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4.2.2.3: mannuronate-specific alginate lyase

This is an abbreviated version!
For detailed information about mannuronate-specific alginate lyase, go to the full flat file.

Word Map on EC 4.2.2.3

Reaction

R2-beta-D-mannuronic acid-R1
=
R2-OH
 +
4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-R1

Synonyms

(poly alpha-l-guluronate) lyase, A1 alginate lyase, A1-II, A1-III, A1-IV', A1m, A1mU, A9mC, A9mL, A9mT, AAlyase, AkAly30, AL2, alg, ALG-5, Alg-A, Alg17C, Alg2A, Alg7D, AlgA, AlgB, AlgE7, AlgI, alginase I, alginate lyase, alginate lyase A, alginate lyase A1-II, alginate lyase A1-II', alginate lyase A1-III, alginate lyase AlyPEEC, alginate lyase Atu3025, alginate lyase B, alginate lyase C, alginate lyase I, alginate lyase VI, alginate lyase1-III, AlgL, ALY, ALY-1, Aly-SJ02, Aly1, Aly2, Aly28, Aly30, Aly32, Aly33, Aly35, Aly7B, AlyA, AlyA1, AlyA2, AlyA3, AlyA5, AlyB, AlyDW11, ALYIII, alyPEEC, AlyPI, AlyPM, AlyQ, AlyV5, AlyVI, Atu3025, AXE80_11190, CL2, EC 4.2.99.4, endo-type alginate lyase, endolytic poly(M) lyase, endolytic polymannuronate lyase, exotype alginate lyase, HdAlex, hdalex-1, HdAly, KJ-2 alginate lyase, LbAly28, lyase AlyA, lyase, alginate, Lysis protein, M block-specific polymannuronate lyase, mannuronate alginate lyase, MJ-3 alginate lyase, More, MY04_2544, NIS_0185, Oal17A, oligoalginate lyase, PL-5 alginate lyase, PM lyase, poly(1,4-beta-D-mannuronide) lyase, poly(beta-D-1,4-mannuronide) lyase, Poly(beta-D-mannuronate) lyase, poly(M) lyase, poly(M)lyase, Poly(mana) alginate lyase, poly(mana)alginate lyase, polymannuronate lyase, polyMG-specific alginate lyase, protein PA1167, SP2, V12B01_24254, V12B01_24259, WP_053404615

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.2 Acting on polysaccharides
                4.2.2.3 mannuronate-specific alginate lyase

pH Stability

pH Stability on EC 4.2.2.3 - mannuronate-specific alginate lyase

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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 11
-
the pH range at which 90% or more activity of the enzymes remains s pH 3.0-11.0
703781
4 - 10
4 - 11
-
at 25 °C for 20 min, the purified enzyme retains more than 50% activity at pH 7.0-10, aly-SJ02 is most stable at pH 8.0
716092
4 - 8
-
stable
34074
5
-
relatively stable at alkaline pH, unstable below pH 5.0
34064
5 - 10.5
30°C, 30 min
664733
5 - 7
5 - 8
2 h, at least 80% residual activity
746840
5 - 9
5.5 - 8.5
AB489222
purified enzyme, 12 h, over 60% activity within this pH range remains pH 5.5 to 8.5, mostly stable at pH 7.5, half inactivation at pH 9.5
729531
5.5 - 9
24 h, more than 60% resiudal activity
748532
6 - 10
A1m is quite stable in incubation at 30°C for 1 h between pH 6.0 and 9.0. When the optimal pH in the presence of 0.2 M NaCl is shifted to pH 9.0, the activity is more than 10times compared with that at pH 9 in the absence of NaCl. Similar results are observed in 100 mM borate buffer with or without 0.2 M NaCl. The stability of A1m in various pH buffers is unaffected by adding NaCl
703603
6 - 7.5
-
stable
677288
6 - 9
6.5 - 8.5
-
30°C, 5 min, stable
34078
7
-
optimal stability
34062
7 - 10
isoform A9mT is stable at pH 7.0-10.0 in various 20 mM buffers without NaCl after incubation at 30°C for 15 min
705481
7 - 9
8
-
most stable at pH 8.0
716092
8 - 9
the purified recombinant His-tagged enzyme exhibits stable and high activity at pH between 8.0 and 9.0
729058
9.5 - 10
the relative activities at pH 9.5 and 10 in glycine-NaOH buffer are approximately 50% and 20%, respectively, of the maximal activity of isoform A9mL
705481