3.6.5.5: dynamin GTPase

This is an abbreviated version!
For detailed information about dynamin GTPase, go to the full flat file.

Reaction

Synonyms

AtDRP1A, B-dynamin , caSey1p, D100 , dDyn, DLC1, DLP-1, DLP1, DlpA, DlpB, DlpC, Dnm1, DNM1L, DNM2, DNM3, DRP, Drp1, Drp1-long, Drp1-short, DRP1E, DRP5A, Drp6, DrpB, Dyn, Dyn I, Dyn-1, Dyn1, DYN2, Dyn2aa, Dyn2ab, Dyn2ba, Dyn2bb, Dyn3, dynamin, dynamin 1, dynamin 1 GTPase, dynamin 1aa, dynamin 2, dynamin 2aa, dynamin 3, dynamin 3aa, Dynamin BREDNM19 , dynamin family GTPase, dynamin GTPase, dynamin I, dynamin I GTPase, dynamin I guanosine triphosphatase, Dynamin UDNM , Dynamin, brain , Dynamin, testicular , dynamin-1, dynamin-1 GTPase, dynamin-1-like GTPase, dynamin-1-like protein, dynamin-2, dynamin-3, dynamin-like GTPase, dynamin-like guanosine triphosphatase, dynamin-like guanosine-triphosphate hydrolase, dynamin-like myxovirus resistance protein A, dynamin-like protein 1, dynamin-like protein 6, dynamin-related GTPase, dynamin-related protein, dynamin-related protein 1, dynamin-related protein 1 isoform 3, dynamin-related protein 1A, dynamin-related protein 1E, dynamin-related protein 2A, dynamin-related protein B, dynamin-retated GTPase Drp1, dynamin1, dynamin2, dynamin2 GTPase, dynamine-related GTPase, dynein light chain 1, dynI, dynI GTPase, EC 3.6.1.50, fission dynamin, GTP phosphohydrolase, GTPase, GTPase dynamin, GTPase dynamin-1, GTPase dynamin-related protein 1, guanine triphosphatase, guanosine 5'-triphosphatase, guanosine triphosphatase, hDrp1 isoform 3, large GTPase, Mfn1, mitochondrial division dynamin, mitochondrial dynamin, mitochondrial inner membrane fusion dynamin Mgm1, mitofusin 1, mOPA1, multidomain GTPase, Mx GTPase, MX1, MxA, myxovirus resistance GTPase, neurolastin, Nicotiana tabacum dynamin-related protein 3, NtDRP3, OPA1, optic atrophy 1, PfDYN2, phosphatase, guanosine tri-, Protein shibire, ribosomal GTPase, Sey1p, Shibire protein , T-dynamin , Vps1p

ECTree

     3 Hydrolases

         3.6 Acting on acid anhydrides

             3.6.5 Acting on GTP to facilitate cellular and subcellular movement

                3.6.5.5 dynamin GTPase

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Results	in table
61	Activating Compound
13562	AA Sequence
6	Application
1	CAS Registry Number
54	Cloned(Commentary)
6	Crystallization (Commentary)
2621	Disease/ Diagnostics
91	General Information
124	IC50 Value
252	Inhibitors
43	KM Value [mM]
120	Localization
22	Metals/Ions
16	Molecular Weight [Da]
115	Natural Substrates/ Products (Substrates)
1	Organic Solvent Stability
147	Organism
114	PDB ID
18	pH Optimum
1	pI Value
4	Posttranslational Modification
117	Protein Variants
39	Purification (Commentary)
3	Reaction
18	Reaction Type
138	Reference
2	Renatured (Commentary)
105	Source Tissue
2	Specific Activity [micromol/min/mg]
186	Substrates and Products (Substrate)
42	Subunits
278	Synonyms
1	Systematic Name
20	Temperature Optimum [°C]
1	Temperature Range [°C]
26	Turnover Number [1/s]

Activating Compound

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Activating Compound on EC 3.6.5.5 - dynamin GTPase

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ACTIVATING COMPOUND

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

1-O-(1,2-di-O-palmitoyl-sn-glycerol-3-benzyloxyphosphoryl)-D-myo-inositol 3,4,5-triphosphate

2 entries

actin

2 entries

amphiphysin

Bos taurus

-

stimulation of dynamin GTPase activity

670136

-

anionic liposome

Bos taurus

-

potent stimulation of dynamin GTPase activity at low ionic strength, activator interacts with the highly basic C-terminal proline/arginine-rich domain of dynamin via ionic interactions that are essentially abrogated at physiologic ionic strength

