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6.3.5.5: carbamoyl-phosphate synthase (glutamine-hydrolysing)

This is an abbreviated version!
For detailed information about carbamoyl-phosphate synthase (glutamine-hydrolysing), go to the full flat file.

Word Map on EC 6.3.5.5

Reaction

2 ATP +

L-glutamine
+
hydrogencarbonate
 +
H2O
=
2 ADP
 +
phosphate
 +
L-glutamate
 +
Carbamoyl phosphate

Synonyms

CAD, CAD carbamoyl-phosphate synthetase, CAD protein, Carbamoyl phosphate synthase (glutamine), carbamoyl phosphate synthetase, Carbamoyl phosphate synthetase (glutamine-hydrolyzing), carbamoyl phosphate synthetase 1, carbamoyl phosphate synthetase II, carbamoyl phosphate synthetase III, carbamoyl-phosphate synthetase, Carbamoyl-phosphate synthetase (glutamine-hydrolysing), carbamoyl-phosphate synthetase 2, Carbamoylphosphate synthase, Carbamoylphosphate synthetase, Carbamoylphosphate synthetase II, Carbamyl phosphate synthetase (glutamine), Carbamyl phosphate sythetase II, carbamylphosphate synthetase - aspartate transcarbamylase, CPA2, CPS, CPS II (glutamine-dependent), CPS III, CPS III (glutamine- and N-acetyl-L-glutamine-dependent), CPS1, CPSase, CPSase type II, CPSase-A, CPSase-P, CPSII, EC 2.7.2.9, Glutamine-dependent carbamyl phosphate synthetase, glutamine-hydrolyzing CPSase, MGG_04503, More, MtCPSs1, MtCPSs2, Synthase, carbamoylphosphate (glutamine), Synthetase, carbamoylphosphate (glutamine-hydrolyzing)

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.5 Carbon-nitrogen ligases with glutamine as amido-N-donor
                6.3.5.5 carbamoyl-phosphate synthase (glutamine-hydrolysing)

Cloned

Cloned on EC 6.3.5.5 - carbamoyl-phosphate synthase (glutamine-hydrolysing)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
carbamoyl-phosphate synthase belonging to the pyrimidine pathway
-
cloning and characterization of the carAB genes
cloning of car B wild-type and mutant enzymes
-
construction and expression of a functional minigene of CPSII for functional complementation of mutants, overview
-
Escherichia coli and chimeric enzyme are expressed in Escherichia coli L673
-
expressed in Escherichia coli
expressed in Escherichia coli BL21 (DE3) cells
-
expression in Echerichia coli
-
expression in Escherichia coli
expression in Saccharomyces pombe
-
expression of mutant enzymes in Escherichia coli strain RC50
-
expression of wild-type and mutant enzymes
-
expression of wild-type and mutant enzymes in Escherichia coli strain L673
-
gene carB, DNA and amino acid sequence determination and analysis
multifunctional protein, that catalyzes the first three steps in de novo pyrimidine biosynthesis, expression in Escherichia coli
-
pyrAb encodes the large subunit
structure and organization of enzyme gene
-
subcloning of each of the subunits into the pET-28b vector, expression in Escherichia coli
-
the plasmids containing the carAB genes are transformed in the RC50 cell line of Escherichia coli for expression of the wild-type and mutant forms of CPS
-
the two subdomains CPS.A. and CPS.B.
-