4.2.2.3: mannuronate-specific alginate lyase

This is an abbreviated version!
For detailed information about mannuronate-specific alginate lyase, go to the full flat file.

Reaction

Synonyms

(poly alpha-l-guluronate) lyase, A1 alginate lyase, A1-II, A1-III, A1-IV', A1m, A1mU, A9mC, A9mL, A9mT, AAlyase, AkAly30, AL2, alg, ALG-5, Alg-A, Alg17C, Alg2A, Alg7D, AlgA, AlgB, AlgE7, AlgI, alginase I, alginate lyase, alginate lyase A, alginate lyase A1-II, alginate lyase A1-II', alginate lyase A1-III, alginate lyase AlyPEEC, alginate lyase Atu3025, alginate lyase B, alginate lyase C, alginate lyase I, alginate lyase VI, alginate lyase1-III, AlgL, ALY, ALY-1, Aly-SJ02, Aly1, Aly2, Aly28, Aly30, Aly32, Aly33, Aly35, Aly7B, AlyA, AlyA1, AlyA2, AlyA3, AlyA5, AlyB, AlyDW11, ALYIII, alyPEEC, AlyPI, AlyPM, AlyQ, AlyV5, AlyVI, Atu3025, AXE80_11190, CL2, EC 4.2.99.4, endo-type alginate lyase, endolytic poly(M) lyase, endolytic polymannuronate lyase, exotype alginate lyase, HdAlex, hdalex-1, HdAly, KJ-2 alginate lyase, LbAly28, lyase AlyA, lyase, alginate, Lysis protein, M block-specific polymannuronate lyase, mannuronate alginate lyase, MJ-3 alginate lyase, More, MY04_2544, NIS_0185, Oal17A, oligoalginate lyase, PL-5 alginate lyase, PM lyase, poly(1,4-beta-D-mannuronide) lyase, poly(beta-D-1,4-mannuronide) lyase, Poly(beta-D-mannuronate) lyase, poly(M) lyase, poly(M)lyase, Poly(mana) alginate lyase, poly(mana)alginate lyase, polymannuronate lyase, polyMG-specific alginate lyase, protein PA1167, SP2, V12B01_24254, V12B01_24259, WP_053404615

ECTree

     4 Lyases

         4.2 Carbon-oxygen lyases

             4.2.2 Acting on polysaccharides

                4.2.2.3 mannuronate-specific alginate lyase

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Results	in table
53	Activating Compound
1671	AA Sequence
86	Application
2	CAS Registry Number
78	Cloned(Commentary)
16	Crystallization (Commentary)
48	Disease/ Diagnostics
1	Expression
33	General Information
3	General Stability
187	Inhibitors
20	kcat/KM [mM/s]
1	Ki Value [mM]
72	KM Value [mM]
77	Localization
213	Metals/Ions
128	Molecular Weight [Da]
138	Natural Substrates/ Products (Substrates)
298	Organism
3	Pathway
41	PDB ID
128	pH Optimum
35	pH Range
29	pH Stability
35	pI Value
8	Posttranslational Modification
164	Protein Variants
89	Purification (Commentary)
61	Reaction
1	Reaction Type
237	Reference
1	Renatured (Commentary)
44	Source Tissue
115	Specific Activity [micromol/min/mg]
6	Storage Stability
574	Substrates and Products (Substrate)
95	Subunits
308	Synonyms
1	Systematic Name
90	Temperature Optimum [°C]
28	Temperature Range [°C]
69	Temperature Stability [°C]
36	Turnover Number [1/s]

Temperature Stability

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Temperature Stability on EC 4.2.2.3 - mannuronate-specific alginate lyase

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TEMPERATURE STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

0 - 40

Flammeovirga sp. MY04

A0A1B1FUW4

24 h, at least 60% residual activity

746840

130

Vibrio sp.

