6.3.5.5: carbamoyl-phosphate synthase (glutamine-hydrolysing)

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For detailed information about carbamoyl-phosphate synthase (glutamine-hydrolysing), go to the full flat file.

Reaction

2 ATP +

Synonyms

CAD, CAD carbamoyl-phosphate synthetase, CAD protein, Carbamoyl phosphate synthase (glutamine), carbamoyl phosphate synthetase, Carbamoyl phosphate synthetase (glutamine-hydrolyzing), carbamoyl phosphate synthetase 1, carbamoyl phosphate synthetase II, carbamoyl phosphate synthetase III, carbamoyl-phosphate synthetase, Carbamoyl-phosphate synthetase (glutamine-hydrolysing), carbamoyl-phosphate synthetase 2, Carbamoylphosphate synthase, Carbamoylphosphate synthetase, Carbamoylphosphate synthetase II, Carbamyl phosphate synthetase (glutamine), Carbamyl phosphate sythetase II, carbamylphosphate synthetase - aspartate transcarbamylase, CPA2, CPS, CPS II (glutamine-dependent), CPS III, CPS III (glutamine- and N-acetyl-L-glutamine-dependent), CPS1, CPSase, CPSase type II, CPSase-A, CPSase-P, CPSII, EC 2.7.2.9, Glutamine-dependent carbamyl phosphate synthetase, glutamine-hydrolyzing CPSase, MGG_04503, More, MtCPSs1, MtCPSs2, Synthase, carbamoylphosphate (glutamine), Synthetase, carbamoylphosphate (glutamine-hydrolyzing)

ECTree

     6 Ligases

         6.3 Forming carbon-nitrogen bonds

             6.3.5 Carbon-nitrogen ligases with glutamine as amido-N-donor

                6.3.5.5 carbamoyl-phosphate synthase (glutamine-hydrolysing)

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66	Activating Compound
87162	AA Sequence
2	Application
1	CAS Registry Number
28	Cloned(Commentary)
22	Cofactor
8	Crystallization (Commentary)
76	Disease/ Diagnostics
3	Expression
9	General Information
2	General Stability
4	IC50 Value
100	Inhibitors
3	Ki Value [mM]
200	KM Value [mM]
14	Localization
21	Metals/Ions
43	Molecular Weight [Da]
56	Natural Substrates/ Products (Substrates)
490	Organism
1	Oxidation Stability
12	Pathway
91	PDB ID
17	pH Optimum
2	pH Range
1	pH Stability
5	Posttranslational Modification
209	Protein Variants
28	Purification (Commentary)
27	Reaction
3	Reaction Type
137	Reference
36	Source Tissue
17	Specific Activity [micromol/min/mg]
2	Storage Stability
219	Substrates and Products (Substrate)
46	Subunits
73	Synonyms
1	Systematic Name
6	Temperature Optimum [°C]
3	Temperature Stability [°C]
145	Turnover Number [1/s]

Systematic Name

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Systematic Name on EC 6.3.5.5 - carbamoyl-phosphate synthase (glutamine-hydrolysing)

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SYSTEMATIC NAME

IUBMB Comments

hydrogen-carbonate:L-glutamine amido-ligase (ADP-forming, carbamate-phosphorylating)

The product carbamoyl phosphate is an intermediate in the biosynthesis of arginine and the pyrimidine nucleotides [4]. The enzyme from Escherichia coli has three separate active sites, which are connected by a molecular tunnel that is almost 100 A in length [8]. The amidotransferase domain within the small subunit of the enzyme hydrolyses glutamine to ammonia via a thioester intermediate. The ammonia migrates through the interior of the protein, where it reacts with carboxyphosphate to produce the carbamate intermediate. The carboxyphosphate intermediate is formed by the phosphorylation of hydrogencarbonate by ATP at a site contained within the N-terminal half of the large subunit. The carbamate intermediate is transported through the interior of the protein to a second site within the C-terminal half of the large subunit, where it is phosphorylated by another ATP to yield the final product, carbamoyl phosphate [6]. cf. EC 6.3.4.16, carbamoyl-phosphate synthase (ammonia).