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Literature summary for 2.7.7.24 extracted from

  • Han, J.; Kim, S.; Lee, H.; Yoo, J.
    Cloning and expression of glucose-1-phosphate thymidylyltransferase gene (schS6) from Streptomyces sp. SCC-2136 (2007), J. Microbiol. Biotechnol., 17, 685-690.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli vector pSCHS6 Streptomyces sp.
expressed in Escherichia coli, vector pSCHS6 Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
SDS-PAGE Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
-
-
Streptomyces sp. Q2HR55
-
-
Streptomyces sp. SCC-2136
-
-
-
Streptomyces sp. SCC-2136 Q2HR55
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and Ni-NTA affinity column chromatography Streptomyces sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00085
-
crude extract, at pH 8.0 and 37°C Streptomyces sp.
0.004
-
after 4.7fold purification, at pH 8.0 and 37°C Streptomyces sp.
0.017
-
crude cell extract Streptomyces sp.
0.0801
-
after 4.7fold purification Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + alpha-D-glucose 1-phosphate 1% activity compared to dTTP Streptomyces sp. ADP-glucose + diphosphate
-
r
ATP + alpha-D-glucose 1-phosphate 1% activity compared to dTTP Streptomyces sp. SCC-2136 ADP-glucose + diphosphate
-
r
ATP + alpha-D-glucose-1-phosphate 1.0% of the activity with dTTP and alpha-D-glucose 1-phosphate Streptomyces sp. ADP-glucose + diphosphate
-
?
ATP + alpha-D-glucose-1-phosphate 1.0% of the activity with dTTP and alpha-D-glucose 1-phosphate Streptomyces sp. SCC-2136 ADP-glucose + diphosphate
-
?
CTP + alpha-D-glucose 1-phosphate 1.2% activity compared to dTTP Streptomyces sp. CDP-glucose + diphosphate
-
r
CTP + alpha-D-glucose-1-phosphate 1.2% of the activity with dTTP and alpha-D-glucose 1-phosphate Streptomyces sp. CDP-glucose + diphosphate
-
?
CTP + alpha-D-glucose-1-phosphate 1.2% of the activity with dTTP and alpha-D-glucose 1-phosphate Streptomyces sp. SCC-2136 CDP-glucose + diphosphate
-
?
dTTP + alpha-D-galactose 1-phosphate 10% activity compared to alpha-D-glucose 1-phosphate Streptomyces sp. dTDP-galactose + diphosphate
-
r
dTTP + alpha-D-galactose 1-phosphate 10% activity compared to alpha-D-glucose 1-phosphate Streptomyces sp. SCC-2136 dTDP-galactose + diphosphate
-
r
dTTP + alpha-D-galactose-1-phosphate 10% of the activity with dTTP and alpha-D-glucose 1-phosphate Streptomyces sp. dTDP-galactose + diphosphate
-
?
dTTP + alpha-D-glucosamine 1-phosphate 23% activity compared to alpha-D-glucose 1-phosphate Streptomyces sp. dTDP-glucosamine + diphosphate
-
r
dTTP + alpha-D-glucosamine-1-phosphate 23% of the activity with dTTP and alpha-D-glucose 1-phosphate Streptomyces sp. dTDP-glucosamine + diphosphate
-
?
dTTP + alpha-D-glucose 1-phosphate
-
Streptomyces sp. dTDP-glucose + diphosphate highly specific for dTDP-glucose and diphosphate r
dTTP + alpha-D-glucose 1-phosphate 100% activity Streptomyces sp. dTDP-glucose + diphosphate
-
r
dTTP + alpha-D-glucose 1-phosphate
-
Streptomyces sp. SCC-2136 dTDP-glucose + diphosphate highly specific for dTDP-glucose and diphosphate r
dTTP + alpha-D-glucose 1-phosphate 100% activity Streptomyces sp. SCC-2136 dTDP-glucose + diphosphate
-
r
dTTP + alpha-D-mannose 1-phosphate 11% activity compared to alpha-D-glucose 1-phosphate Streptomyces sp. dTDP-mannose + diphosphate
-
r
dTTP + alpha-D-mannose-1-phosphate 11% of the activity with dTTP and alpha-D-glucose 1-phosphate Streptomyces sp. dTDP-mannose + diphosphate
-
?
additional information GTP, UTP/alpha-D-mannose-1-phosphate or UTP/alpha-D-galactose-1-phosphate are no substrates Streptomyces sp. ?
-
?
additional information the enzyme shows 102% relative activity for dTMP or dTDP compared to dTTP at 2 mM, respectively Streptomyces sp. ?
-
?
additional information almost no activity with ATP-D-glucose, CTP-D-glucose, UTP-D-Glucose, GTP, alpha-D-galactose-1-phosphate, and alpha-D-mannose-1-phosphate Streptomyces sp. ?
-
?
additional information GTP, UTP/alpha-D-mannose-1-phosphate or UTP/alpha-D-galactose-1-phosphate are no substrates Streptomyces sp. SCC-2136 ?
-
?
additional information the enzyme shows 102% relative activity for dTMP or dTDP compared to dTTP at 2 mM, respectively Streptomyces sp. SCC-2136 ?
-
?
additional information almost no activity with ATP-D-glucose, CTP-D-glucose, UTP-D-Glucose, GTP, alpha-D-galactose-1-phosphate, and alpha-D-mannose-1-phosphate Streptomyces sp. SCC-2136 ?
-
?
UTP + alpha-D-glucosamine 1-phosphate 8% activity compared to the reaction with alpha-D-glucose 1-phosphate and dTTP Streptomyces sp. UDP-glucosamine + diphosphate
-
r
UTP + alpha-D-glucosamine-1-phosphate 8% of the activity with dTTP and alpha-D-glucose 1-phosphate Streptomyces sp. UDP-glucosamine + diphosphate
-
?
UTP + alpha-D-glucose 1-phosphate 12% activity compared to dTTP Streptomyces sp. UDP-glucose + diphosphate
-
r
UTP + alpha-D-glucose 1-phosphate 12% activity compared to dTTP Streptomyces sp. SCC-2136 UDP-glucose + diphosphate
-
r
UTP + alpha-D-glucose-1-phosphate 12% of the activity with dTTP and alpha-D-glucose 1-phosphate Streptomyces sp. UDP-glucose + diphosphate
-
?

Synonyms

Synonyms Comment Organism
dTDP-D-glucose synthase
-
Streptomyces sp.
glucose-1-phosphate thymidylyltransferase
-
Streptomyces sp.
SchS6
-
Streptomyces sp.