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4.4.1.20: leukotriene-C4 synthase

This is an abbreviated version!
For detailed information about leukotriene-C4 synthase, go to the full flat file.

Word Map on EC 4.4.1.20

Reaction

leukotriene C4
=
leukotriene A4
 +
glutathione

Synonyms

(7E,9E,11Z,14Z)-(5S,6S)-5,6-epoxyicosa-7,9,11,14-tetraenoate:glutathione leukotriene-transferase (epoxide-ring-opening), EC 2.5.1.37, leukotriene A4:glutathione S-leukotrienyltransferase, leukotriene C4 synthase, leukotriene C4 synthetase, LT C4 synthase, LTC4 synthase, LTC4 synthetase, LTC4S, LTCS, synthase, leukotriene C4

ECTree

     4 Lyases
         4.4 Carbon-sulfur lyases
             4.4.1 Carbon-sulfur lyases (only sub-subclass identified to date)
                4.4.1.20 leukotriene-C4 synthase

Engineering

Engineering on EC 4.4.1.20 - leukotriene-C4 synthase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A444C
-
the mutation is associated with the risk of ischemic cerebrovascular disease
A52S
-
mutation increases the Km-value for the recombinant enzyme for glutathione
C56S/C82
-
mutant enzyme without altered function
C82V
-
mutant enzyme without altered function
E4K
causes allergic diseases in patients such as bronchial asthma or allergic dermatitis
G1072A
-
the mutation is associated with the risk of ischemic cerebrovascular disease
L121M
by site-directed mutagenesis to prepare the selenomethionine-substituted LTC4S, the mutant yeast clone is grown with selenomethionine, purified and crystallized similarly to the wild-type enzyme
LTCS(1-115)
C-terminally truncated protein, gives a bioluminescence resonance energy transfer signal when fused to Renilla luciferase
LTCS(1-24)
C-terminally truncated protein, gives no bioluminescence resonance energy transfer signal when fused to Renilla luciferase
LTCS(1-58)
C-terminally truncated protein, gives no bioluminescence resonance energy transfer signal when fused to Renilla luciferase
LTCS(1-88)
C-terminally truncated protein, gives a bioluminescence resonance energy transfer signal when fused to Renilla luciferase
LTCS(114-150)
N-terminally truncated protein, gives a bioluminescence resonance energy transfer signal when fused to Renilla luciferase
LTCS(23-115)
C- and N-terminally truncated protein, gives a bioluminescence resonance energy transfer signal when fused to Renilla luciferase
LTCS(23-150)
N-terminally truncated protein, gives a bioluminescence resonance energy transfer signal when fused to Renilla luciferase
LTCS(57-150)
N-terminally truncated protein, gives a bioluminescence resonance energy transfer signal when fused to Renilla luciferase
LTCS(87-150)
N-terminally truncated protein, gives a bioluminescence resonance energy transfer signal when fused to Renilla luciferase
N55A
-
mutation increases the Km-value for the recombinant enzyme for glutathione
R104A
R104K
0.1% of wild-type activity
R104Q
substrate leukotriene A4, 2% of wild-type activity. No activity with substrate leukotriene A4 methyl ester
R104S
4.6% of wild-type activity
R104T
1.1% of wild-type activity
R113A
substrate leukotriene A4, 140% of wild-type activity
R113Q
substrate leukotriene A4, 150% of wild-type activity
R31E
substrate leukotriene A4, 3% of wild-type activity
R31L
substrate leukotriene A4, 3% of wild-type activity
R51A
57% of wild-type activity
R51H
-
fully active mutant enzyme
R51K
-
fully active mutant enzyme
R51Q
29% of wild-type activity
R51T
-
mutant enzyme without activity
R90A
substrate leukotriene A4, 6% of wild-type activity, substrate leukotriene A4 methyl ester, 21% ot wild-type activity
R90Q
substrate leukotriene A4, about 20% of wild-type activity
R92A
substrate leukotriene A4, about 95% of wild-type activity
R92Q
substrate leukotriene A4, about 105% of wild-type activity
R99A
substrate leukotriene A4, about 100% of wild-type activity
R99Q
substrate leukotriene A4, about 70% of wild-type activity
S36E
phosphomimetic mutant, displays about 20 % of wild-type activity. Mutant shows poor lipid substrate binding
T40E
phosphomimetic mutant, activity is hardly affected
V49F
-
mutation increases the Km-value for the recombinant enzyme for glutathione
W116A
site-directed mutagenesis, altered kinetics compared to the wild-type enzyme
W116F
site-directed mutagenesis, the mutant shows a 3fold increased turnover of leukotriene A4 compared to the wild-type enzyme
Y59F
-
mutation increases the Km-value for the recombinant enzyme for glutathione
Y97F
-
mutation increases the Km-value for the recombinant enzyme for glutathione
additional information