659859

-

antibodies

Homo sapiens

-

stimulate by crosslinking through the C-terminal proline-rich domain of dynamin

644189

-

Arc/Arg3.1

2 entries

-

cardiolipin

3 entries

endogenouse rat brain vesicles

Rattus norvegicus

-

cooperative interactions between dynamin molecules are responsible for the apparent activation of GTPase observed

644188

-

Grb2

2 entries

-

ionomycin

Homo sapiens

O00429

a calcium ionophore, causes rapid mitochondrial accumulation of actin filaments followed by Drp1 accumulation at the fission site, and increases fission rate

756570

L-phosphatidylserine

Mus musculus

-

binds the pleckstrin homology domain of dynamin and enhances its GTPase activity

696551

-

lipid tubule

Rattus norvegicus

-

stimulate GTPase activity

670674

-

liposome

Homo sapiens

Q05193

liposome-stimulated GTPase activity of Dyn1

756591

-

Mff protein

Homo sapiens

O00429

mitocjondrial protein Mff alone causes only a slight increase in Drp1 GTPase activity at the concentrations tested, while the combination of Mff and actin filaments causes a substantial increase in Drp1 activity, far beyond the additive effects of either Mff or actin alone

756570

-

Mff-proteoliposome

2 entries

-

microtubule

2 entries

-

microtubules

4 entries

-

mitochondrial fission factor

2 entries

-

phosphatidylglycerol

Rattus norvegicus

-

stimulates

644187

phosphatidylinositol

Rattus norvegicus

-

stimulates

644187

phosphatidylinositol 3,4-bisphosphate

Bos taurus

-

weak activation, binds to the pleckstrin homology domain

644191

phosphatidylinositol 4,5-bisphosphate

Bos taurus

-

activates the GTPase activity of dynamin, specific for D-5 phosphoinositides, activation mechanism, activates also in the presence of 100-130 mM NaCl, the interactions are mediated by the dynamin pleckstrin homology domain

659859

phosphatidylinositol-4,5-bisphosphate

4 entries

phosphatidylserine

Rattus norvegicus

-

stimulates

644187, 644190

phosphatidylserine liposomes

Ovis aries

-

-

689237

-

phospholipase D

Rattus norvegicus

-

PLD function as a GTPase activating protein, GAP, through its phox homology domain, PX, which directly activates the GTPase domain of dynamin

682064

-

Phospholipid

5 entries

SH3 domain-containing protein

Bos taurus

-

activates the GTPase activity of dynamin, activation mechanism

659859

-

SH3 domains

2 entries

-

additional information

14 entries

-

1-O-(1,2-di-O-palmitoyl-sn-glycerol-3-benzyloxyphosphoryl)-D-myo-inositol 3,4,5-triphosphate

Bos taurus

-

activates, binds to the pleckstrin homology domain

644191

1-O-(1,2-di-O-palmitoyl-sn-glycerol-3-benzyloxyphosphoryl)-D-myo-inositol 3,4,5-triphosphate

Bos taurus

-

activates the GTPase activity of dynamin, specific for D-5 phosphoinositides, activation mechanism, activates also in the presence of 100-130 mM NaCl, the interactions are mediated by the dynamin pleckstrin homology domain

659859

actin

Homo sapiens

O00429

actin filaments bind purified Drp1 and increase GTPase activity in a manner that is synergistic with the mitochondrial protein Mff, suggesting a role for direct Drp1/actin interaction

756570

actin

Homo sapiens

O00429

actin filaments increase Drp1's GTPase activity eith a 3.5fold increase. The assembly of filaments of actin is the signal to Drp1 to accumulate on mitochondria and for fission

756570

Arc/Arg3.1

Rattus norvegicus

P21575, P39052, Q08877

enhances dynamin polymerization and GTPase activity. The activity-regulated cytoskeleton-associated protein, Arc, is an immediate-early gene product implicated in various forms of synaptic plasticity. Arc promotes endocytosis of AMPA type glutamate receptors and regulates cytoskeletal assembly in neuronal dendrites. Bacterially-expressed His6-Arc facilitates the polymerization of Dyn2 and stimulates its GTPase activity under physiologic conditions (37°C and 100 mM NaCl). At lower ionic strength Arc also stabilizes preformed Dyn2 polymers against GTP-dependent disassembly, thereby prolonging assembly-dependent GTP hydrolysis catalyzed by Dyn2. Arc also increases the GTPase activity of Dyn3, an isoform of implicated in dendrite remodeling, but does not affect the activity of Dyn1, a neuron-specific isoform involved in synaptic vesicle recycling. Analysis of recombinant full-length mouse Arc and of His6-tagged fragments, including Arc-N (residues 1-227), Arc-C (residues 228-396), and Arc-(DELTA195-214). While residues 195-214 may contribute to dynamin binding, additional binding determinants are likely to be present in the N-terminal portion of Arc. Arc stimulates the assembly-dependent GTPase activity of isozymes Dyn2 and Dyn3. The effect of Arc on GTPase activity of dynamin mirrors its effect on dynamin assembly as measured by turbidity

