared to dTTP
-
-
r
additional information
?
-
-
BtrD is inactive with ATP, GTP, or CTP
-
-
?
additional information
?
-
-
utilizes all eight naturally occurring NTPs with varying levels of catalytic efficiency, even in the presence of nonnative sugar-1-phosphates
-
-
?
additional information
?
-
Salmonella enterica typhimurium LT2
-
utilizes all eight naturally occurring NTPs with varying levels of catalytic efficiency, even in the presence of nonnative sugar-1-phosphates
-
-
?
additional information
?
-
-
no activity with alpha-D-galacturonic acid 1-phosphate
-
-
-
additional information
?
-
-
GTP, UTP/alpha-D-mannose-1-phosphate or UTP/alpha-D-galactose-1-phosphate are no substrates
-
-
?
additional information
?
-
GTP, UTP/alpha-D-mannose-1-phosphate or UTP/alpha-D-galactose-1-phosphate are no substrates
-
-
?
additional information
?
-
-
the enzyme shows 102% relative activity for dTMP or dTDP compared to dTTP at 2 mM, respectively
-
-
?
additional information
?
-
the enzyme shows 102% relative activity for dTMP or dTDP compared to dTTP at 2 mM, respectively
-
-
?
additional information
?
-
-
almost no activity with ATP-D-glucose, CTP-D-glucose, UTP-D-Glucose, GTP, alpha-D-galactose-1-phosphate, and alpha-D-mannose-1-phosphate
-
-
?
additional information
?
-
almost no activity with ATP-D-glucose, CTP-D-glucose, UTP-D-Glucose, GTP, alpha-D-galactose-1-phosphate, and alpha-D-mannose-1-phosphate
-
-
?
additional information
?
-
-
GTP, UTP/alpha-D-mannose-1-phosphate or UTP/alpha-D-galactose-1-phosphate are no substrates
-
-
?
additional information
?
-
GTP, UTP/alpha-D-mannose-1-phosphate or UTP/alpha-D-galactose-1-phosphate are no substrates
-
-
?
additional information
?
-
-
the enzyme shows 102% relative activity for dTMP or dTDP compared to dTTP at 2 mM, respectively
-
-
?
additional information
?
-
the enzyme shows 102% relative activity for dTMP or dTDP compared to dTTP at 2 mM, respectively
-
-
?
additional information
?
-
-
almost no activity with ATP-D-glucose, CTP-D-glucose, UTP-D-Glucose, GTP, alpha-D-galactose-1-phosphate, and alpha-D-mannose-1-phosphate
-
-
?
additional information
?
-
almost no activity with ATP-D-glucose, CTP-D-glucose, UTP-D-Glucose, GTP, alpha-D-galactose-1-phosphate, and alpha-D-mannose-1-phosphate
-
-
?
additional information
?
-
the multifunctional enzyme is involved in biosynthesis of UDP-N-acetyl-alpha-D-glucosamine, an activated and essential form of N-acetyl-alpha-D-glucosamine that is an important component in the polysaccharide structure of most organisms
-
-
?
additional information
?
-
the multifunctional enzyme is involved in biosynthesis of UDP-N-acetyl-alpha-D-glucosamine, an activated and essential form of N-acetyl-alpha-D-glucosamine that is an important component in the polysaccharide structure of most organisms
-
-
?
