Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli BL21 with a 6 x His-tag | Pseudomonas aeruginosa |
Crystallization (Comment) | Organism |
---|---|
sitting-drop vapour-diffusion method in polyethylene glycol 6000, 0.1 M sodium citrate, pH 4.6 and 0.5 M Li2SO4 as precipitant, space group: P1, the asymmetric unit contains eight monomers in the form of two RmlA tetramers with a solvent content of 51% | Pseudomonas aeruginosa |
sitting-drop vapour-diffusion technique with PEG 6000 and lithium sulfate as precipitant. Several diffraction data sets of single frozen crystals are collected to a resolution of 1.66 A. Crystals belong to space group P1, with unit-cell parameters a = 71.5 A, b = 73.1 A, c = 134.7 A, alpha = 89.9°, beta = 80.9°, gamma = 81° | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33770 | - |
calculated from nucleic acid sequence | Pseudomonas aeruginosa |
33800 | - |
MALDI mass spectrum | Pseudomonas aeruginosa |
106000 | 122000 | dynamic light scattering | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTTP + alpha-D-glucose 1-phosphate | Pseudomonas aeruginosa | - |
dTDP-glucose + diphosphate | - |
? | |
dTTP + alpha-D-glucose 1-phosphate | Pseudomonas aeruginosa | first enzyme of the dTDP-L-rhamnose synthesis pathway | dTDP-glucose + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
recombinant enzyme with his-tag | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTTP + alpha-D-glucose 1-phosphate | - |
Pseudomonas aeruginosa | dTDP-glucose + diphosphate | - |
? | |
dTTP + alpha-D-glucose 1-phosphate | first enzyme of the dTDP-L-rhamnose synthesis pathway | Pseudomonas aeruginosa | dTDP-glucose + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | trimer or tetramer | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
glucose-1-phosphate thymidylyltransferase | - |
Pseudomonas aeruginosa |
RmlA | - |
Pseudomonas aeruginosa |