756073

-

Arc/Arg3.1

Rattus norvegicus

P21575, P39052, Q08877

enhances dynamin polymerization and GTPase activity. The activity-regulated cytoskeleton-associated protein, Arc, is an immediate-early gene product implicated in various forms of synaptic plasticity. Arc promotes endocytosis of AMPA type glutamate receptors and regulates cytoskeletal assembly in neuronal dendrites. Bacterially-expressed His6-Arc increases the GTPase activity of Dyn3, an isoform of implicated in dendrite remodeling. Analysis of recombinant full-length mouse Arc and of His6-tagged fragments, including Arc-N (residues 1-227), Arc-C (residues 228-396), and Arc-(DELTA195-214). Arc stimulates the assembly-dependent GTPase activity of isozymes Dyn2 and Dyn3. The effect of Arc on GTPase activity of dynamin mirrors its effect on dynamin assembly as measured by turbidity

756073

-

cardiolipin

Homo sapiens

O00429

Drp1 binding to anionic lipids such as cardiolipin increases its GTPase activity

756570

cardiolipin

Homo sapiens

O00429

stimulates Drp1, synergistically with mitochondrial fission factor

757169

cardiolipin

Rattus norvegicus

O35303

stimulates Drp1, synergistically with mitochondrial fission factor

757169

Grb2

Bos taurus

-

activates, binds to carboxyl-terminal proline/arginine-rich domain. Grb2 and phosphatidylinositol 4,5-bisphosphate together increase the dynamin GTPase activity up to 4fold higher than that obtained by these activators tested separately

644191

-

Grb2

Rattus norvegicus

-

stimulates

644197

-

Mff-proteoliposome

Homo sapiens

O00429

lipid-stimulated GTPase activity

757169

-

Mff-proteoliposome

Rattus norvegicus

O35303

lipid-stimulated GTPase activity

757169

-

microtubule

Bos taurus

-

potent stimulation of dynamin GTPase activity at low ionic strength, activator interacts with the highly basic C-terminal proline/arginine-rich domain of dynamin via ionic interactions that are essentially abrogated at physiologic ionic strength

659859

-

microtubule

Homo sapiens

-

activates GTPase activity

659560

-

microtubules

Bos taurus

-

activates, binds to carboxyl-terminal proline/arginine-rich domain

644191

-

microtubules

Rattus norvegicus

-

stimulates

644197

-

microtubules

Rattus norvegicus

-

cooperative interactions between dynamin molecules are responsible for the apparent activation of GTPase observed

644188

-

microtubules

Rattus norvegicus

-

accelerates GTPase activity up to 150fold, about 4fold activation of ATPase activity

644195

-

mitochondrial fission factor

Homo sapiens

O00429

Mff, stimulates Drp1 independently of, but synergistically with cardiolipin

757169

-

mitochondrial fission factor

Rattus norvegicus

O35303

Mff, stimulates Drp1 independently of, but synergistically with cardiolipin

757169

-

phosphatidylinositol-4,5-bisphosphate

Bos taurus

-

activates

644184

phosphatidylinositol-4,5-bisphosphate

Bos taurus

-

best activator, binds to the pleckstrin homology domain. Grb2 and phosphatidylinositol 4,5-bisphosphate together increase the dynamin GTPase activity up to 4fold higher than that obtained by these activators tested separately

644191

phosphatidylinositol-4,5-bisphosphate

Homo sapiens

-

activates

644184

phosphatidylinositol-4,5-bisphosphate

Mus musculus

-

binds the pleckstrin homology domain of dynamin and enhances its GTPase activity

696551

Phospholipid

Homo sapiens

-

mixed-lineage kinase 2-SH2 domain binds dynamin and greatly enhances activation of GTPase by phospholipid

644183

Phospholipid

Ovis aries

-

mixed-lineage kinase 2-SH2 domain binds dynamin and greatly enhances activation of GTPase by phospholipid

644183

Phospholipid

Ovis aries

-

stimulates GTPase activity of dynamin

697337

Phospholipid

Rattus norvegicus

-

acidic phospholipids, cooperative interactions between dynamin molecules are responsible for the apparent activation of GTPase observed

