Literature summary for 3.6.5.5 extracted from

Marks, B.; Stowell, M.H.B.; Vallis, Y.; Mills, I.G.; Gibson, A.; Hopkins, C.R.; McMahon, H.T.
GTPase activity of dynamin and resulting conformation change are essential for endocytosis (2001), Nature, 410, 231-235.

Search for more literature for 3.6.5.5

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	oligomerization of dynamin stimulates its GTPase activity	Bos taurus

Cloned(Commentary)

Cloned (Comment)	Organism
wild-type and mutant dynamin is expressed using the baculovirus pBac 4 system, expression in COS-7 cells	Bos taurus

Protein Variants

Protein Variants	Comment	Organism
K142A	mutation in the GTPase domain of dynamin, mutant has a moderately impaired GTPase activity, kinetics	Bos taurus
additional information	point mutants of the GTPase effector domain of dynamin	Bos taurus
R66A	mutation in the GTPase domain of dynamin, kinetics	Bos taurus
S45N	mutation in the GTPase domain of dynamin, mutant is defective in GTP binding, kinetics	Bos taurus
T141Q	mutation in the GTPase domain of dynamin, mutant is partially inhibited in its GTPase activity, kinetics	Bos taurus
T65A	mutation in the GTPase domain of dynamin, very low GTPase activity, kinetics	Bos taurus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0067	-	GTP	K142A mutant of the GTPase domain of dynamin	Bos taurus
0.0076	-	GTP	T141Q mutant of the GTPase domain of dynamin	Bos taurus
0.0078	-	GTP	wild-type dynamin	Bos taurus
0.0091	-	GTP	T65A mutant of the GTPase domain of dynamin	Bos taurus
0.065	-	GTP	R66A mutant of the GTPase domain of dynamin	Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
GTP + H2O	Bos taurus	involved in clathrin-mediated endocytosis and other vesicular trafficking processes, dynamin has a mechanochemical function in vesicle scission, the GTPase activity of dynamin and the resulting conformation change are essential for endocytosis	GDP + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	dynamin 1	-

Purification (Commentary)

Purification (Comment)	Organism
-	Bos taurus

Reaction

Reaction	Comment	Organism	Reaction ID
GTP + H2O = GDP + phosphate	an enzyme with a molecular mass of about 100 kDa that is involved in endocytosis and is instrumental in pinching off membrane vesicles	Bos taurus	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GTP + H2O	involved in clathrin-mediated endocytosis and other vesicular trafficking processes, dynamin has a mechanochemical function in vesicle scission, the GTPase activity of dynamin and the resulting conformation change are essential for endocytosis	Bos taurus	GDP + phosphate	-	?
GTP + H2O	GTP hydrolysis results in conformational changes in dynamin bound to lipid nanotubes	Bos taurus	GDP + phosphate	-	?

Subunits

Subunits	Comment	Organism
More	oligomerization of dynamin stimulates its GTPase activity	Bos taurus

Synonyms

Synonyms	Comment	Organism
dynamin 1	GTPase activity	Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
additional information	-	assay at room temperature	Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.023	-	GTP	T65A mutant of the GTPase domain of dynamin	Bos taurus
0.083	-	GTP	S45N mutant of the GTPase domain of dynamin	Bos taurus
0.45	-	GTP	R66A mutant of the GTPase domain of dynamin	Bos taurus
0.55	-	GTP	T141Q mutant of the GTPase domain of dynamin	Bos taurus
1.8	-	GTP	K142A mutant of the GTPase domain of dynamin	Bos taurus
3.1	-	GTP	wild-type dynamin	Bos taurus

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Release 2023.1 (January 2023)