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Literature summary for 2.7.7.24 extracted from

  • Singh, B.; Lee, C.; Park, J.; Sohng, J.
    The amino acid sequences in the C-terminal region of glucose-1-phosphate thymidylyltransferases determine their soluble expression in Escherichia coli (2012), Protein Eng. Des. Sel., 25, 179-187.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, protein forms inclusion bodies Streptomyces peucetius

Crystallization (Commentary)

Crystallization (Comment) Organism
homology modeling of both isoforms DnmL and RmbA Streptomyces peucetius

Protein Variants

Protein Variants Comment Organism
additional information deletion of the C-terminal sequence of isoform DnmL increases the expression level of truncated DnmL, albeit without enzymic activity. Substitution of the C-terminal sequence of DnmL with that of isoform RmbA also leads to expression of the recombinant protein in soluble form. The fusion protein is catalytically active Streptomyces peucetius
R313S/T317V/H322A/A339S/S341H/D348H mutation of residues to the corresponing residues of isoform RmbA, giving rise to highly similar hydrophobicity pattern with RmbA. Mutations render the protein expressed in soluble form, the mutant is catalytically active Streptomyces peucetius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.66
-
dTTP pH 7.2, 37°C, fusion protein of isoform DnmL with C-terminal sequence of isoform RmbA Streptomyces peucetius
1.72
-
dTTP pH 7.2, 37°, mutant R313S/T317V/H322A/A339S/S341H/D348H Streptomyces peucetius

Organism

Organism UniProt Comment Textmining
Streptomyces peucetius C6K8M0
-
-
Streptomyces peucetius ATCC 27952 C6K8M0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dTTP + alpha-D-glucose 1-phosphate
-
Streptomyces peucetius dTDP-alpha-D-glucose + diphosphate
-
?
dTTP + alpha-D-glucose 1-phosphate
-
Streptomyces peucetius ATCC 27952 dTDP-alpha-D-glucose + diphosphate
-
?

Synonyms

Synonyms Comment Organism
DnmL
-
Streptomyces peucetius

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1
-
dTTP pH 7.2, 37°, mutant R313S/T317V/H322A/A339S/S341H/D348H Streptomyces peucetius
1.15
-
dTTP pH 7.2, 37°C, fusion protein of isoform DnmL with C-terminal sequence of isoform RmbA Streptomyces peucetius