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Ca2+
-
5.5 mM, 2.1% activity
Ca2+
2 mM. Activation of UDP-N-acetylglucosamine diphosphorylase activity in the order of decreasing effectiveness: Co2+, Mn2+, Mg2+, Zn2+, Ca2+. No activity is detectable without a divalent cation
Ca2+
no activity is detectable when a divalent cation is removed from the reaction buffer. The order of effectiveness of metal ions on GlcNAc-1-P UTase activity is Mg2+ > Zn2+ > Ca2+ > Co2+ > Mn2+, which is different from that of its Glc-1-P TTase activity, Co2+ > Mn2+ > Mg2+ > Zn2+ > Ca2+
Co2+
-
5.5 mM, 124% activity
Co2+
-
absolute requirement for a divalent metal ion. Order of effectiveness is: Mn2+, Mg2+, Zn2+, Cu2+, Fe2+, Ni2+, Co2+
Co2+
absolute requirement for divalent cation. Order of decreasing effectiveness is: Co2+, Mn2+, Mg2+, Zn2+
Co2+
2 mM. Activation of UDP-N-acetylglucosamine diphosphorylase activity in the order of decreasing effectiveness: Co2+, Mn2+, Mg2+, Zn2+, Ca2+. No activity is detectable without a divalent cation
Co2+
absolute requirement for a divalent cation. The order of effectiveness of metal ions on the activity of the enzyme is Co2+, Mn2+, Mg2+ and Zn2+
Co2+
no activity is detectable when a divalent cation is removed from the reaction buffer. The order of effectiveness of metal ions on GlcNAc-1-P UTase activity is Mg2+ > Zn2+ > Ca2+ > Co2+ > Mn2+, which is different from that of its Glc-1-P TTase activity, Co2+ > Mn2+ > Mg2+ > Zn2+ > Ca2+
Cu2+
-
5.5 mM, 0.6% activity
Cu2+
-
absolute requirement for a divalent metal ion. Order of effectiveness is: Mn2+, Mg2+, Zn2+, Cu2+, Fe2+, Ni2+, Co2+
Fe2+
-
5.5 mM, 0.3% activity
Fe2+
-
absolute requirement for a divalent metal ion. Order of effectiveness is: Mn2+, Mg2+, Zn2+, Cu2+, Fe2+, Ni2+, Co2+
Mg2+
absolutely required for catalysis
Mg2+
-
absolute requirement, 2 mM: optimal
Mg2+
-
required, optimal concentration 5 mM
Mg2+
in the RmlA/dTTP complex two Mg2+ are observed binding to the nucleotide
Mg2+
-
5.5 mM, 100% activity
Mg2+
-
absolute requirement, 2 mM: optimal
Mg2+
required for activity, highest activity between 0.63 and 40 mM, 5-10 mM used in assay conditions
Mg2+
-
absolute requirement for a divalent metal ion. Order of effectiveness is: Mn2+, Mg2+, Zn2+, Cu2+, Fe2+, Ni2+, Co2+
Mg2+
absolute requirement for divalent cation. Order of decreasing effectiveness is: Co2+, Mn2+, Mg2+, Zn2+. Optimal Mg2+ concentration is 6 mM
Mg2+
2 mM. Activation of UDP-N-acetylglucosamine diphosphorylase activity in the order of decreasing effectiveness: Co2+, Mn2+, Mg2+, Zn2+, Ca2+. No activity is detectable without a divalent cation
Mg2+
absolute requirement for a divalent cation. The order of effectiveness of metal ions on the activity of the enzyme is Co2+, Mn2+, Mg2+ and Zn2+. The optimal Mg2+ concentration is 6 mM, but the enzymatic activity does not change substantially between 2 and 12 mM
Mg2+
no activity is detectable when a divalent cation is removed from the reaction buffer. The order of effectiveness of metal ions on GlcNAc-1-P UTase activity is Mg2+ > Zn2+ > Ca2+ > Co2+ > Mn2+, which is different from that of its Glc-1-P TTase activity, Co2+ > Mn2+ > Mg2+ > Zn2+ > Ca2+
Mn2+
-
-
Mn2+
-
5.5 mM, 98.5% activity
Mn2+
-
absolute requirement for a divalent metal ion. Order of effectiveness is: Mn2+, Mg2+, Zn2+, Cu2+, Fe2+, Ni2+, Co2+
Mn2+
absolute requirement for divalent cation. Order of decreasing effectiveness is: Co2+, Mn2+, Mg2+, Zn2+
Mn2+
