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3.6.5.5: dynamin GTPase

This is an abbreviated version!
For detailed information about dynamin GTPase, go to the full flat file.

Word Map on EC 3.6.5.5

Reaction

GTP
+
H2O
=
GDP
 +
phosphate

Synonyms

AtDRP1A, B-dynamin , caSey1p, D100 , dDyn, DLC1, DLP-1, DLP1, DlpA, DlpB, DlpC, Dnm1, DNM1L, DNM2, DNM3, DRP, Drp1, Drp1-long, Drp1-short, DRP1E, DRP5A, Drp6, DrpB, Dyn, Dyn I, Dyn-1, Dyn1, DYN2, Dyn2aa, Dyn2ab, Dyn2ba, Dyn2bb, Dyn3, dynamin, dynamin 1, dynamin 1 GTPase, dynamin 1aa, dynamin 2, dynamin 2aa, dynamin 3, dynamin 3aa, Dynamin BREDNM19 , dynamin family GTPase, dynamin GTPase, dynamin I, dynamin I GTPase, dynamin I guanosine triphosphatase, Dynamin UDNM , Dynamin, brain , Dynamin, testicular , dynamin-1, dynamin-1 GTPase, dynamin-1-like GTPase, dynamin-1-like protein, dynamin-2, dynamin-3, dynamin-like GTPase, dynamin-like guanosine triphosphatase, dynamin-like guanosine-triphosphate hydrolase, dynamin-like myxovirus resistance protein A, dynamin-like protein 1, dynamin-like protein 6, dynamin-related GTPase, dynamin-related protein, dynamin-related protein 1, dynamin-related protein 1 isoform 3, dynamin-related protein 1A, dynamin-related protein 1E, dynamin-related protein 2A, dynamin-related protein B, dynamin-retated GTPase Drp1, dynamin1, dynamin2, dynamin2 GTPase, dynamine-related GTPase, dynein light chain 1, dynI, dynI GTPase, EC 3.6.1.50, fission dynamin, GTP phosphohydrolase, GTPase, GTPase dynamin, GTPase dynamin-1, GTPase dynamin-related protein 1, guanine triphosphatase, guanosine 5'-triphosphatase, guanosine triphosphatase, hDrp1 isoform 3, large GTPase, Mfn1, mitochondrial division dynamin, mitochondrial dynamin, mitochondrial inner membrane fusion dynamin Mgm1, mitofusin 1, mOPA1, multidomain GTPase, Mx GTPase, MX1, MxA, myxovirus resistance GTPase, neurolastin, Nicotiana tabacum dynamin-related protein 3, NtDRP3, OPA1, optic atrophy 1, PfDYN2, phosphatase, guanosine tri-, Protein shibire, ribosomal GTPase, Sey1p, Shibire protein , T-dynamin , Vps1p

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.5 Acting on GTP to facilitate cellular and subcellular movement
                3.6.5.5 dynamin GTPase