-

1 h, stable

677288

20

Bacillus sp. (in: Bacteria)

-

30 min, 40% loss of activity

34064

20 - 50

3 entries

25

Wenyingzhuangia fucanilytica

A0A1B1Y7V0

24 h, 86% residual activity

749227

30

3 entries

30 - 60

Vibrio sp.

Q53EJ1

1 h, residual activity decreases when temperature goes above 30°C and disappears at 60°C

664733

35

5 entries

37

Pseudoalteromonas sp. SM0524

B2LME8

melting temperature

747844

38

3 entries

40

14 entries

41

Omphalius rusticus

-

temperature at which activity decreases to 50% of the original activity for 20 min incubation is 43°C

703781

43

Haliotis discus hannai

-

temperature at which activity decreases to 50% of the original activity for 20 min incubation is 41°C

703781

45

6 entries

5 - 50

Vibrio sp.

C4TJD2, C4TJD3, C4TJD4

the relative activities of isoform A9mT at 10°C and 2°C are around 45% and 30% of the maximal activity at 30°C, respectively, isoform A9mT shows the highest thermal stability in the presence of NaCl, recombinant isoform A9mT is rapidly inactivated above 30°C when the enzyme is incubated at 5°C to 50°C for 15 min in 100 mM Tris-HCl buffer (pH 7.5) with or without 0.2 M NaCl and the enzyme is rapidly inactivated when incubated at above 40°C in the absence of NaCl

705481

50

14 entries

60

3 entries

67

Nitratiruptor sp. SB155-2

A6Q1E0

30 min, 50% loss of activity

748210

70

3 entries

80

2 entries

90

Vibrio sp.

-

purified native enzyme, 10 min, pH 7.0, 40% activity remians

729470

additional information

2 entries

20 - 50

Agarivorans sp.

B5BSV2

A1m is rapidly inactivated upon incubation at 30-50°C for 15 min in 20 mM glycine-NaOH buffer (pH 9) with or without 0.2 M NaCl. The residual activity after treatment at 30°C with 0.2 M NaCl is 53% of the residual activity without NaCl. The residual activities after treatment at 50°C are 10% of the initial activities after incubation at 30°C with or without NaCl. Activation of the enzyme by NaCl is prominent between 25 and 35°C

703603

20 - 50

Pseudoalteromonas atlantica

-

the enzyme activities at 20 and 50°C decrease to 50% and 30%, respectively

701884

20 - 50

Pseudoalteromonas sp.

-

the residual activity decreases when the temperature goes above 20°C and disappears at 50°C

702993

30

Pseudoalteromonas sp. SM0524

B2LME8

unstable above

747844

30

Pseudomonas sp.

Q597B8

1 h, about 10% loss of activity

682995

30

Sphingomonas sp.

Q75WP3

pH 7.5, 5 min, stable

665371

35

Pseudomonas fluorescens

-

30 min, isoform B 59% residual activity, isoform C 55% residual activity

713799

35

Sphingomonas sp.

Q75WP3

pH 7.5, 5 min, about 10% loss of initial activity

665371

35

Vibrio sp.

-

stable below

679376

35

Vibrio sp.

-

1 h, about 50% loss of activity

677288

35

Wenyingzhuangia fucanilytica

A0A1B1Y7V0

24 h, 76% residual activity

749227

38

Aplysia kurodai

-

half inactivation after 20 min

714770

38

Aplysia kurodai

-

half-life of isozymes AkAly28 and AkAly33 is 20 min

714770

38

Haliotis iris

-

temperature at which activity decreases to 50% of the original activity for 20 min incubation is 38°C

703781

40

Flammeovirga sp. MY04

A0A1B1FUS6

wild-type, 2 h, less than 30% residual activity. Mutant T282N, 8 h, 50% residual activity

747854

40

Isoptericola halotolerans

AB489222

purified enzyme, loss of 40% activity after 180 min

729531

40

Isoptericola halotolerans

A0A346FH09

30 min, 80% residual activity

748532

40

Littorina sp.

-

pH 5.6, 1 h, stable

34074

40

Pseudoalteromonas sp.

