Information on EC 2.7.7.24 - glucose-1-phosphate thymidylyltransferase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

EC NUMBER
COMMENTARY hide
2.7.7.24
-
RECOMMENDED NAME
GeneOntology No.
glucose-1-phosphate thymidylyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
dTTP + alpha-D-glucose 1-phosphate = diphosphate + dTDP-alpha-D-glucose
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nucleotidyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of antibiotics
-
-
dTDP-3-acetamido-3,6-dideoxy-alpha-D-glucose biosynthesis
-
-
dTDP-3-acetamido-alpha-D-fucos biosynthesis
-
-
dTDP-4-O-demethyl-beta-L-noviose biosynthesis
-
-
dTDP-6-deoxy-alpha-D-allose biosynthesis
-
-
dTDP-alpha-D-mycaminose biosynthesis
-
-
dTDP-beta-L-4-epi-vancosamine biosynthesis
-
-
dTDP-beta-L-digitoxose biosynthesis
-
-
dTDP-D-beta-fucofuranose biosynthesis
-
-
dTDP-D-desosamine biosynthesis
-
-
dTDP-D-forosamine biosynthesis
-
-
dTDP-D-olivose, dTDP-D-oliose and dTDP-D-mycarose biosynthesis
-
-
dTDP-D-ravidosamine and dTDP-4-acetyl-D-ravidosamine biosynthesis
-
-
dTDP-L-megosamine biosynthesis
-
-
dTDP-L-mycarose biosynthesis
-
-
dTDP-L-olivose biosynthesis
-
-
dTDP-L-rhamnose biosynthesis I
-
-
dTDP-L-rhamnose biosynthesis II
-
-
dTDP-N-acetylthomosamine biosynthesis
-
-
dTDP-N-acetylviosamine biosynthesis
-
-
dTDPLrhamnose biosynthesis
-
-
Metabolic pathways
-
-
Polyketide sugar unit biosynthesis
-
-
Streptomycin biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
dTTP:alpha-D-glucose-1-phosphate thymidylyltransferase
Involved in the biosynthesis of L-rhamnose in bacteria.
CAS REGISTRY NUMBER
COMMENTARY hide
9026-03-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
ATCC 6633
-
-
Manually annotated by BRENDA team
germinated
-
-
Manually annotated by BRENDA team
ATCC 17-5
-
-
Manually annotated by BRENDA team
strain P-60
-
-
Manually annotated by BRENDA team
strain P-60
-
-
Manually annotated by BRENDA team
germinated
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain mc2155
-
-
Manually annotated by BRENDA team
Salmonella enterica typhimurium
strain LT2
-
-
Manually annotated by BRENDA team
Salmonella enterica typhimurium LT2
strain LT2
-
-
Manually annotated by BRENDA team
ATCC 31461
-
-
Manually annotated by BRENDA team
Streptococcus zymogenes
ATCC 10100
-
-
Manually annotated by BRENDA team
T99
-
-
Manually annotated by BRENDA team
recombinant
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
GERI-155
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
btrD mutant shows no production of any antibiotics under the standard fermentation conditions, while the complementation of this disruption by transformation recovers the antibiotic production
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + alpha-D-glucose 1-phosphate
ADP-glucose + diphosphate
show the reaction diagram
ATP + alpha-D-glucose-1-phosphate
ADP-glucose + diphosphate
show the reaction diagram
CTP + alpha-D-glucose 1-phosphate
CDP-glucose + diphosphate
show the reaction diagram
CTP + alpha-D-glucose-1-phosphate
CDP-glucose + diphosphate
show the reaction diagram
D-glucosamine 1-phosphate + TTP
TTP-D-glucosamine + diphosphate
show the reaction diagram
-
-
-
-
?
D-glucosamine 1-phosphate + UTP
UDP-D-glucosamine + diphosphate
show the reaction diagram
-
-
-
-
?
