Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | oligomerization of dynamin stimulates its GTPase activity | Bos taurus |
Cloned (Comment) | Organism |
---|---|
wild-type and mutant dynamin is expressed using the baculovirus pBac 4 system, expression in COS-7 cells | Bos taurus |
Protein Variants | Comment | Organism |
---|---|---|
K142A | mutation in the GTPase domain of dynamin, mutant has a moderately impaired GTPase activity, kinetics | Bos taurus |
additional information | point mutants of the GTPase effector domain of dynamin | Bos taurus |
R66A | mutation in the GTPase domain of dynamin, kinetics | Bos taurus |
S45N | mutation in the GTPase domain of dynamin, mutant is defective in GTP binding, kinetics | Bos taurus |
T141Q | mutation in the GTPase domain of dynamin, mutant is partially inhibited in its GTPase activity, kinetics | Bos taurus |
T65A | mutation in the GTPase domain of dynamin, very low GTPase activity, kinetics | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0067 | - |
GTP | K142A mutant of the GTPase domain of dynamin | Bos taurus | |
0.0076 | - |
GTP | T141Q mutant of the GTPase domain of dynamin | Bos taurus | |
0.0078 | - |
GTP | wild-type dynamin | Bos taurus | |
0.0091 | - |
GTP | T65A mutant of the GTPase domain of dynamin | Bos taurus | |
0.065 | - |
GTP | R66A mutant of the GTPase domain of dynamin | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | Bos taurus | involved in clathrin-mediated endocytosis and other vesicular trafficking processes, dynamin has a mechanochemical function in vesicle scission, the GTPase activity of dynamin and the resulting conformation change are essential for endocytosis | GDP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
dynamin 1 | - |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
GTP + H2O = GDP + phosphate | an enzyme with a molecular mass of about 100 kDa that is involved in endocytosis and is instrumental in pinching off membrane vesicles | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | involved in clathrin-mediated endocytosis and other vesicular trafficking processes, dynamin has a mechanochemical function in vesicle scission, the GTPase activity of dynamin and the resulting conformation change are essential for endocytosis | Bos taurus | GDP + phosphate | - |
? | |
GTP + H2O | GTP hydrolysis results in conformational changes in dynamin bound to lipid nanotubes | Bos taurus | GDP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | oligomerization of dynamin stimulates its GTPase activity | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
dynamin 1 | GTPase activity | Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
assay at room temperature | Bos taurus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
GTP | T65A mutant of the GTPase domain of dynamin | Bos taurus | |
0.083 | - |
GTP | S45N mutant of the GTPase domain of dynamin | Bos taurus | |
0.45 | - |
GTP | R66A mutant of the GTPase domain of dynamin | Bos taurus | |
0.55 | - |
GTP | T141Q mutant of the GTPase domain of dynamin | Bos taurus | |
1.8 | - |
GTP | K142A mutant of the GTPase domain of dynamin | Bos taurus | |
3.1 | - |
GTP | wild-type dynamin | Bos taurus |