6.3.5.5: carbamoyl-phosphate synthase (glutamine-hydrolysing)
This is an abbreviated version!
For detailed information about carbamoyl-phosphate synthase (glutamine-hydrolysing), go to the full flat file.
Word Map on EC 6.3.5.5
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6.3.5.5
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pyrimidine
-
dihydroorotase
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cpsases
-
transcarbamoylase
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2.1.3.2
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ornithine-urea
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2.7.2.9
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3.5.2.3
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acivicin
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caspase-activated
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dhoase
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ureotelic
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atcase
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lungfish
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ammonia-dependent
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carab
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5-phosphoribosyl
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1-pyrophosphate
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protopterus
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n-acetyl-l-glutamate
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drug development
-
medicine
- 6.3.5.5
- pyrimidine
- dihydroorotase
- cpsases
-
transcarbamoylase
-
2.1.3.2
-
ornithine-urea
-
2.7.2.9
-
3.5.2.3
- acivicin
-
caspase-activated
- dhoase
-
ureotelic
- atcase
- lungfish
-
ammonia-dependent
-
carab
-
5-phosphoribosyl
- 1-pyrophosphate
- protopterus
- n-acetyl-l-glutamate
- drug development
- medicine
Reaction
2 ATP
+
Synonyms
CAD, CAD carbamoyl-phosphate synthetase, CAD protein, Carbamoyl phosphate synthase (glutamine), carbamoyl phosphate synthetase, Carbamoyl phosphate synthetase (glutamine-hydrolyzing), carbamoyl phosphate synthetase 1, carbamoyl phosphate synthetase II, carbamoyl phosphate synthetase III, carbamoyl-phosphate synthetase, Carbamoyl-phosphate synthetase (glutamine-hydrolysing), carbamoyl-phosphate synthetase 2, Carbamoylphosphate synthase, Carbamoylphosphate synthetase, Carbamoylphosphate synthetase II, Carbamyl phosphate synthetase (glutamine), Carbamyl phosphate sythetase II, carbamylphosphate synthetase - aspartate transcarbamylase, CPA2, CPS, CPS II (glutamine-dependent), CPS III, CPS III (glutamine- and N-acetyl-L-glutamine-dependent), CPS1, CPSase, CPSase type II, CPSase-A, CPSase-P, CPSII, EC 2.7.2.9, Glutamine-dependent carbamyl phosphate synthetase, glutamine-hydrolyzing CPSase, MGG_04503, More, MtCPSs1, MtCPSs2, Synthase, carbamoylphosphate (glutamine), Synthetase, carbamoylphosphate (glutamine-hydrolyzing)
ECTree
6.3.5.5 carbamoyl-phosphate synthase (glutamine-hydrolysing)Advanced search results
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results (KM Value
KM Value on EC 6.3.5.5 - carbamoyl-phosphate synthase (glutamine-hydrolysing)
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.022
ADP
mutant A23K, ATP-synthesis, pH 7.6, temperature not specified in the publication
0.03
ADP
mutant G575F, ATP-synthesis, pH 7.6, temperature not specified in the publication
0.054
ADP
mutant G575K, ATP-synthesis, pH 7.6, temperature not specified in the publication
0.088
ADP
mutant M174E, ATP-synthesis, pH 7.6, temperature not specified in the publication
0.13
ADP
mutant M378E, ATP-synthesis, pH 7.6, temperature not specified in the publication
0.13
ADP
wild-type, ATP-synthesis, pH 7.6, temperature not specified in the publication
0.17
ADP
mutant M174E/M378E, ATP-synthesis, pH 7.6, temperature not specified in the publication
0.18
ADP
mutant I18W/A23F/C24F, ATP-synthesis, pH 7.6, temperature not specified in the publication
0.34
ADP
mutant L648E, ATP-synthesis, pH 7.6, temperature not specified in the publication
0.42
ADP
mutant L720E, ATP-synthesis, pH 7.6, temperature not specified in the publication
0.53
ADP
mutant A23F, ATP-synthesis, pH 7.6, temperature not specified in the publication
0.013
ATP
mutant G575K, HCO3-dependent ATPase activity, pH 7.6, temperature not specified in the publication
0.014
ATP
mutant G575F, Gln-dependent ATPase activity, pH 7.6, temperature not specified in the publication
0.014
ATP
mutant G575F, HCO3-dependent ATPase activity, pH 7.6, temperature not specified in the publication
0.03
ATP
mutant M378E, HCO3-dependent ATPase activity, pH 7.6, temperature not specified in the publication
0.038
ATP
-
pH 7.6, wild-type enzyme in presence of ornithine and in comparison to mutant enzymes
0.051
ATP
wild-type, HCO3-dependent ATPase activity, pH 7.6, temperature not specified in the publication
0.07
ATP
mutant G575K, Gln-dependent ATPase activity, pH 7.6, temperature not specified in the publication
0.12
ATP
mutant A23F, HCO3-dependent ATPase activity, pH 7.6, temperature not specified in the publication
0.16
ATP
mutant M378E, Gln-dependent ATPase activity, pH 7.6, temperature not specified in the publication
0.21
ATP
mutant I18W/A23F/C24F, HCO3-dependent ATPase activity, pH 7.6, temperature not specified in the publication
0.22
ATP
mutant L720E, HCO3-dependent ATPase activity, pH 7.6, temperature not specified in the publication
0.24
ATP
wild-type, Gln-dependent ATPase activity, pH 7.6, temperature not specified in the publication
0.25
ATP
mutant I18W/A23F/C24F, Gln-dependent ATPase activity, pH 7.6, temperature not specified in the publication
0.26
ATP
mutant M911E, HCO3-dependent ATPase activity, pH 7.6, temperature not specified in the publication
0.28
ATP
mutant M174E/M378E, Gln-dependent ATPase activity, pH 7.6, temperature not specified in the publication
0.29
ATP
mutant M174E/M378E, HCO3-dependent ATPase activity, pH 7.6, temperature not specified in the publication
0.33
ATP
