Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.2.3 extracted from

  • Elyakova, L.A.; Favorov, V.V.
    Isolation and certain properties of alginate lyase VI from the mollusk Littorina sp. (1974), Biochim. Biophys. Acta, 358, 341-354.
No PubMed abstract available
Search for more literature for 4.2.2.3

Inhibitors

Inhibitors Comment Organism Structure
Cd2+
-
 Littorina sp.
Cu2+
-
 Littorina sp.
dodecylsulfate
-
 Littorina sp.
Hg2+
-
 Littorina sp.
PCMB
-
 Littorina sp.
Zn2+
-
 Littorina sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.19
-
 alginate
-
 Littorina sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 1 mM, 55% activation Littorina sp.
Co2+ 1 mM, 80% activation Littorina sp.
Cs+ 1 mM, 40% activation Littorina sp.
K+ 1 mM, 15% activation Littorina sp.
Li+ 1 mM, 12% activation Littorina sp.
Mg2+ 1 mM, 40% activation Littorina sp.
NaN3 1 mM, 20% activation Littorina sp.
NH4+ 1 mM, 12% activation Littorina sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 gel filtration Littorina sp.

Organism

Organism UniProt Comment Textmining
Littorina sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Littorina sp.

Source Tissue

Source Tissue Comment Organism Textmining
hepatopancreas
-
 Littorina sp.
-

Storage Stability

Storage Stability Organism
-20°C, 3 months, stable Littorina sp.
4°C, in presence of sodium azide, 10 days, 10% loss of activity Littorina sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alginate preferential splitting of the glycosidic bond between two mannuronide residues in the alginic acid Littorina sp. oligouronides
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 pH 5.6, 1 h, stable Littorina sp.
50
-
 1 h, complete inactivation Littorina sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6
-
-
 Littorina sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 7 pH 5.0: about 40% of maximal activity, pH 7.0: about 25% of maximal activity Littorina sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 8 stable Littorina sp.
4 10 37°C, 3 h, about 40% loss of activity at pH 4.0, about 30% loss of activity, completely stable at pH 5.0 Littorina sp.