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Literature summary for 2.7.7.24 extracted from

  • Zuccotti, S.; Zanardi, D.; Rosano, C.; Sturla, L.; Tonetti, M.; Bolognesi, M.
    Kinetic and crystallographic analyses support a sequential-ordered bi bi catalytic mechanism for Escherichia coli glucose-1-phosphate thymidylyltransferase (2001), J. Mol. Biol., 313, 831-843.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli as GST-fusion protein Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
co-crystallization with dTDP-glucose or dTMP and alpha-D-glucose 1-phosphate or deoxythymidine and alpha-D-glucose 1-phosphate, hanging-drop vapour-diffusion method in 1.7 M ammonium sulfate and 0.1 M sodium acetate, pH 5.3 Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
dTDP-glucose competitive to dTTP Escherichia coli
dTTP competitive to dTDPglucose and non-competitive to alpha-D-glucose 1-phosphate Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0205
-
dTTP 37°C pH 8 Escherichia coli
0.034
-
alpha-D-glucose 1-phosphate 37°C pH 8 Escherichia coli
0.095
-
dTDP-glucose 37°C pH 8 Escherichia coli
0.154
-
diphosphate 37°C pH 8 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ absolute requirement, 2 mM: optimal Escherichia coli
Mn2+
-
Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dTTP + alpha-D-glucose 1-phosphate Escherichia coli
-
dTDP-glucose + diphosphate
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
194
-
forward reaction Escherichia coli
360
-
reverse reaction Escherichia coli

Storage Stability

Storage Stability Organism
-20°C at concentrations higher than 5 mg/ml stable for up to three months Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dTTP + alpha-D-glucose 1-phosphate
-
Escherichia coli dTDP-glucose + diphosphate
-
r

Subunits

Subunits Comment Organism
tetramer alpha4, crystallization studies Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5 broad Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
6 10
-
Escherichia coli