Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.24 extracted from

  • Blankenfeldt, W.; Giraud, M.F.; Leonard, G.; Rahim, R.; Creuzenet, C.; Lam, J.S.; Naismith, J.H.
    The purification, crystallization and preliminary structural characterization of glucose-1-phosphate thymidylyltransferase (RmlA), the first enzyme of the dTDP-L-rhamnose synthesis pathway from Pseudomonas aeruginosa (2000), Acta Crystallogr. Sect. D, 56, 1501-1504.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli BL21 with a 6 x His-tag Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting-drop vapour-diffusion method in polyethylene glycol 6000, 0.1 M sodium citrate, pH 4.6 and 0.5 M Li2SO4 as precipitant, space group: P1, the asymmetric unit contains eight monomers in the form of two RmlA tetramers with a solvent content of 51% Pseudomonas aeruginosa
sitting-drop vapour-diffusion technique with PEG 6000 and lithium sulfate as precipitant. Several diffraction data sets of single frozen crystals are collected to a resolution of 1.66 A. Crystals belong to space group P1, with unit-cell parameters a = 71.5 A, b = 73.1 A, c = 134.7 A, alpha = 89.9°, beta = 80.9°, gamma = 81° Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33770
-
calculated from nucleic acid sequence Pseudomonas aeruginosa
33800
-
MALDI mass spectrum Pseudomonas aeruginosa
106000 122000 dynamic light scattering Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dTTP + alpha-D-glucose 1-phosphate Pseudomonas aeruginosa
-
dTDP-glucose + diphosphate
-
?
dTTP + alpha-D-glucose 1-phosphate Pseudomonas aeruginosa first enzyme of the dTDP-L-rhamnose synthesis pathway dTDP-glucose + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas aeruginosa
recombinant enzyme with his-tag Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dTTP + alpha-D-glucose 1-phosphate
-
Pseudomonas aeruginosa dTDP-glucose + diphosphate
-
?
dTTP + alpha-D-glucose 1-phosphate first enzyme of the dTDP-L-rhamnose synthesis pathway Pseudomonas aeruginosa dTDP-glucose + diphosphate
-
?

Subunits

Subunits Comment Organism
More trimer or tetramer Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
glucose-1-phosphate thymidylyltransferase
-
Pseudomonas aeruginosa
RmlA
-
Pseudomonas aeruginosa