Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1.4-2fold increase in activity of oligoalginate lyase at 1 mM | Sphingomonas sp. | |
Fe2+ | 1.4-2fold increase in activity of oligoalginate lyase at 1 mM | Sphingomonas sp. | |
Mg2+ | 1.4-2fold increase in activity of oligoalginate lyase at 1 mM | Sphingomonas sp. | |
Mn2+ | 1.4-2fold increase in activity of oligoalginate lyase at 1 mM | Sphingomonas sp. | |
additional information | oligoalginate lyase is not affected by addition of 1 mM EDTA, GSH, 2-mercaptoethanol, iodoacetic acid, N-ethylmaleimide, and sugars at 5 mM, L-fucose, D-galactose, D-glucose, D-glucuronic acid, D-mannose, L-rhamnose, and D-xylose, no effect on oligoalginate lyase by Cu2+, Co2+, and Hg2+ at 1 mM | Sphingomonas sp. |
Cloned (Comment) | Organism |
---|---|
gene encoding the oligoalginate lyase, DNA sequence determination and analysis, genetic mapping | Sphingomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | oligoalginate lyase is not affected by addition of 1 mM EDTA, GSH, 2-mercaptoethanol, iodoacetic acid, N-ethylmaleimide, and sugars at 5 mM, L-fucose, D-galactose, D-glucose, D-glucuronic acid, D-mannose, L-rhamnose, and D-xylose, no effect on oligoalginate lyase by Cu2+, Co2+, and Hg2+ at 1 mM | Sphingomonas sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Sphingomonas sp. | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
83000 | - |
oligoalginate lyase, native PAGE | Sphingomonas sp. |
85000 | - |
1 * 85000, oligoalginate lyase, SDS-PAGE | Sphingomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alginate | Sphingomonas sp. | incorporated into cells, substrate of alginate lyase isozymes A1-I, A1-II, and A1-III | ? | - |
? | |
disaccharides of alginate | Sphingomonas sp. | oligoalginate lyase, complete depolymerization of alginate | monosaccharides of alginate | - |
? | |
trisaccharides of alginate | Sphingomonas sp. | oligoalginate lyase, complete depolymerization of alginate | monosaccharides of alginate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingomonas sp. | - |
isoforms A1-I, A1-II, and A1-III of alginate lyase, 1 oligoalginate lyase | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | A1-I is autolytically cleaved into A1-II and A1-III, which shows different substrate specificities, therfore A1-I possesses 3 active sites, 1 protease and 2 alginate lyase sites | Sphingomonas sp. |
Purification (Comment) | Organism |
---|---|
40.8fold | Sphingomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Sphingomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alginate | substrate of alginate lyase isozymes A1-I, A1-II, and A1-III | Sphingomonas sp. | ? | - |
? | |
alginate | incorporated into cells, substrate of alginate lyase isozymes A1-I, A1-II, and A1-III | Sphingomonas sp. | ? | - |
? | |
disaccharides of alginate | oligoalginate lyase | Sphingomonas sp. | monosaccharides of alginate | - |
? | |
disaccharides of alginate | oligoalginate lyase, complete depolymerization of alginate | Sphingomonas sp. | monosaccharides of alginate | - |
? | |
additional information | oligoalginate lyase substrate specificity | Sphingomonas sp. | ? | - |
? | |
trisaccharides of alginate | oligoalginate lyase | Sphingomonas sp. | monosaccharides of alginate | - |
? | |
trisaccharides of alginate | oligoalginate lyase, complete depolymerization of alginate | Sphingomonas sp. | monosaccharides of alginate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 85000, oligoalginate lyase, SDS-PAGE | Sphingomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
oligoalginate lyase | - |
Sphingomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Sphingomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
stable below, 10 min, pH 7.2 | Sphingomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Sphingomonas sp. |