mutant A23K, HCO3-dependent ATPase activity, pH 7.6, temperature not specified in the publication
0.36
ATP
mutant M174E, HCO3-dependent ATPase activity, pH 7.6, temperature not specified in the publication
0.37
ATP
-
pH 7.6, wild-type enzyme in presence of IMP and in comparison to mutant enzymes
0.38
ATP
mutant A23K, Gln-dependent ATPase activity, pH 7.6, temperature not specified in the publication
0.46
ATP
-
chimeric enzyme, in comparison to effect of phosphorylation and presence of effectors
0.47
ATP
-
pH 7.6, wild-type enzyme in absence of effectors and in comparison to mutant enzymes
0.5
ATP
mutant L648E, HCO3-dependent ATPase activity, pH 7.6, temperature not specified in the publication
0.69
ATP
mutant M174E, Gln-dependent ATPase activity, pH 7.6, temperature not specified in the publication
1
ATP
mutant M911E, Gln-dependent ATPase activity, pH 7.6, temperature not specified in the publication
1.2
ATP
mutant L648E, Gln-dependent ATPase activity, pH 7.6, temperature not specified in the publication
1.6
ATP
-
pH 7.6, wild-type enzyme in presence of UMP and in comparison to mutant enzymes
1.6
ATP
mutant A23F, Gln-dependent ATPase activity, pH 7.6, temperature not specified in the publication
1.7
ATP
-
HCO3-, at 2.0% dimethyl sulfoxide plus 0.3% glycerol or 8.0% glycerol
1.7
ATP
mutant L720E, Gln-dependent ATPase activity, pH 7.6, temperature not specified in the publication
1.2
HCO3-
-
pH 7.5, 37°C, value of wild-type enzyme in comparison to values of mutant enzymes
0.1
L-Gln
mutant A23K, glutaminase activity pH 7.6, temperature not specified in the publication
0.15
L-Gln
wild-type, glutaminase activity pH 7.6, temperature not specified in the publication
0.16
L-Gln
mutant I18W/A23F/C24F, glutaminase activity pH 7.6, temperature not specified in the publication
0.17
L-Gln
mutant G575K, glutaminase activity pH 7.6, temperature not specified in the publication
0.18
L-Gln
mutant L720E, glutaminase activity pH 7.6, temperature not specified in the publication
0.18
L-Gln
mutant M911E, glutaminase activity pH 7.6, temperature not specified in the publication
0.19
L-Gln
mutant G575F, glutaminase activity pH 7.6, temperature not specified in the publication
0.21
L-Gln
mutant A23F, glutaminase activity pH 7.6, temperature not specified in the publication
0.23
L-Gln
mutant L648E, glutaminase activity pH 7.6, temperature not specified in the publication
0.35
L-Gln
mutant M378E, glutaminase activity pH 7.6, temperature not specified in the publication
0.39
L-Gln
mutant M174E, glutaminase activity pH 7.6, temperature not specified in the publication
0.47
L-Gln
mutant M174E/M378E, glutaminase activity pH 7.6, temperature not specified in the publication
22
L-Gln
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carbamoyl-phosphate synthase belonging to the arginine pathway
0.12
L-glutamine
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pH 7.5, 37°C, value of wild-type enzyme in comparison to values of mutant enzymes
0.23
L-glutamine
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wild type enzyme, in 50 mM HEPES (pH 7.5), at 37°C
0.26
L-glutamine
-
mutant enzyme T970A, in 50 mM HEPES (pH 7.5), at 37°C
0.28
L-glutamine
-
mutant enzyme T941F, in 50 mM HEPES (pH 7.5), at 37°C
0.3
L-glutamine
-
mutant enzyme K986I, in 50 mM HEPES (pH 7.5), at 37°C
0.361
L-glutamine
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chimeric enzyme, in presence of ornithine and absence of UMP
0.45
L-glutamine
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mutant enzyme T941F/K986I, in 50 mM HEPES (pH 7.5), at 37°C
0.5
L-glutamine
-
mutant enzyme T941F/T970A/K986I, in 50 mM HEPE (pH 7.5), at 37°C
0.89
L-glutamine
-
Escherichia coli enzyme, in presence of ornithine and absence of UMP
1.49
L-glutamine
-
Escherichia coli enzyme, in absence of ornithine and presence of UMP
1.86
L-glutamine
-
chimeric enzyme, in absence of ornithine and presence of UMP
9.8
NH4+
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carbamoyl-phosphate synthase belonging to the pyrimidine pathway
additional information
additional information
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effect of cryoprotectants on the kinetic parameters
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additional information
additional information
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effect of Orn or UMP on Km-values of mutant and wild type enzyme
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additional information
additional information
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kinetic constants of mutant and wild type enzymes
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additional information
additional information
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kinetic constants of mutant and wild type enzymes
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additional information
additional information
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comparison of KM of partial reactions of wild-type and mutant enzymes
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additional information
additional information
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comparison of Km-values for wild-type and mutant enzymes in absence and presence of ornithine
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additional information
additional information
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Km values for the formation of ADP, glutamate and carbonyl phosphate of the wild-type and mutant enzymes, Km values for the hydrolysis of gamma-glutamyl hydroxamate, gamma-glutamyl hydrazide, gamma-glutamyl hydroxamate and hydroxylamine of the wild-type and mutant enzymes
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additional information
additional information
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kinetics of wild-type and mutant enzymes in L-glutamine-dependent and bicarbonate-dependent ATPase activities, overview
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