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6.3.5.5: carbamoyl-phosphate synthase (glutamine-hydrolysing)

This is an abbreviated version!
For detailed information about carbamoyl-phosphate synthase (glutamine-hydrolysing), go to the full flat file.

Word Map on EC 6.3.5.5

Reaction

2 ATP +

L-glutamine
+
hydrogencarbonate
 +
H2O
=
2 ADP
 +
phosphate
 +
L-glutamate
 +
Carbamoyl phosphate

Synonyms

CAD, CAD carbamoyl-phosphate synthetase, CAD protein, Carbamoyl phosphate synthase (glutamine), carbamoyl phosphate synthetase, Carbamoyl phosphate synthetase (glutamine-hydrolyzing), carbamoyl phosphate synthetase 1, carbamoyl phosphate synthetase II, carbamoyl phosphate synthetase III, carbamoyl-phosphate synthetase, Carbamoyl-phosphate synthetase (glutamine-hydrolysing), carbamoyl-phosphate synthetase 2, Carbamoylphosphate synthase, Carbamoylphosphate synthetase, Carbamoylphosphate synthetase II, Carbamyl phosphate synthetase (glutamine), Carbamyl phosphate sythetase II, carbamylphosphate synthetase - aspartate transcarbamylase, CPA2, CPS, CPS II (glutamine-dependent), CPS III, CPS III (glutamine- and N-acetyl-L-glutamine-dependent), CPS1, CPSase, CPSase type II, CPSase-A, CPSase-P, CPSII, EC 2.7.2.9, Glutamine-dependent carbamyl phosphate synthetase, glutamine-hydrolyzing CPSase, MGG_04503, More, MtCPSs1, MtCPSs2, Synthase, carbamoylphosphate (glutamine), Synthetase, carbamoylphosphate (glutamine-hydrolyzing)

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.5 Carbon-nitrogen ligases with glutamine as amido-N-donor
                6.3.5.5 carbamoyl-phosphate synthase (glutamine-hydrolysing)

Molecular Weight

Molecular Weight on EC 6.3.5.5 - carbamoyl-phosphate synthase (glutamine-hydrolysing)

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
110000
-
1 * 45000, beta, + 1 * 110000, alpha
116200
the large subunit
117700
-
1 * 41400 + 1 * 117700, the large subunit catalyses the carbamoyl phosphate synthesis from ammonia in three steps, and binds the effectors in its 15000 domain
118000
120000
12200
-
1 * 44000 + 1 * 12200, SDS-PAGE
130000
-
1 * 40000 + 1 * 130000, SDS-PAGE
133000
-
1 * 40000, subunit contains the binding site for glutamine, + 1 * 133000, subunit contains the binding sites for NH4+, HCO3-, ATP, and the allosteric effectors. The light and heavy subunits are encoded by the genetically linked car A and car B genes, respectively
160000 - 170000
-
gel filtration, CPS III
163000
-
sedimentation equilibrium ultracentrifugation
168000
-
sucrose density gradient ultracentrifugation
40000
41400
-
1 * 41400 + 1 * 117700, the large subunit catalyses the carbamoyl phosphate synthesis from ammonia in three steps, and binds the effectors in its 15000 domain
42000
44000
-
1 * 44000 + 1 * 12200, SDS-PAGE
45000
56000
-
x * 56000 (subunit N) + x * 71000 (subunit C), SDS-PAGE
58000
-
2 * 58000, SDS-PAGE, chimeric protein
63000
-
CPS.A-subunit, CPS.B-subunit and glutaminase-subunit, 1* 64000 Da, 1 * 63000 Da and 1 * 45000 Da, but only the CPS.A-CPS.B-dimer has catalytic activity, SDS-PAGE
71000
-
x * 56000 (subunit N) + x * 71000 (subunit C), SDS-PAGE
additional information
-
the multienzyme complex of carbamoyl-phosphate synthase(glutamine), aspartate carbamoyltransferase and dihydroorotase has a MW of 870000 as determined by sedimentation equilibrium studies and 210000 by SDS-PAGE