Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
additional information
-
0.01
ADP
mutant M911E, value below 0.01, ATP-synthesis, pH 7.6, temperature not specified in the publication
0.024
ADP
mutant L648E, ATP-synthesis, pH 7.6, temperature not specified in the publication
0.031
ADP
mutant L720E, ATP-synthesis, pH 7.6, temperature not specified in the publication
0.058
ADP
mutant G575F, ATP-synthesis, pH 7.6, temperature not specified in the publication
0.086
ADP
mutant I18W/A23F/C24F, ATP-synthesis, pH 7.6, temperature not specified in the publication
0.1
ADP
mutant M174E, ATP-synthesis, pH 7.6, temperature not specified in the publication
0.13
ADP
wild-type, ATP-synthesis, pH 7.6, temperature not specified in the publication
0.15
ADP
mutant G575K, ATP-synthesis, pH 7.6, temperature not specified in the publication
0.2
ADP
mutant M378E, ATP-synthesis, pH 7.6, temperature not specified in the publication
0.22
ADP
mutant M174E/M378E, ATP-synthesis, pH 7.6, temperature not specified in the publication
0.23
ADP
mutant A23F, ATP-synthesis, pH 7.6, temperature not specified in the publication
0.32
ADP
mutant A23K, ATP-synthesis, pH 7.6, temperature not specified in the publication
0.006
ATP
mutant I18W/A23F/C24F, Gln-dependent ATPase activity, pH 7.6, temperature not specified in the publication
0.01
ATP
mutant I18W/A23F/C24F, value below 0.01, CP-synthesis (co-substrate: L-Gln, HCO3-), pH 7.6, temperature not specified in the publication
0.01
ATP
mutant L648E,value below 0.01, CP-synthesis (co-substrate: L-Gln, HCO3-), pH 7.6, temperature not specified in the publication
0.01
ATP
mutant L720E, value below 0.01, CP-synthesis (co-substrate: L-Gln, HCO3-), pH 7.6, temperature not specified in the publication
0.01
ATP
mutant M174E/M378E, value below 0.01, CP-synthesis (co-substrate: L-Gln, HCO3-), pH 7.6, temperature not specified in the publication
0.012
ATP
mutant M378E, CP-synthesis (co-substrate: L-Gln, HCO3-), pH 7.6, temperature not specified in the publication
0.013
ATP
mutant M378E, HCO3-dependent ATPase activity, pH 7.6, temperature not specified in the publication
0.015
ATP
mutant M911E, value below 0.01, CP-synthesis (co-substrate: L-Gln, HCO3-), pH 7.6, temperature not specified in the publication
0.028
ATP
mutant M174E/M378E, HCO3-dependent ATPase activity, pH 7.6, temperature not specified in the publication
0.033
ATP
mutant A23F, CP-synthesis (co-substrate: L-Gln, HCO3-), pH 7.6, temperature not specified in the publication
0.035
ATP
mutant M378E, Gln-dependent ATPase activity, pH 7.6, temperature not specified in the publication
0.039
ATP
mutant I18W/A23F/C24F, HCO3-dependent ATPase activity, pH 7.6, temperature not specified in the publication
0.043
ATP
mutant M174E/M378E, Gln-dependent ATPase activity, pH 7.6, temperature not specified in the publication
0.053
ATP
mutant M174E, HCO3-dependent ATPase activity, pH 7.6, temperature not specified in the publication
0.06
ATP
-
pH 7.6, 25°C, HCO3(-)-dependent ATPase, mutant D207N
0.07
ATP
-
pH 7.6, 25°C, HCO3(-)-dependent ATPase, mutant F755A
0.072
ATP
mutant G575F, CP-synthesis (co-substrate: L-Gln, HCO3-), pH 7.6, temperature not specified in the publication
0.09
ATP
-
pH 7.6, 25°C, HCO3(-)-dependent ATPase, mutant P690Q
0.09
ATP
-
pH 7.6, 25°C, HCO3(-)-dependent ATPase, wild-type enzyme
0.097
ATP
mutant A23F, HCO3-dependent ATPase activity, pH 7.6, temperature not specified in the publication
0.1
ATP
-
pH 7.6, 25°C, HCO3(-)-dependent ATPase, mutant A144Q
0.11
ATP
-
pH 7.6, 25°C, HCO3(-)-dependent ATPase, mutant D207A
0.11
ATP
mutant M174E, Gln-dependent ATPase activity, pH 7.6, temperature not specified in the publication
0.12
ATP
-
pH 7.6, 25°C, HCO3(-)-dependent ATPase, mutant A144Q/D207A
0.13
ATP
-
pH 7.6, 25°C, HCO3(-)-dependent ATPase, mutant D753N
0.14
ATP
-
pH 7.6, 25°C, HCO3(-)-dependent ATPase, mutant D753A