2 mM. Activation of UDP-N-acetylglucosamine diphosphorylase activity in the order of decreasing effectiveness: Co2+, Mn2+, Mg2+, Zn2+, Ca2+. No activity is detectable without a divalent cation
Mn2+
absolute requirement for a divalent cation. The order of effectiveness of metal ions on the activity of the enzyme is Co2+, Mn2+, Mg2+ and Zn2+
Mn2+
no activity is detectable when a divalent cation is removed from the reaction buffer. The order of effectiveness of metal ions on GlcNAc-1-P UTase activity is Mg2+ > Zn2+ > Ca2+ > Co2+ > Mn2+, which is different from that of its Glc-1-P TTase activity, Co2+ > Mn2+ > Mg2+ > Zn2+ > Ca2+
Ni2+
-
5.5 mM, 97.1% activity
Ni2+
-
absolute requirement for a divalent metal ion. Order of effectiveness is: Mn2+, Mg2+, Zn2+, Cu2+, Fe2+, Ni2+, Co2+
Zn2+
-
5.5 mM, 17.9% activity
Zn2+
-
absolute requirement for a divalent metal ion. Order of effectiveness is: Mn2+, Mg2+, Zn2+, Cu2+, Fe2+, Ni2+, Co2+
Zn2+
absolute requirement for divalent cation. Order of decreasing effectiveness is: Co2+, Mn2+, Mg2+, Zn2+
Zn2+
2 mM. Activation of UDP-N-acetylglucosamine diphosphorylase activity in the order of decreasing effectiveness: Co2+, Mn2+, Mg2+, Zn2+, Ca2+. No activity is detectable without a divalent cation
Zn2+
absolute requirement for a divalent cation. The order of effectiveness of metal ions on the activity of the enzyme is Co2+, Mn2+, Mg2+ and Zn2+
Zn2+
no activity is detectable when a divalent cation is removed from the reaction buffer. The order of effectiveness of metal ions on GlcNAc-1-P UTase activity is Mg2+ > Zn2+ > Ca2+ > Co2+ > Mn2+, which is different from that of its Glc-1-P TTase activity, Co2+ > Mn2+ > Mg2+ > Zn2+ > Ca2+
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0.69 - 0.7
2-deoxy-2-amino-alpha-D-glucose 1-phosphate
1.1
3-deoxy-3-amino-alpha-D-glucose 1-phosphate
-
cosubstrate dTTP, pH 7.5, temperature not specified in the publication
1.1
3-deoxy-3-azido-alpha-D-glucose 1-phosphate
-
cosubstrate dTTP, pH 7.5, temperature not specified in the publication
0.03224 - 1.12
alpha-D-glucose 1-phosphate
0.05
dTDP-alpha-D-glucose
0.0094 - 0.095
dTDP-glucose
0.05
TDP-glucose
-
25°C, pH 8
0.18 - 0.38
[[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl]methyl]phosphonic acid
0.69
2-deoxy-2-amino-alpha-D-glucose 1-phosphate
-
cosubstrate dTTP, pH 7.5, temperature not specified in the publication
0.7
2-deoxy-2-amino-alpha-D-glucose 1-phosphate
-
cosubstrate UTP, pH 7.5, temperature not specified in the publication
0.03224
alpha-D-glucose 1-phosphate
cosubstrate: UTP, pH 7.5, 25°C
0.0327
alpha-D-glucose 1-phosphate
at pH 7.5 and 25°C
0.034
alpha-D-glucose 1-phosphate
-
37°C pH 8
0.04988
alpha-D-glucose 1-phosphate
cosubstrate: dTTP, pH 7.5, 25°C
0.069
alpha-D-glucose 1-phosphate
-
pH 7.5, 37°C
0.083
alpha-D-glucose 1-phosphate
-
cosubstrate dTTP, pH 7.5, temperature not specified in the publication
0.16
alpha-D-glucose 1-phosphate
-
cosubstrate UTP, pH 7.5, temperature not specified in the publication
0.226
alpha-D-glucose 1-phosphate
-
cosubstrate UTP, pH 7.6, 37°C
0.8
alpha-D-glucose 1-phosphate
-
37°C pH 8
1.12
alpha-D-glucose 1-phosphate
pH 7.5, 80°C
1.12
alpha-D-glucose 1-phosphate
pH 8.0, 80°C
0.336
ATP
-
pH 7.6, 70°C
3.4
ATP
-
mutant enzyme Q83S, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C
3.4
ATP
-
apparent value, mutant enzyme Q83S, in 100 mM Tris-HCl, pH 8.0, at 37°C
9
ATP
-
wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C
9
ATP
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37°C
0.276
CTP
-
