Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) cells | Salmonella enterica typhimurium |
expressed in Escherichia coli BL21(DE3) cells | Salmonella enterica typhimurium |
Protein Variants | Comment | Organism |
---|---|---|
Q83A | 0.2% relative activity compared to the wild type enzyme, using dTTP as a substrate | Salmonella enterica typhimurium |
Q83A | reduced activity with dTTP and increased activity with dATP and ATP compared to the wild type enzyme | Salmonella enterica typhimurium |
Q83D | 0.2% relative activity compared to the wild type enzyme, using dTTP as a substrate | Salmonella enterica typhimurium |
Q83D | reduced activity with dTTP and increased activity with dGTP and GTP compared to the wild type enzyme | Salmonella enterica typhimurium |
Q83E | 1.0% relative activity compared to the wild type enzyme, using dTTP as a substrate | Salmonella enterica typhimurium |
Q83E | wild type activity with dTTP | Salmonella enterica typhimurium |
Q83N | 0.3% relative activity compared to the wild type enzyme, using dTTP as a substrate | Salmonella enterica typhimurium |
Q83N | reduced activity with dTTP and increased activity with dATP and ATP compared to the wild type enzyme | Salmonella enterica typhimurium |
Q83S | 0.3% relative activity compared to the wild type enzyme, using dTTP as a substrate | Salmonella enterica typhimurium |
Q83S | reduced activity with dTTP and increased activity with dATP and ATP compared to the wild type enzyme | Salmonella enterica typhimurium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.29 | - |
dTTP | wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
0.29 | - |
dTTP | apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
1.1 | - |
dATP | mutant enzyme Q83S, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
1.1 | - |
dATP | apparent value, mutant enzyme Q83S, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
1.3 | - |
GTP | mutant enzyme Q83D, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
1.3 | - |
GTP | apparent value, mutant enzyme Q83D, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
2.4 | - |
dTTP | mutant enzyme Q83S, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
2.4 | - |
dTTP | apparent value, mutant enzyme Q83S, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
3.4 | - |
ATP | mutant enzyme Q83S, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
3.4 | - |
ATP | apparent value, mutant enzyme Q83S, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
3.7 | - |
dGTP | wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
3.7 | - |
dGTP | apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
4.6 | - |
UTP | wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
4.6 | - |
UTP | apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
6.2 | - |
dTTP | mutant enzyme Q83D, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
6.2 | - |
dTTP | apparent value, mutant enzyme Q83D, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
7.8 | - |
dATP | wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
7.8 | - |
dATP | apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
8.5 | - |
GTP | wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
8.5 | - |
GTP | apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
9 | - |
ATP | wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
9 | - |
ATP | apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
10.5 | - |
dCTP | wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
10.5 | - |
dCTP | apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
11.5 | - |
dGTP | mutant enzyme Q83D, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
11.5 | - |
dGTP | apparent value, mutant enzyme Q83D, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
13.7 | - |
CTP | wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
13.7 | - |
CTP | apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica typhimurium | - |
- |
- |
Salmonella enterica typhimurium LT2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose resin chromatography | Salmonella enterica typhimurium |
PD-10 gel filtration, nickel-nitrilotriacetic acid agarose resin or spin column chromatography | Salmonella enterica typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-D-glucose 1-phosphate | - |
Salmonella enterica typhimurium | ADP-glucose + diphosphate | - |
? | |
CTP + alpha-D-glucose 1-phosphate | - |
Salmonella enterica typhimurium | CDP-glucose + diphosphate | - |
? | |
dATP + alpha-D-glucose 1-phosphate | - |
Salmonella enterica typhimurium | dADP-glucose + diphosphate | - |
? | |
dATP + alpha-D-glucose 1-phosphate | - |
Salmonella enterica typhimurium LT2 | dADP-glucose + diphosphate | - |
? | |
dCTP + alpha-D-glucose 1-phosphate | - |
Salmonella enterica typhimurium | dCDP-glucose + diphosphate | - |
? | |
dCTP + alpha-D-glucose 1-phosphate | - |
Salmonella enterica typhimurium LT2 | dCDP-glucose + diphosphate | - |
? | |
dGTP + alpha-D-glucose 1-phosphate | - |
Salmonella enterica typhimurium | dGDP-glucose + diphosphate | - |
? | |
dGTP + alpha-D-glucose 1-phosphate | - |
Salmonella enterica typhimurium LT2 | dGDP-glucose + diphosphate | - |
? | |
dTTP + alpha-D-glucose 1-phosphate | - |
Salmonella enterica typhimurium | dTDP-glucose + diphosphate | - |
? | |
dTTP + alpha-D-glucose 1-phosphate | - |
Salmonella enterica typhimurium LT2 | dTDP-glucose + diphosphate | - |
? | |
GTP + alpha-D-glucose 1-phosphate | - |
Salmonella enterica typhimurium | GDP-glucose + diphosphate | - |
? | |
additional information | utilizes all eight naturally occurring NTPs with varying levels of catalytic efficiency, even in the presence of nonnative sugar-1-phosphates | Salmonella enterica typhimurium | ? | - |
? | |
additional information | utilizes all eight naturally occurring NTPs with varying levels of catalytic efficiency, even in the presence of nonnative sugar-1-phosphates | Salmonella enterica typhimurium LT2 | ? | - |
? | |
UTP + alpha-D-glucose 1-phosphate | - |
Salmonella enterica typhimurium | UDP-glucose + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RmlA | - |
Salmonella enterica typhimurium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
ATP | wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
0.06 | - |
ATP | apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
0.098 | - |
CTP | apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
0.1 | - |
CTP | wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
0.14 | - |
GTP | wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
0.14 | - |
GTP | apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
0.21 | - |
GTP | mutant enzyme Q83D, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
0.21 | - |
GTP | apparent value, mutant enzyme Q83D, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
0.48 | - |
ATP | mutant enzyme Q83S, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
0.48 | - |
ATP | apparent value, mutant enzyme Q83S, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
1.22 | - |
dCTP | wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
1.22 | - |
dCTP | apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
1.88 | - |
dATP | wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
1.88 | - |
dATP | apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
2.12 | - |
dGTP | wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
2.12 | - |
dGTP | apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
3.13 | - |
dTTP | mutant enzyme Q83D, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
3.62 | - |
dTTP | apparent value, mutant enzyme Q83D, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
5 | - |
dGTP | mutant enzyme Q83D, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
5 | - |
dGTP | apparent value, mutant enzyme Q83D, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
5.33 | - |
dATP | mutant enzyme Q83S, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
5.33 | - |
dATP | apparent value, mutant enzyme Q83S, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
6.33 | - |
dTTP | mutant enzyme Q83S, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
6.33 | - |
dTTP | apparent value, mutant enzyme Q83S, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
15 | - |
dTTP | wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
30.83 | - |
dTTP | apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
41 | - |
UTP | wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium | |
41 | - |
UTP | apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37°C | Salmonella enterica typhimurium |