Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli vector pSCHS6 | Streptomyces sp. |
expressed in Escherichia coli, vector pSCHS6 | Streptomyces sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
SDS-PAGE | Streptomyces sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | - |
- |
- |
Streptomyces sp. | Q2HR55 | - |
- |
Streptomyces sp. SCC-2136 | - |
- |
- |
Streptomyces sp. SCC-2136 | Q2HR55 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and Ni-NTA affinity column chromatography | Streptomyces sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00085 | - |
crude extract, at pH 8.0 and 37°C | Streptomyces sp. |
0.004 | - |
after 4.7fold purification, at pH 8.0 and 37°C | Streptomyces sp. |
0.017 | - |
crude cell extract | Streptomyces sp. |
0.0801 | - |
after 4.7fold purification | Streptomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-D-glucose 1-phosphate | 1% activity compared to dTTP | Streptomyces sp. | ADP-glucose + diphosphate | - |
r | |
ATP + alpha-D-glucose 1-phosphate | 1% activity compared to dTTP | Streptomyces sp. SCC-2136 | ADP-glucose + diphosphate | - |
r | |
ATP + alpha-D-glucose-1-phosphate | 1.0% of the activity with dTTP and alpha-D-glucose 1-phosphate | Streptomyces sp. | ADP-glucose + diphosphate | - |
? | |
ATP + alpha-D-glucose-1-phosphate | 1.0% of the activity with dTTP and alpha-D-glucose 1-phosphate | Streptomyces sp. SCC-2136 | ADP-glucose + diphosphate | - |
? | |
CTP + alpha-D-glucose 1-phosphate | 1.2% activity compared to dTTP | Streptomyces sp. | CDP-glucose + diphosphate | - |
r | |
CTP + alpha-D-glucose-1-phosphate | 1.2% of the activity with dTTP and alpha-D-glucose 1-phosphate | Streptomyces sp. | CDP-glucose + diphosphate | - |
? | |
CTP + alpha-D-glucose-1-phosphate | 1.2% of the activity with dTTP and alpha-D-glucose 1-phosphate | Streptomyces sp. SCC-2136 | CDP-glucose + diphosphate | - |
? | |
dTTP + alpha-D-galactose 1-phosphate | 10% activity compared to alpha-D-glucose 1-phosphate | Streptomyces sp. | dTDP-galactose + diphosphate | - |
r | |
dTTP + alpha-D-galactose 1-phosphate | 10% activity compared to alpha-D-glucose 1-phosphate | Streptomyces sp. SCC-2136 | dTDP-galactose + diphosphate | - |
r | |
dTTP + alpha-D-galactose-1-phosphate | 10% of the activity with dTTP and alpha-D-glucose 1-phosphate | Streptomyces sp. | dTDP-galactose + diphosphate | - |
? | |
dTTP + alpha-D-glucosamine 1-phosphate | 23% activity compared to alpha-D-glucose 1-phosphate | Streptomyces sp. | dTDP-glucosamine + diphosphate | - |
r | |
dTTP + alpha-D-glucosamine-1-phosphate | 23% of the activity with dTTP and alpha-D-glucose 1-phosphate | Streptomyces sp. | dTDP-glucosamine + diphosphate | - |
? | |
dTTP + alpha-D-glucose 1-phosphate | - |
Streptomyces sp. | dTDP-glucose + diphosphate | highly specific for dTDP-glucose and diphosphate | r | |
dTTP + alpha-D-glucose 1-phosphate | 100% activity | Streptomyces sp. | dTDP-glucose + diphosphate | - |
r | |
dTTP + alpha-D-glucose 1-phosphate | - |
Streptomyces sp. SCC-2136 | dTDP-glucose + diphosphate | highly specific for dTDP-glucose and diphosphate | r | |
dTTP + alpha-D-glucose 1-phosphate | 100% activity | Streptomyces sp. SCC-2136 | dTDP-glucose + diphosphate | - |
r | |
dTTP + alpha-D-mannose 1-phosphate | 11% activity compared to alpha-D-glucose 1-phosphate | Streptomyces sp. | dTDP-mannose + diphosphate | - |
r | |
dTTP + alpha-D-mannose-1-phosphate | 11% of the activity with dTTP and alpha-D-glucose 1-phosphate | Streptomyces sp. | dTDP-mannose + diphosphate | - |
? | |
additional information | GTP, UTP/alpha-D-mannose-1-phosphate or UTP/alpha-D-galactose-1-phosphate are no substrates | Streptomyces sp. | ? | - |
? | |
additional information | the enzyme shows 102% relative activity for dTMP or dTDP compared to dTTP at 2 mM, respectively | Streptomyces sp. | ? | - |
? | |
additional information | almost no activity with ATP-D-glucose, CTP-D-glucose, UTP-D-Glucose, GTP, alpha-D-galactose-1-phosphate, and alpha-D-mannose-1-phosphate | Streptomyces sp. | ? | - |
? | |
additional information | GTP, UTP/alpha-D-mannose-1-phosphate or UTP/alpha-D-galactose-1-phosphate are no substrates | Streptomyces sp. SCC-2136 | ? | - |
? | |
additional information | the enzyme shows 102% relative activity for dTMP or dTDP compared to dTTP at 2 mM, respectively | Streptomyces sp. SCC-2136 | ? | - |
? | |
additional information | almost no activity with ATP-D-glucose, CTP-D-glucose, UTP-D-Glucose, GTP, alpha-D-galactose-1-phosphate, and alpha-D-mannose-1-phosphate | Streptomyces sp. SCC-2136 | ? | - |
? | |
UTP + alpha-D-glucosamine 1-phosphate | 8% activity compared to the reaction with alpha-D-glucose 1-phosphate and dTTP | Streptomyces sp. | UDP-glucosamine + diphosphate | - |
r | |
UTP + alpha-D-glucosamine-1-phosphate | 8% of the activity with dTTP and alpha-D-glucose 1-phosphate | Streptomyces sp. | UDP-glucosamine + diphosphate | - |
? | |
UTP + alpha-D-glucose 1-phosphate | 12% activity compared to dTTP | Streptomyces sp. | UDP-glucose + diphosphate | - |
r | |
UTP + alpha-D-glucose 1-phosphate | 12% activity compared to dTTP | Streptomyces sp. SCC-2136 | UDP-glucose + diphosphate | - |
r | |
UTP + alpha-D-glucose-1-phosphate | 12% of the activity with dTTP and alpha-D-glucose 1-phosphate | Streptomyces sp. | UDP-glucose + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dTDP-D-glucose synthase | - |
Streptomyces sp. |
glucose-1-phosphate thymidylyltransferase | - |
Streptomyces sp. |
SchS6 | - |
Streptomyces sp. |