Application | Comment | Organism |
---|---|---|
synthesis | aly-SJ02 may be a good tool to produce dimers and trimers from alginate | Pseudoalteromonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | slightly inhibiting | Pseudoalteromonas sp. | |
EDTA | 1 mM, 48% of initial activity; 51.7% inhibition at 1 mM | Pseudoalteromonas sp. | |
Sn2+ | slightly inhibiting | Pseudoalteromonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | KM-value for alpha-L-guluronosyl linkage in alginate, 1.086 mg/ml, for poly(alpha-L-guluronic acid), 2.751 mg/ml at pH 8.5, 50°C | Pseudoalteromonas sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Pseudoalteromonas sp. | - |
- |
extracellular | aly-SJ02 is secreted | Pseudoalteromonas sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | activates | Pseudoalteromonas sp. | |
Ba2+ | 1mM, 143% of initial activity | Pseudoalteromonas sp. | |
Ca2+ | activates | Pseudoalteromonas sp. | |
Ca2+ | 1mM, 136% of initial activity | Pseudoalteromonas sp. | |
Co2+ | activates | Pseudoalteromonas sp. | |
Co2+ | 1mM, 123% of initial activity | Pseudoalteromonas sp. | |
K+ | activates | Pseudoalteromonas sp. | |
K+ | 1mM, 117% of initial activity | Pseudoalteromonas sp. | |
Mg2+ | activates | Pseudoalteromonas sp. | |
Mg2+ | 1mM, 126% of initial activity | Pseudoalteromonas sp. | |
Mn2+ | 1mM, 116% of initial activity | Pseudoalteromonas sp. | |
additional information | Zn2+ has no effect on aly-SJ02 activity | Pseudoalteromonas sp. | |
Na+ | activates | Pseudoalteromonas sp. | |
Na+ | 1mM, 143% of initial activity | Pseudoalteromonas sp. | |
NaCl | aly-SJ02 shows the highest activity in 0.2 M NaCl, and retains more than 75% activity in 1 M NaCl, exhibiting salt-tolerance ability | Pseudoalteromonas sp. | |
Ni2+ | activates | Pseudoalteromonas sp. | |
Ni2+ | 1mM, 110% of initial activity | Pseudoalteromonas sp. | |
Sr2+ | activates | Pseudoalteromonas sp. | |
Sr2+ | 1mM, 124% of initial activity | Pseudoalteromonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
x * 32000, SDS-PAGE | Pseudoalteromonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudoalteromonas sp. | alginate is a linear hetero-polyuronic acid composed of 1,4 linked alpha-L-guluronic acid (G) and beta-D-mannuronic acid. Alginate lyase aly-SJ02 is bifunctional. Aly-SJ02 shows activities toward both polyG (alpha-L-guluronic acid), activity of EC 4.2.2.11, and polyM (beta-D-mannuronic acid). Aly-SJ02 mainly releases dimers and trimers from polyM and alginate, and trimers and tetramers from polyG | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudoalteromonas sp. | - |
bifunctional enzyme, actitivies against poly(beta-D-mannuronate), EC 4.2.2.3, and poly(alpha-L-guluronate), EC 4.2.2.11 | - |
Pseudoalteromonas sp. | - |
from marine rotten kelp from a kelp culture field at the seashore of Yantai, China | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudoalteromonas sp. |
native, secreted aly-SJ02 73.4fold by ultrafiltration, anion exchange chromatography, and gel filtration | Pseudoalteromonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4803 | - |
pH 8.5, 50°C | Pseudoalteromonas sp. |
4803 | - |
purified enzyme, pH 8.5, 50°C | Pseudoalteromonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-L-mannuronyl linkage in alginate | - |
Pseudoalteromonas sp. | ? | enzyme shows activities toward both polyG, i.e.alpha-L-guluronic acid, and polyM, i.e. beta-D-mannuronic acid | ? | |
additional information | alginate is a linear hetero-polyuronic acid composed of 1,4 linked alpha-L-guluronic acid (G) and beta-D-mannuronic acid. Alginate lyase aly-SJ02 is bifunctional. Aly-SJ02 shows activities toward both polyG (alpha-L-guluronic acid), activity of EC 4.2.2.11, and polyM (beta-D-mannuronic acid). Aly-SJ02 mainly releases dimers and trimers from polyM and alginate, and trimers and tetramers from polyG | Pseudoalteromonas sp. | ? | - |
? | |
poly(beta-(1->4)-D-mannuronate) | - |
Pseudoalteromonas sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, SDS-PAGE | Pseudoalteromonas sp. |
Synonyms | Comment | Organism |
---|---|---|
alginate lyase | - |
Pseudoalteromonas sp. |
Aly-SJ02 | - |
Pseudoalteromonas sp. |
polymannuronate lyase | - |
Pseudoalteromonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Pseudoalteromonas sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
3 | 65 | assay range | Pseudoalteromonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
half-life 41 min | Pseudoalteromonas sp. |
40 | - |
half-life is 41 min | Pseudoalteromonas sp. |
50 | - |
half-life 20 min | Pseudoalteromonas sp. |
50 | - |
half-life is 20 min | Pseudoalteromonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Pseudoalteromonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | assay range | Pseudoalteromonas sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 11 | at 25 °C for 20 min, the purified enzyme retains more than 50% activity at pH 7.0-10, aly-SJ02 is most stable at pH 8.0 | Pseudoalteromonas sp. |
8 | - |
most stable at pH 8.0 | Pseudoalteromonas sp. |
General Information | Comment | Organism |
---|---|---|
evolution | N-terminal sequence analysis suggest that aly-SJ02 may be an alginate lyase of polysaccharide lyase family 18 | Pseudoalteromonas sp. |