D-glucose 1-phosphate + UTP
UDP-D-glucose + diphosphate
show the reaction diagram
-
-
-
-
?
dATP + alpha-D-glucose 1-phosphate
dADP-glucose + diphosphate
show the reaction diagram
dCTP + alpha-D-glucose 1-phosphate
dCDP-glucose + diphosphate
show the reaction diagram
dGTP + alpha-D-glucose 1-phosphate
dGDP-glucose + diphosphate
show the reaction diagram
dTDP-glucose + diphosphate
dTTP + alpha-D-glucose 1-phosphate
show the reaction diagram
dTTP + alpha-D-galactose 1-phosphate
dTDP-galactose + diphosphate
show the reaction diagram
dTTP + alpha-D-galactose-1-phosphate
dTDP-galactose + diphosphate
show the reaction diagram
10% of the activity with dTTP and alpha-D-glucose 1-phosphate
-
-
?
dTTP + alpha-D-glucosamine 1-phosphate
dTDP-glucosamine + diphosphate
show the reaction diagram
23% activity compared to alpha-D-glucose 1-phosphate
-
-
r
dTTP + alpha-D-glucosamine-1-phosphate
dTDP-glucosamine + diphosphate
show the reaction diagram
23% of the activity with dTTP and alpha-D-glucose 1-phosphate
-
-
?
dTTP + alpha-D-glucose 1-phosphate
diphosphate + dTDP-alpha-D-glucose
show the reaction diagram
dTTP + alpha-D-glucose 1-phosphate
diphosphate + dTDP-glucose
show the reaction diagram
-
-
-
-
?
dTTP + alpha-D-glucose 1-phosphate
dTDP-alpha-D-glucose + diphosphate
show the reaction diagram
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
dTTP + alpha-D-mannose 1-phosphate
dTDP-mannose + diphosphate
show the reaction diagram
11% activity compared to alpha-D-glucose 1-phosphate
-
-
r
dTTP + alpha-D-mannose-1-phosphate
dTDP-mannose + diphosphate
show the reaction diagram
11% of the activity with dTTP and alpha-D-glucose 1-phosphate
-
-
?
GTP + alpha-D-glucose 1-phosphate
GDP-glucose + diphosphate
show the reaction diagram
Salmonella enterica typhimurium
-
-
-
-
?
TTP + alpha-D-glucose 1-phosphate
TDP-glucose + diphosphate
show the reaction diagram
-
-
-
-
r
UTP + alpha-D-glucosamine 1-phosphate
UDP-glucosamine + diphosphate
show the reaction diagram
8% activity compared to the reaction with alpha-D-glucose 1-phosphate and dTTP
-
-
r
UTP + alpha-D-glucosamine-1-phosphate
UDP-glucosamine + diphosphate
show the reaction diagram
8% of the activity with dTTP and alpha-D-glucose 1-phosphate
-
-
?
UTP + alpha-D-glucose 1-phosphate
UDP-glucose + diphosphate
show the reaction diagram
UTP + alpha-D-glucose-1-phosphate
UDP-glucose + diphosphate
show the reaction diagram
12% of the activity with dTTP and alpha-D-glucose 1-phosphate
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dTDP-glucose + diphosphate
dTTP + alpha-D-glucose 1-phosphate
show the reaction diagram
dTTP + alpha-D-glucose 1-phosphate
dTDP-glucose + diphosphate
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
dTDP-glucose
-
competitive to dTTP
dTDP-L-rhamnose
-
-
dTTP
-
competitive to dTDPglucose and non-competitive to alpha-D-glucose 1-phosphate
N-(6-amino-1-benzyl-1,2,3,4-tetrahydropyrimidin-5-yl)-N-methylbenzenesulfonamide
inhibitor does not bind at the active site of RmlA but binds at a second site remote from the active site. Despite this, the compound acts as competitive inhibitor but with high cooperativity
N-(6-amino-1-benzyl-1,2,3,4-tetrahydropyrimidin-5-yl)-N-methylbutane-1-sulfonamide
inhibitor does not bind at the active site of RmlA but binds at a second site remote from the active site. Despite this, the compound acts as competitive inhibitor but with high cooperativity
N-(6-amino-1-benzyl-1,2,3,4-tetrahydropyrimidin-5-yl)-N-methylfuran-2-sulfonamide
inhibitor does not bind at the active site of RmlA but binds at a second site remote from the active site. Despite this, the compound acts as competitive inhibitor but with high cooperativity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.034 - 1.12
alpha-D-glucose 1-phosphate
3.4 - 9
ATP
13.7
CTP
Salmonella enterica typhimurium
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37C; wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37C
1.1 - 7.8
dATP
10.5
dCTP
Salmonella enterica typhimurium
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37C; wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37C
3.7 - 11.5
dGTP
0.154 - 0.39
diphosphate
0.05
dTDP-alpha-D-glucose
-
pH 7.5, 80C
0.0094 - 0.095
dTDP-glucose
0.02 - 6.2
dTTP
1.3 - 8.5
GTP
0.05
