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4.2.2.3: mannuronate-specific alginate lyase

This is an abbreviated version!
For detailed information about mannuronate-specific alginate lyase, go to the full flat file.

Word Map on EC 4.2.2.3

Reaction

R2-beta-D-mannuronic acid-R1
=
R2-OH
 +
4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-R1

Synonyms

(poly alpha-l-guluronate) lyase, A1 alginate lyase, A1-II, A1-III, A1-IV', A1m, A1mU, A9mC, A9mL, A9mT, AAlyase, AkAly30, AL2, alg, ALG-5, Alg-A, Alg17C, Alg2A, Alg7D, AlgA, AlgB, AlgE7, AlgI, alginase I, alginate lyase, alginate lyase A, alginate lyase A1-II, alginate lyase A1-II', alginate lyase A1-III, alginate lyase AlyPEEC, alginate lyase Atu3025, alginate lyase B, alginate lyase C, alginate lyase I, alginate lyase VI, alginate lyase1-III, AlgL, ALY, ALY-1, Aly-SJ02, Aly1, Aly2, Aly28, Aly30, Aly32, Aly33, Aly35, Aly7B, AlyA, AlyA1, AlyA2, AlyA3, AlyA5, AlyB, AlyDW11, ALYIII, alyPEEC, AlyPI, AlyPM, AlyQ, AlyV5, AlyVI, Atu3025, AXE80_11190, CL2, EC 4.2.99.4, endo-type alginate lyase, endolytic poly(M) lyase, endolytic polymannuronate lyase, exotype alginate lyase, HdAlex, hdalex-1, HdAly, KJ-2 alginate lyase, LbAly28, lyase AlyA, lyase, alginate, Lysis protein, M block-specific polymannuronate lyase, mannuronate alginate lyase, MJ-3 alginate lyase, More, MY04_2544, NIS_0185, Oal17A, oligoalginate lyase, PL-5 alginate lyase, PM lyase, poly(1,4-beta-D-mannuronide) lyase, poly(beta-D-1,4-mannuronide) lyase, Poly(beta-D-mannuronate) lyase, poly(M) lyase, poly(M)lyase, Poly(mana) alginate lyase, poly(mana)alginate lyase, polymannuronate lyase, polyMG-specific alginate lyase, protein PA1167, SP2, V12B01_24254, V12B01_24259, WP_053404615

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.2 Acting on polysaccharides
                4.2.2.3 mannuronate-specific alginate lyase

Specific Activity

Specific Activity on EC 4.2.2.3 - mannuronate-specific alginate lyase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.01
substrate poly(alpha-(1,4)-L-guluronate), mutant G118N, pH 7.0, 30°C
0.021
purified recombinant enzyme
0.0274
-
protein PA1167
0.03
substrate poly(alpha-(1,4)-L-guluronate), mutant C115A/C124G, pH 7.0, 30°C
0.08
substrate poly(beta-(1,4)-D-mannuronate/alpha-(1,4)-L-guluronate), mutant G118N, pH 7.0, 30°C
0.11
substrate alginate, mutant Y142F, pH 7.0, 30°C
0.142
crude extract, at pH 10.0
0.16
substrate poly(alpha-(1,4)-L-guluronate), wild-type, pH 7.0, 30°C
0.18
substrate poly(beta-(1,4)-D-mannuronate), mutant G118N, pH 7.0, 30°C
0.22
substrate alginate, mutant Y140F, pH 7.0, 30°C
0.31
substrate alginate, mutant G118N, pH 7.0, 30°C
0.83
substrate poly(beta-(1,4)-D-mannuronate/alpha-(1,4)-L-guluronate), mutant C115A/C124G, pH 7.0, 30°C
1.14
wild-type, pH 7.5, 30°C
1.98
-
wild-type enzyme, pH 7.0, 50°C, substrate alginate
108.5
after 764.1fold purification, at pH 10.0
109
-
purified isozyme A1-II
1123
-
extracellular enzyme, pH 7.0, 30°C
12.31
substrate poly(beta-(1,4)-D-mannuronate), pH 7.0, 30°C
129
-
alginate lyase C, substrate with G blocks, pH 7.0, 30°C
1401
pH 7.5, 30°C
1487.6
substrate poly(beta-(1,4)-D-mannuronate), pH 7.0, 50°C
1620
substrate poly(beta-1,4-D-mannuronate), pH 6.0, 70°C
17.4
substrate alginate, wild-type, pH 7.0, 30°C
1786
pH 7.0, 30°C
2.2
substrate poly(beta-(1,4)-D-mannuronate/alpha-(1,4)-L-guluronate), wild-type, pH 7.0, 30°C
2025
wild-type, pH 6.0, 40°C
2043
Vibrio sp. NJ-04
substrate poly(beta-(1,4)-D-mannuronate), pH 7.0, 40°C
21.14
substrate alginate, pH 7.0, 30°C
21.48
substrate poly(beta-(1,4)-D-mannuronate), pH 8.0, 35°C
21.77
-
alginate lyase C, substrate with M blocks, pH 7.0, 30°C
2100
purified recombinant His-tagged AkAly30, pH 8.0, 30°C
2152
-
purified enzyme, pH 7.0, 38°C
23.34
substrate alginate, pH 8.0, 35°C
2416
Vibrio sp. NJ-04
substrate alginate, pH 7.0, 40°C
2490
-
30°C, pH 8.0
2604.9
substrate poly(alpha-(1,4)-L-guluronate), pH 7.0, 50°C
27.03
substrate poly(beta-(1,4)-D-mannuronate), pH 8.0, 35°C
2920
mutant T282N, pH 6.0, 40°C
33
substrate poly(beta-(1,4)-D-mannuronate), wild-type, pH 7.0, 30°C
330
-
alginate lyase B, substrate with G blocks, pH 7.0, 30°C
3343.7
substrate alginate, pH 7.0, 50°C
3359
Vibrio sp. NJ-04
substrate poly(alpha-(1,4)-L-guluronate), pH 7.0, 40°C
34.4
-
purified enzyme
390
-
alginate lyase A, substrate with G blocks, pH 7.0, 30°C
41.4
-
purified recombinant His-tagged enzyme, pH 7.0, 50°C, substrate alginate
45
-
purified isozyme A1-III
457
pH 8.0, 30°C
467
-
isoform B, substrate sodium alginate, pH 7.0, 30°C
5740
-
pH 7.0, 30°C
5741
-
purified isozyme AkAly28, pH 8.0, 37°C
5796
purified native AkAly30, pH 8.0, 30°C
6
substrate alginate, mutant C115A/C124G, pH 7.0, 30°C
6.8
substrate poly(beta-(1,4)-D-mannuronate), mutant C115A/C124G, pH 7.0, 30°C
653
-
isoform A, substrate sodium alginate, pH 7.0, 30°C
67.24
-
purified enzyme, pH 6.5, 35°C
7.43
substrate poly(alpha-(1,4)-L-guluronate), pH 7.0, 30°C
73.1
-
purified isozyme A1-I
7658
substrate poly(beta-(1,4)-D-mannuronic acid), pH 7.5, 55°C
7985
substrate alginate, pH 7.5, 55°C
8409
AB489222
purified native enzyme, pH 7.0, 50°C
8643
substrate poly(alpha-(1,4)-L-guluronic acid), pH 7.5, 55°C
93.88
purified enzyme
937
-
substrate poly(beta-D-mannuronic acid/alpha-L-guluronic acid), 22°C, pH 7.5
additional information