0.16
ATP
mutant A23K, CP-synthesis (co-substrate: L-Gln, HCO3-), pH 7.6, temperature not specified in the publication
0.17
ATP
mutant A23K, HCO3-dependent ATPase activity, pH 7.6, temperature not specified in the publication
0.17
ATP
wild-type, HCO3-dependent ATPase activity, pH 7.6, temperature not specified in the publication
0.18
ATP
mutant G575K, HCO3-dependent ATPase activity, pH 7.6, temperature not specified in the publication
0.19
ATP
mutant G575F, HCO3-dependent ATPase activity, pH 7.6, temperature not specified in the publication
0.28
ATP
mutant A23K, Gln-dependent ATPase activity, pH 7.6, temperature not specified in the publication
0.29
ATP
-
pH 7.6, 25°C, HCO3(-)-dependent ATPase, mutant I211S
0.33
ATP
mutant M911E, HCO3-dependent ATPase activity, pH 7.6, temperature not specified in the publication
0.35
ATP
-
pH 7.6, 25°C, HCO3(-)-dependent ATPase, mutant S209A
0.39
ATP
mutant L720E, HCO3-dependent ATPase activity, pH 7.6, temperature not specified in the publication
0.5
ATP
mutant M911E, Gln-dependent ATPase activity, pH 7.6, temperature not specified in the publication
0.59
ATP
mutant L648E, HCO3-dependent ATPase activity, pH 7.6, temperature not specified in the publication
1.3
ATP
mutant A23F, Gln-dependent ATPase activity, pH 7.6, temperature not specified in the publication
1.33
ATP
-
pH 7.6, 25°C, mutant D334A, L-glutamine hydrolysis
1.51
ATP
-
pH 7.6, 25°C, Gln-dependent ATPase, mutant D207A
1.65
ATP
-
pH 7.6, 25°C, Gln-dependent ATPase, mutant D207N
1.7
ATP
-
pH 7.6, 25°C, Gln-dependent ATPase, mutant A144Q/D207A
1.71
ATP
-
pH 7.6, 25°C, Gln-dependent ATPase, mutant D753A
1.9
ATP
mutant G575K, CP-synthesis (co-substrate: L-Gln, HCO3-), pH 7.6, temperature not specified in the publication
1.9
ATP
mutant M174E, CP-synthesis (co-substrate: L-Gln, HCO3-), pH 7.6, temperature not specified in the publication
1.9
ATP
wild-type, CP-synthesis (co-substrate: L-Gln, HCO3-), pH 7.6, temperature not specified in the publication
2
ATP
mutant L720E, Gln-dependent ATPase activity, pH 7.6, temperature not specified in the publication
2.13
ATP
-
pH 7.6, 25°C, mutant N311A, L-glutamine hydrolysis
2.2
ATP
-
ammonia-dependent ADP formation
2.43
ATP
-
pH 7.6, 25°C, Gln-dependent ATPase, mutant S209A
3.19
ATP
-
pH 7.6, 25°C, mutant Q351A, L-glutamine hydrolysis
3.2
ATP
mutant L648E, Gln-dependent ATPase activity, pH 7.6, temperature not specified in the publication
3.3
ATP
mutant G575F, Gln-dependent ATPase activity, pH 7.6, temperature not specified in the publication
3.3
ATP
mutant G575K, Gln-dependent ATPase activity, pH 7.6, temperature not specified in the publication
3.35
ATP
-
chimeric enzyme, in comparison to effect of phosphorylation and presence of effectors
3.6
ATP
wild-type, Gln-dependent ATPase activity, pH 7.6, temperature not specified in the publication
3.94
ATP
-
pH 7.6, 25°C, Gln-dependent ATPase, mutant A144Q
4.04
ATP
-
pH 7.6, 25°C, Gln-dependent ATPase, wild-type enzyme
4.77
ATP
-
pH 7.6, 25°C, Gln-dependent ATPase, mutant I211S
4.79
ATP
-
pH 7.6, 25°C, wild-type enzyme, L-glutamine hydrolysis
5.18
ATP
-
pH 7.6, 25°C, Gln-dependent ATPase, mutant F755A
5.3
ATP
-
deletion mutant, in comparison to the presence of effectors
5.6
ATP
-
pH 7.6, 25°C, Gln-dependent ATPase, mutant D753N
5.75
ATP
-
pH 7.6, 25°C, mutant Q310A, L-glutamine hydrolysis
6.8
ATP
-
glutamine-dependent ADP formation
9.01
ATP
-
pH 7.6, 25°C, Gln-dependent ATPase, mutant P690Q
164
ATP
-
pH 7.4, 37°C, mutant hybrid enzyme
2500
ATP
-
pH 7.4, 37°C, wild-type hybrid enzyme
0.1
Gln
-
mutant enzyme E306A, Gln-dependent ATP hydrolysis
0.15
Gln
-
mutant enzyme E25Q/E383Q/E604Q, Gln-dependent ATP hydrolysis
0.17
Gln
-
mutant enzyme E577Q, Gln-dependent ATP hydrolysis
0.32
Gln
-
mutant enzyme E25Q/E383Q, Gln-dependent ATP hydrolysis
0.95
Gln
-
mutant enzyme R848A, Gln-dependent ATP hydrolysis
