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4.4.1.20: leukotriene-C4 synthase

This is an abbreviated version!
For detailed information about leukotriene-C4 synthase, go to the full flat file.

Word Map on EC 4.4.1.20

Reaction

leukotriene C4
=
leukotriene A4
 +
glutathione

Synonyms

(7E,9E,11Z,14Z)-(5S,6S)-5,6-epoxyicosa-7,9,11,14-tetraenoate:glutathione leukotriene-transferase (epoxide-ring-opening), EC 2.5.1.37, leukotriene A4:glutathione S-leukotrienyltransferase, leukotriene C4 synthase, leukotriene C4 synthetase, LT C4 synthase, LTC4 synthase, LTC4 synthetase, LTC4S, LTCS, synthase, leukotriene C4

ECTree

     4 Lyases
         4.4 Carbon-sulfur lyases
             4.4.1 Carbon-sulfur lyases (only sub-subclass identified to date)
                4.4.1.20 leukotriene-C4 synthase

Cloned

Cloned on EC 4.4.1.20 - leukotriene-C4 synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
COS-7 cells transfected with either human or cloned mouse leukotriene C4 synthase cDNA by DEAE-dextran transfection
expressed as an N-terminal hexa-histidine-tagged fusion protein in Pichia pastoris KM71H cells
-
expressed in COS-7 cells
expressed in Schizosaccharomyces pombe
expression in COS-7 cells
expression in Escherichia coli, construction of an enhanced green fluorescent protein under the control of the mouse leukotriene C4 synthase promoter
expression in Pichia pastoris
expression in rat basophilic leukemia RBL-2H3 cells
expression in Schizosaccharomyces pombe
expression in Spodoptera frugiperda
overexpressed
-
overexpression in fission yeast with a His6 tag at the C terminus
overexpression in the yeast Pichia pastoris with a His6 tag
preparation of pRluc-LTC4S and pGFPLTC4S (full length and truncated forms). Full length and truncated forms of LTC4S excised from the pGFP vector and subcloned in frame with GST in a pGEX vector and transformed into Escherichia coli Y1090. Full length LTC4S subcloned into a pDsRed vector. HEK 293/T cells co-transfected with pRluc-LTC4S and pGFP fusion constructs. COS-7 cells co-transfected with GFP-5-lipoxygenase activating protein and dsRed-LTC4S
recombinant enzyme expression in Pichia pastoris strain KM71H
recombinant expression of wild-type and mutant enzymes in Pichia pastoris strain KM71H
transfection of HEK 293 cell