TDP-glucose
-
25C, pH 8
0.1
TTP
-
25C, pH 8
1.2 - 4.6
UTP
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.06 - 0.48
ATP
0.098 - 0.1
CTP
1.88 - 5.33
dATP
1.22
dCTP
Salmonella enterica typhimurium
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37C; wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37C
2.12 - 5
dGTP
0.56 - 1.35
dTDP-glucose
2.9 - 30.83
dTTP
0.14 - 0.21
GTP
24 - 41
UTP
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.1 - 1.15
dTTP
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000073
N-(6-amino-1-benzyl-1,2,3,4-tetrahydropyrimidin-5-yl)-N-methylbenzenesulfonamide
Pseudomonas aeruginosa
Q9HU22
pH not specified in the publication, temperature not specified in the publication
0.000175
N-(6-amino-1-benzyl-1,2,3,4-tetrahydropyrimidin-5-yl)-N-methylbutane-1-sulfonamide
Pseudomonas aeruginosa
Q9HU22
pH not specified in the publication, temperature not specified in the publication
0.000105
N-(6-amino-1-benzyl-1,2,3,4-tetrahydropyrimidin-5-yl)-N-methylfuran-2-sulfonamide
Pseudomonas aeruginosa
Q9HU22
pH not specified in the publication, temperature not specified in the publication
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00085
crude extract, at pH 8.0 and 37C
0.00166
-
-
0.004
after 4.7fold purification, at pH 8.0 and 37C
0.017
crude cell extract
0.0801
after 4.7fold purification
1.35
-
pH 7.5, 80C, substrates: dTTP + alpha-D-glucose 1-phosphate
9.4
-
pH 7.5, 80C, substrates: diphosphate + dTDP-alpha-D-glucose
44.8
-
dTDPglucose + diphosphate
194
-
forward reaction
360
-
reverse reaction
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 8.5
-
-
8 - 8.5
-
broad
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 10
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
34 - 37
-
-
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70 - 100
-
70C: about 40% of maximal activity, 100C: about 80% of maximal activity
PDB
SCOP
CATH
ORGANISM
UNIPROT
Burkholderia vietnamiensis (strain G4 / LMG 22486)
Burkholderia vietnamiensis (strain G4 / LMG 22486)
Burkholderia vietnamiensis (strain G4 / LMG 22486)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Salmonella choleraesuis
Salmonella choleraesuis
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Sulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
Sulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
31360
-
calculated from amino acid sequence
31810
-
mass spectroscopy
33770
-
calculated from nucleic acid sequence
33800
-
MALDI mass spectrum
43000
-
gel filtration
48000
-
gel filtration
56000
SDS-PAGE; SDS-PAGE
106000 - 122000
-
dynamic light scattering
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
tetramer
additional information
-
trimer or tetramer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
co-crystallization with dTDP-glucose or dTMP and alpha-D-glucose 1-phosphate or deoxythymidine and alpha-D-glucose 1-phosphate, hanging-drop vapour-diffusion method in 1.7 M ammonium sulfate and 0.1 M sodium acetate, pH 5.3
-
sitting-drop vapour-diffusion method in polyethylene glycol 6000, 0.1 M sodium citrate, pH 4.6 and 0.5 M Li2SO4 as precipitant, crystallization as apoenzyme or co-crystallization with dTTP or thymidine and alpha-D-glucose 1-phosphate or dTDP-D-glucose or dTDP-L-rhamnose
-
sitting-drop vapour-diffusion method in polyethylene glycol 6000, 0.1 M sodium citrate, pH 4.6 and 0.5 M Li2SO4 as precipitant, space group: P1, the asymmetric unit contains eight monomers in the form of two RmlA tetramers with a solvent content of 51%; sitting-drop vapour-diffusion technique with PEG 6000 and lithium sulfate as precipitant. Several diffraction data sets of single frozen crystals are collected to a resolution of 1.66 A. Crystals belong to space group P1, with unit-cell parameters a = 71.5 A, b = 73.1 A, c = 134.7 A, alpha = 89.9, beta = 80.9, gamma = 81
-
homology modeling of both isoforms DnmL and RmbA
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70
thermostable up to
80
-
half-life: 180 min
95
-
half-life: 60 min
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C at concentrations higher than 5 mg/ml stable for up to three months
-
-80 C, 50 mM potassium phosphate buffer, pH 7.0, containing 1 mM DTT and 20% glycerol, purified SgcA1 is stable for several months with minimal loss of activity
