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6.4.1.2: acetyl-CoA carboxylase

This is an abbreviated version!
For detailed information about acetyl-CoA carboxylase, go to the full flat file.

Word Map on EC 6.4.1.2

Reaction

ATP
+
acetyl-CoA
 +
hydrogencarbonate
=
ADP
 +
phosphate
 +
malonyl-CoA

Synonyms

ACACA, ACACB, ACC, ACC-1, ACC-2, ACC1, Acc1a, Acc1b, Acc1p, ACC2, AccA, ACCalpha, ACCase, ACCase 1, ACCB, AccC, Acetyl CoA carboxylase, Acetyl coenzyme A carboxylase, acetyl coenzyme A carboxylase alpha, acetyl-CoA carboxylase, acetyl-CoA carboxylase 1, acetyl-CoA carboxylase 2, acetyl-CoA carboxylase A, acetyl-CoA carboxylase alpha, acetyl-CoA carboxylase B, acetyl-CoA carboxylase-2, acetyl-CoA carboxylase-alpha, acetyl-CoA-carboxylase, acetyl-CoA/propionyl-CoA carboxylase, Acetyl-coenzyme A carboxylase, acetyl-coenzyme-A carboxylase, acetylCoA carboxylase, acyl coenzyme A carboxylase, acyl-CoA carboxylase, BCCP1, BCCP2, CAC1A, CAC1B, Carboxylase, acetyl coenzyme A, Hfa1p, More, PccB

ECTree

     6 Ligases
         6.4 Forming carbon-carbon bonds
             6.4.1 Ligases that form carbon-carbon bonds (only sub-subclass identified to date)
                6.4.1.2 acetyl-CoA carboxylase

Purification

Purification on EC 6.4.1.2 - acetyl-CoA carboxylase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 isoforms: non-mesophyll chloroplast form and mesophyll chloroplast form
-
478fold by ammonium sulfate fractionation, ion exchange chromatography, ultrafiltration, and gel filtration
-
acetyl-CoA carboxylase I and acetyl-CoA carboxylase II
-
beta subunit of the carboxyltransferase
-
c-Myc-5 monoclonal IgG column chromatography and Sephacryl S-300 gel filtration
hindlimb muscle
-
large-scale purification of of intact 156-residue biotin carboxyl carrier subunit of the acetyl-CoA carboxylase and the truncated 87000 residue COOH-terminal fragment
-
native enzyme from liver by two steps of ammonium sulfate fractionation, and anion exchange chromatography
-
Ni affinity resin column chromatography, Q Sepharose HP column chromatography, and Superdex 200 gel filtration
Ni-affinity column chromatography and gel filtration
of the biotin-domain peptide
-
partial
partial purification of the carboxyltransferase subunit
-
partially from liver by avidin affinity chromatography including a batch step
-
partially from young leaves by ammonium sulfate fractionation
rapid purification is of particular use in the isolation of acetyl CoA carboxylase from plant tissues which are prone to proteolysis
-
recombinant CT domain comprising residues 1476-2233
recombinant enzyme from Sf9 insect cells by ammonium sulfate fractionation and anion exchange chromatography
-
recombinant full length enzyme and His-tagged BC domain from Escherichia coli, the latter by nickel affinity chromatography
recombinant His-tagged biotin carboxylase domain from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, anion exchange chromatography, and gel filtration
recombinant His-tagged carboxyltransferase domain comprising residues 1548-2346 from Escherichia coli by nickel affinity and ion exchange chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain XL 1-Blue by nickel affinity chromatography
-
recombinant His-tagged enzyme subunits AccA and AccD from Escherichia coli strain XL 1-Blue by nickel affinity chromatography
-
recombinant His-tagged enzyme subunits AccA and AccD from strain XL 1-Blue by nickel affinity chromatography
-
recombinant protein from Escherichia coli using His-tag
resource Q column chromatography
-
separation of two isoforms
Sephadex G-25 gel filtration
-
Streptavidin column chromatography and Superose 6 gel filtration
Q877I4; Q877I5; Q877I3
using Ni-NTA chromatography