644188

Phospholipid

Rattus norvegicus

-

mixed-lineage kinase 2-SH2 domain binds dynamin and greatly enhances activation of GTPase by phospholipid

644183

SH3 domains

Rattus norvegicus

-

stimulates

644197

-

SH3 domains

Rattus norvegicus

-

stimulation by several recombinant SH3 domains, binding through proline-rich sequence motifs

644194

-

additional information

Bos taurus

-

dynamin is activated by the facilitation of its self-association, GTPase activity is stimulated by any factor that induces the formation of appropriately oriented dynamin polymers, not activated by phosphatidylinositol 4-phosphate or 1-O-(1,2-di-O-palmitoyl-sn-glycerol-3-benzyloxyphosphoryl)-D-myo-inositol 3,4-diphosphate

659859

-

additional information

Bos taurus

-

oligomerization of dynamin stimulates its GTPase activity

659996

-

additional information

Homo sapiens

-

dynamin can self-assemble forming higher order structures such as rings and spirals that exhibit up to 100fold stimulated GTPase activity, conformational changes in the active site accompany self-assembly

659389

-

additional information

Homo sapiens

-

robust stimulation of dynamin's GTPase activity upon polymerization

720524

-

additional information

Homo sapiens

O00429

interchangeable adaptors regulate mitochondrial dynamin assembly for membrane scission

758217

-

additional information

Mus musculus

-

robust stimulation of dynamin's GTPase activity upon polymerization

720524

-

additional information

Rattus norvegicus

-

dynamin I and II are stimulated by self-assembly, dynamin II has a greater propensity for self-assembly than neuronal dynamin I

644197

-

additional information

Rattus norvegicus

-

the GAP function of PLD1-PX stimulates the GTPase activity of dynamin by increasing dynamin self-assembly

682064

-

additional information

Rattus norvegicus

-

robust stimulation of dynamin's GTPase activity upon polymerization

720524

-

additional information

Rattus norvegicus

P21575

activity-regulated cytoskeleton-associated protein, Arc (Arc/Arg3.1) is an immediate-early gene product implicated in various forms of synaptic plasticity. Arc promotes endocytosis of AMPA type glutamate receptors and regulates cytoskeletal assembly in neuronal dendrites. Bacterially-expressed His6-Arc does not affect the activity of Dyn1, a neuron-specific isoform involved in synaptic vesicle recycling, in contrast to the other Dyn isozymes, Arc stimulates the assembly-dependent GTPase activity of Dyn2 and Dyn3. Analysis of recombinant full-length mouse Arc and of His6-tagged fragments, including Arc-N (residues 1-227), Arc-C (residues 228-396), and Arc-(DELTA195-214)

756073

-

additional information

Rattus norvegicus

P39052

activity-regulated cytoskeleton-associated protein, Arc (Arc/Arg3.1) is an immediate-early gene product implicated in various forms of synaptic plasticity. Arc promotes endocytosis of AMPA type glutamate receptors and regulates cytoskeletal assembly in neuronal dendrites. Bacterially-expressed His6-Arc does not affect the activity of Dyn1, a neuron-specific isoform involved in synaptic vesicle recycling, in contrast to the other Dyn isozymes, Arc stimulates the assembly-dependent GTPase activity of Dyn2 and Dyn3. Analysis of recombinant full-length mouse Arc and of His6-tagged fragments, including Arc-N (residues 1-227), Arc-C (residues 228-396), and Arc-(DELTA195-214)

756073

-

additional information

Rattus norvegicus

Q08877

activity-regulated cytoskeleton-associated protein, Arc (Arc/Arg3.1) is an immediate-early gene product implicated in various forms of synaptic plasticity. Arc promotes endocytosis of AMPA type glutamate receptors and regulates cytoskeletal assembly in neuronal dendrites. Bacterially-expressed His6-Arc does not affect the activity of Dyn1, a neuron-specific isoform involved in synaptic vesicle recycling, in contrast to the other Dyn isozymes, Arc stimulates the assembly-dependent GTPase activity of Dyn2 and Dyn3. Analysis of recombinant full-length mouse Arc and of His6-tagged fragments, including Arc-N (residues 1-227), Arc-C (residues 228-396), and Arc-(DELTA195-214)

756073

-

additional information

Saccharomyces cerevisiae

P54861

interchangeable adaptors regulate mitochondrial dynamin assembly for membrane scission

758217

-

additional information

Saccharomyces cerevisiae

-

interchangeable adaptors regulate mitochondrial dynamin assembly for membrane scission

758217

-