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Literature summary for 2.7.7.24 extracted from

  • Silva, E.; Marques, A.R.; Fialho, A.M.; Granja, A.T.; Sa-Correia, I.
    Proteins encoded by Sphingomonas elodea ATCC 31461 rmlA and ugpG genes, involved in gellan gum biosynthesis, exhibit both dTDP- and UDP-glucose pyrophosphorylase activities (2005), Appl. Environ. Microbiol., 71, 4703-4712.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, RmlA protein Sphingomonas elodea
expression in Escherichia coli, UgpG protein Sphingomonas elodea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0094
-
dTDP-glucose UgpG protein Sphingomonas elodea
0.012
-
dTDP-glucose RmlA protein Sphingomonas elodea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dTDP-glucose + diphosphate Sphingomonas elodea protein by ugpG gene is involved in gellan gum biosynthesis and exhibits both dTDP- and UDP-glucose diphosphorylase activities dTTP + alpha-D-glucose 1-phosphate
-
?
dTDP-glucose + diphosphate Sphingomonas elodea protein encoded by rmlA gene is involved in gellan gum biosynthesis and exhibits both dTDP- and UDP-glucose diphosphorylase activities dTTP + alpha-D-glucose 1-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Sphingomonas elodea
-
ATCC 31461
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, RmlA protein Sphingomonas elodea
recombinant enzyme, UgpG protein Sphingomonas elodea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dTDP-glucose + diphosphate
-
Sphingomonas elodea dTTP + alpha-D-glucose 1-phosphate
-
?
dTDP-glucose + diphosphate protein by ugpG gene is involved in gellan gum biosynthesis and exhibits both dTDP- and UDP-glucose diphosphorylase activities Sphingomonas elodea dTTP + alpha-D-glucose 1-phosphate
-
?
dTDP-glucose + diphosphate protein encoded by rmlA gene is involved in gellan gum biosynthesis and exhibits both dTDP- and UDP-glucose diphosphorylase activities Sphingomonas elodea dTTP + alpha-D-glucose 1-phosphate
-
?

Synonyms

Synonyms Comment Organism
RmlA
-
Sphingomonas elodea
RmlA protein protein encoded by rmlA gene is involved in gellan gum biosynthesis and exhibits both dTDP- and UDP-glucose diphosphorylase activities Sphingomonas elodea
ugpG
-
Sphingomonas elodea
UgpG protein protein encoded by ugpG gene is involved in gellan gum biosynthesis and exhibits both dTDP- and UDP-glucose diphosphorylase activities Sphingomonas elodea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.56
-
dTDP-glucose UgpG protein Sphingomonas elodea
1.35
-
dTDP-glucose RmlA protein Sphingomonas elodea