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Literature summary for 4.2.2.3 extracted from

  • Hashimoto, W.; Miyake, O.; Momma, K.; Kawai, S.; Murata, K.
    Molecular identification of oligoalginate lyase of Sphingomonas sp. strain A1 as one of the enzymes required for complete depolymerization of alginate (2000), J. Bacteriol., 182, 4572-4577.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
Ca2+ 1.4-2fold increase in activity of oligoalginate lyase at 1 mM Sphingomonas sp.
Fe2+ 1.4-2fold increase in activity of oligoalginate lyase at 1 mM Sphingomonas sp.
Mg2+ 1.4-2fold increase in activity of oligoalginate lyase at 1 mM Sphingomonas sp.
Mn2+ 1.4-2fold increase in activity of oligoalginate lyase at 1 mM Sphingomonas sp.
additional information oligoalginate lyase is not affected by addition of 1 mM EDTA, GSH, 2-mercaptoethanol, iodoacetic acid, N-ethylmaleimide, and sugars at 5 mM, L-fucose, D-galactose, D-glucose, D-glucuronic acid, D-mannose, L-rhamnose, and D-xylose, no effect on oligoalginate lyase by Cu2+, Co2+, and Hg2+ at 1 mM Sphingomonas sp.

Cloned(Commentary)

Cloned (Comment) Organism
gene encoding the oligoalginate lyase, DNA sequence determination and analysis, genetic mapping Sphingomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
additional information oligoalginate lyase is not affected by addition of 1 mM EDTA, GSH, 2-mercaptoethanol, iodoacetic acid, N-ethylmaleimide, and sugars at 5 mM, L-fucose, D-galactose, D-glucose, D-glucuronic acid, D-mannose, L-rhamnose, and D-xylose, no effect on oligoalginate lyase by Cu2+, Co2+, and Hg2+ at 1 mM Sphingomonas sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Sphingomonas sp. 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
83000
-
oligoalginate lyase, native PAGE Sphingomonas sp.
85000
-
1 * 85000, oligoalginate lyase, SDS-PAGE Sphingomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alginate Sphingomonas sp. incorporated into cells, substrate of alginate lyase isozymes A1-I, A1-II, and A1-III ?
-
?
disaccharides of alginate Sphingomonas sp. oligoalginate lyase, complete depolymerization of alginate monosaccharides of alginate
-
?
trisaccharides of alginate Sphingomonas sp. oligoalginate lyase, complete depolymerization of alginate monosaccharides of alginate
-
?

Organism

Organism UniProt Comment Textmining
Sphingomonas sp.
-
isoforms A1-I, A1-II, and A1-III of alginate lyase, 1 oligoalginate lyase
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification A1-I is autolytically cleaved into A1-II and A1-III, which shows different substrate specificities, therfore A1-I possesses 3 active sites, 1 protease and 2 alginate lyase sites Sphingomonas sp.

Purification (Commentary)

Purification (Comment) Organism
40.8fold Sphingomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Sphingomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alginate substrate of alginate lyase isozymes A1-I, A1-II, and A1-III Sphingomonas sp. ?
-
?
alginate incorporated into cells, substrate of alginate lyase isozymes A1-I, A1-II, and A1-III Sphingomonas sp. ?
-
?
disaccharides of alginate oligoalginate lyase Sphingomonas sp. monosaccharides of alginate
-
?
disaccharides of alginate oligoalginate lyase, complete depolymerization of alginate Sphingomonas sp. monosaccharides of alginate
-
?
additional information oligoalginate lyase substrate specificity Sphingomonas sp. ?
-
?
trisaccharides of alginate oligoalginate lyase Sphingomonas sp. monosaccharides of alginate
-
?
trisaccharides of alginate oligoalginate lyase, complete depolymerization of alginate Sphingomonas sp. monosaccharides of alginate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 85000, oligoalginate lyase, SDS-PAGE Sphingomonas sp.

Synonyms

Synonyms Comment Organism
oligoalginate lyase
-
Sphingomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Sphingomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
stable below, 10 min, pH 7.2 Sphingomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Sphingomonas sp.