pH 7.6, 70°C
13.7
CTP
-
wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C
13.7
CTP
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37°C
1.1
dATP
-
mutant enzyme Q83S, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C
1.1
dATP
-
apparent value, mutant enzyme Q83S, in 100 mM Tris-HCl, pH 8.0, at 37°C
7.8
dATP
-
wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C
7.8
dATP
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37°C
10.5
dCTP
-
wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C
10.5
dCTP
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37°C
3.7
dGTP
-
wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C
3.7
dGTP
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37°C
11.5
dGTP
-
mutant enzyme Q83D, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C
11.5
dGTP
-
apparent value, mutant enzyme Q83D, in 100 mM Tris-HCl, pH 8.0, at 37°C
0.154
diphosphate
-
37°C pH 8
0.39
diphosphate
pH 7.5, 80°C
0.39
diphosphate
pH 8.0, 80°C
0.05
dTDP-alpha-D-glucose
pH 7.5, 80°C
0.05
dTDP-alpha-D-glucose
pH 8.0, 80°C
0.0094
dTDP-glucose
-
UgpG protein
0.012
dTDP-glucose
-
RmlA protein
0.095
dTDP-glucose
-
37°C pH 8
0.0106
dTTP
at pH 7.5 and 25°C
0.02422
dTTP
pH 7.5, 25°C
0.29
dTTP
-
wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C
0.29
dTTP
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37°C
0.366
dTTP
-
pH 7.6, 70°C
1.66
dTTP
pH 7.2, 37°C, fusion protein of isoform DnmL with C-terminal sequence of isoform RmbA
1.72
dTTP
pH 7.2, 37°, mutant R313S/T317V/H322A/A339S/S341H/D348H
2.4
dTTP
-
mutant enzyme Q83S, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C
2.4
dTTP
-
apparent value, mutant enzyme Q83S, in 100 mM Tris-HCl, pH 8.0, at 37°C
6.2
dTTP
-
mutant enzyme Q83D, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C
6.2
dTTP
-
apparent value, mutant enzyme Q83D, in 100 mM Tris-HCl, pH 8.0, at 37°C
0.184
GTP
-
pH 7.6, 37°C
1.3
GTP
-
mutant enzyme Q83D, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C
1.3
GTP
-
apparent value, mutant enzyme Q83D, in 100 mM Tris-HCl, pH 8.0, at 37°C
8.5
GTP
-
wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C
8.5
GTP
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37°C
0.00947
UTP
pH 7.5, 25°C
1.2
UTP
-
wild type enzyme
4.6
UTP
-
wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C
4.6
UTP
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37°C
0.18
[[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl]methyl]phosphonic acid
-
cosubstrate dTTP, pH 7.5, temperature not specified in the publication
0.38
[[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl]methyl]phosphonic acid
-
cosubstrate UTP, pH 7.5, temperature not specified in the publication
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0.1 - 4.5
2-deoxy-2-amino-alpha-D-glucose 1-phosphate
0.08
3-deoxy-3-amino-alpha-D-glucose 1-phosphate
-
cosubstrate dTTP, pH 7.5, temperature not specified in the publication
0.0003
3-deoxy-3-azido-alpha-D-glucose 1-phosphate
-
cosubstrate dTTP, pH 7.5, temperature not specified in the publication
0.016 - 24
alpha-D-glucose 1-phosphate
0.01 - 0.55
[[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl]methyl]phosphonic acid
0.1
2-deoxy-2-amino-alpha-D-glucose 1-phosphate
-
cosubstrate UTP, pH 7.5, temperature not specified in the publication
4.5
2-deoxy-2-amino-alpha-D-glucose 1-phosphate
-
cosubstrate dTTP, pH 7.5, temperature not specified in the publication
0.016
alpha-D-glucose 1-phosphate
-