-

half-life 41 min

716092

40

Pseudoalteromonas sp.

-

half-life is 41 min

716092

40

Pseudomonas fluorescens

-

isoform A, 30 min, 70% residual activity

713799

40

Pseudomonas sp.

Q597B8

1 h, about 20% loss of activity

682995

40

Sphingomonas sp.

-

stable below, 10 min, pH 7.2

651661

40

Sphingomonas sp.

Q75WP3

pH 7.5, 5 min, about 35% loss of initial activity

665371

40

Stenotrophomonas maltophilia

I6P4V6

purified recombinant His-tagged enzyme, 20 min, stable up to, significant loss of activity above

729058

40

Streptomyces sp.

-

activity is stable up to 40°C for 30 min reaction, and then sharply decreases with further increase in temperature

714484

40

Vibrio sp.

-

1 h, about 70% loss of activity

677288

40

Vibrio sp. W13

A0A0A1DYR7

30 min, about 60% residual activity

747949

45

Microbulbifer sp. ALW1

-

1 h, 68% residual activity

748603

45

Paradendryphiella salina

-

stable below

34063

45

Pseudomonas aeruginosa

-

10 min, 80% loss of activity, protein PA1167

665543

45

Pseudomonas sp. E03

A0A0A1DZ36

stable below

747385

45

Sphingomonas sp.

-

pH 7.2, 10 min, loss f 50% activity, isozyme A1-I

653728

45

Sphingomonas sp.

Q75WP3

pH 7.5, 5 min, about 50% loss of initial activity

665371

50

Flammeovirga sp. NJ-04

A0A1Z2QRE2

30 min, more than 80% residual activity

747722

50

Haliotis discus hannai

-

30 min, 50% residual activity

705483

50

Haliotis discus hannai

-

the activity of recombinant Aly is decreased by 50% after incubation at 50°C for 30 min

705483

50

Isoptericola halotolerans

AB489222

purified enzyme, inactivation after 120 min

729531

50

Littorina brevicula

-

temperature at which activity decreases to 50% of the original activity for 20 min incubation is 50°C

703781

50

Littorina sp.

-

1 h, complete inactivation

34074

50

Pseudoalteromonas sp.

-

half-life 20 min

716092

50

Pseudoalteromonas sp.

-

half-life is 20 min

716092

50

Pseudomonas sp.

Q597B8

1 h, about 40% loss of activity

682995

50

Sphingomonas sp.

Q5DWP2

10 min, 85% loss of activity

665769

50

Sphingomonas sp.

-

pH 7.2, 10 min, loss f 50% activity, isozyme A1-I

653728

50

Sphingomonas sp.

Q75WP3

pH 7.5, 5 min, about 65% loss of initial activity

665371

50

Sphingomonas sp. A1

Q75WP3

stable up to

749289

50

Vibrio sp.

-

1 h, about 90% loss of activity

677288

60

Bacillus sp. (in: Bacteria)

-

30 min, 50% loss of activity

34064

60

Isoptericola halotolerans

AB489222

purified enzyme, inactivation after 90 min

729531

60

Pseudomonas sp.

Q597B8

1 h, about 75% loss of activity

682995

70

Cobetia sp. NAP1

-

1 h, about 80% residual activity

747319

70

Pseudoalteromonas elyakovii

-

enzyme totally inactivated at 70°C within 10 min

695268

70

Streptomyces sp.

-

92% of the activity is lost after incubation at 70°C and pH 7.5 for 20 min

680308

80

Cobetia sp. NAP1

-

15 min, about 30% residual activity

747319

80

Pseudomonas sp.

Q597B8

1 h, complete loss of activity

682995

additional information

Alteromonas sp.

-

-

648533

additional information

Nitratiruptor sp. SB155-2

A6Q1E0

heat stability is decreased in the presence of 5 mM dithiotreitol, and residues Cys80 and Cys232 are responsible for heat stability but not lyase activity

748210