-
stable for several weeks at any step of purification if kept frozen
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
; recombinant enzyme with his-tag
-
ammonium sulfate precipitation and Ni-NTA affinity column chromatography; ammonium sulfate precipitation and Ni-NTA affinity column chromatography
ATCC 10100, partial
Streptococcus zymogenes
-
ATCC 13027, partial
-
ATCC 17-5, partial
-
ATCC 6633, partial
-
ATCC 7700, partial
-
germinated, partial
Ni-NTA agarose resin chromatography; PD-10 gel filtration, nickel-nitrilotriacetic acid agarose resin or spin column chromatography
Salmonella enterica typhimurium
-
partial
recombinant enzyme
-
recombinant enzyme, RmlA protein; recombinant enzyme, UgpG protein
-
strain P-60, partial
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Escherichia coli BL21(DE3)/pNOS110 produces protein mostly in an insoluble form under normal culture conditions
expressed in Escherichia coli
expressed in Escherichia coli BL21 (DE3) cells; expressed in Escherichia coli BL21(DE3) cells
Salmonella enterica typhimurium
-
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli vector pSCHS6; expressed in Escherichia coli, vector pSCHS6
expression in Escherichia coli
expression in Escherichia coli as GST-fusion protein
-
expression in Escherichia coli, protein forms inclusion bodies
expression in Escherichia coli, RmlA protein; expression in Escherichia coli, UgpG protein
-
expression in Escherichia coli, wild-type and truncated enzyme form lacking the 170-residues C-terminal domain
-
overexpression in Escherichia coli
overexpression in Escherichia coli BL21 with a 6 x His-tag
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Q83A
Salmonella enterica typhimurium
-
0.2% relative activity compared to the wild type enzyme, using dTTP as a substrate; reduced activity with dTTP and increased activity with dATP and ATP compared to the wild type enzyme
Q83D
Salmonella enterica typhimurium
-
0.2% relative activity compared to the wild type enzyme, using dTTP as a substrate; reduced activity with dTTP and increased activity with dGTP and GTP compared to the wild type enzyme
Q83E
Salmonella enterica typhimurium
-
1.0% relative activity compared to the wild type enzyme, using dTTP as a substrate; wild type activity with dTTP
Q83N
Salmonella enterica typhimurium
-
0.3% relative activity compared to the wild type enzyme, using dTTP as a substrate; reduced activity with dTTP and increased activity with dATP and ATP compared to the wild type enzyme
Q83S
Salmonella enterica typhimurium
-
0.3% relative activity compared to the wild type enzyme, using dTTP as a substrate; reduced activity with dTTP and increased activity with dATP and ATP compared to the wild type enzyme
Q83D
Salmonella enterica typhimurium LT2
-
0.2% relative activity compared to the wild type enzyme, using dTTP as a substrate; reduced activity with dTTP and increased activity with dGTP and GTP compared to the wild type enzyme
-
Q83N
Salmonella enterica typhimurium LT2
-
0.3% relative activity compared to the wild type enzyme, using dTTP as a substrate
-
Q83S
Salmonella enterica typhimurium LT2
-
0.3% relative activity compared to the wild type enzyme, using dTTP as a substrate; reduced activity with dTTP and increased activity with dATP and ATP compared to the wild type enzyme
-
R313S/T317V/H322A/A339S/S341H/D348H
D208A
-
UDP-N-acetylglucosamine diphosphorylase activity is very low
E146A
-
UDP-N-acetylglucosamine diphosphorylase activity is very low
G9A
-
UDP-N-acetylglucosamine diphosphorylase activity is very low
K147A
-
UDP-N-acetylglucosamine diphosphorylase activity is very low
R13A
-
UDP-N-acetylglucosamine diphosphorylase activity is very low
T80A
-
enhanced UDP-N-acetylglucosamine diphosphorylase activity under optimal conditions
Y97A
-
enhanced UDP-N-acetylglucosamine diphosphorylase activity under optimal conditions
Y97F
-
UDP-N-acetylglucosamine diphosphorylase activity is very low
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
-
microtiter platebased colorimetric assay for RmlA enzyme activity, based on hydrolysis of the diphosphate formed and detection of phosphate by malachite green reagent
medicine
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