1.5
Gln
-
mutant enzyme E604Q, Gln-dependent ATP hydrolysis
3.2
Gln
-
mutant enzyme E383Q,Gln-dependent ATP hydrolysis
4
Gln
-
mutant enzyme E383QE916Q, Gln-dependent ATP hydrolysis
4
Gln
-
wild-type enzyme, Gln-dependent ATP hydrolysis
4.2
Gln
-
mutant enzyme E916Q, Gln-dependent ATP hydrolysis
0.005
L-Gln
-
pH 7.6, mutant alphaP360A/alphaH361A/betaR265A
0.1
L-Gln
mutant M174E/M378E, glutaminase activity pH 7.6, temperature not specified in the publication
0.13
L-Gln
mutant I18W/A23F/C24F, glutaminase activity pH 7.6, temperature not specified in the publication
0.13
L-Gln
mutant M378E, glutaminase activity pH 7.6, temperature not specified in the publication
0.14
L-Gln
mutant M911E, glutaminase activity pH 7.6, temperature not specified in the publication
0.15
L-Gln
mutant M174E, glutaminase activity pH 7.6, temperature not specified in the publication
0.21
L-Gln
mutant A23K, glutaminase activity pH 7.6, temperature not specified in the publication
0.84
L-Gln
mutant L720E, glutaminase activity pH 7.6, temperature not specified in the publication
0.88
L-Gln
-
pH 7.6, mutant betaQ262A/betaR265A/betaN266A
1
L-Gln
-
pH 7.6, mutant betaQ262A/betaR265A
1.08
L-Gln
mutant A23F, glutaminase activity pH 7.6, temperature not specified in the publication
1.1
L-Gln
-
pH 7.6, mutant betaR265A
1.2
L-Gln
-
pH 7.6, mutant alphaD362A/betaR265A
1.3
L-Gln
mutant L648E, glutaminase activity pH 7.6, temperature not specified in the publication
1.4
L-Gln
-
pH 7.6, wild-type enzyme
2.2
L-Gln
wild-type, glutaminase activity pH 7.6, temperature not specified in the publication
2.3
L-Gln
-
pH 7.6, mutant alphaP360A/alphaH361A
2.4
L-Gln
-
pH 7.6, mutant alphaD362A
3
L-Gln
mutant G575F, glutaminase activity pH 7.6, temperature not specified in the publication
3.1
L-Gln
mutant G575K, glutaminase activity pH 7.6, temperature not specified in the publication
0.53
L-glutamine
-
pH 7.6, 25°C, mutant Q351A, L-glutamine hydrolysis
1.05
L-glutamine
-
pH 7.6, 25°C, mutant N311A, L-glutamine hydrolysis
1.17
L-glutamine
-
pH 7.6, 25°C, mutant D334A, L-glutamine hydrolysis
1.6
L-glutamine
-
pH 7.6, 25°C, mutant Q310A, L-glutamine hydrolysis
2.13
L-glutamine
-
pH 7.6, 25°C, wild-type enzyme, L-glutamine hydrolysis
193
L-glutamine
-
pH 7.4, 37°C, mutant hybrid enzyme
2660
L-glutamine
-
pH 7.4, 37°C, wild-type hybrid enzyme
0.097
NH4+
-
mutant enzyme E25Q/E383Q/E604Q, NH4Cl-dependent ATP hydrolysis
0.12
NH4+
-
mutant enzyme E577Q, NH4Cl-dependent ATP hydrolysis
0.51
NH4+
-
mutant enzyme E25Q/E383Q, NH4Cl-dependent ATP hydrolysis
1.3
NH4+
-
mutant enzyme E604Q, NH4Cl-depebndent ATP hydrolysis
1.3
NH4+
-
mutant enzyme E916Q, NH4Cl-dependent ATP hydrolysis
1.7
NH4+
-
mutant enzyme E383Q, NH4Cl-dependent ATP hydrolysis
1.9
NH4+
-
pH 7.6, wild-type enzyme
2
NH4+
-
pH 7.6, mutant alphaP360A/alphaH361A
2.5
NH4+
-
pH 7.6, mutant alphaP360A/alphaH361A/betaR265A
2.5
NH4+
-
pH 7.6, mutant betaR265A
3.6
NH4+
-
pH 7.6, mutant betaQ262A/betaR265A
3.8
NH4+
-
pH 7.6, mutant alphaD362A
3.9
NH4+
-
wild-type enzyme, NH4Cl-dependent ATP hydrolysis
5.8
NH4+
-
pH 7.6, mutant alphaD362A/betaR265A
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
comparison of kcat in absence and presence of L-ornithine, UMP and IMP for wild-type and mutant enzymes
-
additional information
additional information
-
comparison of kcat of partial reactions of wild-type and mutant enzymes
-
additional information
additional information
-
comparison of kcat-values for wild-type and mutant enzymes in absence and presence of ornithine
-
additional information
additional information
-
kcat values for the formation of ADP, glutamate and carbonyl phosphate of the wild-type and mutant enzymes, kcat values for the hydrolysis of gamma-glutamyl hydroxamate, gamma-glutamyl hydrazide, gamma-glutamyl hydroxamate and hydroxylamine of the wild-type and mutant enzymes
-