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Literature summary for 2.7.7.24 extracted from

  • Zhang, Z.; Akutsu, J.; Tsujimura, M.; Kawarabayasi, Y.
    Increasing in archaeal GlcNAc-1-P uridyltransferase activity by targeted mutagenesis while retaining its extreme thermostability (2007), J. Biochem., 141, 553-562.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
D208A UDP-N-acetylglucosamine diphosphorylase activity is very low Sulfurisphaera tokodaii
E146A UDP-N-acetylglucosamine diphosphorylase activity is very low Sulfurisphaera tokodaii
G9A UDP-N-acetylglucosamine diphosphorylase activity is very low Sulfurisphaera tokodaii
K147A UDP-N-acetylglucosamine diphosphorylase activity is very low Sulfurisphaera tokodaii
R13A UDP-N-acetylglucosamine diphosphorylase activity is very low Sulfurisphaera tokodaii
T80A enhanced UDP-N-acetylglucosamine diphosphorylase activity under optimal conditions Sulfurisphaera tokodaii
Y97A enhanced UDP-N-acetylglucosamine diphosphorylase activity under optimal conditions Sulfurisphaera tokodaii
Y97F UDP-N-acetylglucosamine diphosphorylase activity is very low Sulfurisphaera tokodaii

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 2 mM. Activation of UDP-N-acetylglucosamine diphosphorylase activity in the order of decreasing effectiveness: Co2+, Mn2+, Mg2+, Zn2+, Ca2+. No activity is detectable without a divalent cation Sulfurisphaera tokodaii
Co2+ 2 mM. Activation of UDP-N-acetylglucosamine diphosphorylase activity in the order of decreasing effectiveness: Co2+, Mn2+, Mg2+, Zn2+, Ca2+. No activity is detectable without a divalent cation Sulfurisphaera tokodaii
Mg2+ 2 mM. Activation of UDP-N-acetylglucosamine diphosphorylase activity in the order of decreasing effectiveness: Co2+, Mn2+, Mg2+, Zn2+, Ca2+. No activity is detectable without a divalent cation Sulfurisphaera tokodaii
Mn2+ 2 mM. Activation of UDP-N-acetylglucosamine diphosphorylase activity in the order of decreasing effectiveness: Co2+, Mn2+, Mg2+, Zn2+, Ca2+. No activity is detectable without a divalent cation Sulfurisphaera tokodaii
Zn2+ 2 mM. Activation of UDP-N-acetylglucosamine diphosphorylase activity in the order of decreasing effectiveness: Co2+, Mn2+, Mg2+, Zn2+, Ca2+. No activity is detectable without a divalent cation Sulfurisphaera tokodaii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Sulfurisphaera tokodaii the multifunctional enzyme is involved in biosynthesis of UDP-N-acetyl-alpha-D-glucosamine, an activated and essential form of N-acetyl-alpha-D-glucosamine that is an important component in the polysaccharide structure of most organisms ?
-
?
additional information Sulfurisphaera tokodaii 7 the multifunctional enzyme is involved in biosynthesis of UDP-N-acetyl-alpha-D-glucosamine, an activated and essential form of N-acetyl-alpha-D-glucosamine that is an important component in the polysaccharide structure of most organisms ?
-
?

Organism

Organism UniProt Comment Textmining
Sulfurisphaera tokodaii Q975F9
-
-
Sulfurisphaera tokodaii 7 Q975F9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dTTP + alpha-D-glucose 1-phosphate the enzyme also shows activity of EC 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase Sulfurisphaera tokodaii diphosphate + dTDP-alpha-D-glucose
-
?
dTTP + alpha-D-glucose 1-phosphate the enzyme also shows activity of EC 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase Sulfurisphaera tokodaii 7 diphosphate + dTDP-alpha-D-glucose
-
?
additional information the multifunctional enzyme is involved in biosynthesis of UDP-N-acetyl-alpha-D-glucosamine, an activated and essential form of N-acetyl-alpha-D-glucosamine that is an important component in the polysaccharide structure of most organisms Sulfurisphaera tokodaii ?
-
?
additional information the multifunctional enzyme is involved in biosynthesis of UDP-N-acetyl-alpha-D-glucosamine, an activated and essential form of N-acetyl-alpha-D-glucosamine that is an important component in the polysaccharide structure of most organisms Sulfurisphaera tokodaii 7 ?
-
?

Synonyms

Synonyms Comment Organism
Glc-1-P TTase
-
Sulfurisphaera tokodaii
ST0452 locus name Sulfurisphaera tokodaii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
assay at Sulfurisphaera tokodaii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
half-life: 180 min Sulfurisphaera tokodaii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Sulfurisphaera tokodaii

General Information

General Information Comment Organism
physiological function the multifunctional enzyme is involved in biosynthesis of UDP-N-acetyl-alpha-D-glucosamine, an activated and essential form of N-acetyl-alpha-D-glucosamine that is an important component in the polysaccharide structure of most organisms Sulfurisphaera tokodaii