Cloned

Cloned on EC 3.6.5.5 - dynamin GTPase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a 6-His N-terminal-tagged human dynamin2, lacking the C-terminal proline-rich domain, is constructed, cloned into the pET28a vector for expression in Escherichia coli BL21DE3 cells
-
a CHO-K1 cDNA library in the pSPORT vector is screened, the PCR product, derived from a positive clone, is cloned using the vectors pcDNA3.1Zeo+Myc6-, pcDNA3.1Zeo+Flag-, pcDNA3.1Zeo+HA2-, and pGEX6P-2, DLP1 cDNA from ZP121 is subcloned into pGEM-Teasy
-
adenovirus expressing the dynamin mutant K44A is constructed
-
DNA encoding the full-length Dnm1 protein is cloned into the pMALc2x vector for expression in Escherichia coli Rosetta cells
-
Dyn1 expression in bovine adrenal chromaffin cells, coexpression with granule marker VMAT2-pH, the amount of dynamin on the plasma membrane in dynamin-transfected cells was increased 2.5 to 3.5 times compared with endogenous dynamin in nontransfected cells
-
dynamin 2 genotyping
expressed in a fibroblastoid cell line and in embryonic stem cells
expressed in CHO cells
-
expressed in HEK-293 cells
-
expressed in HeLa cells
expressed in umbilical cord blood CD34+ cells
expressed in Vero cells
expression in COS-7 cells
-
expression of dynamin II in Tn5 cells
-
expression of GFP-tagged or V5/His-tagged wild-type and K44A mutant dynamin 2aa in HeLa cells
-
expression of GST-tagged wild-type and mutant GST-SEY1 in Escherichia coli strain BL21
-
expression of His-tagged DLP-1 isozyme 2 in Escherichia coli strain C41 (DE3)
-
expression of wild-type and K44A mutant dynamin in HeLa cells
-
expression of wild-type and K44A mutant dynamin-1 in HeLa cells and in Sf9 cells
-
for identification of the EDR3 gene a cosmid library using BAC F27H5 DNA is constructed, for investigation of the subcellular localization a green fluorescent protein fusion to the N-terminus of DRP1E is used
-
gene Dnm1, recombinant expression of C-terminally His6-tagged isozyme Dyn1 in Spodoptera frugiperda Sf9 cells
gene DNM1, recombinant expression of Drp1 containing an N-terminal PreScission protease cleavage site and a FLAG-One-Strep-tag (IBA) from plasmid pRS415-DNM1 in Saccharomyces cerevisiae strain JSY9612 lacking all fission proteins (MATa, can1, ade2, trp1, ura3, his3, leu2, pep4::HIS3, prb1::LEU2, bar1::HISG, lys2::GAL1/10-GAL4), coexpression with Saccharomyces cerevisiae mitochondrial fission 1 protein, Fis1 (UniProt ID P40515)
gene DNM1, recombinant expression of the dynamin 1 GTPase domain GG1 from modified pGEX-4T1 vector containing a TEV protease site. The fragments are connected by a linker composed of eight amino acid residues (KHGTDSRV) in Escherichia coli strain BL21 (DE3)
gene DNM2, enzyme expression analysis
gene DNM2, enzyme expression analysis, recombinant expression of GFP-tagged isozyme dynamin2 in human T24 cells
gene Dnm2, recombinant expression of C-terminally His6-tagged isozyme Dyn2 in Spodoptera frugiperda Sf9 cells
gene Dnm3, recombinant expression of C-terminally His6-tagged isozyme Dyn3 in Spodoptera frugiperda Sf9 cells
gene DNMTL1, recombinant expression of the splice variants of Drp1 in Escherichia coli strain BL21 Star (DE3)
gene for dynamin1, expression in HEK293-cre4 cells
-
gene Rnf112, recombinant expression of C-terminally HA-tagged enzyme in transgenic C83S/C103S mutant mice and in HeLa cells, and of GFP-tagged enzyme from pEGFP-N1 vector in transgenic mice
His-tagged dynamin I is expressed in Escherichia coli Rosetta2 (DE3) cells
-
human dynamin is expressed in SF9 insect cells, using the Bac-to-Bac baculovirus expression system a full-length cDNA encoding human dynamin1 containing a 6His-tag is subcloned into pFastBac, a bacmid is generated after transposition in Escherichia coli
-
into the pFLAG-CMV-2 vector, five functional dynamin domains, GTPase, Middle, pleckstrin homology, GTPase effector and proline rich, are subcloned into pGEX-4T2
-
into the pT-Adv vector, into pET-15b for expression in Escherichia coli BL21DE3codon-plus cells, a 711 bp fragment corresponding to the GTPase N-terminal domain into pET-22b, and into the pSynXIV VI+X3 vector for recombinat expression in the BEV system
overexpression in Chineses hamster ovary cells
-
overexpression of human neuronal dynamin in Sf9 insect cells
-
recombinant expression of C-terminally His6-tagged wild-type and mutants of DNM1L isoform 2
recombinant expression of GST- and His-tagged MxA in Escherichia coli strain BL21(DE3)
recombinant expression of HA-tagged neurolastin in HEK cells, recombinant expression of GST-tagged wild-type and mutant enzymes in HeLa cells
-
recombinant expression of His6-tagged or FLAG-tagged wild-type and mutant enzymes in HeLa cells
recombinant expression of N-terminally Strep-tagged Drp1 with an HRV3C protease site from pET16b vector in Escherichia coli strain BL21 Star (DE3), stable recombinant expression of GFP-tagged DrpI in U2OS cells with partial shRNA suppression of endogenous Drp1 (gDrp-U2OS), GFP-Drp1 functionally compensates for endogenous Drp1 in these cells
recombinant expression of nucleotide-free GTPase-GTPase effector domain fusion protein of enzyme Drp1, and wild-type and mutant full-length enzymes in Escherichia coli strain BL21(DE3)
recombinant expression of the GST-tagged chimeric fusion protein of AtDRP1A fusion protein in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
recombinant expression of truncated enzyme Sey1p(1-692) in Escherichia coli, recombinant expressioj of Myc-tagged scSey1p in COS-7 cells and immunolocalization study
the cDNA of rat Drp1 is subcloned into pEGFP-N1 and p3xFLAG-CMV-10 for transfection of HeLa cells, and in pET28a for expression in Escherichia coli
-
the full-length mouse dynamin-1 cDNA isolated from retina is subcloned into the pGEX-2TK vector, the construct is used to generate three different dynamin-1 domain constructs, the N-terminal domain, aa 1-520, the proline-rich domain, aa 750-814, and the C-terminal domain, aa 521-814
-
the vectors pcDNA3.1 and pADT3T7tet are used, recombinant adenoviruses are produced in HEK293-Cre cells
wild-type and mutant dynamin is expressed using the baculovirus pBac 4 system, expression in COS-7 cells
-
wild-type mOPA1 and its mutants are subcloned into the mammalian expression vector pCNX2 for transfection of COS-7 cells