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Literature summary for 4.2.2.3 extracted from

  • Kobayashi, T.; Uchimura, K.; Miyazaki, M.; Nogi, Y.; Horikoshi, K.
    A new high-alkaline alginate lyase from a deep-sea bacterium Agarivorans sp. (2009), Extremophiles, 13, 121-129.
    View publication on PubMed
Search for more literature for 4.2.2.3

Activating Compound

Activating Compound Comment Organism Structure
(NH4)2SO4 409% relative activity at 200 mM Agarivorans sp.
CH3COO- NH4+ 298% relative activity at 200 mM Agarivorans sp.
CH3COONa 233% relative activity at 200 mM Agarivorans sp.
K2HPO4 439% relative activity at 200 mM Agarivorans sp.
KCl 521% relative activity at 200 mM Agarivorans sp.
NaBr 712% relative activity at 200 mM Agarivorans sp.
NaCl best activator, 823% relative activity at 200 mM, the activity is increased to 1.8times by adding 0.2 M NaCl, the activity reaches maximum at 0.6-0.8 M NaCl, which is around 20 times without NaCl Agarivorans sp.
NaF 419% relative activity at 200 mM Agarivorans sp.
NH4Cl 549% relative activity at 200 mM Agarivorans sp.

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 95% inhibition at 1 mM Agarivorans sp.
CuSO4 7% inhibition at 1 mM Agarivorans sp.
EDTA 86%inhibition at 5 mM Agarivorans sp.
EGTA 86% inhibition at 5 mM Agarivorans sp.
Hg2+ 80% inhibition at 1 mM Agarivorans sp.
Mn2+ 28% inhibition at 1 mM Agarivorans sp.
additional information Ni2+, Co2+, and Mg2+ ions do not affect enzyme activity, dithiothreitol and 2-mercaptoethanol (5 mM each) do not affect the enzyme activity at all Agarivorans sp.
Ni2+ 95% inhibition at 1 mM Agarivorans sp.
Pd2+ 95% inhibition at 1 mM Agarivorans sp.
Sr2+ 46% inhibition at 1 mM Agarivorans sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ 111% relative activity at 1 mM Agarivorans sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 SDS-PAGE Agarivorans sp.
32300
-
 calculated from amino acid sequence Agarivorans sp.

Organism

Organism UniProt Comment Textmining
Agarivorans sp. B5BSV2
-
-
Agarivorans sp. JAM-A1m B5BSV2
-
-

Purification (Commentary)

Purification (Comment) Organism
SuperQ Toyopearl column chromatography, butyl Toyopearl column chromatography, and ammonium sulfate fractionation Agarivorans sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.142
-
 crude extract, at pH 10.0 Agarivorans sp.
108.5
-
 after 764.1fold purification, at pH 10.0 Agarivorans sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alginate the enzyme degrades alginate by endo fashion, the enzyme degrades favorably mannuronate-guluronate and guluronate-rich fragments in alginate, shows substrate specificity toward beta-D-mannuronic acid blocks of hydrolysed alginate Agarivorans sp. unsaturated algino-oligosaccharides 4-deoxy-L-erythro-hex-4-enopyranosyluronic acids as the non-reducing terminal moiety are contained in the products ?
alginate the enzyme degrades alginate by endo fashion, the enzyme degrades favorably mannuronate-guluronate and guluronate-rich fragments in alginate, shows substrate specificity toward beta-D-mannuronic acid blocks of hydrolysed alginate Agarivorans sp. JAM-A1m unsaturated algino-oligosaccharides 4-deoxy-L-erythro-hex-4-enopyranosyluronic acids as the non-reducing terminal moiety are contained in the products ?

Synonyms

Synonyms Comment Organism
A1m
-
 Agarivorans sp.
alginate lyase
-
 Agarivorans sp.
polymannuronate lyase
-
 Agarivorans sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 around 30°C Agarivorans sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 50 A1m is rapidly inactivated upon incubation at 30-50°C for 15 min in 20 mM glycine-NaOH buffer (pH 9) with or without 0.2 M NaCl. The residual activity after treatment at 30°C with 0.2 M NaCl is 53% of the residual activity without NaCl. The residual activities after treatment at 50°C are 10% of the initial activities after incubation at 30°C with or without NaCl. Activation of the enzyme by NaCl is prominent between 25 and 35°C Agarivorans sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
 the optimal pH is around 10 in glycine-NaOH buffer Agarivorans sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10 A1m is quite stable in incubation at 30°C for 1 h between pH 6.0 and 9.0. When the optimal pH in the presence of 0.2 M NaCl is shifted to pH 9.0, the activity is more than 10times compared with that at pH 9 in the absence of NaCl. Similar results are observed in 100 mM borate buffer with or without 0.2 M NaCl. The stability of A1m in various pH buffers is unaffected by adding NaCl Agarivorans sp.