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Literature summary for 6.4.1.2 extracted from
- Expression and characterization of recombinant fungal acetyl-CoA carboxylase and isolation of a soraphen-binding domain (2004), Biochem. J., 380, 105-110.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| biotin | - |
Pyricularia grisea |  |
| biotin | - |
Phytophthora infestans | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of the BC domains in Escherichia coli strain BL21(DE3) | Pyricularia grisea |
| expression of the BC domains in Escherichia coli strain BL21(DE3) | Phytophthora infestans |
| functional expression of the full length enzyme, and expression of the His-tagged BC domain in Escherichia coli | Ustilago maydis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| soraphen A | noncompetitive, binds to the BC domain | Phytophthora infestans | |
| soraphen A | noncompetitive, binds to the BC domain | Pyricularia grisea | |
| soraphen A | noncompetitive, binds to the BC domain | Ustilago maydis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.14 | - |
acetyl-CoA | pH 8.0, 30°C, recombinant enzyme | Ustilago maydis | |
| 0.19 | - |
ATP | pH 8.0, 30°C, recombinant enzyme | Ustilago maydis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Pyricularia grisea | |
| Mg2+ | - |
Ustilago maydis | |
| Mg2+ | - |
Phytophthora infestans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
x * 65000, recombinant BC domain, SDS-PAGE | Ustilago maydis |
| 66000 | - |
recombinant BC domain, gel filtration | Ustilago maydis |
| 225000 | - |
4 * 225000, recombinant enzyme, SDS-PAGE | Ustilago maydis |
| 880000 | - |
approximately, recombinant enzyme, gel filtration | Ustilago maydis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + acetyl-CoA + HCO3- | Pyricularia grisea | - |
ADP + malonyl-CoA + phosphate | - |
? | |
| ATP + acetyl-CoA + HCO3- | Phytophthora infestans | - |
ADP + malonyl-CoA + phosphate | - |
? | |
| ATP + acetyl-CoA + HCO3- | Ustilago maydis | the enzyme catalyzes the first step in fatty acid bioynthesis | ADP + malonyl-CoA + phosphate | - |
? | |
| ATP + acetyl-CoA + HCO3- | Pyricularia grisea Guy11 | - |
ADP + malonyl-CoA + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phytophthora infestans | Q6SZP8 | genotype US-1 | - |
| Pyricularia grisea | - |
- |
- |
| Pyricularia grisea Guy11 | - |
- |
- |
| Ustilago maydis | Q12721 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| additional information | the enzyme is biotinylated | Pyricularia grisea |
| additional information | the enzyme is biotinylated | Ustilago maydis |
| additional information | the enzyme is biotinylated | Phytophthora infestans |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant full length enzyme and His-tagged BC domain from Escherichia coli, the latter by nickel affinity chromatography | Ustilago maydis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.3 | - |
purified recombinant enzyme | Ustilago maydis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + acetyl-CoA + HCO3- | - |
Pyricularia grisea | ADP + malonyl-CoA + phosphate | - |
? | |
| ATP + acetyl-CoA + HCO3- | - |
Ustilago maydis | ADP + malonyl-CoA + phosphate | - |
? | |
| ATP + acetyl-CoA + HCO3- | - |
Phytophthora infestans | ADP + malonyl-CoA + phosphate | - |
? | |
| ATP + acetyl-CoA + HCO3- | the enzyme catalyzes the first step in fatty acid bioynthesis | Ustilago maydis | ADP + malonyl-CoA + phosphate | - |
? | |
| ATP + acetyl-CoA + HCO3- | - |
Pyricularia grisea Guy11 | ADP + malonyl-CoA + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 65000, recombinant BC domain, SDS-PAGE | Ustilago maydis |
| tetramer | 4 * 225000, recombinant enzyme, SDS-PAGE | Ustilago maydis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ACC | - |
Pyricularia grisea |
| ACC | - |
Ustilago maydis |
| ACC | - |
Phytophthora infestans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Pyricularia grisea |
| 30 | - |
assay at | Ustilago maydis |
| 30 | - |
assay at | Phytophthora infestans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Pyricularia grisea |
| 8 | - |
assay at | Ustilago maydis |
| 8 | - |
assay at | Phytophthora infestans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Pyricularia grisea | |
| ATP | - |
Ustilago maydis | |
| ATP | - |
Phytophthora infestans | |
| biotin | covalently bound prosthetic group, required for activity | Ustilago maydis | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | soraphen A dissociation constant is 0.8 nM | Ustilago maydis | |
| 0.0000021 | - |
soraphen A | recombinant enzyme, pH 8.0, 30°C | Ustilago maydis | |
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