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Literature summary for 2.7.7.24 extracted from

  • Lee, H.C.; Sohng, J.K.; Kim, H.J.; Nam, D.H.; Han, J.M.; Cho, S.S.; Choi, J.H.; Yoo, J.C.
    Cloning and expression of the glucose-1-phosphate thymidylyltransferase gene (gerD) from Streptomyces sp. GERI-155 (2004), Mol. Cells, 17, 274-280.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
x * 30000, SDS-PAGE Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
GERI-155
-
Streptomyces sp. GERI-155
-
GERI-155
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00166
-
-
Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dTTP + alpha-D-galactose 1-phosphate 12.6% of the activity with alpha-D-glucose 1-phosphate Streptomyces sp. dTDP-galactose + diphosphate
-
?
dTTP + alpha-D-galactose 1-phosphate 12.6% of the activity with alpha-D-glucose 1-phosphate Streptomyces sp. GERI-155 dTDP-galactose + diphosphate
-
?
dTTP + alpha-D-glucose 1-phosphate in the opposite direction the enzyme is highly specific for dTDP-D-glucose and diphosphate Streptomyces sp. dTDP-glucose + diphosphate
-
r
dTTP + alpha-D-glucose 1-phosphate in the opposite direction the enzyme is highly specific for dTDP-D-glucose and diphosphate Streptomyces sp. GERI-155 dTDP-glucose + diphosphate
-
r
UTP + alpha-D-glucose 1-phosphate 5.5% of the activity with dTTP Streptomyces sp. UDP-glucose + diphosphate
-
?
UTP + alpha-D-glucose 1-phosphate 5.5% of the activity with dTTP Streptomyces sp. GERI-155 UDP-glucose + diphosphate
-
?

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE Streptomyces sp.

Synonyms

Synonyms Comment Organism
glucose-1-phosphate thymidylyltransferase
-
Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
34 37
-
Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8.5
-
Streptomyces sp.