Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) at 37°C and at 15°C. The recombinant alginate lyase expressed with pColdI at 15°C exhibits the highest alginate-degrading activity, method evaluation and optimization | Streptomyces sp. |
Protein Variants | Comment | Organism |
---|---|---|
additional information | recombinant alginate lyase is efficiently immobilized onto two types of magnetic nanoparticles, superparamagnetic iron oxide nanoparticle, and hybrid magnetic silica nanoparticle, based on the affinity between His-tag and Ni2+ displayed on the surfaces of nanoparticles. An alginate oligosaccharide mixture consisting of dimer and trimer is synthesized by the immobilized alginate lyase. The immobilized enzymes can be re-used repeatedly more than 10 times after magnetic separation, overview | Streptomyces sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates | Streptomyces sp. | |
Co2+ | activates | Streptomyces sp. | |
Mg2+ | activates | Streptomyces sp. | |
Mn2+ | activates | Streptomyces sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27500 | - |
x * 27500, recombinant His-tagged enzyme, SDS-PAGE | Streptomyces sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | - |
- |
- |
Streptomyces sp. ALG-5 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Streptomyces sp. |
Subunits | Comment | Organism |
---|---|---|
? | x * 27500, recombinant His-tagged enzyme, SDS-PAGE | Streptomyces sp. |
Synonyms | Comment | Organism |
---|---|---|
alginate lyase | - |
Streptomyces sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Streptomyces sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 80 | - |
Streptomyces sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
activity is stable up to 40°C for 30 min reaction, and then sharply decreases with further increase in temperature | Streptomyces sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Streptomyces sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.6 | 11 | - |
Streptomyces sp. |
General Information | Comment | Organism |
---|---|---|
physiological function | the marine alginate lyase from Streptomyces sp. ALG-5 specifically degrades poly-G block of alginate | Streptomyces sp. |