cosubstrate UTP, pH 7.5, temperature not specified in the publication
6.18
alpha-D-glucose 1-phosphate
-
cosubstrate UTP, pH 7.6, 37°C
7.3
alpha-D-glucose 1-phosphate
at pH 7.5 and 25°C
23.24
alpha-D-glucose 1-phosphate
cosubstrate: UTP, pH 7.5, 25°C
23.58
alpha-D-glucose 1-phosphate
cosubstrate: dTTP, pH 7.5, 25°C
24
alpha-D-glucose 1-phosphate
-
cosubstrate dTTP, pH 7.5, temperature not specified in the publication
0.06
ATP
-
wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C
0.06
ATP
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37°C
0.48
ATP
-
mutant enzyme Q83S, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C
0.48
ATP
-
apparent value, mutant enzyme Q83S, in 100 mM Tris-HCl, pH 8.0, at 37°C
0.098
CTP
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37°C
0.1
CTP
-
wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C
1.88
dATP
-
wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C
1.88
dATP
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37°C
5.33
dATP
-
mutant enzyme Q83S, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C
5.33
dATP
-
apparent value, mutant enzyme Q83S, in 100 mM Tris-HCl, pH 8.0, at 37°C
1.22
dCTP
-
wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C
1.22
dCTP
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37°C
2.12
dGTP
-
wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C
2.12
dGTP
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37°C
5
dGTP
-
mutant enzyme Q83D, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C
5
dGTP
-
apparent value, mutant enzyme Q83D, in 100 mM Tris-HCl, pH 8.0, at 37°C
0.56
dTDP-glucose
-
UgpG protein
1.35
dTDP-glucose
-
RmlA protein
0.37
dTTP
-
pH 7.6, 37°C
3.13
dTTP
-
mutant enzyme Q83D, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C
3.62
dTTP
-
apparent value, mutant enzyme Q83D, in 100 mM Tris-HCl, pH 8.0, at 37°C
6.33
dTTP
-
mutant enzyme Q83S, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C
6.33
dTTP
-
apparent value, mutant enzyme Q83S, in 100 mM Tris-HCl, pH 8.0, at 37°C
7.9
dTTP
at pH 7.5 and 25°C
15
dTTP
-
wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C
30.83
dTTP
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37°C
0.14
GTP
-
wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C
0.14
GTP
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37°C
0.21
GTP
-
mutant enzyme Q83D, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C
0.21
GTP
-
apparent value, mutant enzyme Q83D, in 100 mM Tris-HCl, pH 8.0, at 37°C
2.1
UTP
-
pH 7.6, 37°C
24
UTP
-
wild type enzyme
41
UTP
-
wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C
41
UTP
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37°C
0.01
[[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl]methyl]phosphonic acid
-
cosubstrate UTP, pH 7.5, temperature not specified in the publication
0.55
[[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl]methyl]phosphonic acid
-
cosubstrate dTTP, pH 7.5, temperature not specified in the publication
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Pazur, J.H.; Shuey, E.W.
The enzymatic synthesis of thymidine diphosphate glucose and its conversion to thymidine diphosphate rhamnose
J. Biol. Chem.
236
1780-1785
1961
Bacillus subtilis, Leuconostoc mesenteroides, Escherichia coli, Enterococcus faecalis, Glycine max, Lactiplantibacillus plantarum, Medicago sativa, Micrococcus luteus, Streptococcus zymogenes, Leuconostoc mesenteroides P-60
brenda
Kornfeld, S.; Glaser, L.
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