6.3.5.5: carbamoyl-phosphate synthase (glutamine-hydrolysing)
This is an abbreviated version!
For detailed information about carbamoyl-phosphate synthase (glutamine-hydrolysing), go to the full flat file.
Word Map on EC 6.3.5.5
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6.3.5.5
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pyrimidine
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dihydroorotase
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cpsases
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transcarbamoylase
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2.1.3.2
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ornithine-urea
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2.7.2.9
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3.5.2.3
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acivicin
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caspase-activated
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dhoase
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ureotelic
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atcase
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lungfish
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ammonia-dependent
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carab
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5-phosphoribosyl
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1-pyrophosphate
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protopterus
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n-acetyl-l-glutamate
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drug development
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medicine
- 6.3.5.5
- pyrimidine
- dihydroorotase
- cpsases
-
transcarbamoylase
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2.1.3.2
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ornithine-urea
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2.7.2.9
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3.5.2.3
- acivicin
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caspase-activated
- dhoase
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ureotelic
- atcase
- lungfish
-
ammonia-dependent
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carab
-
5-phosphoribosyl
- 1-pyrophosphate
- protopterus
- n-acetyl-l-glutamate
- drug development
- medicine
Reaction
2 ATP
+
Synonyms
CAD, CAD carbamoyl-phosphate synthetase, CAD protein, Carbamoyl phosphate synthase (glutamine), carbamoyl phosphate synthetase, Carbamoyl phosphate synthetase (glutamine-hydrolyzing), carbamoyl phosphate synthetase 1, carbamoyl phosphate synthetase II, carbamoyl phosphate synthetase III, carbamoyl-phosphate synthetase, Carbamoyl-phosphate synthetase (glutamine-hydrolysing), carbamoyl-phosphate synthetase 2, Carbamoylphosphate synthase, Carbamoylphosphate synthetase, Carbamoylphosphate synthetase II, Carbamyl phosphate synthetase (glutamine), Carbamyl phosphate sythetase II, carbamylphosphate synthetase - aspartate transcarbamylase, CPA2, CPS, CPS II (glutamine-dependent), CPS III, CPS III (glutamine- and N-acetyl-L-glutamine-dependent), CPS1, CPSase, CPSase type II, CPSase-A, CPSase-P, CPSII, EC 2.7.2.9, Glutamine-dependent carbamyl phosphate synthetase, glutamine-hydrolyzing CPSase, MGG_04503, More, MtCPSs1, MtCPSs2, Synthase, carbamoylphosphate (glutamine), Synthetase, carbamoylphosphate (glutamine-hydrolyzing)
ECTree
6.3.5.5 carbamoyl-phosphate synthase (glutamine-hydrolysing)Advanced search results
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results (Inhibitors
Inhibitors on EC 6.3.5.5 - carbamoyl-phosphate synthase (glutamine-hydrolysing)
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INHIBITOR 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
acivicin
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selective time-dependent inhibition of L-Gln-dependent activity, L-Gln protects the enzyme from inactivation. Stimulation of NH4+-dependent activity
Ala
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inhibits glutamine hydrolysis and Gln-dependent carbamoyl phosphate synthesis
Alkyl hydrazines
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inhibits Gln-dependent activity, but not NH4+-dependent activity
Arg
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arginine-specific carbamoyl-phosphate synthase is repressed, pyrimidine-specific enzyme not
H2O2
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0.2 mM, inhibits Gln-dependent activity. No effect on the activity with NH4+ in carbamoyl-phosphate synthase reaction
L-2-Amino-4-oxo-5-chloropentanoate
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selective inactivation of Gln-dependent activity
MgUTP2-
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in presence of high concentrations of dimethyl sulfoxide, MgUTP2- at low concentrations stimulates, while it is inhibitory at higher concentrations
N-(phosphonacetyl)-L-aspartate
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there is no preferential partitioning of carbamoyl phosphate between the arginine and pyrimidine biosynthetic pathways. Channeling must occur during the dynamic association of coupled enzymes pairs. The interaction of carbamoyl-phosphate synthetase/aspartate transcarbamoylase is demonstrated by the unexpectedly weak inhibition of the coupled reaction by the bisubstrate analog, N-(phosphonacetyl)-L-aspartate
NEM
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irreversible inactivation of synthetase activity. Increase of glutaminase activity
P1,P5-di(adenosine 5')-pentaphosphate
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inhibits two partial reactions catalyzed by the enzyme: bicarbonate-dependent ATPase and ATP synthesis from carbamoyl phosphate
potassium cyanate
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inhibits Gln-dependent activity, but not NH4+-dependent activity
hydroxylamine
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inhibits Gln-dependent activity, but not NH4+-dependent activity; the inhibitory effect is much greater at pH 9 than at pH 6
hydroxylamine
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inhibits Gln-dependent activity, but not NH4+-dependent activity
polyamines
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inhibition is partially reversed by increasing the concentration of Mg2+ or MgATP2-, or by adding low concentrations of 5-phosphoribosyl 1-diphosphate
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UMP
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the IMP activator and the UMP inhibitor bind to the same site on the enzyme
UMP
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product of the pyrimidine nucleotide pathway, acts as a negative allosteric effector for eCPS, which also catalyzes the first step of this pathway
UMP
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carbamoyl-phosphate synthase belonging to the pyrimidine pathway, carbamoyl-phosphate synthase belonging to the arginine pathway is not inhibited
UMP
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the nonresponsive enzyme can bind UMP at domain D and allosteric effect on chimeric enzyme
UTP
slight UTP inhibition of SU(b) mutant enzyme is probably due to the chelating of Mg2+ by UTP
UTP
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when L-glutamine is the substrate: 94% inhibition of enzyme activity peak 1: CPS II, 6% inhibition of enzyme activity peak 2: CPS III, when NH4Cl is the substrate: 100% inhibition of enzyme activity peak 1: CPS II, 46% inhibition of enzyme activity peak 2: CPS III
UTP
competitive, feedback inhibitor, binds to a regulatory site located in the vicinity of the carbamylphosphate synthetase catalytic subsite
additional information
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IMP binds to enzyme but the actual effect of this ligand is dependent upon temperature and assay conditions
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additional information
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IMP binds to enzyme but the actual effect of this ligand is dependent upon temperature and assay conditions
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additional information
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both the Luc-1b replicon and JFH1-2a virus expression inhibit CAD
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additional information
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acivicin inhibits the growth of Toxoplasma gondii
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