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Literature summary for 2.7.7.24 extracted from

  • Sha, S.; Zhou, Y.; Xin, Y.; Ma, Y.
    Development of a colorimetric assay and kinetic analysis for Mycobacterium tuberculosis D-glucose-1-phosphate thymidylyltransferase (2012), J. Biomol. Screen., 17, 252-257.
    View publication on PubMed

Application

Application Comment Organism
analysis microtiter plate–based colorimetric assay for RmlA enzyme activity, based on hydrolysis of the diphosphate formed and detection of phosphate by malachite green reagent Mycobacterium tuberculosis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
dTTP pH 7.5, 37°C Mycobacterium tuberculosis
0.069
-
alpha-D-glucose 1-phosphate pH 7.5, 37°C Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, optimal concentration 5 mM Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33670
-
x * 33700, SDS-PAGE, x * 33670, calculated Mycobacterium tuberculosis
33700
-
x * 33700, SDS-PAGE, x * 33670, calculated Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dTTP + alpha-D-glucose 1-phosphate
-
Mycobacterium tuberculosis diphosphate + dTDP-glucose
-
?

Subunits

Subunits Comment Organism
? x * 33700, SDS-PAGE, x * 33670, calculated Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
RmlA
-
Mycobacterium tuberculosis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37
-
-
Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Mycobacterium tuberculosis