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Literature summary for 2.7.7.24 extracted from

  • Murrell, J.M.; Liu, W.; Shen, B.
    Biochemical characterization of the SgcA1 alpha-D-glucopyranosyl-1-phosphate thymidylyltransferase from the enediyne antitumor antibiotic C-1027 biosynthetic pathway and overexpression of sgcA1 in Streptomyces globisporus to improve C-1027 production (2004), J. Nat. Prod., 67, 206-213.
    View publication on PubMed
Search for more literature for 2.7.7.24

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Streptomyces globisporus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 dTTP
-
 Streptomyces globisporus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ absolute requirement for a divalent metal ion. Order of effectiveness is: Mn2+, Mg2+, Zn2+, Cu2+, Fe2+, Ni2+, Co2+ Streptomyces globisporus
Cu2+ absolute requirement for a divalent metal ion. Order of effectiveness is: Mn2+, Mg2+, Zn2+, Cu2+, Fe2+, Ni2+, Co2+ Streptomyces globisporus
Fe2+ absolute requirement for a divalent metal ion. Order of effectiveness is: Mn2+, Mg2+, Zn2+, Cu2+, Fe2+, Ni2+, Co2+ Streptomyces globisporus
Mg2+ absolute requirement for a divalent metal ion. Order of effectiveness is: Mn2+, Mg2+, Zn2+, Cu2+, Fe2+, Ni2+, Co2+ Streptomyces globisporus
Mn2+ absolute requirement for a divalent metal ion. Order of effectiveness is: Mn2+, Mg2+, Zn2+, Cu2+, Fe2+, Ni2+, Co2+ Streptomyces globisporus
Ni2+ absolute requirement for a divalent metal ion. Order of effectiveness is: Mn2+, Mg2+, Zn2+, Cu2+, Fe2+, Ni2+, Co2+ Streptomyces globisporus
Zn2+ absolute requirement for a divalent metal ion. Order of effectiveness is: Mn2+, Mg2+, Zn2+, Cu2+, Fe2+, Ni2+, Co2+ Streptomyces globisporus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 gel filtration Streptomyces globisporus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dTTP + alpha-D-glucose 1-phosphate Streptomyces globisporus the enzyme catalyzes the first step in the biosynthesis of 4-deoxy-4-(dimethylamino)-5,5-dimethyl-D-ribopyranose moiety dTDP-glucose + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces globisporus
-
 recombinant
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces globisporus

Storage Stability

Storage Stability Organism
-80 °C, 50 mM potassium phosphate buffer, pH 7.0, containing 1 mM DTT and 20% glycerol, purified SgcA1 is stable for several months with minimal loss of activity Streptomyces globisporus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dTTP + alpha-D-glucose 1-phosphate sequential mechanism Streptomyces globisporus dTDP-glucose + diphosphate
-
 ?
dTTP + alpha-D-glucose 1-phosphate the enzyme catalyzes the first step in the biosynthesis of 4-deoxy-4-(dimethylamino)-5,5-dimethyl-D-ribopyranose moiety Streptomyces globisporus dTDP-glucose + diphosphate
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Streptomyces globisporus

Synonyms

Synonyms Comment Organism
Glc-1-P-TT
-
 Streptomyces globisporus
SgcA1
-
 Streptomyces globisporus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.9
-
 dTTP
-
 Streptomyces globisporus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 9
-
 Streptomyces globisporus