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4.4.1.20: leukotriene-C4 synthase

This is an abbreviated version!
For detailed information about leukotriene-C4 synthase, go to the full flat file.

Word Map on EC 4.4.1.20

Reaction

leukotriene C4
=
leukotriene A4
 +
glutathione

Synonyms

(7E,9E,11Z,14Z)-(5S,6S)-5,6-epoxyicosa-7,9,11,14-tetraenoate:glutathione leukotriene-transferase (epoxide-ring-opening), EC 2.5.1.37, leukotriene A4:glutathione S-leukotrienyltransferase, leukotriene C4 synthase, leukotriene C4 synthetase, LT C4 synthase, LTC4 synthase, LTC4 synthetase, LTC4S, LTCS, synthase, leukotriene C4

ECTree

     4 Lyases
         4.4 Carbon-sulfur lyases
             4.4.1 Carbon-sulfur lyases (only sub-subclass identified to date)
                4.4.1.20 leukotriene-C4 synthase

Purification

Purification on EC 4.4.1.20 - leukotriene-C4 synthase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
affinity chromatography purification based on specific interaction between leukotriene C4 synthase and microsomal glutathione S-transferase which ocurs in the presence of magnesium ion
-
extraction with a Triton X-100/Triton-DOC mixture, two affinity chromatography steps and gel filtration
fractioning
-
Ni-Sepharose column chromatography, S-hexylglutathione agarose chromatography, and Superdex 200 gel filtration
-
partial
solubilized with sodium deoxycholic acid and DDM, four steps of column chromatography, including affinity chromatography with S-hexyl GSH
the recombinant enzyme from Schizosaccharomyces pombe, to apparent homogeneity
to homogeneity