Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | enhances activity | Vibrio sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | - |
Vibrio sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Vibrio sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | enhances activity | Vibrio sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62500 | - |
gel filtration | Vibrio sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio sp. | - |
- |
- |
Vibrio sp. YWA | - |
- |
- |
Purification (Comment) | Organism |
---|---|
combination of ammonium sulfate precipitation and diethylaminoethyl-Sephacel column chromatography | Vibrio sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alginate | the enzyme is specific for hydrolyzing poly-beta-D-1,4-mannuronate in alginate | Vibrio sp. | ? | - |
? | |
alginate | the enzyme is specific for hydrolyzing poly-beta-D-1,4-mannuronate in alginate | Vibrio sp. YWA | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Vibrio sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 35 | 15°C: about 70% of maximal activity, 35°C: about 40% of maximal activity | Vibrio sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
1 h, about 50% loss of activity | Vibrio sp. |
40 | - |
1 h, about 70% loss of activity | Vibrio sp. |
50 | - |
1 h, about 90% loss of activity | Vibrio sp. |
130 | - |
1 h, stable | Vibrio sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Vibrio sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | stable | Vibrio sp. |